The Least Nighthawk Chordeiles pusillus is widespread wherever there are savannas in the South American tropics, often in isolated patches, such as white-sands savannas in the Amazon rainforest realm. Here, we investigate genetic relationships between populations of the Least Nighthawk to understand historical processes leading to its diversification and to determine dispersal routes between northern and southern savannas by way of three hypothesized dispersal corridors by comparing samples from white-sand savannas to samples from other savannas outside of the Amazon rainforest region. We use 32 mtDNA samples from the range of C. pusillus to infer a dated phylogeny. In a subset of 17 samples, we use shotgun sequences to infer a distance-based phylogeny and to estimate individual admixture proportions. We calculate gene flow and shared alleles between white-sand and non-Amazonian populations using the ABBA-BABA test (D statistics), and Principal Component Analysis (PCA) to examine genetic structure within and between lineages. Finally, we use species distribution modelling (SDM) of conditions during the Last Glacial Maximum (LGM), currently, and in the future (2050-2080) to predict potential species occurrence under a climate change scenario. Two main clades (estimated to have diverged around 1.07 million years ago) were recovered with mtDNA sequences and Single Nucleotide Polymorphism (SNPs) and were supported by NGSadmix and PCA: one in the Amazon basin white-sand savannas, the other in the non-Amazonian savannas. Possible allele sharing between these clades was indicated by the D-statistics between northern non-Amazonian populations and the white-sand savanna population, but this was not corroborated by the admixture analyses. Dispersal among northern non-Amazonian populations may have occurred in a dry corridor between the Guianan and the Brazilian Shield, which has since moved eastward. Our data suggest that the lineages separated well before the Last Glacial Maximum, consequently dispersal could have happened at any earlier time during similar climatic conditions. Subsequently, non-Amazonian lineages became more divergent among themselves, possibly connecting and dispersing across the mouth of the Amazon River across Marajó island during favourable climatic conditions in the Pleistocene.
The fluvial transport of dissolved inorganic carbon (DIC) is an important component of the global carbon cycle. Herein, we assessed the dynamics of DIC and the C stable isotopic composition (δ13CDIC) in a watershed with diversified land use in São Paulo State (Brazil), more specifically in the Sorocaba River basin (SRB) and considered the temporal and spatial scales. For this purpose, twelve fluvial samples at each sampling point (e.g., S1, S2, S3, S4 and S5) were collected in the SRB, from June 2009 to May 2010, which represented one complete hydrological cycle that included the extremes of the rainfall and discharge regimes. In addition, the δ13CDIC values were also characterized in the wet and dry season at all sampling points to verify their seasonal variability. The results reflected the seasonal variation in discharges, water temperatures, and electrical conductivities. Most of the DIC was transported in the wet season at all sampling points, where the less negative δ13CDIC values were characterized. The natural sources of DIC are associated with atmospheric/soil CO2 consumption. The anthropogenic impacts on both [DIC] and δ13CDIC are linked to untreated urban sewage that is discharged directly into the Sorocaba River, as well as to aquatic photosynthesis in the Itupararanga Reservoir. From 1970 to 2020, the modeling proposed in this study indicated that the annual flux of DIC (Friver) varied from 9.0 to 78.7 t km-2 a-1, confirming that the El Niño Southern Oscillation (ENSO) controlled Friver in the SRB, with higher and lower Friver values occurring during strong El Niño (EN) and La Niña (LN) years. The average Friver value was 20 t km-2 a-1, which is higher than those obtained in natural several temperate and tropical watersheds due to the influences of urban areas on [DIC] in the SRB.
In this paper, we conducted a meta-analytic review to examine the impact of social presence on individuals' performance on the Stroop task, shedding light on the cognitive processes underlying social facilitation. We followed PRISMA guidelines to identify and include 33 relevant studies in a multivariate random-effects meta-analysis. Our results show that social presence reliably modulates Stroop interference (a measure of cognitive control); specifically, participants exhibit lower Stroop interference when performing the task in the presence of others compared to performing it in isolation. We also found that the strength of the effect varies depending on the type of social presence: it is stronger with an attentive audience compared to an inattentive one, and null with an evaluative audience. Additionally, different features of the Stroop task itself moderate the effect; the effect is stronger for the classic version of the task compared to the semantic version, and for experiments that use mixed within-block trials compared to those with homogenous blocks. We also observed a negative relationship between the number of trials and the magnitude of the effect. Overall, these findings provide insights into the mechanisms by which the presence of others affects performance on the Stroop task, and how they align with social facilitation theories.
