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1.
Cancer Lett ; : 217004, 2024 Jun 03.
Article En | MEDLINE | ID: mdl-38838765

Long non-coding RNA (lncRNA) is closely related to a variety of human cancers, which may provide huge potential biomarkers for cancer diagnosis and treatment. However, the aberrant expression of most lncRNAs in colorectal cancer (CRC) remains elusive. This study aims to explore the clinical significance and potential mechanism of lncRNA ABHD11 antisense RNA 1 (ABHD11-AS1) in the colorectal cancer. Here, we demonstrated that lncRNA ABHD11-AS1 is high-expressed in colorectal cancer (CRC) patients, and strongly related with poor prognosis. Functionally, ABHD11-AS1 suppresses ferroptosis and promotes proliferation and migration in CRC both in vitro and in vivo. Mechanically, lncRNA ABHD11-AS1 interacted with insulin-like growing factor 2 mRNA-binding protein 2 (IGF2BP2) to enhance FOXM1 stability, forming an ABHD11-AS1/FOXM1 positive feedback loop. E3 ligase tripartite motif containing 21 (TRIM21) promotes the degradation of IGF2BP2 via the K48-ubiquitin-lysosome pathway and ABHD11-AS1 promotes the interaction between IGF2BP2 and TRIM21 as scaffold platform. Furthermore, N6 -adenosine-methyltransferase-like 3 (METTL3) upregulated the stabilization of ABHD11-AS1 through the m6A reader IGF2BP2. Our study highlights ABHD11-AS1 as a significant regulator in CRC and it may become a potential target in future CRC treatment.

2.
Article En | MEDLINE | ID: mdl-38829703

BACKGROUND: Preclinical models of disease have suggested that targeting microRNA-21 may slow the decline in kidney function in individuals with Alport syndrome. The objective of this study was to investigate the effects of the anti-microRNA-21 oligonucleotide, lademirsen, on rate of estimated glomerular filtration rate (eGFR) decline in adults with Alport syndrome at risk of rapid disease progression. METHODS: This study was a phase 2 trial of lademirsen, with a randomized, double-blind, placebo-controlled period followed by an open-label period. Adults with Alport syndrome, eGFR >35 to <90 mL/min/1.73 m2, and evidence of rapidly progressive kidney dysfunction were randomized 2:1 to lademirsen 110 mg subcutaneously once weekly or placebo for 48 weeks. Following a planned interim analysis (after 24 of 43 randomized participants completed the Week 48 study visit or discontinued prior to Week 48), the trial was terminated for futility. RESULTS: Forty-three adults with Alport syndrome (26 men, 17 women) participated (mean age 34 years) and 28 (lademirsen: n=19; placebo: n=9) completed 48 weeks of double-blind treatment. All participants in both groups developed treatment-emergent adverse events (TEAEs), mainly respiratory tract infections, headache, dizziness, metabolic/electrolyte disturbances, and anemia. Treatment was discontinued in three lademirsen-treated participants in the double-blind period, and one participant in the open-label period, owing to TEAEs. The least-squares mean eGFR slope (95% confidence interval) over 48 weeks in the lademirsen and placebo groups was -5 (-8.7, -1.1) and -5 (-10.2, 0.8) mL/min/1.73 m2/year, respectively. No significant differences between groups were identified in eGFR at any timepoint or in proportion of participants with prespecified reductions in eGFR at Weeks 24 or 48. CONCLUSION: While anti-microRNA-21 therapy with lademirsen was generally well-tolerated with an acceptable safety profile, no meaningful improvement in rate of kidney function decline in adults with Alport syndrome at risk of rapidly progressive disease was observed.

