ABSTRACT
OBJECTIVE: To assess whether hyperinoculation of cats with a feline herpesvirus-1, calicivirus, and panleukopenia virus (FVRCP) vaccine could be used as a model to study interstitial nephritis and to assess humoral and cell-mediated immune responses toward vaccinal α-enolase. ANIMALS: 6 healthy young adult purpose-bred research cats. PROCEDURES: Baseline renal cortical biopsies, whole blood, serum, and urine were collected prior to administration of a commercial FVRCP parenteral vaccine. Vaccine hyperinoculation was defined as a total of 8 vaccinations given at 2-week intervals over a 14-week period. Blood samples were collected immediately prior to each vaccination, and a second renal biopsy was performed 2 weeks after hyperinoculation (week 16). Renal histopathology, renal α-enolase immunohistochemistry, and assays to detect humoral and cell-mediated immune reactions against Crandell-Rees feline kidney (CRFK) cell lysates and α-enolase were performed. An α-enolase immunoreactivity score for renal tubules and glomeruli based on signal intensity was determined by a blinded pathologist. RESULTS: Hyperinoculation with the vaccine was not associated with clinicopathologic evidence of renal dysfunction, and interstitial nephritis was not recognized by light microscopy in the time studied. The mean serum absorbance values for antibodies against CRFK antigen and α-enolase were significantly (P < 0.001) higher at weeks 4, 8, and 16 versus week 0. Renal tubular and glomerular α-enolase immunoreactivity scores were higher at week 16 compared to baseline. CLINICAL RELEVANCE: Findings suggested that systemic immunological reactions occurred and renal tissues were affected by vaccine hyperinoculation; however, short-term FVRCP vaccine hyperinoculation cannot be used to study interstitial nephritis in cats.
Subject(s)
Calicivirus, Feline , Cat Diseases , Herpesviridae , Viral Vaccines , Animals , Antibodies, Viral , Cat Diseases/pathology , Cat Diseases/prevention & control , Cats , Feline Panleukopenia Virus , Kidney , Phosphopyruvate Hydratase , VaricellovirusABSTRACT
OBJECTIVES: The aim of this study was to evaluate the blood of cats in Colorado, USA, with suspected infectious causes of anemia for the presence of Babesia species and Cytauxzoon felis DNA. Results of PCR testing for other common vector-borne diseases potentially associated with anemia are also reported. METHODS: Samples from 101 cats were tested using a PCR assay that coamplified the DNA of C felis and Babesia species mitochondrial DNA. PCR testing for DNA of hemoplasmas, Bartonella species, Ehrlichia species, Anaplasma species, Neorickettsia risticii and Wolbachia genera was also performed if not carried out previously. RESULTS: Twenty-two cats (21.8%) were positive for DNA of an infectious agent. DNA from hemoplasma species were amplified from 14 cats (13.9%). Bartonella species DNA was amplified from four cats (4%) and Ehrlichia canis, Anaplasma platys, Anaplasma phagocytophilum and Wolbachia genera DNA were amplified from one cat each. Babesia species and C felis mitochondrial DNA were not amplified from any sample. CONCLUSIONS AND RELEVANCE: Based on the results of this study, it does not appear that Babesia species or C felis are clinically relevant in anemic cats in Colorado, USA. For C felis, this suggests that the vector Amblyomma americanum is still uncommon in this geographic area.
