Long-lasting, UV shielding, and cellulose-based avermectin nano/micro spheres with dual smart stimuli-microenvironment responsiveness for Plutella xylostella control.

The requirement to improve the efficiency of pesticide utilization has led to the development of sustainable and smart stimuli-responsive pesticide delivery systems. Herein, a novel avermectin nano/micro spheres (AVM@HPMC-Oxalate) with sensitive stimuli-response function target to the Lepidoptera pests midgut microenvironment (pH 8.0-9.5) was constructed using hydroxypropyl methylcellulose (HPMC) as the cost-effective and biodegradable material. The avermectin (AVM) loaded nano/micro sphere was achieved with high AVM loading capacity (up to 66.8 %). The simulated release experiment proved the rapid stimuli-responsive and pesticides release function in weak alkaline (pH 9) or cellulase environment, and the release kinetics were explained through release models and SEM characterization. Besides, the nano/micro sphere size made AVM@HPMC-Oxalate has higher foliar retention rate (1.6-2.1-fold higher than commercial formulation) which is beneficial for improving the utilization of pesticides. The in vivo bioassay proved that AVM@HPMC-Oxalate could achieve the long-term control of Plutella xylostella by extending UV shielding performance (9 fold higher than commercial formulation). After 3 h of irradiation, the mortality rate of P. xylostella treated by AVM@HPMC-Oxalate still up to 56.7 % ± 5.8 %. Moreover, AVM@HPMC-Oxalate was less toxic to non-target organisms, and the acute toxicity to zebrafish was reduced by 2-fold compared with AVM technical.

Multi-Stimuli-Responsive, Topology-Regulated, and Lignin-Based Nano/Microcapsules from Pickering Emulsion Templates for Bidirectional Delivery of Pesticides.

The increasing demand for improving pesticide utilization efficiency has prompted the development of sustainable, targeted, and stimuli-responsive delivery systems. Herein, a multi-stimuli-responsive nano/microcapsule bidirectional delivery system loaded with pyraclostrobin (Pyr) is prepared through interfacial cross-linking from a lignin-based Pickering emulsion template. During this process, methacrylated alkali lignin nanoparticles (LNPs) are utilized as stabilizers for the tunable oil-water (O/W) Pickering emulsion. Subsequently, a thiol-ene radical reaction occurs with the acid-labile cross-linkers at the oil-water interface, leading to the formation of lignin nano/microcapsules (LNCs) with various topological shapes. Through the investigation of the polymerization process and the structure of LNC, it was found that the amphiphilicity-driven diffusion and distribution of cyclohexanone impact the topology of LNC. The obtained Pyr@LNC exhibits high encapsulation efficiency, tunable size, and excellent UV shielding to Pyr. Additionally, the flexible topology of the Pyr@LNC shell enhances the retention and adhesion of the foliar surface. Furthermore, Pyr@LNC exhibits pH/laccase-responsive targeting against Botrytis disease, enabling the intelligent release of Pyr. The in vivo fungicidal activity shows that efficacy of Pyr@LNC is 53% ± 2% at 14 days postspraying, whereas the effectiveness of Pyr suspension concentrate is only 29% ± 4%, and the acute toxicity of Pyr@LNC to zebrafish is reduced by more than 9-fold compared with that of Pyr technical. Moreover, confocal laser scanning microscopy shows that the LNCs can be bidirectionally translocated in plants. Therefore, the topology-regulated bidirectional delivery system LNC has great practical potential for sustainable agriculture.

Phage-Based antibacterial hydrogels for bacterial targeting and Ablation: Progress and perspective.

The emergence of drug-resistant bacteria makes antibiotics inadequate to treat bacterial infections, which is now a global problem. Phage as a virus with specific recognition ability can effectively kill the bacteria, which is an efficacious antibacterial material to replace antibiotics. Phage-based hydrogels have good biocompatibility and antibacterial effect at the site of infection. Phage hydrogels have remarkable antibacterial effects on targeted bacteria because of their specific targeted bactericidal ability, but there are few reports and reviews on phage hydrogels. This paper discusses the construction method of phage-based antibacterial hydrogels (PAGs), summarizes the advantages related to PAGs and their applications in the direction of wound healing, treating bone bacterial infections, gastrointestinal infection treatment and other application, and finally gives an outlook on the development and research of PAGs.