3.
Fa Yi Xue Za Zhi ; 40(2): 128-134, 2024 Apr 25.
Article En, Zh | MEDLINE | ID: mdl-38847026

OBJECTIVES: To establish age estimation models of northern Chinese Han adults using cranial suture images obtained by CT and multiplanar reformation (MPR), and to explore the applicability of cranial suture closure rule in age estimation of northern Chinese Han population. METHODS: The head CT samples of 132 northern Chinese Han adults aged 29-80 years were retrospectively collected. Volume reconstruction (VR) and MPR were performed on the skull, and 160 cranial suture tomography images were generated for each sample. Then the MPR images of cranial sutures were scored according to the closure grading criteria, and the mean closure grades of sagittal suture, coronal sutures (both left and right) and lambdoid sutures (both left and right) were calculated respectively. Finally taking the above grades as independent variables, the linear regression model and four machine learning models for age estimation (gradient boosting regression, support vector regression, decision tree regression and Bayesian ridge regression) were established for northern Chinese Han adults age estimation. The accuracy of each model was evaluated. RESULTS: Each cranial suture closure grade was positively correlated with age and the correlation of sagittal suture was the highest. All four machine learning models had higher age estimation accuracy than linear regression model. The support vector regression model had the highest accuracy among the machine learning models with a mean absolute error of 9.542 years. CONCLUSIONS: The combination of skull CT-MPR and machine learning model can be used for age estimation in northern Chinese Han adults, but it is still necessary to combine with other adult age estimation indicators in forensic practice.


Age Determination by Skeleton , Asian People , Cranial Sutures , Machine Learning , Tomography, X-Ray Computed , Humans , Cranial Sutures/diagnostic imaging , Middle Aged , Adult , Aged , Aged, 80 and over , Age Determination by Skeleton/methods , Retrospective Studies , Female , China/ethnology , Male , Skull/diagnostic imaging , Forensic Anthropology/methods , Bayes Theorem , Image Processing, Computer-Assisted/methods , Imaging, Three-Dimensional , Ethnicity , Linear Models , East Asian People
4.
Antimicrob Agents Chemother ; : e0012424, 2024 May 01.
Article En | MEDLINE | ID: mdl-38690893

As an obligate aerobe, Mycobacterium tuberculosis relies on its branched electron transport chain (ETC) for energy production through oxidative phosphorylation. Regimens targeting ETC exhibit promising potential to enhance bactericidal activity against M. tuberculosis and hold the prospect of shortening treatment duration. Our previous research demonstrated that the bacteriostatic drug candidate TB47 (T) inhibited the growth of M. tuberculosis by targeting the cytochrome bc1 complex and exhibited synergistic activity with clofazimine (C). Here, we found synergistic activities between C and sudapyridine (S), a structural analog of bedaquiline (B). S has shown similar anti-tuberculosis efficacy and may share a mechanism of action with B, which inhibits ATP synthesis and the energy metabolism of bacteria. We evaluated the efficacy of SCT in combination with linezolid (L) or pyrazinamide (Z) using a well-established murine model of tuberculosis. Compared to the BPa(pretomanid)L regimen, SCT and SCTL demonstrated similar bactericidal and sterilizing activities. There was no significant difference in activity between SCT and SCTL. In contrast, SCZ and SCTZ showed much higher activities, with none of the 15 mice experiencing relapse after 2 months of treatment with either SCZ or SCTZ. However, T did not contribute to the activity of the SCZ. Our findings emphasize the efficacy and the potential clinical significance of combination therapy with ETC inhibitors. Additionally, cross-resistance exists not only between S and B but also between S/B and C. This is supported by our findings, as spontaneous S-resistant mutants exhibited mutations in Rv0678, which are associated with cross-resistance to B and C.

5.
ChemSusChem ; : e202400608, 2024 May 15.
Article En | MEDLINE | ID: mdl-38747335

A Cu/Co tandem catalysis protocol was developed to conduct the hydroformylation of olefins using CO2/H2 and PMHS (polymethylhydrosiloxane) as a readily available and environmentally friendly hydride source. This methodology was performed via a two-step approach consisting of the copper-catalyzed reduction of CO2 by hydrosilane and subsequent cobalt-promoted hydroformylation with H2 and the in situ formed CO. The optimized triphos oxide ligand, which presumably facilitates the migratory insertion of CO gives moderate to excellent yields for both terminal and internal alkenes. This earth-abundant metal catalysis provides a reliable and efficient way to afford useful aldehydes in industry using silicon by-product PMHS as hydrogen source and renewable CO2 as carbonyl source.

