Ecological risk assessment of pesticides in sediments of Pampean streams, Argentina.

After their application in agricultural areas, pesticides are dispersed throughout the environment, causing contamination problems. In Argentina, the main promoter of transgenic biotechnology in the region, the total consumption of agrochemicals has increased significantly in recent years. Most chemicals dumped near surface waters eventually end up in bottom sediments and can be toxic to the organisms that live there. However, published data on the mixing of pesticides in this compartment is still scarce. The objective of this work was to detect and quantify pesticide residues in the sediment of rural streams in the Pampas region and to carry out acute and chronic risk assessment in these aquatic ecosystems. The study area comprises the mountainous system of Tandilia, located in one of the most productive agricultural areas in the country. The concentration of atrazine, acetochlor, chlorpyrifos, cypermethrin, and 2,4-D in the sediment of four rural streams was determined in three different seasons, and the toxic units (TU) and the risk ratios (RQ) were calculated. All the compounds analyzed were detected in most of the sampling seasons and study sites, at concentrations higher than those established in the national and international quality guidelines for the protection of aquatic biota in surface waters and for human consumption. Chlorpyrifos, cypermethrin, and acetochlor were the main pesticides contributing to the TU and RQ values, representing a medium or high ecological risk in most of the sites. Therefore, the evaluation of these pesticides in the bottom sediments could be a decisive factor in assessing the risk to the aquatic environment.

Taxonomic and nontaxonomic responses to ecological changes in an urban lowland stream through the use of Chironomidae (Diptera) larvae.

Biotic descriptors--both taxonomic (diversity indices, species richness, and indicator species) and nontaxonomic (biomass, oxygen consumption/production, and anatomical deformities)--are useful tools for measuring a stream's ecological condition. Nontaxonomic parameters detect critical effects not reflected taxonomically. We analyzed changes in Chironomidae populations as taxonomic parameters and mentum deformities as a nontaxonomic parameter for evaluating a South-American-plains stream (Argentina). We performed samplings seasonally (March, June, September, and December; 2005) and physical and chemical measurements at three sampling sites of the stream (DC1 at river source, through DC3 downstream). The specimens collected in sediment and vegetation were analyzed to investigate mouth deformities in Chironomidae larvae. We identified a total of 9 taxa from Chironomidae and Orthocladiinae subfamilies. Shannon's diversity index for Chironomidae decreased from 1.6 bits ind⁻¹ (DC1) to 0.3 bits ind⁻¹ (DC3). The total density of the Chironomidae exhibited a great increase in abundance at site DC3, especially that of Chironomus calligraphus. Chironomidae taxonomic composition also changed among the three sites despite their spatial proximity: C. calligraphus, Goeldichironomus holoprasinus, Parachironomus longistilus, and Polypedilum were present at all three; Corynoneura and Paratanytarsu at DC1 only; Cricotopus at DC1 and DC3; Apedilum elachistus notably at DC2 and DC3; and Parametriocnemus only at DC2. C. calligraphus individuals from DC1 showed no mentum deformities; only 2 from DC2 exhibited mouth-structure alterations; while specimens from DC3 presented the most abnormalities, especially during autumn and late winter. Type-II deformities (supernumerary teeth and gaps) were the most common. Anatomical deformities are sublethal effects representing an early alert to chemically caused environmental degradation. Mentum deformities in benthic-Chironomidae larvae constitute an effective biological-surveillance tool for detecting adverse conditions in sediments and evaluating sediment-quality-criteria compliance. Taxonomic (community composition) and nontaxonomic (condition of larval mouth parts) descriptors, used together, can indicate a stream's ecological state.

Effects of discharges from the textile industry on the biotic integrity of benthic assemblages.

We explored the effects of a textile industry effluent on water quality, habitat quality and structural and functional responses of benthic communities in a lowland stream. Two sampling sites were selected: site 1 was located 300 m upstream of the outflow from the textile factory and site 2 was 500 m downstream from the discharge point. Samples of water, microbenthos, invertebrates and aquatic plants were taken seasonally. The effluent from the textile industry modified the structure of the microbenthic assemblages downstream, increased the density of organisms and the biomass of primary producers, but diminished the species richness. The oxygen consumption of the microbenthic community was 3 x higher downstream of the effluent and abnormal frustules of diatoms were noticed. The richness and abundance of invertebrate taxa were lower at the impacted site. The invertebrate modes of existence and the functional feeding groups were also significantly affected. This study is an important baseline for assessment of lowland streams with high water residence time and a notable development of hydrophytes. It will also provide a baseline for the monitoring and restoration, or remediation, programs using the metrics of biotic integrity, particularly in South American countries where such metrics are rarely employed.