Metabolic Disrupting Effects of Polychlorinated Biphenyls Revealed by Long-Term Temporal Variations of Lipids in Detritivorous Fish from the Rio de la Plata Basin.

The long-term covariation (2002-2017) of lipids, polychlorinated biphenyls (PCBs), and sewage tracers was studied in the detritivorous fish (Prochilodus lineatus) and settling detritus from the Rio de la Plata. Fatty fish from polluted Buenos Aires area (BA) exhibited a significant decrease of muscle lipids (71 ± 12 to 29 ± 8.6% dry weight; p < 0.0001), triglycerides (94 to 85%, p < 0.001), and 18 carbon fatty acids (18C-FA: 59 ± 4.8 to 48 ± 1.4%; p < 0.01), reflecting a reduction of lipid accumulation, largely triglycerides enriched in 18C-FA, with a concomitant ∼20-times decline of PCBs (∼20 to >1 µg g-1 dw). The 2017 individuals of the BA series converged with leaner and more pristine northern fish (N), which showed no significant temporal variation (20 ± 10% lipids, 67 ± 8.7% triglycerides, 41 ± 8.1% 18C-FA, and 0.22 ± 0.42 µg g-1 dw PCB). In contrast, the fecal sterol tracer coprostanol remained abnormally higher in BA fish muscle with no significant temporal trend (120 ± 102 vs 6.6 ± 10 µg g-1 dw or 4.4 ± 2.8 vs 0.63 ± 1.2% sterols at N). The same pattern was observed in BA settling detritus, i.e., a temporal decrease of PCBs with high, stable coprostanol concentrations denoting sustained sewage inputs, while northern detritus was enriched in plant sterols. This long-term covariation of lipids and PCBs in fish muscle from polluted BA converging with more pristine and homogeneous northern specimens while maintaining a sewage-derived diet provides rare field evidence of the declining effect of PCBs controlling the temporal variation of muscular lipids in fish.

Alterations in the sterol signature of detritivorous fish along pollution gradients in the Río de la Plata basin (Argentina): From plant to sewage-based diet.

In order to assess the impact of sewage pollution on the diet of the strict detritivorous and migratory South American fish, Prochilodus lineatus, 16 sterol biomarkers were analyzed by gas chromatography-mass spectrometry from fish muscle (n: 144) collected along 1200 km in the Rio de la Plata basin. Sterol concentrations were fairly homogeneous (2.4 ± 1.3 mg g-1 dry weight), but their proportion in lipids was highly variable and inversely related to both body mass and lipid contents, reflecting the more conservative character of sterols compared to the rapid accumulation of fat as fish grows. As expected, the muscle sterol signature was widely dominated by cholesterol (92 ± 4.5% of total sterols), but it exhibited a remarkable diversity with variable proportions of fecal coprostanol (4.0 ± 4.4%) and plant sterols (3.1 ± 1.9%, e.g. sitosterol and campesterol). Muscle sterols exhibited contrasting geographical differences associated with dietary shifts from plant-derived detritus in the northern reaches of the basin (N: Paraná and Uruguay Rivers), to sewage dominated inputs at Buenos Aires (BA). Fish from BA are fattier (lipids: 35 ± 18 vs. 15 ± 9.0% at N), with higher total sterol contents (2.6 ± 1.3 vs. 1.9 ± 1.0 mg g-1), abundant coprostanol (5.3 ± 4.4 vs. 0.46 ± 1.1%) and lower plant sterols (2.6 ± 1.6 vs 4.6 ± 2.0%), reflecting a diet shifted to anthropogenic organic matter as opposed to vegetal detritus in the north. Accordingly, BA fish presented lower phyto/fecal sterol ratios (0.37 ± 0.21 vs. 0.91 ± 0.12 at N) and higher copro/epicoprostanol ratios (0.95 ± 0.082 vs 0.51 ± 0.25 at N), indicating fresh fecal inputs which provide a valuable supply of easily absorbed organic matter at this site. In addition, the sterol signature allowed to distinguish migratory fish from BA collected 900 km north (previously identified by their pollutant fingerprint and biochemical composition). In fact, coprostanol concentrations show a direct relationship with human populations along the basin, highlighting the usefulness of fecal sterol biomarkers as tracers of polluted fish stocks.