6.
Clin Interv Aging ; 19: 727-736, 2024.
Article En | MEDLINE | ID: mdl-38736560

Purpose: Construct an exercise intervention program for patients with sarcopenic obesity. Material and Methods: Based on the COM-B theoretical model and evidence-based principles, the program was constructed using qualitative methods of literature analysis and Delphi method. The Delphi panel consisted of 15 experts from the fields of clinical medicine, rehabilitation medicine, medical technology, and nursing. Results: Fifteen experts were consulted, and the consultation recovery rate was 100%; the authority coefficient of the 1st round was 0.83, with coefficients of variation ranging from 0.00 to 0.27, and importance scores ranging from (4.13±1.13) to (5±0); the authority coefficient of the 2nd round was 0.82, with coefficients of variation ranging from 0.00 to 0.20, and importance scores ranging from (4.53±0.64) to (5±0); Kendall's harmony coefficient was 0.102, 0.115, respectively, and the differences were statistically significant(P < 0.05). The constructed exercise intervention program for patients with sarcopenic obesity included 4 primary indicators, 12 secondary indicators, and 28 tertiary indicators. Conclusion: The constructed exercise intervention program for patients with sarcopenic obesity is scientific, feasible and generalizable, and can provide useful reference for related personnel to develop exercise programs for patients with sarcopenic obesity.


Delphi Technique , Exercise Therapy , Obesity , Sarcopenia , Humans , Obesity/therapy , Exercise Therapy/methods , Sarcopenia/rehabilitation , Male , Female , Middle Aged , Aged , Adult
7.
Sci Total Environ ; 932: 173033, 2024 Jul 01.
Article En | MEDLINE | ID: mdl-38723954

Microplastics (MPs) pollution has emerged as a global concern, and wastewater treatment plants (WWTPs) are one of the potential sources of MPs in the environment. However, the effect of polyethylene MPs (PE) on nitrogen (N) removal in moving bed biofilm reactor (MBBR) remains unclear. We hypothesized that PE would affect N removal in MBBR by influencing its microbial community. In this study, we investigated the impacts of different PE concentrations (100, 500, and 1000 µg/L) on N removal, enzyme activities, and microbial community in MBBR. Folin-phenol and anthrone colorimetric methods, oxidative stress and enzyme activity tests, and high-throughput sequencing combined with bioinformation analysis were used to decipher the potential mechanisms. The results demonstrated that 1000 µg/L PE had the greatest effect on NH4+-N and TN removal, with a decrease of 33.5 % and 35.2 %, and nitrifying and denitrifying enzyme activities were restrained by 29.5-39.6 % and 24.6-47.4 %. Polysaccharide and protein contents were enhanced by PE, except for 1000 µg/L PE, which decreased protein content by 65.4 mg/g VSS. The positive links of species interactions under 1000 µg/L PE exposure was 52.07 %, higher than under 500 µg/L (51.05 %) and 100 µg/L PE (50.35 %). Relative abundance of some metabolism pathways like carbohydrate metabolism and energy metabolism were restrained by 0.07-0.11 % and 0.27-0.4 %. Moreover, the total abundance of nitrification and denitrification genes both decreased under PE exposure. Overall, PE reduced N removal by affecting microbial community structure and species interactions, inhibiting some key metabolic pathways, and suppressing key enzyme activity and functional gene abundance. This paper provides new insights into assessing the risk of MPs to WWTPs, contributing to ensuring the health of aquatic ecosystems.


Biofilms , Bioreactors , Microbiota , Nitrogen , Polyethylene , Waste Disposal, Fluid , Water Pollutants, Chemical , Nitrogen/metabolism , Bioreactors/microbiology , Water Pollutants, Chemical/analysis , Waste Disposal, Fluid/methods , Microbiota/drug effects , Microplastics , Wastewater/chemistry
8.
Article En | MEDLINE | ID: mdl-38782719

