Genetic dissection of agronomic and quality traits based on association mapping and genomic selection approaches in durum wheat grown in Southern Spain.

Climatic conditions affect the growth, development and final crop production. As wheat is of paramount importance as a staple crop in the human diet, there is a growing need to study its abiotic stress adaptation through the performance of key breeding traits. New and complementary approaches, such as genome-wide association studies (GWAS) and genomic selection (GS), are used for the dissection of different agronomic traits. The present study focused on the dissection of agronomic and quality traits of interest (initial agronomic score, yield, gluten index, sedimentation index, specific weight, whole grain protein and yellow colour) assessed in a panel of 179 durum wheat lines (Triticum durum Desf.), grown under rainfed conditions in different Mediterranean environments in Southern Spain (Andalusia). The findings show a total of 37 marker-trait associations (MTAs) which affect phenotype expression for three quality traits (specific weight, gluten and sedimentation indexes). MTAs could be mapped on the A and B durum wheat subgenomes (on chromosomes 1A, 1B, 2A, 2B and 3A) through the recently available bread wheat reference assembly (IWGSC RefSeqv1). Two of the MTAs found for quality traits (gluten index and SDS) corresponded to the known Glu-B1 and Glu-A1 loci, for which candidate genes corresponding to high molecular weight glutenin subunits could be located. The GS prediction ability values obtained from the breeding materials analyzed showed promising results for traits as grain protein content, sedimentation and gluten indexes, which can be used in plant breeding programs.

High-resolution imagery acquired from an unmanned platform to estimate biophysical and geometrical parameters of olive trees under different irrigation regimes.

The experiments were conducted in a fully-productive olive orchard (cv. Frantoio) at the experimental farm of University of Pisa at Venturina (Italy) in 2015 to assess the ability of an unmanned aerial vehicle (UAV) equipped with RGB-NIR cameras to estimate leaf area index (LAI), tree height, canopy diameter and canopy volume of olive trees that were either irrigated or rainfed. Irrigated trees received water 4-5 days a week (1348 m3 ha-1), whereas the rainfed ones received a single irrigation of 19 m3 ha-1 to relieve the extreme stress. The flight altitude was 70 m above ground level (AGL), except for one flight (50 m AGL). The Normalized Difference Vegetation Index (NDVI) was calculated by means of the map algebra technique. Canopy volume, canopy height and diameter were obtained from the digital surface model (DSM) obtained through automatic aerial triangulation, bundle block adjustment and camera calibration methods. The NDVI estimated on the day of the year (DOY) 130 was linearly correlated with both LAI and leaf chlorophyll measured on the same date (R2 = 0.78 and 0.80, respectively). The correlation between the on ground measured canopy volumes and the ones by the UAV-RGB camera techniques yielded an R2 of 0.71-0.86. The monthly canopy volume increment estimated from UAV surveys between (DOY) 130 and 244 was highly correlated with the daily water stress integral of rainfed trees (R2 = 0.99). The effect of water stress on the seasonal pattern of canopy growth was detected by these techniques in correspondence of the maximum level of stress experienced by the rainfed trees. The highest level of accuracy (RMSE = 0.16 m) in canopy height estimation was obtained when the flight altitude was 50 m AGL, yielding an R2 value of 0.87 and an almost 1:1 ratio of measured versus estimated canopy height.

Hotspots in the genomic architecture of field drought responses in wheat as breeding targets.

Wheat can adapt to most agricultural conditions across temperate regions. This success is the result of phenotypic plasticity conferred by a large and complex genome composed of three homoeologous genomes (A, B, and D). Although drought is a major cause of yield and quality loss in wheat, the adaptive mechanisms and gene networks underlying drought responses in the field remain largely unknown. Here, we addressed this by utilizing an interdisciplinary approach involving field water status phenotyping, sampling, and gene expression analyses. Overall, changes at the transcriptional level were reflected in plant spectral traits amenable to field-level physiological measurements, although changes in photosynthesis-related pathways were found likely to be under more complex post-transcriptional control. Examining homoeologous genes with a 1:1:1 relationship across the A, B, and D genomes (triads), we revealed a complex genomic architecture for drought responses under field conditions, involving gene homoeolog specialization, multiple gene clusters, gene families, miRNAs, and transcription factors coordinating these responses. Our results provide a new focus for genomics-assisted breeding of drought-tolerant wheat cultivars.