Near-infrared-responsive CuS@Cu-MOF nanocomposite with high foliar retention and extended persistence for controlling strawberry anthracnose.

Strawberry anthracnose (Colletotrichum gloeosporioides) exhibits a high pathogenicity, capable of directly infecting leaves through natural openings, resulting in devastating impacts on strawberries. Here, nanocomposite (CuS@Cu-MOF) was prepared with a high photothermal conversion efficiency of 35.3% and a strong response to near-infrared light (NIR) by locally growing CuS nanoparticles on the surface of a copper-based metal-organic framework (Cu-MOF) through in situ sulfurization. The porosity of Cu-MOF facilitated efficient encapsulation of the pesticide difenoconazole within CuS@Cu-MOF (DIF/CuS@Cu-MOF), achieving a loading potential of 19.18 ± 1.07%. Under NIR light irradiation, DIF/CuS@Cu-MOF showed an explosive release of DIF, which was 2.7 times higher than that under dark conditions. DIF/CuS@Cu-MOF exhibited a 43.9% increase in efficacy against C. gloeosporioides compared to difenoconazole microemulsion (DIF ME), demonstrating prolonged effectiveness. The EC50 values for DIF and DIF/CuS@Cu-MOF were 0.219 and 0.189 µg/mL, respectively. Confocal laser scanning microscopy demonstrated that the fluorescently labeled CuS@Cu-MOF acted as a penetrative carrier for the uptake of hyphae. Furthermore, DIF/CuS@Cu-MOF exhibited more substantial resistance to rainwater wash-off than DIF ME, with retention levels on the surfaces of cucumber leaves (hydrophilicity) and peanut leaves (hydrophobicity) increasing by 36.5-fold and 9.4-fold, respectively. These findings underscore the potential of nanocomposite to enhance pesticide utilization efficiency and leaf retention.

Bidirectional Uptake, Transfer, and Transport of Dextran-Based Nanoparticles in Plants for Multidimensional Enhancement of Pesticide Utilization.

The development of effective multifunctional nano-delivery approaches for pesticide absorption remains a challenge. Here, a dextran-based pesticide delivery system (MBD) is constructed to deliver tebuconazole for multidimensionally enhancing its effective utilization on tomato plants. Spherical MBD nanoparticles are obtained through two-step esterification of dextran, followed by tebuconazole loading using the Michael addition reaction. Confocal laser scanning microscopy shows that fluorescein isothiocyanate-labeled MBD nanoparticles can be bidirectionally transported in tomato plants and a modified quick, easy, cheap, effective, rugged, and safe-HPLC approach demonstrates the capacity to carry tebuconazole to plant tissues after 24 h of root uptake and foliar spray, respectively. Additionally, MBD nanoparticles could increase the retention of tebuconazole on tomato leaves by up to nearly 2.1 times compared with the tebuconazole technical material by measuring the tebuconazole content retained on the leaves. In vitro antifungal and pot experiments show that MBD nanoparticles improve the inhibitory effect of tebuconazole against botrytis cinerea by 58.4% and the protection against tomato gray molds by 74.9% compared with commercial suspensions. Furthermore, the MBD nanoparticles do not affect the healthy growth of tomato plants. These results underline the potential for the delivery system to provide a strategy for multidimensional enhancement of pesticide efficacy.

SP-141 targets Trs85 to inhibit rice blast fungus infection and functions as a potential broad-spectrum antifungal agent.

Rice blast is a devastating disease worldwide, threatening rice production and food security. The blast fungus Magnaporthe oryzae invades the host via the appressorium, a specialized pressure-generating structure that generates enormous turgor pressure to penetrate the host cuticle. However, owing to ongoing evolution of fungicide resistance, it is vitally important to identify new targets and fungicides. Here, we show that Trs85, a subunit of the transport protein particle III complex, is essential for appressorium-mediated infection in M. oryzae. We explain how Trs85 regulates autophagy through Ypt1 (a small guanosine triphosphatase protein) in M. oryzae. We then identify a key conserved amphipathic α helix within Trs85 that is associated with pathogenicity of M. oryzae. Through computer-aided screening, we identify a lead compound, SP-141, that affects autophagy and the Trs85-Ypt1 interaction. SP-141 demonstrates a substantial capacity to effectively inhibit infection caused by the rice blast fungus while also exhibiting wide-ranging potential as an antifungal agent with broad-spectrum activity. Taken together, our data show that Trs85 is a potential new target and that SP-141 has potential for the control of rice blast. Our findings thus provide a novel strategy that may help in the fight against rice blast.