BACKGROUND: Although congenital abnormalities of the kidney and urinary tract (CAKUT) is the leading cause of childhood onset chronic kidney disease (CKD) and kidney failure, comprehensive information on the disease burden among children and adolescents globally is lacking. We aim to report the trends and socioeconomic inequality of CAKUT burden for people aged 0-24 years from 1990 to 2019·. METHODS: We reported the prevalence, mortality and disability-adjusted life-years (DALYs) for CAKUT based on the Global Burden of Diseases, Injuries, and Risk Factors Study (GBD) 2019, quantified the association of disease burden and socio-demographic index (SDI), calculated the slope index of inequality (SII) the relative index of inequality (RII) and concentration index. RESULTS: In 2019, the global prevalence, mortality, and DALYs of CAKUT among individuals aged 0-24 years were 167.11 (95%Confident Interval 166.97, 167.25), 0.30 (0.29, 0.30), and 32.22 (32.16, 32.29) per 100 000 population. The greatest prevalence, mortality and DALYs were recorded in the 0-4 year age group. The greatest mortality and DALYs were recorded in low SDI countries and territories. During 1990 to 2019, the prevalence, mortality and DALYs decreased globally, while in low and low-middle countries and territories the reduction was much less slower. India, Nigeria and Pakistan had the highest DALYs. Saudi Arabia and China exhibited a markedly decrease of CAKUT burden. Globally for every 0.1 increase in SDI, there was a 20.53% reduction in mortality, a 16.31% decrease in DALYs, but a 0.38% rise in prevalence. CONCLUSIONS: Inequality for disease burden of varying SDI was increasing globally. Thus, specific preventive and health service measures are needed to reduce the global burden from CAKUT.

9.
Proc Natl Acad Sci U S A ; 121(23): e2400159121, 2024 Jun 04.
Article En | MEDLINE | ID: mdl-38814870

Lithium is an emerging strategic resource for modern energy transformation toward electrification and decarbonization. However, current mainstream direct lithium extraction technology via adsorption suffers from sluggish kinetics and intensive water usage, especially in arid/semiarid and cold salt-lake regions (natural land brines). Herein, an efficient proof-of-concept integrated solar microevaporator system is developed to realize synergetic solar-enhanced lithium recovery and water footprint management from hypersaline salt-lake brines. The 98% solar energy harvesting efficiency of the solar microevaporator system, elevating its local temperature, greatly promotes the endothermic Li+ extraction process and solar steam generation. Benefiting from the photothermal effect, enhanced water flux, and enriched local Li+ supply in nanoconfined space, a double-enhanced Li+ recovery capacity was delivered (increase from 12.4 to 28.7 mg g-1) under one sun, and adsorption kinetics rate (saturated within 6 h) also reached twice of that at 280 K (salt-lake temperature). Additionally, the self-assembly rotation feature endows the microevaporator system with distinct self-cleaning desalination ability, achieving near 100% water recovery from hypersaline brines for further self-sufficient Li+ elution. Outdoor comprehensive solar-powered experiment verified the feasibility of basically stable lithium recovery ability (>8 mg g-1) directly from natural hypersaline salt-lake brines with self-sustaining water recycling for Li+ elution (440 m3 water recovery per ton Li2CO3). This work offers an integrated solution for sustainable lithium recovery with near zero water/carbon consumption toward carbon neutrality.

10.
Int J Biol Macromol ; 271(Pt 2): 132566, 2024 May 23.
Article En | MEDLINE | ID: mdl-38795883

Nowadays, the development of sustainable molecularly imprinted polymers (MIPs) with high selectivity is still challenging due to the limitations of bio-based functional monomers. In this study, the highly selective and porous MIPs (LC-TMIPs) were designed and prepared on short amylose (SAM) as bio-based functional monomers, λ-cyhalothrin (LC) as a template molecule, and tetrafluoroterephthalonitrile as a rigid crosslinking agent. Static, dynamic, and selective adsorption experiments were conducted to investigate the adsorption performance. The results indicated that, compared to MIPs prepared using epichlorohydrin as flexible crosslinking agents, LC-TMIPs exhibited higher imprinting factor (3.93), selectivity (5.78), and adsorption capacity (35.79 mg g-1), as well as faster adsorption/desorption kinetics. The LC-TMIPs were used as sorbents for the selective determination of LC in both apple and cucumber samples by high-performance liquid chromatography. Under the optimal extraction conditions, the recoveries of the method reached 92.1-106.1 %, with a linear range of 1.5-30 ng g-1 and a detection limit of 0.5 ng g-1. The proposed preparation method of LC-TMIPs is expected to open a new way to prepare highly selective and sustainable MIPs for hydrophobic compounds.