The innate immune system of mammals is formed by a complex web of interacting proteins, which together constitute the first barrier of entry for infectious pathogens. Genes from the E3-ubiquitin ligase tripartite motif (TRIM) family have been shown to play an important role in the innate immune system by restricting the activity of different retrovirus species. For example, TRIM5 and TRIM22 have both been associated with HIV restriction and are regarded as crucial parts of the antiretroviral machinery of mammals. Our analyses of positive selection corroborate the great significance of these genes for some groups of mammals. However, we also show that many species lack TRIM5 and TRIM22 altogether. By analyzing a large number of mammalian genomes, here we provide the first comprehensive view of the evolution of these genes in eutherians, showcasing that the pattern of accumulation of TRIM genes has been dissimilar across mammalian orders. Our data suggest that these differences are caused by the evolutionary plasticity of the immune system of eutherians, which have adapted to use different strategies to combat retrovirus infections. Altogether, our results provide insights into the dissimilar evolution of a representative family of restriction factors, highlighting an example of adaptive and idiosyncratic evolution in the innate immune system.
Antiviral Restriction Factors , Proteins , Animals , Tripartite Motif Proteins/genetics , Proteins/genetics , Ubiquitin-Protein Ligases/genetics , Mammals/genetics , Mammals/metabolism , Eutheria/metabolism
Although muscle injuries represent the most frequent injury in professional football, isolated complete distal ruptures of the rectus femoris (RF) muscle are rare, and there is no consensus on their treatment and return to play (RTP). In this article, we report a clinical case of successful non-surgical management of an RF grade 4c muscle injury in a professional football player, in which the athlete was able to RTP 21 weeks after the injury, had no re-injury >1 year after RTP, and is playing at an elite level in the Portuguese Football First League.
INTRODUCTION: People's estimates of perceptual quantities are commonly biased by the contextual presence of other quantities (like numbers). In this study, we address assimilation anchoring effects (approximation of real quantities to contextual quantities) that occur for visually displayed proportions, defining a new methodological setting for the effect. METHOD: Similar to classic approaches, we asked participants across several trials whether the display contained a feature in a proportion higher or lower than "a randomly selected value" (relative judgments), and then estimated the feature proportions (absolute judgments). Across all trials, we presented seven anchors ranging from .20 to .80, each with a visually displayed representation of the same seven proportions (49 judgments in total). This allowed for a psychophysical approach to individual estimates and signal detection indexes, providing new insights into how the anchoring effect is generated in this setting. RESULTS: Our findings suggest that anchoring effects occur both as a bias (changes in response criteria) and as a change in the ability to discriminate stimuli (affecting sensitivity indexes). Moreover, anchors modulate the level of stimuli features for which estimates were more uncertain. Finally, our results indicate that anchor effects occur immediately in the first phase of the two-phase paradigm, leading to the availability of values for supporting absolute estimates. CONCLUSION: By using a psychophysical approach to the anchoring effects, for the first time, we could clarify that this effect is the result of both bias and changes in the ability to discriminate quantity.
Judgment , Humans , Uncertainty
This article addresses a gap in the literature regarding the design and implementation of health and well-being strategies by local governments. It presents a conceptual framework that can help local governments to include health and well-being among their political priorities by promoting the participation of local stakeholders and the community in general. The article also highlights the important role that local governments play in public health, especially in times of crisis, such as the COVID-19 pandemic. To demonstrate the approach, the manuscript explores the recent shift toward decentralization in Portugal and the resulting emphasis on local governments leading new approaches to health governance. The planning process for Arouca's Health Strategy, an inland municipality, is used as a case study to demonstrate the approach, which emphasizes the meaningful participation of the entire local community. The article concludes by noting that there is still significant room for improvement in all areas related to health and well-being and a need to adopt new multistakeholder governance arrangements to sustain the institutional capacity for upcoming strategies.