ABSTRACT
BACKGROUND: Juvenile onset generalized demodicosis (JOGD) is thought to occur due to immunological abnormalities and is over-represented in pit bull terrier-type dogs. ANIMALS: Twelve pit bull terrier-type dogs with JOGD and 12 age-matched healthy pit bull terrier-type dogs. OBJECTIVE: To investigate immunological differences between age-matched healthy and JOGD pit bull terrier-type dogs by flow cytometry, multiplex, molecular and serological assays. METHODS AND MATERIALS: Flow cytometry quantified B cells expressing MHCII or surface-bound IgG, CD4+ T cells expressing MHCII, CD8 T cells expressing MHCII or CD11a, neutrophil and monocyte markers. Surface expression was quantified by calculating the geometric mean fluorescence index. The Wilcoxon rank sum test was used to compare median results for IL-2, IL-4, IL-6, IL-7, IL-8, IL-10, IL-13, IL-18, FOXP3, monocyte chemoattractant protein-1, GM-CSF, KC, IgE, IgA, IgG, IgM, C-reactive protein, lymphocyte, neutrophil and monocyte in the groups. IFN-gamma, IP-10, IL-15, IL-31 and TNF-alpha also were measured; however, insufficient dogs (<5) had values that were in range of the assay to allow for statistical evaluation. Significance was defined as P < 0.05. RESULTS: Serum concentrations of IL-2, IL-18 and MCP-1 were significantly higher (P = 0.01, P = 0.01, P = 0.04) in the JOGD group. Also, IgA median value was significantly higher (P = 0.002) in pit bull terrier-type dogs with JOGD. Flow cytometry revealed that T-cell, neutrophil and monocyte markers were not different between groups. CONCLUSIONS: Results suggest an appropriate compensatory immune response by pit bull terrier-type dogs in the JOGD group and do not support the explanation of global immune deficiency in these dogs.
Subject(s)
Dog Diseases/parasitology , Mite Infestations/veterinary , Age Factors , Animals , B-Lymphocytes/immunology , CD4-Positive T-Lymphocytes/immunology , Case-Control Studies , Chemokine CCL2/blood , Dog Diseases/immunology , Dogs , Female , Flow Cytometry/veterinary , Interleukins/blood , Male , Mite Infestations/immunology , Mite Infestations/parasitology , Mites/immunologyABSTRACT
BACKGROUND: Hemoplasma species (spp.) commonly cause infections in cats worldwide. However, data on risk factors for infections are limited. The aim of this study was to determine the prevalence of hemoplasma spp. infections in cats in Southern Germany and to assess risk factors associated with infection. RESULTS: DNA was extracted from blood samples of 479 cats presented to different veterinary hospitals for various reasons. DNA of feline hemoplasmas was amplified by use of a previously reported PCR assay. Direct sequencing was used to confirm all purified amplicons and compared to hemoplasma sequences reported in GenBank. Results were evaluated in relation to the age, sex, housing conditions, feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV) status of the cats. The overall hemoplasma prevalence rate was 9.4% (45/479; 95% CI: 7.08-12.36). 'Candidatus Mycoplasma (M.) haemominutum' (Mhm) DNA was amplified from 42 samples, M. haemofelis from 2, and M. haemocanis from 1 sample. There was a significantly higher risk of hemoplasma infection in cats from multi-cat households, in outdoor cats, as well as in cats with FIVinfection and in cats with abortive FeLV infection, but not in cats with progressive or regressive FeLV infection. CONCLUSIONS: Mhm infection is common in cats in Southern Germany. Higher prevalence in multi-cat households and associations with FeLV infection likely reflect the potential for direct transmission amongst cats. Outdoor access, male gender, and FIV infection are additional risk factors that might relate to aggressive interactions and exposure to vectors.
Subject(s)
Cat Diseases/microbiology , DNA, Bacterial/genetics , Mycoplasma Infections/veterinary , Mycoplasma/classification , Animals , Cat Diseases/epidemiology , Cats , DNA, Bacterial/blood , DNA, Bacterial/isolation & purification , Female , Germany/epidemiology , Male , Mycoplasma/genetics , Mycoplasma/isolation & purification , Mycoplasma Infections/epidemiology , Mycoplasma Infections/microbiology , Polymerase Chain Reaction/veterinary , Prevalence , Risk FactorsABSTRACT
Objectives The objective of this study was to evaluate wild-caught mosquitoes for evidence of hemotropic Mycoplasma species DNA and to determine whether the feline hemoplasmas, Mycoplasma haemofelis (Mhf) and ' Candidatus Mycoplasma haemominutum' (Mhm), can be transmitted by Aedes aegypti mosquitoes in a laboratory setting. Methods Wild-caught mosquito pools (50 mosquitoes per pool, 84 pools) utilized in routine public health department disease surveillance programs were tested for hemotropic Mycoplasma species DNA using PCR with primers designed to amplify all known hemoplasmas. Additionally, mosquitoes were trapped in the vicinity of known feral cat colonies, pooled (50 mosquitoes per pool) and tested (84 pools). Purpose-bred cats housed in a research facility were infected with Mhf or Mhm and then colonized laboratory A aegypti were fed upon the bacteremic cats. After a 7 day incubation period, mosquitoes previously fed on infected cats were allowed to feed again on naive cats, which were monitored for bacteremia for 10 weeks. Results Mycoplasma wenyonii DNA was confirmed in one wild-caught mosquito pool by DNA sequencing. While 7% of cats tested in feral colonies were hemoplasma positive, none of the mosquitoes trapped near colonies were positive. Hemoplasma DNA was amplified from A aegypti by PCR immediately after the infectious blood meal, but DNA was not detected at 7 and 14 days after feeding. Although evidence for uptake of organisms existed, hemoplasma DNA was not amplified from the experimentally infested cats in the 10 week observation period. Conclusions and relevance While wild-caught mosquitoes contained hemoplasma DNA and laboratory reared A aegypti mosquitoes take up hemoplasmas during the blood meal, there was no evidence of biologic transmission in this model.
