Nutrient enrichment, propagule pressure, and herbivory interactively influence the competitive ability of an invasive alien macrophyte Myriophyllum aquaticum.

Introduction: Freshwater ecosystems are susceptible to invasion by alien macrophytes due to their connectivity and various plant dispersal vectors. These ecosystems often experience anthropogenic nutrient enrichment, favouring invasive species that efficiently exploit these resources. Propagule pressure (reflecting the quantity of introduced individuals) and habitat invasibility are key determinants of invasion success. Moreover, the enemy release hypothesis predicts that escape from natural enemies, such as herbivores, allows alien species to invest more resources to growth and reproduction rather than defense, enhancing their invasive potential. Yet, the combined impact of propagule pressure, herbivory, and nutrient enrichment on the competitive dynamics between invasive alien macrophytes and native macrophyte communities is not well understood due to a paucity of studies. Methods: We conducted a full factorial mesocosm experiment to explore the individual and combined effects of herbivory, nutrient levels, propagule pressure, and competition on the invasion success of the alien macrophyte Myriophyllum aquaticum into a native macrophyte community comprising Vallisneria natans, Hydrilla verticillata, and Myriophyllum spicatum. This setup included varying M. aquaticum densities (low vs. high, simulating low and high propagule pressures), two levels of herbivory by the native snail Lymnaea stagnalis (herbivory vs no-herbivory), and two nutrient conditions (low vs. high). Myriophyllum aquaticum was also grown separately at both densities without competition from native macrophytes. Results: The invasive alien macrophyte M. aquaticum produced the highest shoot and total biomass when simultaneously subjected to conditions of high-density intraspecific competition, no herbivory, and low-nutrient availability treatments. Moreover, a high propagule pressure of M. aquaticum significantly reduced the growth of the native macrophyte community in nutrient-rich conditions, but this effect was not observed in nutrient-poor conditions. Discussion: These findings indicate that M. aquaticum has adaptive traits enabling it to flourish in the absence of herbivory (supporting the enemy release hypothesis) and in challenging environments such as intense intraspecific competition and low nutrient availability. Additionally, the findings demonstrate that when present in large numbers, M. aquaticum can significantly inhibit the growth of native macrophyte communities, particularly in nutrient-rich environments. Consequently, reducing the propagule pressure of M. aquaticum could help control its spread and mitigate its ecological impact. Overall, these findings emphasize that the growth and impacts of invasive alien plants can vary across different habitat conditions and is shaped by the interplay of biotic and abiotic factors.

Responses of soil phosphorus cycling and bioavailability to plant invasion in river-lake ecotones.

The invasion of exotic plants in the river-lake ecotone has seriously affected the nutrient cycling processes in wetland soil. The South American species Alternanthera philoxeroides (Mart.) Griseb. is rapidly invading the river-lake ecotone in subtropical China, and has become the dominant species in the river-lake ecotone. However, there have been few studies on the effects of A. philoxeroides invasion on soil phosphorus (P) cycling and bioavailability in this ecotone. Here, we measured the bioavailable P fractions, physicochemical properties and nutrient content in the surface soils of the native plant (Zizania latifolia (Griseb.) Turcz and Nelumbo nucifera Gaertn.) communities and the adjacent invasive A. philoxeroides communities in three river-lake ecotones with different nutrient substrates in the subtropical Dongting Lake basin over a 3-year period to reveal the effects of A. philoxeroides invasion on the morphology and concentrations of soil bioavailable P. The principal coordinate analysis results showed that the A. philoxeroides invasion significantly altered the bioavailable P concentrations in the soil of native plant communities in the different river-lake ecotones, and this effect was not disturbed by the heterogeneity of the soil matrix. However, the effects of invasion into different native plant communities on the fractions of soil bioavailable P were different. Compared with native Z. latifolia and N. nucifera communities, A. philoxeroides invasion increased the concentration of inorganic P by 39.5% and 3.7%, respectively, and the concentration of organic P decreased by 32.7% and 31.9%, respectively. In addition, the invasion promoted P cycling and accumulation in the river-lake ecotone, which resulted in average decreases in the soil N:P and C:P ratios of 7.9% and 12.5%, respectively. These results highlight the impact of exotic plant invasions on nutrient cycling in wetland ecosystems in the river-lake ecotone, and this process may be detrimental to the late recovery of native plants.