Tea polyphenols (TPP) as a promising wound healing agent: TPP exerts multiple and distinct mechanisms at different phases of wound healing in a mouse model.

Polyphenols have been widely used to treat various chronic skin diseases because they are beneficial in wound healing and show anti-inflammatory effects, however, the mechanism of action remains ambiguous. Previously, we reported the wound healing capability of tea polyphenols (TPP), the major functional component of tea, in vivo. The current study aimed to address the mechanisms of TPP in wound healing during different phases (inflammation, proliferation and remodeling). During the inflammation phase, TPP reduced the production of proinflammatory cytokines (IL-1ß, IL-6 and TNF-α) and inhibited infiltration of neutrophils; during the proliferation phase, TPP promoted the expression of growth factor VEGF-A, which can promote vascular endothelial cell division and induce angiogenesis; TPP improved the morphology of the wound and restored the ratio of type III/I collagens during the remodeling phase, as determined by Masson-trichrome staining and Sirius red staining assays. By tracking the changes in the wound area, TPP and recombinant human epidermal growth factor (rhEGF), rather than povidone-iodine (PVP-I), were able to promote wound healing. These results suggest that TPP plays a pivotal role in all the key stages of wound healing and displays distinct mechanisms from rhEGF, suggesting clinical significance for the future application of TPP as a natural wound healing agent.

Lignin-based non-crosslinked nanocarriers: A promising delivery system of pesticide for development of sustainable agriculture.

Lignin sulfonate (LS), a waste material from the paper pulping, was modified with benzoic anhydride to obtain benzoylated lignin sulfonates of adjustable hydrophilicity (BLS). When BLS was combined with difenoconazole (Di), a broad-spectrum fungicide, lignin-based, non-crosslinked nanoparticles were obtained either by solvent exchange or solvent evaporation. When a mass ratio of 1:5 LS: benzoic anhydride was used, the Di release from Di@BLS5 after 1248 h was ca. 74 %, while a commercial difenoconazole microemulsion (Di ME) reached 100 % already after 96 h, proving the sustained release from the lignin nanocarriers. The formulation of Di in lignin-based nanocarriers also improved the UV stability and the foliar retention of Di compared to the commercial formulation of the fungicide. Bioactivity assay showed that Di@BLS5 exhibited high activities and duration against strawberry anthracnose (Colletotrichum gloeosporioides). Overall, the construction of fungicide delivery nano-platform using BLS via a simple non-crosslinked approach is a novel and promising way to develop new formulations for nanopesticide and the development of sustainable agriculture.

Acetalated dextran microparticles for the smart delivery of pyraclostrobin to control Sclerotinia diseases.

Dextran has emerged as a promising biopolymer carrier for controlled release formulations of pesticides. In this study, pH-sensitive acetalated dextran microparticles (Pyr@Ac-Dex) are prepared to encapsulate and control the release of pyraclostrobin (Pyr). In vitro fungicidal activity experiments showed that the prepared Pyr@Ac-Dex particles show comparable fungicidal ability against S. sclerotiorum compared to that of Pyr technical. In a 10-day pot experiment, the control efficacy of the Pyr@Ac-Dex treatment against S. sclerotiorum (77.1%) is significantly higher than that of Pyr emulsifiable concentrate (Pyr EC) treatment (42.4%). Photodegradation experiments show that compared to Pyr technical, Pyr@Ac-Dex doubles the half-life of Pyr in water. Acute toxicity experiments show that Pyr@Ac-Dex significantly reduced the acute exposure toxicity of Pyr to zebrafish. This study provides an environmentally friendly, feasible, and sustainable strategy for plant disease management.

pH-Responsive On-Demand Alkaloids Release from Core-Shell ZnO@ZIF-8 Nanosphere for Synergistic Control of Bacterial Wilt Disease.