Long-Term (1970-2017) Temporal Trends of Polychlorinated Biphenyls in Fish, Settling Material, and Sediments from Populated and Remote Sites in Río de la Plata Estuary, Argentina.

Temporal trends of polychlorinated biphenyls (PCBs) were studied for detritivorous fish (1996-2017) and settling material (2002-2017) from polluted Buenos Aires coast and for a dated sediment core (1970-2013) from the outer Río de la Plata estuary. In spite of contrasting concentrations [5.3 ± 6.3 µg·g-1 dry weight (dw) for fish, 48 ± 26 ng·g-1 dw for settling material, and 1.5 ± 0.7 ng·g-1 dw for core], all three revealed exponentially decreasing trends over time (97%, 83%, and 83%, respectively). Time trends showed peak maxima coincident with Argentina's period of maximum PCB usage in 1973-1980 (80 cm depth in the core) and pulse discharges related to PCB banning in 2001-2002 (fish) with a lighter signature enriched in less persistent tri- and tetrachlorobiphenyls. The log-linear PCB time trends compare well with the predicted decrease for a high emission scenario from global emission data; the best fit was observed for the less impacted sediment core (-2%·year-1 versus -3%·year-1 for emission scenario). Steeper slopes are observed for the more polluted settling material (-5%·year-1) and especially for fish, in which the background decline trend tripled after the 2001 PCB pulse (from -7%·year-1 to -21%·year-1). These PCB time trends in related environmental compartments from contrasted sites provide rare evidence for evaluating the effectiveness of control measures in southern South America.

Situación laboral en España tras finalizar la residencia en Urología. Análisis de una encuesta a nivel nacional / Job status after the resident training period in Spain. Analysis of a national survey

Objetivos: Desde la instauración de las enseñanzas de especialización a través del sistema de residencia, la sanidad española ha pretendido mantener un equilibrio entre las necesidades establecidas y los profesionales formados, con el objetivo de evitar el déficit o exceso de especialistas sanitarios con las consecuencias que de ello pudieran derivar. El objetivo de la presente revisión es conocer la situación laboral de los médicos especialistas en urología al finalizar el periodo formativo MIR. Métodos: Se presentan los resultados de una encuesta destinada a médicos especialistas en urología que finalizaron su contrato de residencia desde el 2012 hasta 2016, valorando situación laboral, datos académicos y laborales durante los primeros meses tras la finalización de la formación especializada. Resultados: Se recogieron un total de 42 respuestas. La totalidad de los encuestados tuvo contrato laboral a los 6 meses tras finalizar el MIR. El 71% contaba con un contrato temporal, la mayoría con una duración menor de un año. Hay más números de contratos en la empresa pública, aunque aumentan progresivamente en la privada. Más de la mitad de los encuestados se encontraban satisfechos con su situación laboral. Conclusiones: La inserción laboral de los urólogos recientemente especializados es alta, llegando al 100% a los 6 meses de finalizar su especialización. No son tan positivas cuestiones relacionadas con la calidad laboral, observando una gran inestabilidad laboral asociada a una alta proporción de contratos temporales menores de 6 meses (AU)

Objectives: Since the establishment of specialization of medicine through the residency system, Spanish health care has sought to maintain a balance between established needs and trained professionals, with the aim of avoiding the deficit or excess of health specialists with its consequences. The objective of the present review is to know the working conditions of urologist specialists at the end of the residency training period. Méthods: The results of a survey for urologist who completed their residency contract from 2012 to 2016 are presented, assessing working status, academic and working data during the first months after the completion of specialized training. Results: A total of 42 surveys were collected. All respondents had a working contract within 6 months of completing their training. 71% had a temporary contract, most with duration of less than one year. There are more contract numbers in the public health system, although they increase progressively in the private sector. More than half of the respondents were satisfied with their work situation. Conclusiones: The work insertion of the recently specialized urologists is high, reaching 100% within 6 months of finishing their specialization. Labor quality issues are not so positive, observing great working instability associated to a high proportion of temporary contracts lower than 6 months (AU)

[Job status after the resident training period in Spain. Analysis of a national survey.] / Situación laboral en España tras finalizar la residencia en Urología. Análisis de una encuesta a nivel nacional.

