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1.
J Invest Dermatol ; 127(11): 2605-11, 2007 Nov.
Article in English | MEDLINE | ID: mdl-17611576

ABSTRACT

Bullous pemphigoid (BP) is an autoimmune disease characterized by subepidermal blistering. Based on previous work, IgG autoantibodies directed against BP180 are thought to be the primary pathogenic agent in BP. In addition to these IgG autoantibodies, however, most BP patients produce IgE class autoantibodies that also react with BP180, and total IgE levels are often elevated in this disease. To directly test whether BP IgE is pathogenic, 6 ng of total IgE isolated from two BP and two normal sera were injected into human skin grafted onto athymic, nude mice. Twenty-four hours after injection, erythematous, elevated plaques were observed in all human skin grafts receiving BP IgE (n=11), but not control IgE (n=9). Histologic and ultrastructural examination of the lesions showed engorgement of blood vessels and a dermal infiltrate composed of neutrophils, eosinophils, and mast cells, many of which were degranulated. At a higher dose of BP IgE (47 ng), histological separation of the epidermis from the dermis was observed in two of the three grafts. The BP IgE-induced erythematous plaques were reminiscent of those clinically seen in BP. This provides early evidence of a direct demonstration of a pathogenic role for IgE class autoantibodies in a human autoimmune disease.


Subject(s)
Autoimmunity/physiology , Immunoglobulin E/immunology , Pemphigoid, Bullous/immunology , Pemphigoid, Bullous/pathology , Skin/pathology , Animals , Autoantibodies/immunology , Female , Humans , Immunoglobulin G/physiology , Mast Cells/immunology , Mast Cells/pathology , Mice , Mice, Nude , Skin/immunology , Skin Transplantation/immunology , Skin Transplantation/pathology
2.
Biochem Biophys Res Commun ; 350(4): 1032-7, 2006 Dec 01.
Article in English | MEDLINE | ID: mdl-17045967

ABSTRACT

Collagen XVII/BP180 is a transmembrane constituent of the epidermal anchoring complex. To study the role of its non-collagenous linker domain, NC16A, in protein assembly and stability, we analyzed the following recombinant proteins: the collagen XVII extracellular domain with or without NC16A, and a pair of truncated proteins comprising the COL15-NC15 stretch expressed with or without NC16A. All four proteins were found to exist as stable collagen triple helices; however, the two missing NC16A exhibited melting temperatures significantly lower than their NC16A-containing counterparts. Protein refolding experiments revealed that the rate of triple helix assembly of the collagen model peptide GPP(10) is greatly increased by the addition of an upstream NC16A domain. In summary, the NC16A linker domain of collagen XVII exhibits a positive effect on both the rate of assembly and the stability of the adjoining collagen structure.


Subject(s)
Autoantigens/chemistry , Non-Fibrillar Collagens/chemistry , Binding Sites , Protein Binding , Protein Conformation , Protein Structure, Tertiary , Collagen Type XVII
4.
J Invest Dermatol ; 125(3): 467-72, 2005 Sep.
Article in English | MEDLINE | ID: mdl-16117787

ABSTRACT

Bullous pemphigoid (BP) is a subepidermal blistering disease characterized by autoantibodies against the hemidesmosomal protein BP180 (BPAg2, type XVII collagen). NC16A, a non-collagenous stretch of the BP180 ectodomain, is the primary target of pathogenic immunoglobulin (Ig)G autoantibodies and IgE class autoantibodies. This study further characterized the IgE-reactive regions of BP180. Of the ten sera from untreated BP patients, eight contained IgE reactive with the entire BP180 ectodomain. The IgE in four of these eight sera reacted with NC16A, whereas in the remaining four sera IgE immunoreactivity was restricted to sites downstream of NC16A. In contrast, IgG reactivity to NC16A was detected in nine of the ten BP sera, and in the remaining serum, IgG, as well as IgE, reacted exclusively with non-NC16A sites on the BP180 ectodomain. Fine mapping of the antigenic sites within NC16A revealed very similar reactivity patterns for IgE and IgG, with NC16A subregion-2 being the major site recognized by both isotypes. Eight of the untreated BP patients were tested for histamine release from their basophils in response to NC16A. Antigen-specific histamine release was observed only in those patients with detectable circulating IgE directed against NC16A (three of eight). Future studies will investigate the pathogenic relevance of anti-BP180 IgE.