COVID-19 , Local Government , Humans , Pandemics/prevention & control , COVID-19/epidemiology , COVID-19/prevention & control , Cities , Portugal
In professional football, most of the injuries are traumatic; however, these athletes may suffer from rheumatologic diseases, that may present as sports-related injuries. Reactive arthritis (ReA) is classified as a sub-group of the spondyloarthritis family and is relatively rare. In this article, we highlight the successful return to play (RTP) process after the ReA diagnosis in an elite football player in the Portuguese first league. The athlete was able to RTP four months and one week after the diagnosis, had no ReA recurrence nor re-injury >8 months after RTP, and is playing at an elite level.
This research was carried out with the objective of evaluating the effects of using a chilling water sprinkler system during the cooling process of swine carcasses on the quantitative and qualitative parameters of carcass and meat. A total of 220 swine carcasses were divided in a completely randomized experiment and two treatments: (1) CONTROL, no water spraying; (2) SPRAY, with water spraying during cooling. Surface and internal temperature of carcasses throughout the cooling process, initial and final pH, and microbiological analyses of carcass surface were evaluated. Samples of the Longissimus lumborum (LL) were collected for analysis of color, cooking loss (CL), shear force (SF), and drip loss (DL). Data were submitted to analysis of variance through the SAS MIXED procedure adopting the most adequate model with treatments as fixed effects and pertinent random effects for each data set. The use of spray-chilling in the initial cooling process accelerates the surface and internal temperature decrease of swine carcasses, which may be a viable technological resource in swine industry.
Cold Temperature , Food Handling , Animals , Cooking , Food Handling/methods , Meat/analysis , Swine , Temperature
Deontic norms are expected to impose individuals' control over their behavior. In this paper, we address such norms presented in traffic signs and test their influence over executive control functions. For Experiment 1, we develop a traffic flanker task in which the typical neutral arrows are replaced with traffic prohibition/obligation signs. Experiment 2 isolated the deontic aspect of the signs using simple arrows on red, blue, and green backgrounds and either primed them to be interpreted as traffic signs or as elements of a gaming console controller. Results in both studies show evidence of controlling context interferences more efficiently when dealing with deontic (traffic) signs than with simple arrows (Experiment 1) or with similar perceptive targets when primed with a deontic context than with a gaming context (Experiment 2). In both studies, obligation/blue signs mitigate flanker effects less than prohibition/red signs. Stimuli color affects the alertness of the cognitive system, with the color red being, by itself, a cue for increased control. Based on temporal analysis, we further discuss these results as evidence of an increase in proactive control that aims to prevent the occurrence of undesirable influence.
Attention , Executive Function , Humans
Studies on long coronavirus disease (COVID) in children are scarce. We aimed to describe persistent symptoms and identify risk factors for its development. In our study population, 17.6% presented with long COVID, with respiratory symptoms more frequent in the first weeks and neuropsychiatric symptoms over time. Chronic conditions and obesity were risk factors, and adolescents were at a greater risk for long COVID.
COVID-19 , Post-Acute COVID-19 Syndrome , Humans , Adolescent , Child , Retrospective Studies , COVID-19/epidemiology , Risk Factors
Inhibitory control (IC) is defined as the (in)ability to change, suppress, or delay a response that is no longer required under the current circumstances. This ability was previously argued to increase in social contexts, based on Stroop's performance, showing that participants performed the Stroop task better in others' presence than alone. In this paper, we extend the testing of this same hypothesis to the use of two other tasks that Mitake et al. (2000) show to grasp the same IC ability; the Antisaccade and Stop signal tasks. If Stroop's performance was capturing the impact of the presence of others on CI abilities, the effect would generalize to performance on these tasks. This hypothesis was only generally supported by stop signal task performance; those in the presence condition were significantly more efficient than those in the alone conditions. For the Antisaccade tasks, evidence shows that higher levels of interference occurs in the presence of others condition for participants' fastest responses We discuss how this evidence contributes to the literature suggesting that the two tasks may index different constructs.