OBJECTIVES: Since the establishment of specialization of medicine through the residency system, Spanish health care has sought to maintain a balance between established needs and trained professionals, with the aim of avoiding the deficit or excess of health specialists with its consequences. The objective of the present review is to know the working conditions of urologist specialists at the end of the residency training period. METHODS: The results of a survey for urologist who completed their residency contract from 2012 to 2016 are presented, assessing working status, academic and working data during the first months after the completion of specialized training. RESULTS: A total of 42 surveys were collected. All respondents had a working contract within 6 months of completing their training. 71% had a temporary contract, most with duration of less than one year. There are more contract numbers in the public health system, although they increase progressively in the private sector. More than half of the respondents were satisfied with their work situation. CONCLUSIONS: The work insertion of the recently specialized urologists is high, reaching 100% within 6 months of finishing their specialization. Labor quality issues are not so positive, observing great working instability associated to a high proportion of temporary contracts lower than 6 months.

Airborne PCB patterns and urban scale in the Southern Río de la Plata Basin, Argentina.

Atmospheric Polychlorinated Biphenyls (PCBs: ∑ 42 congeners) collected by polyurethane passive samplers (PAS-PUFs) in 29 stations from July 2010 to February 2014 (n=141) in the most productive and populated Southern Rio de la Plata area in Argentina were evaluated to assess concentration gradients, potential sources and compositional profiles related to different land use and urbanization. On a global scale, total airborne PCBs concentrations are low/very low (below detection limit to 937pgm-3) and show a significant potential correlation with urban scale increasing 2.5 times each 10 times increase of population reflecting the primary role of urbanization controlling PCB emissions. Compositional patterns evaluated by principal component analysis (PCA) of individual congeners indicated that highly populated atmospheres are enriched in lighter, more volatile tri, tetra and penta chlorine congeners of lighter Aroclor mixtures (from 1242 to 1254) suggesting actual emission of fresh PCBs signatures from sealants, combustion and/or electrical equipment. Sub urban and rural sites show a gradual transition to heavier Aroclor mixtures (from 1254 to 1260) with predominance of more persistent hexa and hepta PCBs indicating an aged background signal resulting from long range transport and/or re-emission from historic reservoirs such as soils.

Massive airborne Endosulfan inputs related to intensive agriculture in Argentina's Pampa.

In order to evaluate the impact of intensive agriculture on air quality in the most productive and populated Argentina's Pampas, a comprehensive assessment of airborne Endosulfan (∑Endo) was performed using polyurethane passive samplers deployed from 2010 to 2013 covering the critical period of Endosulfan restrictions at twenty nine sites in the Rural Pampa and Great Buenos Aires Metropolitan Area (GBA: Horticultural and Urban subareas). ∑Endo concentrations were very high and variable (0.01-63 ng m(-3)), exceeding worldwide reported maxima at Horticultural GBA and Rural Pampa with lowest values at Urban GBA (geometric means: 3.1, 1.1 and 0.53 ng m(-3), respectively). The composition was relatively fresh with strong predominance of Endo I (72 ± 18%) over Endo II (23 ± 15%) and Endo SO4 (5 ± 10%). Airborne ∑Endo was significantly correlated to annual soybean crop in Rural Pampa. ∑Endo concentrations showed a temporal pattern defined by consistent peaks enriched in Endo I during summer application periods, cold temperature minima with higher proportions of Endo SO4 and a general exponentially declining trend over time related to incipient control policies.