Developing an effective and safe technology to control severe bacterial diseases in agriculture has attracted significant attention. Here, ZnO nanosphere and ZIF-8 are employed as core and shell, respectively, and then a pH-responsive core-shell nanocarrier (ZnO-Z) was prepared by in situ crystal growth strategy. The bactericide berberine (Ber) was further loaded to form Ber-loaded ZnO-Z (Ber@ZnO-Z) for control of tomato bacterial wilt disease. Results demonstrated that Ber@ZnO-Z could release Ber rapidly in an acidic environment, which corresponded to the pH of the soil where the tomato bacterial wilt disease often outbreak. In vitro experiments showed that the antibacterial activity of Ber@ZnO-Z was about 4.5 times and 1.8 times higher than that of Ber and ZnO-Z, respectively. It was because Ber@ZnO-Z could induce ROS generation, resulting in DNA damage, cytoplasm leakage, and membrane permeability changes so the released Ber without penetrability more easily penetrated the bacteria to achieve an efficient synergistic bactericidal effect with ZnO-Z carriers after combining with DNA. Pot experiments also showed that Ber@ZnO-Z significantly reduced disease severity with a wilt index of 45.8% on day 14 after inoculation, compared to 94.4% for the commercial berberine aqueous solution. More importantly, ZnO-Z carriers did not accumulate in aboveground parts of plants and did not affect plant growth in a short period. This work provides guidance for the effective control of soil-borne bacterial diseases and the development of sustainable agriculture.

Reductions in Intestinal Taurine-Conjugated Bile Acids and Short-Chain Fatty Acid-Producing Bacteria Might be Novel Mechanisms of Type 2 Diabetes Mellitus in Otsuka Long-Evans Tokushima Fatty Rats.

BACKGROUND: The pathogenesis of spontaneously diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) rats, among the best models for human type 2 diabetes mellitus (T2DM), remains poorly defined. Therefore, we investigated the dynamic changes in taurine-conjugated bile acids (T-BAs) and intestinal microbiota during T2DM development in OLETF rats. METHODS: OLETF rats and corresponding diabetes-resistant Long Evans Tokushima Otsuka (LETO) rats were fed a normal baseline diet. The progress of T2DM was divided into four phases, including normal glycemia-normal insulinemia (baseline), normal glycemia-hyperinsulinemia, impaired glucose tolerance, and DM. Body weight, liver function, blood lipids, fasting plasma glucose, fasting plasma insulin, fasting plasma glucagon-like peptide (GLP)-1 and GLP-2, serum and fecal T-BAs, and gut microbiota were analyzed during the entire course of T2DM development. RESULTS: There were reductions in fecal T-BAs and short-chain fatty acids (SCFAs)-producing bacteria including Phascolarctobacterium and Lactobacillus in OLETF rats compared with those in LETO rats at baseline, and low levels of fecal T-BAs and SCFAs-producing bacteria were maintained throughout the whole course of the development of T2DM among OLETF rats compared with those in corresponding age-matched LETO rats. Fecal taurine-conjugated chenodeoxycholic acid correlated positively with Phascolarctobacterium. Fecal taurine-conjugated deoxycholic acid correlated positively with Lactobacillus and fasting plasma GLP-1 and inversely with fasting plasma glucose. CONCLUSION: The fecal BAs profiles and microbiota structure among OLETF rats were different from those of LETO rats during the entire course of T2DM development, indicating that reductions in intestinal T-BAs and specific SCFA-producing bacteria may be potential mechanisms of T2DM in OLETF rats.

Incidence, and preoperative and intraoperative prognostic factors of deep venous thrombosis in patients with glioma following craniotomy.

OBJECTIVES: The aim of this study was to investigate the incidence of deep vein thrombosis (DVT) and the preoperative and intraoperative risk factors associated with DVT in glioma patients METHODS: We conducted a retrospective analysis of data obtained from glioma patients at Sanbo Hospital (Beijing, China) between 2018 and 2021. Symptomatic DVT was confirmed by Doppler ultrasonography. Multivariable logistic regression analysis was used to identify preoperative and intraoperative characteristics associated with DVT. Basic clinical variables and laboratory results were analyzed. RESULTS: A total of 492 glioma patients were included. Of these, 73 (14.84%) developed DVT, and three (0.61%) developed DVT and pulmonary embolism (PE). Multivariate analyses revealed that the following factors were highly predictive of post-operative DVT: older age ranges of 46--55 years (odds ratio [OR]: 2.94; 95% confidence interval [CI]: 1.41--6.13; p = 0.004), 56--65 years (OR: 7.86; 95% CI: 3.63--17.03; p < 0.001), and > 65 years (OR: 4.94; 95% CI: 1.83--13.33; p = 0.002); partial thromboplastin time (APTT; OR: 0.91; 95% CI: 0.84--1.00; p = 0.040); D-dimer (OR: 2.21; 95% CI: 1.28--3.82; p = 0.005); and surgery duration (OR: 2.87; 95% CI: 1.6 --5.07; p < 0.001) CONCLUSIONS: Older age, preoperative APTT, D-dimer, and surgery duration independently increased the risk of developing postoperative DVT. These findings may facilitate the development of a thrombosis risk score that will allow physicians to develop individualized strategies to prevent DVT as early as possible.