Subject(s)
Aedes/microbiology , Bacteremia/veterinary , Cat Diseases/transmission , Mosquito Vectors/microbiology , Mycoplasma Infections/veterinary , Mycoplasma/classification , Animals , Bacteremia/transmission , Cats , DNA, Bacterial/analysis , Female , Male , Mycoplasma/genetics , Mycoplasma Infections/transmission , Polymerase Chain Reaction/veterinary , Specific Pathogen-Free OrganismsABSTRACT
Objectives The objective was to investigate the effect of one dose of an inactivated feline herpesvirus-1 (FHV-1), feline calicivirus (FCV) and panleukopenia virus (FPV) vaccine (FVRCP) or one dose of a modified live (ML) FVRCP vaccine on clinical signs and shedding of FHV-1 in specific pathogen-free kittens after challenge with FHV-1 7 days after vaccination. Methods Twenty-four FHV-1 seronegative 5-month-old kittens were randomized into three groups of eight kittens. Group 1 kittens were maintained as unvaccinated controls, group 2 kittens were administered one dose of the inactivated FVRCP vaccine subcutaneously (SC) and group 3 kittens were administered one dose of the ML FVRCP vaccine SC. All 24 cats were administered FHV-1 by nasal and oropharyngeal inoculation 7 days later and were observed daily for clinical signs of illness for 21 days. Results In the 21 days after FHV-1 challenge, both groups of vaccinated cats were less likely to be clinically ill (indicated by lower cumulative clinical scores) than control cats ( P <0.001). There was no statistical difference in total clinical score between the two vaccinated groups ( P = 0.97). Although the total clinical score was similar between both vaccines, signs of respiratory disease were significantly fewer in the kittens vaccinated with the inactivated FVRCP vaccine compared with the ML FVRCP vaccine ( P = 0.005) during the period after inoculation when the majority of clinical disease was observed. Conclusions and relevance Parenteral administration of either the inactivated FVRCP vaccine or the ML FVRCP vaccine can decrease clinical signs of illness due to FHV-1 on a day 7 challenge when compared with controls. Use of either vaccine product is indicated in cats at risk of acute exposure to FHV-1.
