ABSTRACT
To explore the protective effect and mechanism of mild hypothermia on lung tissue damage after cardiopulmonary resuscitation in pigs. In this experiment, we electrically stimulated 16 pigs (30 ± 2 kg) for 10 min to cause ventricular fibrillation. The successfully resuscitated animals were randomly divided into two groups, a mild hypothermia group and a control group. We took arterial blood 0.5, 1, 3, and 6 h after ROSC recovery in the two groups of animals for blood gas analysis. We observed the structural changes of lung tissue under an electron microscope and calculate the wet weight/dry weight (W/D) ratio. We quantitatively analyzed the expression differences of representative inflammatory factors [interleukin-6 (IL-6) and tumor necrosis factor-alpha TNF-α)] through the ELISA test. We detected the expression levels of Bax, Bcl-2, and Caspase-3 proteins in lung tissues by Western blot. After 3 h and 6 h of spontaneous circulation was restored, compared with the control group, PaO2/FiO2 decreased significantly (P < 0.05). In addition, the pathological changes, lung W/D and lung MDA of the mild hypothermia group were better than those of the control group. The levels of IL-6 and TNF-α in the lung tissue of the mild hypothermia group were significantly lower than those of the control group (P < 0.05). The content of Caspase-3 and Bax in the mild hypothermia group was significantly lower than that of the control group. Our experiments have shown that mild hypothermia can reduce lung tissue damage after cardiopulmonary resuscitation.
Subject(s)
Cardiopulmonary Resuscitation , Hypothermia , Lung Injury , Humans , Animals , Swine , Lung Injury/etiology , Caspase 3 , Interleukin-6 , Tumor Necrosis Factor-alpha , bcl-2-Associated X ProteinABSTRACT
Tumor-associated macrophages (TAMs) are an important cause of tumorigenesis and tumor development. M2 macrophages can promote tumor growth while M1 macrophages kill tumor cells, therefore, polarizing macrophages to achieve a functional M1 phenotype could effectively play its anti-tumor role. In the current study, we synthesized a novel chrysin derivative which is termed as ChR-TD. And we found ChR-TD might be a ligand of TLR4 that polarized the TAMs towards M1 phenotype and played its anti-tumor role. Further study indicated that ChR-TD reprogrammed the macrophages into an M1 phenotype via TLR4 activation. Moreover, ChR-TD activated TLR4/NF-κB signaling pathway and promoted the NF-κB/p65 translocated into the nuclear, leading to the activation of NF-κB and proinflammatory cytokines release. In addition, type I interferon signaling was also activated by ChR-TD, leading to the expressions of IFN-α and IFN-ß and its targeted genes NOS2, MCP-1 and IP-10 were significantly increased in macrophages. Importantly, these effects were disturbed in TLR4-/- macrophages, which are constructed by using CRISPR/Cas9 system. And the molecule docking simulation further indicated that ChR-TD could bind to TLR4 and might be a ligand of TLR4. Hence, these findings suggested that ChR-TD might be a ligand of TLR4 and can be used as a potential lead compound for tumors treatment.
Subject(s)
Flavonoids/pharmacology , Macrophages/drug effects , Thiazoles/pharmacology , Toll-Like Receptor 4/metabolism , Animals , Cell Line , Cell Survival/drug effects , Female , Flavonoids/chemistry , Gene Expression Regulation/drug effects , Gene Knockdown Techniques , Humans , Interferon Type I/genetics , Interferon Type I/metabolism , Macrophages/physiology , Mice , Mice, Inbred BALB C , Models, Molecular , Molecular Structure , NF-kappa B/genetics , NF-kappa B/metabolism , Signal Transduction/drug effects , Structure-Activity Relationship , Thiazoles/chemistry , Toll-Like Receptor 4/geneticsABSTRACT
OBJECTIVE: This study is to evaluate the effects of the Adenoviral ßARKct (Adv. ßARKct) myocardial gene transfection following cardioplegic arrest on cardiopulmonary bypass (CPB) in a swine model. METHODS: Swine models of cardioplegic arrest on CPB were established after 5 days of myocardial injection of Adv. ßARKct or Adv. luciferase. The pigs were randomized into Adv. ßARKct, Control, and Sham groups. Invasive hemodynamics, cardiac function, biomarkers, and tissue morphology were assessed. RESULTS: Baseline data were similar among these groups. Hemodynamics and cardiac function showed a deteriorating trend throughout 6âh after weaning in ßARKct and Control groups. Compared with Control group, Adv. ßARKct treatment significantly elevated global and regional ventricular function (cardiac output, dp/dtmax, Ejection fraction, peak systolic longitudinal strain, and peak systolic strain rate) and altered hemodynamics (cardiac cycle efficiency and systemic vascular resistance). Moreover, inotropic score in ßARKct group was gradually decreased to 5.0â±â1.1, compared with Control group (6.2â±â0.9), at 6âh after weaning. Biomarkers in ßARKct group were significantly better than in Control group. Meanwhile, ßARKct treatment reduced the histopathologic injuries, rescued ß1-AR, SERCA2a, and RyR2 levels, and decreased the GRK2 levels in myocardial cells. CONCLUSION: Adv.ßARKct inhibits GRK2 and ameliorates myocardial injuries following cardioplegic arrest on CPB, via stabilizing ß1-AR, reducing mitochondrial damages and restoring sarcoplasmic reticulum Ca-handling protein expression.
