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1.
Rev. bras. pesqui. méd. biol ; Braz. j. med. biol. res;45(12): 1112-1118, Dec. 2012. ilus, tab
Article in English | LILACS | ID: lil-659635

ABSTRACT

The role of B cells in the pathogenesis of hepatitis B virus (HBV) infection has not been explored in depth. In the present study, the activation status of B cells from peripheral blood of healthy controls (N = 20) and patients with acute hepatitis B (AHB, N = 15) or chronic hepatitis B (CHB, N = 30) was evaluated by measuring the expression levels of B-cell activation markers CD69 and CD86, using quantitative real-time PCR and flow cytometry. Moreover, the potential mechanism underlying B-cell activation during HBV infection was further investigated by analyzing the expression profile of FCRL1, an intrinsic activation molecule of B cells. An elevation in the levels of B-cell activation markers including CD69 and CD86 was observed in the AHB patients (44.31 ± 9.27, 27.64 ± 9.26%) compared to CHB patients (30.35 ± 11.27, 18.41 ± 6.56%, P < 0.05), which was still higher than healthy controls (12.23 ± 7.84, 8.22 ± 3.43%, P < 0.05). Furthermore, the expression of FCRL1 was found to be similar to B-cell activation markers, which was highest in AHB patients (70.15 ± 17.11%), lowest in healthy donors (36.32 ± 9.98%, P < 0.05) and half-way between these levels in patients with CHB (55.17 ± 12.03%, P < 0.05). The results were positively associated with aberrant B-cell activation. These data suggest that B cells can play a role in HBV infection, and therefore more effort should be devoted to exploring their functions.


Subject(s)
Adult , Female , Humans , Male , B-Lymphocytes/immunology , Hepatitis B/immunology , Lymphocyte Activation/immunology , Membrane Proteins/immunology , B-Lymphocytes/metabolism , Case-Control Studies , Disease Progression , Flow Cytometry , Gene Expression Profiling , Hepatitis B/genetics , Hepatitis B/metabolism , Lymphocyte Activation/genetics , Membrane Proteins/genetics , Membrane Proteins/metabolism , Real-Time Polymerase Chain Reaction , Sequence Analysis, RNA
2.
Braz J Med Biol Res ; 45(12): 1112-8, 2012 Dec.
Article in English | MEDLINE | ID: mdl-22892829

ABSTRACT

The role of B cells in the pathogenesis of hepatitis B virus (HBV) infection has not been explored in depth. In the present study, the activation status of B cells from peripheral blood of healthy controls (N = 20) and patients with acute hepatitis B (AHB, N = 15) or chronic hepatitis B (CHB, N = 30) was evaluated by measuring the expression levels of B-cell activation markers CD69 and CD86, using quantitative real-time PCR and flow cytometry. Moreover, the potential mechanism underlying B-cell activation during HBV infection was further investigated by analyzing the expression profile of FCRL1, an intrinsic activation molecule of B cells. An elevation in the levels of B-cell activation markers including CD69 and CD86 was observed in the AHB patients (44.31 ± 9.27, 27.64 ± 9.26%) compared to CHB patients (30.35 ± 11.27, 18.41 ± 6.56%, P < 0.05), which was still higher than healthy controls (12.23 ± 7.84, 8.22 ± 3.43%, P < 0.05). Furthermore, the expression of FCRL1 was found to be similar to B-cell activation markers, which was highest in AHB patients (70.15 ± 17.11%), lowest in healthy donors (36.32 ± 9.98%, P < 0.05) and half-way between these levels in patients with CHB (55.17 ± 12.03%, P < 0.05). The results were positively associated with aberrant B-cell activation. These data suggest that B cells can play a role in HBV infection, and therefore more effort should be devoted to exploring their functions.


Subject(s)
B-Lymphocytes/immunology , Hepatitis B/immunology , Lymphocyte Activation/immunology , Membrane Proteins/immunology , Adult , B-Lymphocytes/metabolism , Case-Control Studies , Disease Progression , Female , Flow Cytometry , Gene Expression Profiling , Hepatitis B/genetics , Hepatitis B/metabolism , Humans , Lymphocyte Activation/genetics , Male , Membrane Proteins/genetics , Membrane Proteins/metabolism , Real-Time Polymerase Chain Reaction , Sequence Analysis, RNA
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