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1.
Rev. bras. pesqui. méd. biol ; Braz. j. med. biol. res;44(7): 618-623, July 2011. ilus
Article in English | LILACS | ID: lil-595709

ABSTRACT

Taurine has positive effects on bone metabolism. However, the effects of taurine on osteoblast apoptosis in vitro have not been reported. The aim of this study was to investigate the activity of taurine on apoptosis of mouse osteoblastic MC3T3-E1 cells. The data showed that 1, 5, 10, or 20 mM taurine resulted in 16.7, 34.2, 66.9, or 63.75 percent reduction of MC3T3-E1 cell apoptosis induced by the serum deprivation (serum-free α-MEM), respectively. Taurine (1, 5, or 10 mM) also reduced cytochrome c release and inhibited activation of caspase-3 and -9, which were measured using fluorogenic substrates for caspase-3/caspase-9, in serum-deprived MC3T3-E1 cells. Furthermore, taurine (10 mM) induced extracellular signal-regulated kinase (ERK) phosphorylation in MC3T3-E1 cells. Knockdown of the taurine transporter (TAUT) or treatment with the ERK-specific inhibitor PD98059 (10 μM) blocked the activation of ERK induced by taurine (10 mM) and abolished the anti-apoptotic effect of taurine (10 mM) in MC3T3-E1 cells. The present results demonstrate for the first time that taurine inhibits serum deprivation-induced osteoblast apoptosis via the TAUT/ERK signaling pathway.


Subject(s)
Animals , Cattle , Mice , Apoptosis/drug effects , Apoptosis/physiology , Extracellular Signal-Regulated MAP Kinases/metabolism , Membrane Glycoproteins/metabolism , Membrane Transport Proteins/metabolism , Osteoblasts/drug effects , Taurine/pharmacology , Analysis of Variance , Caspase 9/metabolism , /metabolism , Osteoblasts/metabolism , RNA, Messenger/metabolism
2.
Braz J Med Biol Res ; 44(7): 618-23, 2011 Jul.
Article in English | MEDLINE | ID: mdl-21710101

ABSTRACT

Taurine has positive effects on bone metabolism. However, the effects of taurine on osteoblast apoptosis in vitro have not been reported. The aim of this study was to investigate the activity of taurine on apoptosis of mouse osteoblastic MC3T3-E1 cells. The data showed that 1, 5, 10, or 20 mM taurine resulted in 16.7, 34.2, 66.9, or 63.75% reduction of MC3T3-E1 cell apoptosis induced by the serum deprivation (serum-free α-MEM), respectively. Taurine (1, 5, or 10 mM) also reduced cytochrome c release and inhibited activation of caspase-3 and -9, which were measured using fluorogenic substrates for caspase-3/caspase-9, in serum-deprived MC3T3-E1 cells. Furthermore, taurine (10 mM) induced extracellular signal-regulated kinase (ERK) phosphorylation in MC3T3-E1 cells. Knockdown of the taurine transporter (TAUT) or treatment with the ERK-specific inhibitor PD98059 (10 µM) blocked the activation of ERK induced by taurine (10 mM) and abolished the anti-apoptotic effect of taurine (10 mM) in MC3T3-E1 cells. The present results demonstrate for the first time that taurine inhibits serum deprivation-induced osteoblast apoptosis via the TAUT/ERK signaling pathway.


Subject(s)
Apoptosis/drug effects , Apoptosis/physiology , Extracellular Signal-Regulated MAP Kinases/metabolism , Membrane Glycoproteins/metabolism , Membrane Transport Proteins/metabolism , Osteoblasts/drug effects , Taurine/pharmacology , 3T3 Cells , Analysis of Variance , Animals , Caspase 3/metabolism , Caspase 9/metabolism , Cattle , Mice , Osteoblasts/metabolism , RNA, Messenger/metabolism
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