Subject(s)
Cardiopulmonary Bypass , Genetic Therapy/methods , Adenoviridae/genetics , Animals , Hemodynamics/physiology , Random Allocation , SwineABSTRACT
OBJECTIVE: The aim of the study was to determine whether the inhibition of the G-protein-coupled receptor kinase 2 by adenoviral ßARKct cardiac gene transfer can ameliorate postresuscitation myocardial injury in pigs with cardiac arrest (CA) and explore the mechanism of myocardial protection. METHODS: Male landrace domestic pigs were randomized into the sham group (anesthetized and instrumented, but ventricular fibrillation was not induced) (nâ=â4), control group (ventricular fibrillation 8âmin, nâ=â8), and ßARKct group (ventricular fibrillation 8âmin, nâ=â8). Hemodynamic parameters were monitored continuously. Blood samples were collected at baseline, 30âmin, 2âh, 4âh, and 6âh after the return of spontaneous circulation (ROSC). Left ventricular ejection fraction was assessed by echocardiography at baseline and 6âh after ROSC. These animals were euthanized, and the cardiac tissue was removed for analysis at 6âh after ROSC. RESULTS: Compared with those in the sham group, left ventricular +dp/dtmax, -dp/dtmax, cardiac output (CO), and ejection fraction (EF) in the control group and the ßARKct group were significantly decreased at 6âh after the restoration of spontaneous circulation. However, the ßARKct treatment produced better left ventricular +dp/dtmax, -dp/dtmax, CO, and EF after ROSC. The ßARKct treatment also produced lower serum cardiac troponin I, CK-MB, and lactate after ROSC. Furthermore, the adenoviral ßARKct gene transfer significantly increased ß1 adrenergic receptors, SERCA2a, RyR2 levels, and decreased GRK2 levels compared to control. CONCLUSIONS: The inhibition of GRK2 by adenoviral ßARKct cardiac gene transfer can ameliorate postresuscitation myocardial injury through beneficial effects on restoring the sarcoplasmic reticulum Ca-handling proteins expression and upregulating the ß1-adrenergic receptor level after cardiac arrest.
