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J Neurosci Methods ; 212(1): 100-5, 2013 Jan 15.
Article in English | MEDLINE | ID: mdl-23036661

ABSTRACT

There is an increasing need for a pig model for use in functional brain studies, but a system for determining precise stereotactic coordinates has yet to be developed. Thus, we devised a frameless navigation system for stereotactic positioning, and measured coordinates for the rostral region and the primary somatosensory cortex in the pig brain. Raw coordinates for somatic evoked potential recordings were obtained by passive optical tracking. The location was registered to a computed tomographic image in reference to four stable skull landmarks: the upper margin of each auditory meatus, the external occipital protuberance, and the point where the interfrontal suture crosses a line drawn between the two supraorbital foramina ("IF" point). The cortical position with the greatest response in evoked potential was mapped -51.0 ± 4.67 mm rostro-caudally, 9.1 ± 1.19 mm medio-laterally, and -8.8 ± 0.48 mm dorso-ventrally (means ± SD; n=3) to the IF point. These results show that frameless registration is useful for coordinate-based evoked-potential mapping of the rostral region of the Mexican hairless pig.


Subject(s)
Brain Mapping , Brain/physiology , Evoked Potentials/physiology , Neuronavigation/methods , Animals , Brain/anatomy & histology , Electric Stimulation/instrumentation , Electric Stimulation/methods , Electroencephalography , Female , Imaging, Three-Dimensional , Male , Neuronavigation/instrumentation , Stereotaxic Techniques , Swine , Tomography Scanners, X-Ray Computed
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