ABSTRACT
The study evaluated the effects of R-limonene, in sublethal concentration, on the histology, histochemistry, biochemistry, and carbohydrates and proteins levels in the third instar Aedes aegypti larvae. The R-limonene (LC50 of 27 ppm) and control groups were analyzed 12 and 24 h after the beginning of treatments. The midgut of the control larvae was composed of cylindrical and elongated cells with a spherical and central nucleus and regenerative cells with a pyramidal shape. After 12 h of treatment, columnar cells, protuberances, and cytoplasmic vacuolization were found. However, 24 h after treatment, complete disorganization of the epithelium was observed. There was a positive reaction in all treatments for the presence of glycogen. However, the midgut of larvae treated with R-limonene showed higher levels. For the total protein, positive marking occurred in all groups evaluated, with higher levels in treatments and the lowest in control 12 h. The levels of total protein and glycogen increased in the treated larvae compared to the 12 h control. Besides, a reduction in total sugar levels was observed in the treated larvae compared to controls 12 and 24 h, being more evident in the last one. Therefore, these results demonstrate that R-Limonene caused pathological changes in the epithelium of the A. aegypti midgut at histophysiological and biochemical levels.
Subject(s)
Aedes , Insecticides , Limonene , Animals , Larva , Plant ExtractsABSTRACT
Spodoptera frugiperda is a major pest in several crops due to its polyphagous habit. Studies on the use of essential oils for pest control have been increasing over the years, presenting itself as a promising alternative with less environmental impact. Chemical profile evaluations of essential oils enable the knowledge and use of major compounds, providing a better understanding of their actions in the life history of insects. Thus, the study evaluated the effects of the major compounds limonene, trans-anethole and the combined effects upon nutrition, reproduction and testicular apoptosis in S. frugiperda. Larvae of third instar were submitted to the LD50 of the compounds by topical contact and 48 h later the amounts of lipids, proteins, total sugar and glycogen were evaluated. The testicular apoptosis was evaluated in the treated larvae after 48 h and the reproductive parameters were evaluated after adult emergence. All treatments reduced the amounts of lipid, protein, total sugar and glycogen when compared to control. The most significant results were obtained by the association of compounds. All treatments reduced the number of eggs, oviposition period and adult longevity when compared to control. There were no changes in the pre-oviposition and post-oviposition periods. Testicular apoptosis was observed in the limonene and in the combined treatments. It is concluded that limonene and trans-anethole, especially in association, cause adverse effects upon nutrition and reproduction in S. frugiperda, altering essential parameters for its survival and establishment on crops.
Subject(s)
Anisoles/toxicity , Cyclohexenes/toxicity , Reproduction/drug effects , Spodoptera/drug effects , Terpenes/toxicity , Testis/drug effects , Allylbenzene Derivatives , Animals , Anisoles/chemistry , Apoptosis/drug effects , Cyclohexenes/chemistry , Isomerism , Larva/drug effects , Lethal Dose 50 , Limonene , Male , Microscopy, Fluorescence , Oils, Volatile/chemistry , Spodoptera/growth & development , Terpenes/chemistry , Testis/cytology , Testis/metabolismABSTRACT
Dengue fever has spread worldwide and affects millions of people every year in tropical and subtropical regions of Africa, Asia, Europe and America. Since there is no effective vaccine against the dengue virus, prevention of disease transmission depends entirely on regulating the vector (Aedes aegypti) or interrupting human-vector contact. The aim of this study was to assess the oviposition deterrent activity of essential oils of three cultivars of torch ginger (Etlingera elatior, Zingiberaceae) against the dengue mosquito. Analysis of the oils by gas chromatography (GC)-mass spectrometry revealed the presence of 43 constituents, of which α-pinene, dodecanal and n-dodecanol were the major components in all cultivars. Solutions containing 100 ppm of the oils exhibited oviposition deterrent activities against gravid Ae. aegypti females. GC analysis with electroantennographic detection indicated that the oil constituents n-decanol, 2-undecanone, undecanal, dodecanal, trans-caryophyllene, (E)-ß-farnesene, α-humulene, n-dodecanol, isodaucene and dodecanoic acid were able to trigger antennal depolarization in Ae. aegypti females. Bioassays confirmed that solutions containing 50 ppm of n-dodecanol or dodecanal exhibited oviposition deterrent activities, while a solution containing the alcohol and aldehyde in admixture at concentrations representative of the oil presented an activity similar to that of the 100 ppm oil solution. Docking and molecular dynamics simulations verified that the interaction energies of the long-chain oil components and Ae. aegypti odorant binding protein 1 were quite favorable, indicating that the protein is a possible oviposition deterrent receptor in the antenna of Ae. aegypti.