Subject(s)
Adenoviridae , Cardiopulmonary Resuscitation , G-Protein-Coupled Receptor Kinase 2 , Heart Arrest , Heart Injuries , Transduction, Genetic , Animals , Disease Models, Animal , G-Protein-Coupled Receptor Kinase 2/biosynthesis , G-Protein-Coupled Receptor Kinase 2/genetics , Heart Arrest/genetics , Heart Arrest/metabolism , Heart Arrest/pathology , Heart Arrest/therapy , Heart Injuries/genetics , Heart Injuries/metabolism , Heart Injuries/pathology , Heart Injuries/therapy , Male , SwineABSTRACT
Background and Objective. It has been reported that sodium ferulate (SF) has hematopoietic function against anemia and immune regulation, inflammatory reaction inhibition, inhibition of tumor cell proliferation, cardiovascular and cerebrovascular protection, and other functions. Thus, this study aimed to investigate the effects of SF on angiotensin II- (AngII-) induced cardiac hypertrophy in mice through the MAPK/ERK and JNK signaling pathways. Methods. Seventy-two male C57BL/6J mice were selected and divided into 6 groups: control group, PBS group, model group (AngII), model + low-dose SF group (AngII + 10 mg/kg SF), model + high-dose SF group (AngII + 40 mg/kg SF), and model + high-dose SF + agonist group (AngII + 40 mg/kg SCU + 10 mg/kg TBHQ). After 7 d/14 d/28 days of treatments, the changes of blood pressure and heart rates of mice were compared. The morphology of myocardial tissue and the apoptosis rate of myocardial cells were observed. The mRNA and protein expressions of atrial natriuretic peptide (ANP), transforming growth factor-ß (TGF-ß), collagen III (Col III), and MAPK/ERK and JNK pathway-related proteins were detected after 28 days of treatments. Results. SF improved the mice's cardiac abnormality and decreased the apoptosis rate of myocardial cells in a time- and dose-dependent manner (all P < 0.05). MAPK/ERK pathway activator inhibited the protective effect of SF in myocardial tissue of mice (P < 0.05). SF could inhibit the expression of p-ERK, p-p38MAPK, and p-JNK and regulate the expressions of ANP, TGF-ß, and Col III (all P < 0.05). Conclusion. Our findings provide evidence that SF could protect against AngII-induced cardiac hypertrophy in mice by downregulating the MAPK/ERK and JNK pathways.
Subject(s)
Cardiomegaly/drug therapy , Cardiomegaly/enzymology , Cardiotonic Agents/therapeutic use , Coumaric Acids/therapeutic use , Extracellular Signal-Regulated MAP Kinases/metabolism , JNK Mitogen-Activated Protein Kinases/metabolism , MAP Kinase Signaling System , Angiotensin II , Animals , Apoptosis/drug effects , Blood Pressure/drug effects , Cardiomegaly/diagnostic imaging , Cardiomegaly/physiopathology , Cardiotonic Agents/pharmacology , Coumaric Acids/pharmacology , Diastole/drug effects , Enzyme Activation/drug effects , Heart Rate/drug effects , MAP Kinase Signaling System/drug effects , Male , Mice, Inbred C57BL , Myocardium/pathology , RNA, Messenger/genetics , RNA, Messenger/metabolism , Systole/drug effectsABSTRACT
Coronary heart disease (CHD) is a cardiovascular disease which is contributed by abnormal neovascularization. VEGFA (vascular endothelial growth factor A) and VEGFR2 (vascular endothelial growth factor receptor 2) have been revealed to be involved in the pathological angiogenesis. This study was intended to confirm whether single nucleotide polymorphisms (SNPs) of VEGFA and VEGFR2 were associated with CHD in a Chinese population, considering pathological features and living habits of CHD patients.Peripheral blood samples were collected from 810 CHD patients and 805 healthy individuals. Six tag SNPs within VEGFA and VEGFR2 were obtained from HapMap Database. Genotyping of SNPs was performed using SNapShot method (Applied Biosystems, Foster, CA). Odd ratios (ORs) and their 95% confidence intervals (95% CIs) were calculated to evaluate the association between SNPs and CHD risk.Under the allelic model, 6 SNPs of VEGFA and VEGFR2 were remarkably associated with the susceptibility to CHD. Genotype CT of rs3025039, TT of rs2305948, and AA of rs1873077 were associated with a reduced risk of CHD when smoking, alcohol intake and diabetes were considered, while homozygote GG of rs1570360 might elevate the susceptibility to CHD (all Pâ<â0.05) for patients who were addicted to smoking or those with hypertension. All of the combined effects of rs699947 (CC/CA) and rs2305948 (TT), rs3025039 (TT) and rs2305948 (TT), rs3025039 (CT) and rs1870377 (AA) had positive effects on the risk of CHD, respectively (all Pâ<â0.05). By contrast, the synthetic effects of rs69947 (CA/AA) and rs1870377 (TA), rs699947 (CA) and rs7667298 (GG), rs699947 (AA) and rs7667298 (GG), rs1570360 (GG) and rs2305948 (TT), as well as rs1570360 (GG) and rs1870377 (AA) all exhibited adverse effects on the risk of CHD, respectively (all Pâ<â0.05).Six polymorphisms in VEGFA and VEGFR2 may have substantial influence on the susceptibility to CHD in a Han Chinese population. Prospective cohort studies should be further designed to confirm the above conclusions.