Response of spatio-temporal changes in sediment phosphorus fractions to vegetation restoration in the degraded river-lake ecotone.

Phosphorus (P) is an essential element in the ecosystem and the cause of the eutrophication of rivers and lakes. The river-lake ecotone is the ecological buffer zone between rivers and lakes, which can transfer energy and material between terrestrial and aquatic ecosystems. Vegetation restoration of degraded river-lake ecotone can improve the interception capacity of P pollution. However, the effects of different vegetation restoration types on sediment P cycling and its mechanism remain unclear. Therefore, we seasonally measured the P fractions and physicochemical properties of sediments from different restored vegetation (three native species and one invasive species). The results found that vegetation restoration significantly increased the sediment total P and bioavailable P content, which increased the sediment tolerance to P pollution in river-lake ecotone. In addition, the total P content in sediments was highest in summer and autumn, but lower in spring and winter. The total P and bioavailable P contents in surface sediments were the highest. They decreased with increasing depth, suggesting that sediment P assimilation by vegetation restoration and the resulting litter leads to redistribution of P in different seasons and sediment depths. Microbial biomass-P (MBP), total nitrogen (TN), and sediment organic matter (SOM) are the main factors affecting the change of sediment phosphorus fractions. All four plants' maximum biomass and P storage appeared in the autumn. Although the biomass and P storage of the invasive species Alternanthera philoxeroides were lower, the higher bioavailable P content and MBP values of the surface sediments indicated the utilization efficiency of sediment resources. These results suggest that vegetation restoration affects the distribution and circulation of P in river and lake ecosystems, which further enhances the ecological function of the river-lake ecotone and prevents the eutrophication and erosion of water and sediment in the river-lake ecotone.

The spatiotemporal characteristics of water quality and phytoplankton community in a shallow eutrophic lake: Implications for submerged vegetation restoration.

One of the most serious consequences of eutrophication in shallow lakes is deterioration of water quality, proliferation of phytoplankton and disappearance of submerged macrophytes. After removing herbivorous and plankti-benthivorous fish, submerged macrophyte restoration was utilized at the entire lake (82.7 km2) to combat eutrophication and improve water quality in the shallow subtropical aquaculture of Lake Datong. We conducted two years of monitoring, from March 2018 to February 2020. During the first year of restoration, 80% of the area of Lake Datong (approximately 60 km2) was successfully recovered by submerged vegetation, and the water quality was improved. For example, the phosphorous (P) content (including total P (TP), dissolved reactive P (DRP) and total dissolved P (TDP)) and turbidity decreased, and the Secchi depth (SD) increased. However, the submerged vegetation disappeared from autumn 2019 in the intermittent recovery area (MN), while the continuous recovery area (DX) continued to recover with an abundance of submerged vegetation. During the second year, the water quality continued to improve significantly in the DX area, with high biomass and coverage of submerged vegetation. In the MN area, although turbidity and ammonia nitrogen (NH4+-N) increased significantly and SD decreased significantly, the P content (TP, TDP, and DRP) still continued to decrease. The restoration of submerged macrophytes could significantly decrease the density of phytoplankton. Over time, there was a regime shift in Lake Datong. The structural equation model (SEM) results illustrated that the water level and submerged plant coverage were the primary drivers that triggered changes in the state of the lake ecosystem. Our results highlight the potential of restoring submerged vegetation to control water eutrophication at the whole-lake scale. However, the water level in spring was the primary driver that triggered changes in the state of the lake ecosystem. Water level management should be emphasized during the early stages of recovery of submerged plants.

Response of sediment and water microbial communities to submerged vegetations restoration in a shallow eutrophic lake.