A Light-Triggered pH-Responsive Metal-Organic Framework for Smart Delivery of Fungicide to Control Sclerotinia Diseases of Oilseed Rape.

Using a simple one-pot method, we developed a prochloraz (Pro) and pH-jump reagent-loaded zeolitic imidazolate framework-8 (PD@ZIF-8) composite for the smart control of Sclerotinia disease. The pH-jump reagent can induce the acidic degradation of ZIF-8 using UV light to realize the controlled release of Pro. Thus, the physical properties of PD@ZIF-8, such as its release, formulation stability, and adhesion, were investigated in detail. The results showed that the quantity of Pro released by PD@ZIF-8 under UV light irradiation (365 nm) was 63.4 ± 3.5%, whereas under dark conditions, it was only 13.7 ± 0.8%. In vitro activity indicated that the EC50 of PD@ZIF-8 under UV light irradiation was 0.122 ± 0.02 µg/mL, which was not significantly different from that of Pro (0.107 ± 0.01 µg/mL). Pot experiments showed that the efficacy of PD@ZIF-8 under light irradiation was 51.2 ± 5.7% for a fungal infection at 14 days post-spraying, whereas the effectiveness of prochloraz emulsion in water was only 9.3 ± 3.3%. Furthermore, fluorescence tracking of ZIF-8 and biosafety experiments showed that ZIF-8 could be absorbed by plant leaves and transported to various parts of oilseed rape in a short period of time and that PD@ZIF-8 was relatively safe for plants and HepG2 cells. These results highlight the potential of the composite to provide efficient and smart delivery of fungicides into plants for protection against diseases and provide an idea for developing sustainable agriculture.

A temperature-responsive release cellulose-based microcapsule loaded with chlorpyrifos for sustainable pest control.

Controlled pesticide release in response to environmental stimuli by encapsulating pesticide in carrier is a feasible approach to improve the effective utilization rate. Here, a temperature-responsive release microcapsule loaded with chlorpyrifos (CPF@CM) was prepared from n-hexadecane-in-water emulsions via interfacial polymerization. The microcapsule was consisted of nanofibrillated cellulose (NFC) as the shell wall material and isophorone diisocyanate (IPDI) as the crosslinker. The prepared CPF@CM had pesticide-loading efficiency (33.1 wt%) and favorable adhesion on the surface of cucumber and peanut foliage compared with conventional formulation. Additionally, CPF@CM could protect chlorpyrifos against photodegradation effectively. The in vitro release test showed that microcapsule had adjustable controlled-release characteristics with the change in temperature based on phase transition of the n-hexadecane core. Bioassay studies showed that control efficacy of CPF@CM microcapsule against P. xylostella was positively correlated with temperature because of temperature-induced changes in release rate. The acute toxicity of CPF@CM to zebrafish was reduced more than 5-fold compared with that of CPF technical. These results indicated that the microcapsule release system has great potential in the development of an effective and environmentally friendly pesticide formulation.

3D, eco-friendly metal-organic frameworks@carbon nanotube aerogels composite materials for removal of pesticides in water.