11.
Sci Adv ; 10(22): eadj3760, 2024 May 31.
Article En | MEDLINE | ID: mdl-38820164

Intrinsic water evaporation demands a high energy input, which limits the efficacy of conventional interfacial solar evaporators. Here, we propose a nanoconfinement strategy altering inherent properties of water for solar-driven water evaporation using a highly uniform composite of vertically aligned Janus carbon nanotubes (CNTs). The water evaporation from the CNT shows the unexpected diameter-dependent evaporation rate, increasing abnormally with decreasing nanochannel diameter. The evaporation rate of CNT10@AAO evaporator thermodynamically exceeds the theoretical limit (1.47 kg m-2 hour-1 under one sun). A hybrid experimental, theoretical, and molecular simulation approach provided fundamental evidence of different nanoconfined water properties. The decreased number of H-bonds and lower interaction energy barrier of water molecules within CNT and formed water clusters may be one of the reasons for the less evaporative energy activating rapid nanoconfined water vaporization.

12.
Comput Methods Programs Biomed ; 251: 108212, 2024 Jun.
Article En | MEDLINE | ID: mdl-38754327

BACKGROUND AND OBJECTIVE: There is a rising interest in exploiting aggregate information from external medical studies to enhance the statistical analysis of a modestly sized internal dataset. Currently available software packages for analyzing survival data with a cure fraction ignore the potentially available auxiliary information. This paper aims at filling this gap by developing a new R package CureAuxSP that can include subgroup survival probabilities extracted outside into an interested internal survival dataset. METHODS: The newly developed R package CureAuxSP provides an efficient approach for information synthesis under the mixture cure models, including Cox proportional hazards mixture cure model and the accelerated failure time mixture cure model as special cases. It focuses on synthesizing subgroup survival probabilities at multiple time points and the underlying method development lies in the control variate technique. Evaluation of homogeneity assumption based on a test statistic can be automatically carried out by our package and if heterogeneity does exist, the original outputs can be further refined adaptively. RESULTS: The R package CureAuxSP provides a main function SMC.AxuSP() that helps us adaptively incorporate external subgroup survival probabilities into the analysis of an internal survival data. We also provide another function Print.SMC.AuxSP() for printing the results with a better presentation. Detailed usages are described, and implementations are illustrated with numerical examples, including a simulated dataset with a well-designed data generating process and a real breast cancer dataset. Substantial efficiency gain can be observed by our results. CONCLUSIONS: Our R package CureAuxSP can make the wide applications of utilizing auxiliary information possible. It is anticipated that the performance of mixture cure models can be improved for the survival data with a cure fraction, especially for those with small sample sizes.


Probability , Proportional Hazards Models , Software , Humans , Survival Analysis , Models, Statistical , Computer Simulation , Algorithms , Breast Neoplasms/mortality , Breast Neoplasms/therapy
13.
Virus Res ; 346: 199408, 2024 May 28.
Article En | MEDLINE | ID: mdl-38797342

Noroviruses are a group of non-enveloped single-stranded positive-sense RNA virus belonging to Caliciviridae family. They can be transmitted by the fecal-oral route from contaminated food and water and cause mainly acute gastroenteritis. Outbreaks of norovirus infections could be difficult to detect and investigate. In this study, we developed a simple threshold detection approach based on variations of the P2 domain of the capsid protein. We obtained sequences from the norovirus hypervariable P2 region using Sanger sequencing, including 582 pairs of epidemiologically-related strains from 35 norovirus outbreaks and 6402 pairs of epidemiologically-unrelated strains during the four epidemic seasons. Genetic distances were calculated and a threshold was performed by adopting ROC (Receiver Operating Characteristic) curve which identified transmission clusters in all tested outbreaks with 80 % sensitivity. In average, nucleotide diversity between outbreaks was 67.5 times greater than the diversity within outbreaks. Simple and accurate thresholds for detecting norovirus transmissions of three genotypes obtained here streamlines molecular investigation of norovirus outbreaks, thus enabling rapid and efficient responses for the control of norovirus.