Subject(s)
Aedes/drug effects , Behavior, Animal/drug effects , Electrophysiological Phenomena/drug effects , Flowers/chemistry , Molecular Dynamics Simulation , Oils, Volatile/pharmacology , Zingiberaceae/chemistry , Aedes/metabolism , Aedes/physiology , Animals , Arthropod Antennae/drug effects , Arthropod Antennae/physiology , Dengue/transmission , Female , Humans , Insect Proteins/chemistry , Insect Proteins/metabolism , Insect Vectors/drug effects , Insect Vectors/metabolism , Insect Vectors/physiology , Oils, Volatile/chemistry , Oils, Volatile/metabolism , Protein ConformationABSTRACT
A set of aryl- and phenoxymethyl-(thio)semicarbazones were synthetized, characterized and biologically evaluated against the larvae of Aedes aegypti (A. aegypti), the vector responsible for diseases like Dengue and Yellow Fever. (Q)SAR studies were useful for predicting the activities of the compounds not included to create the QSAR model as well as to predict the features of a new compound with improved activity. Docking studies corroborated experimental evidence of AeSCP-2 as a potential target able to explain the larvicidal properties of its compounds. The trend observed between the in silico Docking scores and the in vitro pLC50 (equals -log LC50, at molar concentration) data indicated that the highest larvicidal compounds, or the compounds with the highest values for pLC50, are usually those with the higher docking scores (i.e., greater in silico affinity for the AeSCP-2 target). Determination of cytotoxicity for these compounds in mammal cells demonstrated that the top larvicide compounds are non-toxic.
Subject(s)
Aedes/drug effects , Carrier Proteins/antagonists & inhibitors , Thiosemicarbazones/pharmacology , Animals , Dose-Response Relationship, Drug , Larva/drug effects , Mice , Mice, Inbred BALB C , Molecular Structure , Quantitative Structure-Activity Relationship , Spleen/cytology , Thiosemicarbazones/chemical synthesis , Thiosemicarbazones/chemistryABSTRACT
The larvicidal activities of extracts of three hardwood species (Hymenaea stigonorcapa, Anadenanthera colubrina and Bowdichia virgilioides) against 4th instar larvae of Aedes aegypti were evaluated using WHO guidelines. Extracts of H. stignocarpa and A. colubrina showed weak activity. The highest larvicidal effect was obtained with the cyclohexane extract of the heartwood of B. virgilioides, which caused 100% mortality at concentrations at 50 and 100 µg/mL. Fraction toluene/EtOAc (8:2) from this extract showed larvicidal activity (LC50 = 34.90 ± 1.27 µg/mL). A mixture of two compounds identified as medicarpin and maackiain exhibited a very good larvicidal activity (sub-fraction 2, LC50 = 17.5 ± 1.87 µg/mL) and maackiain showed to be a strong larvicidal compound (LC50 = 21.95 ± 1.34 µg/mL). This result can be of value in the search for new natural larvicidal compounds from other hardwood plant extracts and presents the first report of B. virgilioides being used to control a mosquito vector.
Subject(s)
Aedes/drug effects , Fabaceae/chemistry , Insecticides/pharmacology , Larva/drug effects , Plant Extracts/pharmacology , Pterocarpans/pharmacology , Aedes/growth & development , Animals , Insect Vectors/drug effects , Insect Vectors/growth & development , Larva/growth & developmentABSTRACT
Although numerous reports are available concerning the larvicidal potential of essential oils, very few investigations have focused on their mechanisms of action. In the present study, we have investigated the chemical composition of the leaf oil of Croton rhamnifolioides during storage and its effects on oviposition and survival of larvae of the dengue fever mosquito Aedes aegypti. In addition, we have established a possible mechanism of action for the larvicidal activity of the essential oil. GC-MS analyses revealed marked differences in the composition of oil that had been freshly isolated and that of a sample that had been stored in a sealed amber-glass vial under refrigeration for three years. However, both fresh and stored oil exhibited substantial larvicidal activities with LC50 values of 122.35 and 89.03 ppm, respectively, and oviposition deterrent effects against gravid females at concentrations of 50 and 100 µg·mL-1. These results demonstrate that the larvicidal effect of the essential oil was unchanged during three years of storage even though its chemical composition altered. Hence, the essential oil could be used in the preparation of commercial products. In addition, we observed that the trypsin-like activity of mosquito larvae was inhibited in vitro by the essential oil of C. rhamnifolioides, suggesting that the larvicidal effect may be associated with inhibition of this enzyme.
Subject(s)
Aedes/anatomy & histology , Croton/chemistry , Oils, Volatile/pharmacology , Oviposition/drug effects , Trypsin/metabolism , Aedes/drug effects , Animals , Croton Oil/pharmacology , Female , Gas Chromatography-Mass Spectrometry , Larva/drug effects , Plant Leaves/chemistryABSTRACT
The mosquito Aedes aegypti is the vector agent responsible for the transmission of yellow fever and dengue fever viruses to over 80 million people in tropical and subtropical regions of the world. Exhaustive efforts have lead to a vaccine candidate with only 30% effectiveness against the dengue virus and failure to protect patients against the serotype 2. Hence, vector control remains the most viable route to dengue fever control programs. We have synthesized a class of 1,2,4-oxadiazole derivatives whose most biologically active compounds exhibit potent activity against Aedes aegypti larvae (ca. of 15 ppm) and low toxicity in mammals. Exposure to these larvicides results in larvae pigmentation in a manner correlated with the LC50 measurements. Structural comparisons of the 1,2,4-oxadiazole nucleus against known inhibitors of insect enzymes allowed the identification of 3-hydroxykynurenine transaminase as a potential target for these synthetic larvicides. Molecular docking calculations indicate that 1,2,4-oxadiazole compounds can bind to 3-hydroxykynurenine transaminase with similar conformation and binding energies as its crystallographic inhibitor 4-(2-aminophenyl)-4-oxobutanoic acid.