Balancing crop yield and water productivity tradeoffs in herbaceous and woody crops.

The links between water and crop yield are well known. In agricultural systems, maximum yield and maximum water productivity (WP; yield divided by water use) are not always compatible goals. In water-limited situations, optimal solutions must be reached by finding a compromise between the levels of crop production and WP. The tradeoffs between production and WP are reviewed here and the dominant effects of the environment on WP are examined. Genetic improvement for WP generally has yield tradeoffs, whereas management measures devised to improve WP also enhance yield. It is shown that partial closure of the stomata in response to environmental stimuli has a variable impact on canopy transpiration, depending on the degree of coupling between the canopy and the atmosphere. In contrast to the behaviour of the major herbaceous crops, WP increases in some woody crops in response to water stress, suggesting that biomass and transpiration are not linearly related, and that deficit irrigation should be successful in these species. Avoiding high evaporative demand periods (e.g. through tolerance to low temperatures) is an important option that aims to increase production and WP. A case study is presented for improving sunflower (Helianthus annuus L.) yield and WP in temperate environments.

Reflections on food security under water scarcity.

Forecasts on population growth and economic development indicate that there will be substantial increases in food demand for the forthcoming decades. We focus here on the water requirements of food production, on the issue of whether there would be enough water to produce sufficient food in the future, and we offer options to face this challenge based on recent trends observed in some agricultural systems. Given the competition for water faced by the agricultural sector, and the uncertainties associated with climate change, improving the efficiency of water use in both rain-fed and irrigated systems is the main avenue to face the challenge. In rain-fed agriculture, managing the risk associated with rainfall variability is a promising option to increase productivity. In irrigated systems, a case study on the improvements in water productivity in Andalusia, Spain, is used to illustrate some of the opportunities to make progress. Progress in reducing irrigation water use in recent decades has been substantial, but decreasing the consumptive use of crops is a much more difficult challenge. The need for more research and technology transfer on improving water-limited crop production is highlighted, and emphasis is placed on interdisciplinary approaches to gain the insight needed to achieve new breakthroughs that would help in tackling this complex problem.

The influence of arbuscular mycorrhizal colonization on soil-root hydraulic conductance in Agrostis stolonifera L. under two water regimes.

The hypothesis that mycorrhizal colonization improves the soil-root conductance in plants was experimentally tested in a growth chamber using pot cultures of Agrostis stolonifera L. colonized by Glomus intraradices. Plants were grown in 50-l pots filled with autoclaved sand/silt soil (1:1), with and without the mycorrhizal fungus. Within the mycorrhizal treatment, half of the pots remained well watered, while the other half was subjected to a progressive water deficit. Soil water potential (estimated as plant water potential measured at the end of the dark period), xylem water potential measured at the tiller base, transpiration rate, and soil water content were monitored throughout the experiment. Soil-root hydraulic conductance was estimated as the ratio between the instantaneous transpiration rate and the soil and xylem water potential difference. To obtain cultures with similar nutritional status, the P in the modified Hoagland's nutrient solution was withheld from the inoculated pots and applied only once a month. Even though there were no differences on growth or nutrient status for the mycorrhizal treatments, water transport was enhanced by the inoculum presence. Transpiration rate was maintained at lower xylem water potential values in the presence of mycorrhizae. The analysis of the relationship between soil-root hydraulic resistance and soil water content showed that mycorrhizal colonization increased soil-root hydraulic conductance as the soil dried. For these growing conditions, this effect was ascribed to the range of 6-10%.