14.
PLoS One ; 19(5): e0303696, 2024.
Article En | MEDLINE | ID: mdl-38787895

Most of the existing low-light image enhancement methods suffer from the problems of detail loss, color distortion and excessive noise. To address the above-mentioned issues, this paper proposes a neural network-based low-light image enhancement network. The network is divided into three parts: decomposition network, reflection component denoising network, and illumination component enhancement network. In the decomposition network, the input image is decomposed into a reflection image and an illumination image. In the reflection component denoising network, the Unet3+ network improved by fusion CA attention is adopted to denoise the reflection image. In the illumination component enhancement network, the adaptive mapping curve is adopted to enhance the illumination image iteratively. Finally, the processed illumination and reflection images are fused based on Retinex theory to obtain the final enhanced image. The experimental results show that the proposed network achieves excellent visual effects in subjective evaluation. Additionally, it shows a significant improvement in objective evaluation metrics, including PSNR, SSIM, NIQE, and so on, when compared to the results in several public datasets.


Lighting , Neural Networks, Computer , Lighting/methods , Humans , Image Enhancement/methods , Image Processing, Computer-Assisted/methods , Algorithms , Light
15.
Sci Total Environ ; 937: 173482, 2024 Aug 10.
Article En | MEDLINE | ID: mdl-38795982

Extensive application of rare earth element oxide nanoparticles (REE NPs) has raised a concern over the possible toxic health effects after human exposure. Once entering the body, REE NPs are primarily processed by phagocytes in particular macrophages and undergo biotic phosphate complexation in lysosomal compartment. Such biotransformation affects the target organs and in vivo fate of REE NPs after escaping the lysosomes. However, the immunomodulatory effects of intraphagolysosomal dissolved REE NPs remains insufficient. Here, europium oxide (Eu2O3) NPs were pre-incubated with phagolysosomal simulant fluid (PSF) to mimic the biotransformation of europium oxide (p-Eu2O3) NPs under acid phagolysosome conditions. We investigated the alteration in immune cell components and the hematopoiesis disturbance on adult mice after intravenous administration of Eu2O3 NPs and p-Eu2O3 NPs. Our results indicated that the liver and spleen were the main target organs for Eu2O3 NPs and p-Eu2O3 NPs. Eu2O3 NPs had a much higher accumulative potential in organs than p-Eu2O3 NPs. Eu2O3 NPs induced more alterations in immune cells in the spleen, while p-Eu2O3 NPs caused stronger response in the liver. Regarding hematopoietic disruption, Eu2O3 NPs reduced platelets (PLTs) in peripheral blood, which might be related to the inhibited erythrocyte differentiation in the spleen. By contrast, p-Eu2O3 NPs did not cause significant disturbance in peripheral PLTs. Our study demonstrated that the preincubation with PSF led to a distinct response in the immune system compared to the pristine REE NPs, suggesting that the potentially toxic effects induced by the release of NPs after phagocytosis should not be neglected, especially when evaluating the safety of NPs application in vivo.


Europium , Hematopoiesis , Lysosomes , Metal Nanoparticles , Oxides , Animals , Europium/toxicity , Mice , Lysosomes/drug effects , Lysosomes/metabolism , Oxides/toxicity , Hematopoiesis/drug effects , Metal Nanoparticles/toxicity , Spleen/drug effects , Nanoparticles/toxicity
16.
Biochem Biophys Res Commun ; 719: 150084, 2024 Jul 30.
Article En | MEDLINE | ID: mdl-38733742