Submerged macrophytes are the main primary producers in shallow lakes and play an important role in structuring communities. Aquatic microbes are also an important component of aquatic ecosystems and play important roles in maintaining the health and stability of ecosystems. However, little is known about the interactions between macrophytes and microbes during the reintroduction of submerged vegetation. Here, we chose restored zones dominated by four different submerged vegetations and a bare zone in a shallow eutrophic lake to unveil the microbial diversity, composition and structure changes in sediment and water samples after submerged macrophytes were recovered for one and a half years (July 2019) and two years (April 2020). We found that the recovery of submerged vegetations decreased phosphorus content in water and sediments but increased nitrogen and carbon content in sediments. We observed that the transparency of water in the restored zones was significantly higher than that in the bare zone in July. The recovery of submerged vegetations significantly influenced the alpha diversity of bacterial communities in sediments, with higher values observed in restored zones than in bare zones, whereas no significant influence was found in the water samples. In July, the macrophyte species showed strong effects on the bacterial community composition in water and relatively little effect in sediment. However, a strong effect of the macrophyte species on the composition of bacterial communities in sediments was observed in April, which may be related to the decomposition of plant litter and the decay of detritus. Additionally, the dissimilarity of the sedimentary bacterial community may increase more slowly with environmental changes than the planktonic bacterial community dissimilarity. These results suggest that the large-scale restoration of aquatic macrophytes can not only improve water quality and change sediment characteristics but can also affect the diversity and compositions of bacterial communities, and these effects seem to be very long-lasting.

Submerged macrophytes successfully restored a subtropical aquacultural lake by controlling its internal phosphorus loading.

Intensive aquaculture has largely changed the global phosphorus (P) flow and become one of the main reasons for the eutrophication of global aquatic ecosystem. Artificial planting submerged macrophytes has attracted enormous interest regarding the restoration of eutrophic lakes. However, few large-scale (>80 km2) studies have focused on the restoration of aquatic vegetation in the subtropical lakes, and the mechanism underlying the restrain of sediment P release by macrophytes remains unknown. In this study, field surveys and the diffusive gradients in thin films (DGT) technique were used to elucidate the effects of macrophytes on internal P loading control in a typical eutrophic aquacultural lake. Results showed that half of the P content in overlying water and sediments, particularly dissolved P in overlying water and calcium bound P (Ca-P) in sediment, were removed after restoration. Temperature, as well as dissolved oxygen (DO) and P concentration gradients near the sediment-water interface (SWI) jointly controlled the release of labile P from surface sediments. Submerged macrophytes can effectively inhibit the release of sediment P into the overlying water, which depended on DO concentration in the bottom water. Future restoration projects should focus on the temperature response of submerged macrophytes of different growth forms (especially canopy-forming species) to avoid undesirable restoration effects. Our results complement existing knowledge about submerged macrophytes repairing subtropical P-contaminated lakes and have positive significance for lake restoration by in situ phytoremediation.

Effectiveness of dredging on internal phosphorus loading in a typical aquacultural lake.

Intensive aquaculture significantly affects the global phosphorus (P) cycle and enhances eutrophication in inland waters. Sediment dredging efficiently removes P-rich sediments from shallow-water eutrophic lakes. However, studies on the effects of sediment dredging on the internal P loading of aquacultural lakes are still lacking. Moreover, the migration and transformation processes of labile P and the mechanisms of sediment P release are unclear. To evaluate dredging effectiveness, we employed two in situ high-resolution sampling techniques to simultaneously measure sediment labile P and porewater soluble reactive P (SRP) and Fe (II) at the millimeter scale. Dredging effectively reduced surface sediment CaP contents and organic matter (OM) below the sediment-water interface (SWI). Moreover, dredging decreased the SRP diffusion flux across the SWI in summer. After dredging, FeP (P bound to Fe, Al, and Mn oxides and hydroxides) and OP (organic P) contents increased by 136% and 48% in the newly formed deposited layer (140 mm thick), respectively. The increased bioavailable P content significantly enhanced the capability of sediment solids to resupply labile P to porewater SRP. The stronger positive correlation between porewater soluble Fe (II) and SRP suggests that Fe redox cycling regulated internal P release. Our results suggest that dredging effectiveness will weaken over time due to the re-deposition of active P, which in turn increases the risk of sediment P release. To curb the release of sediment P, we recommend the implementation of additional in situ restoration techniques that improve the oxide layer of surface sediments and reduce sediment suspension.