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1.
Theriogenology ; 223: 47-52, 2024 Jul 15.
Article in English | MEDLINE | ID: mdl-38669841

ABSTRACT

This retrospective study aimed at identifying factors that contribute to the success of equine in vitro embryo production by intracytoplasmic sperm injection (ICSI). A total of 7993 ovum pick-up (OPU) sessions were performed, totaling 2540 donor mares and semen from 396 stallions. Oocytes were aspirated at multiple sites in Brazil and were sent to the laboratory, within 6 h from OPU, in pre-maturation medium where they were in vitro matured (IVM) followed by ICSI and in vitro embryo culture for 7-8 days. The number of recovered oocytes, matured oocytes, cleaved embryos and blastocysts were used to explore the effect of age and breed of the donor mare, time of year in which the mare was aspirated and phase of the estrous cycle on the day of follicular aspiration. Mares between 6 and 15 years old were superior to other age groups in most parameters evaluated, including the average number of blastocysts per OPU. The impact of age was similar when evaluated within two breeds, American Quarter Horse (AQHA) and Warmblood mares. We observed that breed (AQHA, Warmblood, Crioulo, Lusitano and Mangalarga) had an important effect on most of the parameter evaluated, including number of oocytes recovered, blastocysts produced per OPU, and blastocyst rates. The overall impact of season was less pronounced than age and breed, with the only statistically significant difference being a higher rate of oocyte maturation during the summer season. Finally, most of the parameters evaluated were superior in follicular phase mares, with or without dominant follicle than luteal phase mares. In conclusion, this retrospective study revealed that breed, age, season and stage of estrous at the time of OPU are all important parameters for the success of equine embryo production by ICSI. This technology enables producing embryos all-year-round from mares of different breeds and ages from OPU-derived oocytes collected at multiple sites.


Subject(s)
Estrous Cycle , Seasons , Sperm Injections, Intracytoplasmic , Animals , Horses/physiology , Horses/embryology , Sperm Injections, Intracytoplasmic/veterinary , Sperm Injections, Intracytoplasmic/methods , Female , Estrous Cycle/physiology , Retrospective Studies , Embryo Culture Techniques/veterinary , Male , Aging/physiology , Age Factors , Oocyte Retrieval/veterinary , Oocyte Retrieval/methods
2.
Psicol. ciênc. prof ; 37(1): 236-247, jan.-mar. 2017.
Article in Portuguese | LILACS | ID: biblio-842129

ABSTRACT

Resumo A Defensoria Pública ocupa lugar em processos judiciais de destituição do poder familiar atuando majoritariamente em favor dos pais de famílias pobres. O psicólogo na Defensoria que atua em processos judiciais é chamado de assistente técnico. O objetivo do presente artigo é discutir concepções pertinentes à atuação do psicólogo na Defensoria a partir do lugar processual do assistente técnico, especialmente compreendido dentro das políticas públicas que trabalham com famílias necessitadas nos termos da lei. Para tanto, foi feito um estudo de caso com pesquisa documental qualitativa em busca das ações radicais possíveis. Os dados e as ações foram pensados à luz das teorias winnicottianas do desenvolvimento e do conceito de juízes anexos, de Foucault. O discurso dominante na Psicologia mantém a lógica de legislações anteriores ao Estatuto da Criança e do Adolescente, em que se culpabiliza a família pobre em processos de destituição do poder familiar. O assistente técnico figurou como importante ator para a inserção, no sistema de justiça, da compreensão de que um bebê não é um bebê sozinho, mas será sempre acompanhado de seus principais cuidadores, pessoas de quem ele depende e que são sujeitos que pretendem desfrutar de uma sociedade livre. A prática comunitária produziu o efeito de criar soluções para a situação que gerou sofrimento aos membros de uma família. Concluímos que teorias que associam pobreza e incapacidade de cuidar dos filhos funcionam de modo similar aos códigos minoristas anteriores ao Estatuto da Criança e do Adolescente...(AU)


Abstract The Public Defender takes place in lawsuits of destitution of the family power acting largely in favor of the parents of poor families. The psychologist at Public Defender that acts in legal proceedings is called technical assistant. The purpose of this article is to discuss pertinent concepts to the psychologist’s activities in Public Defender from the procedural place of the technical assistant, especially understood within the public policies that work with needy families in accordance with law. To that end, we made a case study with qualitative documental research searching for possible radical actions. The data and actions were thought in the light of Winnicott’s development theories and the concept of attachments judges, by Foucault. The dominant discourse in psychology maintains the logic of previous legislation to the Statute of Children and Adolescents, in which blames the poor family in the family power dismissal processes. The technical assistant figured as an important actor for the inclusion, in the justice system, of the understanding that a baby is not a baby alone, but will always be accompanied by their primary caregivers, people he depends and which are subject wishing to enjoy a free society. The community practice had the effect of creating solutions to the situation that caused suffering to family members. We conclude that theories linking poverty and inability to take care of the children, they work in a similar way as minorists codes previous to Child and Adolescent Statute....(AU)


Resumen La Defensa Pública tiene lugar en la destitución del poder familiar actuando en gran medida a favor de los padres de familias pobres. El psicólogo de la Defensa Pública que actúa en los procedimientos legales se llama asistente técnico. El propósito de este artículo es discutir los conceptos pertinentes para la accíon del psicólogo en la Defensa Pública desde el lugar de asistente técnico, entendido sobre todo dentro de las políticas públicas que trabajan con las familias necesitadas de conformidad com la ley. A tal efecto, se realizó un estudio de caso con la investigación cualitativa documental en busca de posibles acciones radicales. Los datos y las acciones fueron diseñados a la luz de las teorías de desarrollo de Winnicott y el concepto de los jueces adjuntos, de Foucault. El discurso dominante en psicología mantiene la lógica de la legislación anterior en el Estatuto de los Niños y Adolescentes, en el que culpa a la familia pobre en los procesos de despido de alimentación de la familia. El asistente técnico hay figurado como un actor importante para la inclusión, en el sistema de justicia, de la comprensión que un bebé no es un bebé solo, pero siempre estará acompañado por sus cuidadores primarios, la gente que depende y que están deseando sujetos a disfrutar una sociedad libre. La práctica comunitaria produjo el efecto de crear soluciones a la situación que causó sufrimiento a los familiares. Llegamos a la conclusión de que las teorías que vinculan la pobreza y la incapacidad para cuidar de los niños trabajan de una manera similar a los códigos anteriores minoritas el Estatuto de los Niños y Adolescentes....(AU)


Subject(s)
Humans , Male , Female , Child , Adolescent , Acting Out , Child Advocacy , Family , Judicial Decisions , Public Attorneys , Public Defender Legal Services , Process Assessment, Health Care , Professional Corporations
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