The purpose of the study is to describe the experience of a multidisciplinary team in a tertiary hospital regarding the management of Infantile Hemangiomas (IH). The method employed is a retrospective analysis of patients with IH followed in a tertiary pediatric hospital between January 2010 and May 2022. A total of 393 IH were diagnosed (56.7% female), with a median age of 5 months (interquartile range (IQR), 3-10). Imaging investigation was necessary for diagnosis and for exclusion of other IH in 9.2% and 14.3%, respectively. Focal (74.0%) and superficial (59.7%) lesions were more frequent as was facial location (35.9%). Pre-treatment ulceration or hemorrhage occurred in 6.6%. At follow-up, 87.4% regressed partially and 12.6% completely; 2.7% relapsed. Propranolol was started in 30.0% of cases for a median period of 9 months (IQR, 6-12), mainly due to esthetic concerns (41.9%). Side effects occurred in 8.3% (sleep disturbance in 5.1%). Only 1.7% were refractory and 5.9% had a rebound effect. Eleven patients were treated with topical timolol and 41 underwent surgery. Patients that were treated with propranolol had more risk factors (p = 0.016) and presented deeper lesions (p < 0.001) with a larger diameter (p < 0.001); total IH regression was less frequent (p < 0.001). Since 2020, twice-daily dosage was more frequently prescribed than three times daily (p = 0.007) and inpatient initiation of propranolol decreased (p = 0.750), without significant difference in the incidence of adverse reactions, duration of treatment, and lesion evolution. Conclusions: Our protocol proved to be safe and feasible in an outpatient setting and twice daily administration of propranolol was effective. The majority of IH showed at least partial regression. Early detection of high-risk IH is paramount and a multidisciplinary assessment by a specialized team is essential for adequate management. What is Known: ⢠IH are the most common vascular tumors in childhood. Although the majority evolves favorably, treatment may be warranted in selected cases. ⢠Early detection of high-risk IH is paramount, and a multidisciplinary assessment by a specialized team is essential for adequate management. What is New: ⢠One-third of our sample was treated with propranolol. These patients had more risk factors and presented deeper lesions with a larger diameter, and tumor total regression was less frequent. ⢠Our results reinforce safety and feasibility of propranolol initiation in an outpatient setting, including twice daily dosage.
Hemangioma , Skin Neoplasms , Humans , Female , Child , Infant , Male , Propranolol/therapeutic use , Hemangioma/diagnosis , Hemangioma/drug therapy , Retrospective Studies , Tertiary Care Centers , Treatment Outcome , Administration, Oral , Skin Neoplasms/diagnosis , Skin Neoplasms/drug therapy , Adrenergic beta-Antagonists/therapeutic use
INTRODUCTION AND AIMS: Although usually benign, varicella can lead to serious complications and sometimes long-term sequelae. Vaccines are safe and effective but not yet included in immunisation programmes in many countries. We aimed to quantify the impact on health-related quality of life (HRQoL) in terms of quality-adjusted life years (QALY) in children with varicella and their families, key to assessing cost-utility in countries with low mortality due to this infection. METHODS: Children with varicella in the community and admitted to hospitals in Portugal were included over 18 months from January 2019. Children's and carers' HRQoL losses were assessed prospectively using standard multi-attribute utility instruments for measuring HRQoL (EQ-5D and CHU9D), from presentation to recovery, allowing the calculation of QALYs. RESULTS: Among 109 families with children with varicella recruited from attendees at a pediatric emergency service (community arm), the mean HRQoL loss/child was 2.0 days (95 % CI 1.9-2.2, n = 101) (mean 5.4 QALYs/1000 children (95 % CI 5.3-6.1) and 1.3 days/primary carer (95 % CI 1.2-1.6, n = 103) (mean 3.6 QALYs /1000 carers (95 % CI 3.4-4.4). Among 114 families with children admitted to hospital because of severe varicella or a complication (hospital arm), the mean HRQoL loss/child was 9.8 days (95 % CI 9.4-10.6, n = 114) (mean 26.8 QALYs /1000 children (95 % CI 25.8-29.0) and 8.5 days/primary carer (95 % CI 7.4-9.6, n = 114) (mean 23.4 QALYs/1000 carers (95 % CI 20.3-26.2). Mean QALY losses/1000 patients were particularly high for bone and joint infections [67.5 (95 % CI 43.9-97.6)]. Estimates for children's QALYs lost using the CHU9D tool were well correlated with those obtained using EQ-5D, but substantially lower. CONCLUSIONS: The impact of varicella on HRQoL is substantial. We report the first measurements of QALYs lost in hospitalised children and in the families of children both in the community and admitted to hospital, providing important information to guide vaccination policy recommendations.