Subject(s)
Cat Diseases/prevention & control , Herpesviridae Infections/veterinary , Herpesviridae/immunology , Viral Vaccines , Animals , Animals, Newborn , Calicivirus, Feline/immunology , Cats , Female , Herpesviridae/physiology , Herpesviridae Infections/prevention & control , Injections, Subcutaneous , Male , Specific Pathogen-Free Organisms , Treatment Outcome , Vaccination/veterinary , Vaccines, Attenuated/administration & dosage , Vaccines, Inactivated/administration & dosage , Viral Vaccines/administration & dosage , Virus SheddingABSTRACT
Clinical signs of upper respiratory tract infection can be hard to manage in cats, particularly those in shelters. In this study, clinical data were collected from chronically ill (3-4 weeks' duration) cats with suspected feline herpesvirus-1 (FHV-1) or feline calicivirus (FCV) infections after administration of one of two novel therapies. Group A cats were administered a commercially available formulation of human interferon-α2b at 10,000 U/kg subcutaneously for 14 days, and group B cats were administered one dose of a FHV-1 and FCV intranasal vaccine. Molecular assays for FHV-1 and FCV were performed on pharyngeal samples, and a number of cytokines were measured in the blood of some cats. A clinical score was determined daily for 14 days, with cats that developed an acceptable response by day 14 returning to the shelter for adoption. Those failing the first treatment protocol were entered into the alternate treatment group. During the first treatment period, 8/13 cats in group A (61.5%) and all 12 cats in group B (100%) had apparent responses. The seven cats positive for nucleic acids of FHV-1 or FCV responded favorably, independent of the treatment group. There were no differences in cytokine levels between cats that responded to therapy or failed therapy. Either protocol assessed here may be beneficial in alleviating chronic clinical signs of suspected feline viral upper respiratory tract disease in some cats that have failed other, more conventional, therapies. The results of this study warrant additional research involving these protocols.
Subject(s)
Antiviral Agents/administration & dosage , Cat Diseases/therapy , Interferon-alpha/administration & dosage , Respiratory Tract Infections/veterinary , Viral Vaccines/administration & dosage , Administration, Intranasal , Animals , Caliciviridae Infections/drug therapy , Caliciviridae Infections/prevention & control , Caliciviridae Infections/veterinary , Calicivirus, Feline , Cat Diseases/drug therapy , Cat Diseases/prevention & control , Cats , Chronic Disease/drug therapy , Chronic Disease/prevention & control , Herpesviridae Infections/drug therapy , Herpesviridae Infections/prevention & control , Herpesviridae Infections/veterinary , Interferon alpha-2 , Recombinant Proteins/administration & dosage , Respiratory Tract Infections/drug therapy , Respiratory Tract Infections/prevention & controlABSTRACT
Prevalence of Anaplasma, Ehrlichia, Neorickettsia, and Wolbachia DNA in blood of 479 cats collected in different veterinary clinics in Southern Germany was determined using a previously published conventional PCR using 16S-23S intergenic spacer primers (5' CTG GGG ACT ACG GTC GCA AGA C 3' - forward; 5' CTC CAG TTT ATC ACT GGA AGT T 3' - reverse). Purified amplicons were sequenced to confirm genus and species. Associations between rickettsial infections, and feline immunodeficiency virus (FIV), as well as feline leukemia virus (FeLV) status were evaluated. Rickettsial prevalence was 0.4% (2/479; CI: 0.01-1.62%). In the two infected cats, Anaplasma phagocytophilum DNA was amplified. These cats came from different environment and had outdoor access. Both were ill with many of their problems likely related to other diseases. However, one cat had neutrophilia with left shift and the other thrombocytopenia potentially caused by their A. phagocytophilum infection. There was no significant difference in the FIV and FeLV status between A. phagocytophilum-negative and -positive cats. A. phagocytophilum can cause infection in cats in Southern Germany, and appropriate tick control is recommended.