To alleviate the secondary risks of using metal-organic frameworks (MOFs) nanoparticles as adsorbent, a novel method of loading two MOFs (ZIF-8 or UiO66-NH2) on the carbon nanotube aerogels (MPCA) by in situ nucleation and growth of MOFs nanoparticles onto carbon nanotubes were developed. The prepared MOFs@MPCA aerogels were well characterized via SEM, TEM, EDS, FT-IR, XRD and XPS to reveal the microstructure and formation mechanism of MOF@MPCA. Besides, the hydrophilia, mechanical property and thermostability of MOF@MPCA were investigated. The results showed that MOF@MPCA had good hydrophilia, compression resilience and thermostability. The study on the ability to adsorb herbicides (chipton and alachlor) showed that the adsorption capacity of MOF@MPCA was stronger compared with single MOFs nanoparticles, which indicated that there was a synergistic effect between MOFs and MPCA. The equilibrium adsorption capacity of chipton adsorbed by UiO66-NH2@MPCA was 227.3 mg/g, and can be expediently reused for 5 cycles without a significant decrease in adsorption performance. Moreover, the results of biosafety experiments showed that MPCA can reduce the risk of MOFs nanoparticles leakage into the environment and accumulation in organisms. This work can provide a new research idea, which has potential applications to remove pollutants effectively and safely from the environment.

Peripheral Blood Occludin Level as a Biomarker for Perioperative Cerebral Edema in Patients with Brain Tumors.

OBJECTIVE: Cerebral edema is a common complication of brain tumors in the perioperative period. However, there is currently no reliable and convenient method to evaluate the extent of brain edema. The objective is to explore the effectiveness of serum occludin on predicting the extent of perioperative brain edema and outcome in patients with brain tumors. METHODS: This prospective study enrolled 55 patients with brain tumors and 24 healthy controls in Sanbo Brain Hospital from June 2019 through November 2019. Serum occludin levels were measured preoperatively and on postoperative day 1. Peritumoral edema was assessed preoperatively using MRI. Pericavity brain edema on postoperative day 1 was evaluated using CT. RESULTS: Compared with healthy controls, the serum occludin level was higher in patients with brain tumors both preoperatively and postoperatively (P < 0.001). The serum occludin level correlated positively with the degree of brain edema preoperatively (r = 0.78, P < 0.001) and postoperatively (r = 0.59, P < 0.001). At an optimal cutoff of 3.015 ng/mL, the preoperative serum occludin level discriminated between mild and severe preoperative brain edema with a sensitivity of 90.48% and specificity of 84.62%. At an optimal cutoff value of 3.033 ng/mL, the postoperative serum occludin level distinguished between mild and severe postoperative brain edema with a sensitivity of 97.30% and specificity of 55.56%. CONCLUSIONS: The serum occludin level is associated with cerebral edema and could potentially be used as a biomarker for perioperative cerebral edema. This trial is registered with ChiCTR1900023742.

Preoperative and intraoperative predictors of deep venous thrombosis in adult patients undergoing craniotomy for brain tumors: A Chinese single-center, retrospective study.

PURPOSE: Brain tumor resection by craniotomy is associated with a high risk of deep vein thrombosis (DVT). This study evaluated the incidence and preoperative and intraoperative risk factors for DVT within 30 days of surgery. METHODS: The analysis included: 1) basic clinical variables (patient age, sex, body mass index [BMI], tumor location, and tumor histology); 2) blood test results before operation, such as leukocytes, platelets, and coagulation parameters; and 3) surgical factors (total amount of blood lost, anesthesia mode, and surgery duration). RESULTS: Of the 1670 patients, 206 (12.34%) had DVT and nine (0.54%) had both DVT and pulmonary embolism (PE) after surgery. Preoperative and intraoperative factors independently associated with DVT/PE were: older age 46-55 years (odds ratio [OR]: 2.87; 95% confidence interval [CI]: 1.83-4.50; P < 0.001), age 56-65 years (OR: 5.24; 95% CI: 3.27-8.40; P < 0.001), age > 65 years (OR: 6.00; 95% CI: 3.45-10.45; P < 0.001), BMI (OR: 1.03; 95% CI: 1.00-1.05; P = 0.029), activated partial thromboplastin time [APTT] (OR: 0.91; 95% CI: 0.86-0.95; P = 0.000), D-dimer (OR: 1.69; 95% CI: 1.23-2.34; P = 0.001), high-grade glioma (OR: 2.09; 95% CI: 1.28-3.40; P = 0.003), glio-neuronal (OR: 3.30; 95% CI: 1.28-8.47; P = 0.013), craniopharyngioma (OR: 2.16; 95% CI: 1.13-4.10; P = 0.019), and surgery duration (OR: 1.82; 95% CI: 1.27-2.60; P = 0.001). CONCLUSIONS: Older age, BMI, preoperative APTT, D-dimer, tumor histology, and surgery duration independently increased the risk of developing postoperative DVT/PE. These findings provide prognostic information that will guide therapies aimed at minimizing the development of DVT/PE during hospitalization.