Chickenpox , Quality of Life , Humans , Child , Quality-Adjusted Life Years , Prospective Studies , Chickenpox/epidemiology , Chickenpox/prevention & control , Portugal , Cost-Benefit Analysis
In the present work, we present normative data for a set of 39 original clipart-style images that can be used as material in studies involving judgements of proportion. The original images are drawings that depict different day-to-day scenarios (e.g., lighted windows in a building; books on a shelf) and each has seven variants of different proportions (from 20% to 80%) belonging to different categories (discrete vs continuous; social vs non-social; natural vs artificial; stimuli physical dimensions; number of referents). Normative data for these images are presented in an interactive database (available at https://judgment-images-and-norms.shinyapps.io/estimates_interactive/ ), corresponding to the means of proportion estimates (in percentage form), the perceived ease of making such estimates, the perceived level of familiarity and liking for each image, and the relationships between these variables. In the paper, we analyse the data at an individual level, addressing how the latter judgements are related to the proportion estimates, how those estimates are related to objective proportions, and how these relationships are moderated by image category. The analyses presented in this paper aim to aid readers in selecting images that enable them to better address specific influences on proportional estimates or to control for those influences in their studies.
Emotions , Judgment , Humans , Databases, Factual , Recognition, Psychology
ABSTRACT Objective: The purpose of the present in vitro study was to evaluate the efficiency of three different instruments in the root canals of artificial primary molars as well as the quality of the fillings. Methods: Sixty artificial primary molars were divided into three groups of 20 teeth. Each group was submitted to a different technique: Group 1 - conventional manual technique using Kerr files; Group 2 - manual technique with nickel-titanium files; Group 3 - NiTi rotary technique. The root canals were filled with calcium hydroxide paste. Aspects such as homogeneity of the fillings, taper and flowability of the preparations were considered comparatively to assess the quality of the instrumentations. The non-parametric Kruskal-Wallis and Mann-Whitney tests were used to compare the efficiency of the preparation of the root canal considering the preparation times of them. Results: All three techniques were equally effective at shaping the root canals of primary teeth. The working time was shorter with the rotary instrument (p=0.000). In the pairwise comparisons, working time was shorter with the rotary instrument compared to the manual techniques with steel files (p=0.000) and NiTi files (p=0.000). Comparing the manual techniques, the working time was shorter with NiTi files compared to steel files (p=0.011). Moreover, less variability in the preparation time was found when the rotary instrument was used. Conclusions: The mechanized rotary method led to a shorter operating time in comparison to the manual techniques and is therefore a preferable option for the preparation of the root canals of primary teeth.
RESUMO Objetivo: Avaliar a eficiência de três instrumentos diferentes nos canais radiculares de molares decíduos artificiais, bem como a qualidade das obturações. Métodos: Sessenta molares decíduos artificiais foram divididos em três grupos de 20 dentes. Cada grupo foi submetido a uma técnica diferente: Grupo 1 - técnica manual convencional com limas Kerr; Grupo 2 - técnica manual com limas de níquel-titânio; Grupo 3 - Técnica rotatória de níquel-titânio. Os canais radiculares foram preenchidos com pasta de hidróxido de cálcio. Aspectos como homogeneidade das obturações, conicidade e fluidez das preparações foram considerados comparativamente para avaliar a qualidade das instrumentações. Os testes não paramétricos de Kruskal-Wallis e Mann-Whitney foram utilizados para comparar a eficiência do preparo do canal radicular considerando os tempos de preparo dos mesmos. Resultados: Todas as três técnicas foram igualmente eficazes na modelagem dos canais radiculares. O tempo de trabalho foi menor com o instrumento rotatório (p=0,000). Nas comparações pareadas, o tempo de trabalho foi menor com o instrumento rotatório em comparação com as técnicas manuais com limas de aço (p=0,000) e limas de NiTi (p=0,000). Comparando as técnicas manuais, o tempo de trabalho foi menor com as limas de NiTi em comparação com as limas de aço (p=0,011). Além disso, foi encontrada menor variabilidade no tempo de preparo quando o instrumento rotatório foi utilizado. Conclusão: O método rotatório levou um menor tempo operatório em comparação com as técnicas manuais e, portanto, é uma opção preferível para o preparo dos canais radiculares de dentes decíduos.