INTRODUCTION: Colorectal cancer (CRC) is a prevalent digestive malignancy with significant global mortality and morbidity rates. Improving diagnostic capabilities for CRC and investigating novel therapeutic approaches are pressing clinical imperatives. Additionally, carcinoembryonic antigen (CEA) has emerged as a highly promising candidate for both colorectal tumor imaging and treatment. METHODS: A novel active CEA-targeting nanoparticle, CEA(Ab)-MSNs-ICG-Pt, was designed and synthesized, which served as a tumor-specific fluorescence agent to help in CRC near-infrared (NIR) fluorescence imaging. In cell studies, CEA(Ab)-MSNs-ICG-Pt exhibited specific targeting to RKO cells through specific antibody-antigen binding of CEA, resulting in distribution both within and around these cells. The tumor-targeting-specific imaging capabilities of the nanoparticle were determined through in vivo fluorescence imaging experiments. Furthermore, the efficacy of the nanoparticle in delivering chemotherapeutics and its killing effect were evaluated both in vitro and in vivo. RESULTS: The CEA(Ab)-MSNs-ICG-Pt nanoparticle, designed as a novel targeting agent for carcinoembryonic antigen (CEA), exhibited dual functionality as a targeting fluorescent agent. This CEA-targeting nanoparticle showed exceptional efficacy in eradicating CRC cells in comparison to individual treatment modalities. Furthermore, it exhibits exceptional biosafety and biocompatibility properties. CEA(Ab)-MSNs-ICG-Pt exhibits significant promise due to its ability to selectively target tumors through NIR fluorescence imaging and effectively eradicate CRC cells with minimal adverse effects in both laboratory and in vivo environments. CONCLUSION: The favorable characteristics of CEA(Ab)-MSNs-ICG-Pt offer opportunities for its application in chemotherapeutic interventions, tumor-specific NIR fluorescence imaging, and fluorescence-guided surgical procedures.


Carcinoembryonic Antigen , Colorectal Neoplasms , Nanoparticles , Carcinoembryonic Antigen/metabolism , Colorectal Neoplasms/diagnostic imaging , Colorectal Neoplasms/drug therapy , Colorectal Neoplasms/pathology , Colorectal Neoplasms/metabolism , Nanoparticles/chemistry , Humans , Animals , Cell Line, Tumor , Optical Imaging/methods , Mice , Mice, Nude , Antineoplastic Agents/pharmacology , Antineoplastic Agents/chemistry , Antineoplastic Agents/therapeutic use , Mice, Inbred BALB C , Fluorescent Dyes/chemistry
17.
J Hazard Mater ; 473: 134579, 2024 May 12.
Article En | MEDLINE | ID: mdl-38761761

Ciprofloxacin (CIP) has received considerable attention in recent decades due to its high ecological risk. However, little is known about the potential response of macrophytes and microbes to varying levels of CIP exposure in constructed wetlands. Therefore, lab-scale manganese ore-based tidal flow constructed wetlands (MO-TFCWs) were operated to evaluate the responses of macrophytes and microbes to CIP over the long term. The results indicated that total nitrogen removal improved from 79.93% to 87.06% as CIP rose from 0 to 4 mg L-1. The chlorophyll content and antioxidant enzyme activities in macrophytes were enhanced under CIP exposure, but plant growth was not inhibited. Importantly, CIP exposure caused a marked evolution of the substrate microbial community, with increased microbial diversity, expanded niche breadth and enhanced cooperation among the top 50 genera, compared to the control (no CIP). Co-occurrence network also indicated that microorganisms may be more inclined to co-operate than compete. The abundance of the keystone bacterium (involved in nitrogen transformation) norank_f__A0839 increased from 0.746% to 3.405%. The null model revealed drift processes (83.33%) dominated the community assembly with no CIP and 4 mg L-1 CIP. Functional predictions indicated that microbial carbon metabolism, electron transfer and ATP metabolism activities were enhanced under prolonged CIP exposure, which may contribute to nitrogen removal. This study provides valuable insights that will help achieve stable nitrogen removal from wastewater containing antibiotic in MO-TFCWs.