Origami spring-inspired metamaterials and robots: An attempt at fully programmable robotics.

Recent advances in three-dimensional printing technologies provide one way not only to speed up freeform fabrication but also to exert programmable control over mechanical properties. Besides, origami-inspired structures, origami-inspired metamaterials, and even origami-inspired robotics primarily demonstrate the promising potential for innovative inspirations of engineering solutions. The motivation of this work is to explore a fully programmable robotic perspective with a fusion of programmable metamaterials, programmable mechanics, and programmable fabrication. First, we proposed an illustrative roadmap for transforming an origami model into a fully programmable robotic system. Then, we introduced an origami spring model and revealed its shape-shifting geometry and intrinsic metamaterial mechanisms, especially the rarely switchable behavior from transverse compression to longitudinal stretchability, and the curvilinear deployment. Furthermore, we addressed the fabrication challenges of three-dimensional printable origami sheets considering three-dimensional printability, foldability with high elasticity, and good damage tolerance. Finally, we developed a fully soft manipulator in terms of the highly reversible compressibility of origami spring metamaterials. And we also devised a peristaltic crawling robot with undulatory movements induced by inclination deployment effect of origami spring metamaterials. Conceivably, the proposed fully programmable robotic system was demonstrated starting from programmable metamaterials, programmable mechanics, and programmable fabrication to programmable robotic behaviors. The contribution of this work also suggested that robotic morphing could be tunable by hierarchical programming from modeling and fabrication to actions.

High foliar affinity cellulose for the preparation of efficient and safe fipronil formulation.

In this work, fipronil was encapsulated within ethanediamine-modified carboxymethylcellulose (ACMC) to prepare an efficient and environmentally safe pesticide formulation (ACMCF). The chemical structure, morphology, foliar adhesion, bioactivity, and soil mobility of ACMCF were also systematically investigated. Results demonstrated that fipronil was encapsulated to form microcapsules successfully. Compared with the traditional fipronil emulsion (FE), ACMCF had a relatively high retention rate on cucumber and peanut leaves. The acute contact toxicity of ACMCF (LD50 = 0.151 µg a.i./bee) toward Apis mellifera was far lower than that of FE (LD50 = 0.00204 µg a.i./bee). Biological activity surveys confirmed that ACMCF has insecticidal ability against Plutella xylostella similar to that of FE. Moreover, the leaching and migration properties of ACMCF in three different kinds soils were weaker than those of FE. These results imply that ACMCF has promising application potential in increasing the effective utilization of fipronil and reducing risk to non-target organisms and the environment.

Innovative Approach to Nano Thiazole-Zn with Promising Physicochemical and Bioactive Properties by Nanoreactor Construction.

Nanotechnology has provided a novel approach for the preparation of a safe and highly effective pesticide formulation. Thiazole-Zn, a widely used bactericide, was successfully prepared at nanoscale by an innovative approach of final synthesis process control. Its plausible formation mechanism based on restricted particle aggregation in a nanoreactor was elucidated. Then in order to assess the application performance of thiazole-Zn nanoparticle, the nanoformulation (NPF) was conveniently formulated. Interestingly, the physicochemical properties of NPF showed better than that of the commercial pesticide formulation (CPF) in dispersibility, wettability, spreadability, and stability. At the same time, the in vitro bioassay showed that the minimum inhibitory concentrations (MICs) of NPF against Xanthomonas oryzae pv Oryzae (XOO), Xanthomonas oryzae pv Oryzicola (XOC), Erwinia carotovora subsp. Carotovora (Jones) Holland (ECC), and Erwinia chrysanthemi pv Zeae (ECZ) were 46.88, 93.75, 93.75, and 375.00 mg/L, respectively, whereas those of CPF were 93.75, 375.00, 375.00, and 875.00 mg/L, respectively. Therefore, NPF exhibited stronger antibacterial activity against the above-mentioned pathogens. Moreover, NPF was more effective to bacterial blight of rice than CPF in field trial. As a conclusion, nanotechnology for pesticides by synthesis process control will have a potential in improving the utilization efficiency and relieving the corresponding environmental pollution.