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Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
; 32(5): 577-80, 2016 May.
Article
in Chinese
| MEDLINE
| ID: mdl-27126931
ABSTRACT
OBJECTIVE: To investigate the impact of interferon γ (IFN-γ) on cathepsin S (CTSS) expressed by P815 mouse mast cells and mouse bone marrow-derived mast cells (BMMCs). METHODS: IFN-γ of 10, 25 and 50 ng/mL were respectively used to stimulate P815 cells and mouse BMMCs. Real-time PCR and ELISA were applied to detect mRNA and protein levels of CTSS in P815 cells and BMMCs. 25 ng/mL IFN-γ was used to treat P815 cells for 6, 12, 18, 24, 48 and 72 hours and mouse BMMCs for 12, 24 and 48 hours; the above mentioned detection steps were then repeated. RESULTS: IFN-γ induced P815 cells and BMMCs to express CTSS on the mRNA and protein levels in the time- and dose-dependent manners. CONCLUSION: IFN-γ could stimulate mast cells to release CTSS.