Reconfigurable Growth of Engineered Living Materials.

The growth of multicellular organisms is a process akin to additive manufacturing where cellular proliferation and mechanical boundary conditions, among other factors, drive morphogenesis. Engineers have limited ability to engineer morphogenesis to manufacture goods or to reconfigure materials comprised of biomass. Herein, a method that uses biological processes to grow and regrow magnetic engineered living materials (mELMs) into desired geometries is reported. These composites contain Saccharomyces cerevisiae and magnetic particles within a hydrogel matrix. The reconfigurable manufacturing process relies on the growth of living cells, magnetic forces, and elastic recovery of the hydrogel. The mELM then adopts a form in an external magnetic field. Yeast within the material proliferates, resulting in 259 ± 14% volume expansion. Yeast proliferation fixes the magnetic deformation, even when the magnetic field is removed. The shape fixity can be up to 99.3 ± 0.3%. The grown mELM can recover up to 73.9 ± 1.9% of the original form by removing yeast cell walls. The directed growth and recovery process can be repeated at least five times. This work enables ELMs to be processed and reprocessed into user-defined geometries without external material deposition.

Growth of Colloidal Nanoplate Liquid Crystals Using Temperature Gradients.

Controlling colloidal self-assemblies using external forces is essential to develop modern electro-optical and biomedical devices. Importantly, shape anisotropic colloids can provide optical properties such as birefringence. Here we demonstrate that external temperature gradients can be effective in controlling nematic liquid crystalline (LC) order in suspensions of plate-like colloids also known as nanoplates. Nanoplates, in an isotropic suspension, wherein their orientations are random, could be effectively moved using a temperature gradient environment causing a phase transition to LC nematic phase. Such controllably formed nematic phase featured large nematic monodomains and enabled topologically more stable structures that were evident from the absence of hedgehog-type defects which are typically found in nematics formed spontaneously via nucleation and growth mechanism in a sufficiently high concentration suspension of nanoplates. Due to their high surface area-to-volume ratio and excellent thermophoretic properties, nanoplates can prove to be ideal candidates for transport of biomolecules through temperature varying environments.