Subject(s)
Autoantibodies/immunology , Autoantigens/chemistry , Immunodominant Epitopes/chemistry , Immunoglobulin E/immunology , Pemphigoid, Bullous/immunology , Antibody Specificity , Autoantigens/immunology , Basophils/drug effects , Binding Sites, Antibody , Epitope Mapping , Histamine/metabolism , Humans , Immunodominant Epitopes/immunology , Immunodominant Epitopes/pharmacology , Non-Fibrillar Collagens , Pemphigoid, Bullous/blood , Protein Structure, Tertiary , Collagen Type XVII
5.
J Invest Dermatol ; 120(5): 784-8, 2003 May.
Article in English | MEDLINE | ID: mdl-12713582

ABSTRACT

Bullous pemphigoid (BP) is an autoimmune skin disease characterized by autoantibodies against the hemidesmosomal protein BP180. In addition to IgG autoantibodies, IgE class autoantibodies have been reported in BP patients. Because animal models utilizing only IgG antibodies do not totally replicate human BP, we examined the specificity and potential relevance of IgE autoantibodies in this disease. Thirty BP patients participated in these studies. Serum IgE was measured and the IgE specificity was determined by immunoblotting. Double labeling Immunofluorescence was performed using combinations of specific antibodies to human mast cell tryptase, IgE and BP180. BP180-stimulated histamine release was measured from basophils of untreated BP patients (n=9), BP patients undergoing immunosuppressive therapy (n=9) and controls (n=16). Elevated IgE levels were found In 70% of untreated BP patients. IgE autoantibodies directed against BP180 were detected in 86% of untreated patients and in all but one of these patients the IgE reacted with the NC16A domain of BP180. IgE-coated mast cells were detected in perilesional skin of the BP patients. Moreover, BP180 peptides were detected on these mast cells. BP180-stimulated histamine release was significantly higher in basophils obtained from untreated BP patients compared with control basophils (p=0.006) or from treated BP patients (p=0.01). These findings support the hypothesis that IgE autoantibodies are involved in the pathogenesis of BP. IgE and IgG BP autoantibodies share the same antigenic specificity. Antigen-specific degranulation of basophils and/or mast cells from BP patients suggests a mechanism by which IgE may contribute to lesion development.


Subject(s)
Autoantibodies/chemistry , Carrier Proteins , Cytoskeletal Proteins , Immunoglobulin E/immunology , Nerve Tissue Proteins , Non-Fibrillar Collagens , Pemphigoid, Bullous/immunology , Autoantigens/metabolism , Basophils/metabolism , Collagen/metabolism , Dystonin , Histamine/metabolism , Humans , Immunoblotting , Immunoglobulin E/blood , Microscopy, Fluorescence , Protein Structure, Tertiary , Recombinant Fusion Proteins/metabolism , Collagen Type XVII
6.
Clin Immunol ; 102(3): 310-9, 2002 Mar.
Article in English | MEDLINE | ID: mdl-11890718

ABSTRACT

Linear IgA bullous disease (LABD) is an autoimmune skin disease characterized by subepidermal blisters and IgA autoantibodies directed against the epidermal basement membrane zone (BMZ) of the skin. Various antigens have been identified as targets of IgA autoantibodies including BP180, a type II glycoprotein that spans the BMZ and lamina lucida. Previously, we have identified a subset of LABD patients whose sera contained IgA antibodies against the 16th noncollagenous (NC16A) domain of BP180. NC16A was previously shown to harbor epitopes that are recognized by both autoantibodies and T cells from patients with bullous pemphigoid and herpes gestationis and is thought to be associated with the development of these immunobullous diseases. The aim of this study was to determine whether T lymphocytes from LABD patients with anti-NC16A IgA autoantibodies respond to epitopes in the same region of the BP180 protein. Indeed, of the four LABD patients in our study, all had T cells that specifically proliferated in response to NC16A. Moreover, two subfragments of NC16A were identified as the predominant targets of LABD T cells. Further analysis of T cell lines and clones derived from these patients revealed that these cells express a CD4 memory T cell phenotype and secrete a Th1/Th2 mixed-cytokine profile, characteristics similar to those of T cells in bullous pemphigoid patients. Our data suggest that the BP180 protein, typically the NC16A region, is the common target of both cellular and humoral immune responses in some LABD patients. This information helps to further elucidate the autoimmune mechanisms in this disease.


Subject(s)
Autoantibodies/blood , Autoantigens/immunology , Immunoglobulin A/blood , Skin Diseases, Vesiculobullous/immunology , T-Lymphocytes/immunology , Antigens, CD/metabolism , CD4-Positive T-Lymphocytes/immunology , CD8-Positive T-Lymphocytes/immunology , Cadherins/immunology , Case-Control Studies , Cell Division , Desmoglein 1 , Desmoglein 3 , Humans , Immunoblotting , Monocytes/immunology , Non-Fibrillar Collagens , Collagen Type XVII
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