18.
Environ Sci Ecotechnol ; 20: 100412, 2024 Jul.
Article En | MEDLINE | ID: mdl-38560759

Effective management of large basins necessitates pinpointing the spatial and temporal drivers of primary index exceedances and urban risk factors, offering crucial insights for basin administrators. Yet, comprehensive examinations of multiple pollutants within the Yangtze River Basin remain scarce. Here we introduce a pollution inventory for urban clusters surrounding the Yangtze River Basin, analyzing water quality data from 102 cities during 2018-2019. We assessed the exceedance rates for six pivotal indicators: dissolved oxygen (DO), ammonia nitrogen (NH3-N), chemical oxygen demand (COD), biochemical oxygen demand (BOD), total phosphorus (TP), and the permanganate index (CODMn) for each city. Employing random forest regression and SHapley Additive exPlanations (SHAP) analyses, we identified the spatiotemporal factors influencing these key indicators. Our results highlight agricultural activities as the primary contributors to the exceedance of all six indicators, thus pinpointing them as the leading pollution source in the basin. Additionally, forest coverage, livestock farming, chemical and pharmaceutical sectors, along with meteorological elements like precipitation and temperature, significantly impacted various indicators' exceedances. Furthermore, we delineate five core urban risk components through principal component analysis, which are (1) anthropogenic and industrial activities, (2) agricultural practices and forest extent, (3) climatic variables, (4) livestock rearing, and (5) principal polluting sectors. The cities were subsequently evaluated and categorized based on these risk components, incorporating policy interventions and administrative performance within each region. The comprehensive analysis advocates for a customized strategy in addressing the discerned risk factors, especially for cities presenting elevated risk levels.

19.
Hum Vaccin Immunother ; 20(1): 2337157, 2024 Dec 31.
Article En | MEDLINE | ID: mdl-38644633

This study aimed to investigate the knowledge about, attitudes toward, and acceptance and predictors of receiving the mpox vaccine among Chinese cancer patients. Patients were selected using a convenience sampling method. A web-based self-report questionnaire was developed to assess cancer patients' knowledge, attitudes, and acceptance regarding the mpox vaccine. Multivariate logistic regression analysis was used to determine predictors of acceptance of the mpox vaccine. A total of 805 cancer patients were included in this study, with a vaccine hesitancy rate of 27.08%. Approximately 66% of the patients' information about mpox and the vaccine came from the mass media, and there was a significant bias in the hesitant group's knowledge about mpox and the vaccine. Multivariable logistic regression analysis suggested that retirement; chemotherapy; the belief that the mpox vaccine could prevent disease, that vaccination should be compulsory when appropriate and that the mpox vaccine prevents mpox and reduces complications; the willingness to pay for the mpox vaccine; the willingness to recommend that friends and family receive the mpox vaccine; and the belief that the mpox vaccine should be distributed fairly and equitably were factors that promoted vaccination. The belief that mpox worsens tumor prognosis was a driving factor for vaccine hesitancy. This study investigated the knowledge of cancer patients about mpox and the vaccine, evaluated the acceptance and hesitancy rates of the mpox vaccine and examined the predictors of vaccination intention. We suggest that the government scientifically promote the vaccine and develop policies such as free vaccination and personalized vaccination to increase the awareness and acceptance rate of the mpox vaccine.


Health Knowledge, Attitudes, Practice , Neoplasms , Patient Acceptance of Health Care , Humans , Male , Female , China , Cross-Sectional Studies , Middle Aged , Neoplasms/psychology , Adult , Patient Acceptance of Health Care/psychology , Surveys and Questionnaires , Aged , Cancer Vaccines , Vaccination Hesitancy/psychology , Vaccination Hesitancy/statistics & numerical data , Vaccination/psychology , Vaccination/statistics & numerical data , Intention , Young Adult
20.
Heliyon ; 10(8): e29472, 2024 Apr 30.
Article En | MEDLINE | ID: mdl-38644826

Research in Positive Psychology has indicated a correlation between Psychological Capital (PsyCap) and Mental health (MH). However, the specific contribution of Perceived Social Support (PSS) in the connection between PsyCap and MH, particularly within higher education, remains uninvestigated. This study investigated how PSS could mediate the effect of PsyCap on students' MH using a cross-sectional research design. The sample encompassed 443 undergraduate graduate students at Huazhong University of Science and Technology in Wuhan, China. Results from Partial Least Squares Structural Equation Modeling (PLS-SEM) showed that both PsyCap (ß = 0.815, t = 31.074, p < 0.000) and PSS (ß = 0.405, t = 28.051, p < 0.000) have a positive impact on students' MH. Additionally, PSS was identified as a significant mediator in relation to students' MH (b = 0.080, t = 2.319, p < 0.020). This study emphasizes the importance of developing these factors in educational and support programs to enhance students' well-being. Moreover, the results offer significant conceptual and practical insights for higher education faculty, psychologists, and curriculum designers.

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