Pollen transfer efficiency in Erica depends on type of pollinator.

Pollen transfer efficiency (PTE; the proportion of pollen removed from flowers that reaches conspecific stigmas) is expected to vary with the type of pollinator and flower morphology, and to influence male siring success. Many species in the genus Erica are pollinated by bees (which consume pollen and should thus lower PTE) but during its radiation in the Cape, several independent shifts to both sunbird and long-proboscid fly (LP fly) pollinators, which do not consume pollen have taken place. Improvements in PTE could be one of the factors driving these pollinator shifts. PTE data for 15 Erica species (five for each of the three pollinator types) were collected and compared in relation to type of pollinator and anther exsertion. LP fly- and bird-pollinated species had higher PTE in comparison with bee-pollinated species. Species with inserted anthers had higher PTE than those with exserted anthers. This suggests that sunbirds and LP flies are more efficient pollinators than bees. Additionally, the study suggests that insertion of anthers within the corolla tube can reduce pollen losses.

Corolla stickiness prevents nectar robbing in Erica.

Floral stickiness is a rare trait with unknown function, but it is common in the mega-diverse Cape genus Erica (Ericaceae). This study investigated the role of stickiness measured as adhesive strength in Erica as protection against nectar robbing and its correlation with floral traits. We compared the incidence of nectar robbing in flowers of the same species with or without experimentally added stickiness, and amongst communities of co-occurring species with flowers differing in stickiness. Additionally, we tested the relationship between stickiness and pollination syndrome, corolla shape, corolla length and sepal-corolla ratio across the whole genus. Stickiness was correlated with lower floral damage rates within and between species, indicating it functions as an anti-nectar robbing trait. Across the genus Erica, stickiness is most strongly correlated with bird and long-proboscid fly pollination, presumably because of their larger nectar rewards. Stickiness was also correlated with floral traits that are often associated with high risk of being damaged by nectar robbers: narrow-mouthed corollas, long corollas and shorter sepals. These results show that corolla stickiness defends Erica flowers against nectar robbing and thereby potentially improves fitness.

Post-pollination barriers enable coexistence of pollinator-sharing ornithophilous Erica species.

Some evolutionary radiations produce a number of closely-related species that continue to coexist. In such plant systems, when pre-pollination barriers are weak, relatively strong post-pollination reproductive barriers are required to maintain species boundaries. Even when post-pollination barriers are in place, however, reproductive interference and pollinator dependence may strengthen selection for pre-pollination barriers. We assessed whether coexistence of species from the unusually speciose Erica genus in the fynbos biome, South Africa, is enabled through pre-pollination or post-pollination barriers. We also tested for reproductive interference and pollinator dependence. We investigated this in natural populations of three bird-pollinated Erica species (Erica plukenetii, E. curviflora and E. coccinea), which form part of a large guild of congeneric species that co-flower and share a single pollinator species (Orange-breasted Sunbird Anthobaphes violacea). At least two of the three pre-pollination barriers assessed (distribution ranges, flowering phenology and flower morphology) were weak in each species pair. Hand-pollination experiments revealed that seed set from heterospecific pollination (average 8%) was significantly lower than seed set from outcross pollination (average 50%), supporting the hypothesis that species boundaries are maintained through post-pollination barriers. Reproductive interference, assessed in one population by applying outcross pollen three hours after applying heterospecific pollen, significantly reduced seed set compared to outcross pollen alone. This may drive selection for traits that enhance pre-pollination barriers, particularly given that two of the three species were self-sterile, and therefore pollinator dependent. This study suggests that post-pollination reproductive barriers could facilitate the coexistence of congeneric species, in a recent radiation with weak pre-pollination reproductive barriers.