Subject(s)
Anaplasmataceae Infections/veterinary , Cat Diseases/epidemiology , Rickettsia Infections/veterinary , Anaplasma/genetics , Anaplasmataceae Infections/epidemiology , Anaplasmataceae Infections/microbiology , Animals , Cat Diseases/microbiology , Cat Diseases/virology , Cats , Ehrlichia/genetics , Germany/epidemiology , Immunodeficiency Virus, Feline/genetics , Lentivirus Infections/epidemiology , Lentivirus Infections/veterinary , Lentivirus Infections/virology , Leukemia Virus, Feline/genetics , Neorickettsia/genetics , Polymerase Chain Reaction/methods , Prevalence , Retroviridae Infections/epidemiology , Retroviridae Infections/veterinary , Retroviridae Infections/virology , Rickettsia Infections/epidemiology , Rickettsia Infections/prevention & control , Thrombocytopenia/microbiology , Thrombocytopenia/veterinary , Ticks/microbiology , Tumor Virus Infections/epidemiology , Tumor Virus Infections/veterinary , Wolbachia/geneticsABSTRACT
BACKGROUND: Bartonella henselae is transmitted amongst cats by Ctenocephalides felis and is associated with multiple clinical syndromes in cats and people. In a previous study, monthly spot-on administration of 10% imidacloprid/1% moxidectin was shown to block transmission of B. henselae amongst cats experimentally exposed to infected C. felis. The purpose of this study was to determine whether application of a flea and tick collar containing 10% imidacloprid and 4.5% flumethrin would lessen C. felis transmission of B. henselae amongst cats for 8 months. METHODS: Specific pathogen free cats (n = 19) were housed in three adjoining enclosures that were separated by mesh to allow C. felis to pass among groups but prevent cats in different enclosures from contacting one another. One group of 4 cats was inoculated intravenously with B. henselae and after infection was confirmed in all cats based on positive PCR assay results, the cats were housed in the middle enclosure. The B. henselae infected cat group was flanked by a group of 8 cats that had the collar placed and maintained for the duration of the study and a group of 7 cats that were not treated. Ctenocephalides felis (50 males and 50 females) raised in an insectary were placed on each of the 4 cats in the B. henselae infected group monthly for 7 applications and then every 2 weeks for 4 applications starting the day the collar was applied. Blood was collected from all cats weekly for Bartonella spp. PCR, serology and culture. RESULTS: While side-effects associated with the collars were not noted, persistent fever necessitating enrofloxacin therapy occurred in two of the untreated cats. While B. henselae infection was ultimately confirmed in 4 of 7 of the untreated cats, none of the cats with collars became infected (P = 0.026). CONCLUSIONS: In this study design, use of a collar containing 10% imidacloprid and 4.5% flumethrin was well tolerated and prevented C. felis transmission of B. henselae amongst cats for 8 months.
Subject(s)
Angiomatosis, Bacillary/veterinary , Cat Diseases/prevention & control , Flea Infestations/prevention & control , Imidazoles/administration & dosage , Insect Control/methods , Insecticides/administration & dosage , Nitro Compounds/administration & dosage , Pyrethrins/administration & dosage , Angiomatosis, Bacillary/prevention & control , Angiomatosis, Bacillary/transmission , Animals , Bartonella henselae/isolation & purification , Cat Diseases/transmission , Cats , Ctenocephalides/growth & development , NeonicotinoidsABSTRACT
OBJECTIVE: To develop a broad-range 28S ribosomal DNA quantitative PCR (qPCR) assay for detection of fungal DNA in equine endometrial samples. SAMPLE: 12 fungal samples from a clinical diagnostic laboratory and 29 samples obtained from 17 mares. PROCEDURES: The qPCR assay was optimized with commercially acquired fungal organisms and validated with samples obtained from the clinical diagnostic laboratory. Subsequently, 29 samples from 17 mares suspected of having fungal endometritis were evaluated via the qPCR assay and via traditional fungal culture and endometrial cytology. Amplicons from the qPCR assay were subjected to genetic sequencing to identify the organisms. RESULTS: The qPCR assay theoretically had a detection threshold of 2 organisms of Candida albicans. Fungal DNA was amplified from all 12 fungal samples from the commercial diagnostic laboratory. Fungal identification by use of genetic sequencing was successful for 34 of 36 amplicons from the 12 samples assayed. A fungal agent was identified via qPCR assay and genetic sequencing in all 12 samples; in contrast, a fungal agent was identified in only 8 of 12 samples via standard fungal culture and biochemical analysis. The qPCR assay detected fungal DNA in samples from 12 of 17 mares suspected of having fungal endometritis. CONCLUSIONS AND CLINICAL RELEVANCE: A rapid, sensitive, and repeatable qPCR assay was developed for detection of fungal DNA from equine endometrial samples. The qPCR may prove to be clinically useful as an adjunct to microbial culture and cytologic examination to provide identification of fungal organisms in a timely manner.
Subject(s)
DNA, Fungal/analysis , Endometriosis/veterinary , Fungi/classification , Fungi/isolation & purification , Horse Diseases/diagnosis , Real-Time Polymerase Chain Reaction/methods , Animals , Aspergillus fumigatus/classification , Aspergillus fumigatus/genetics , Aspergillus fumigatus/isolation & purification , Candida albicans/classification , Candida albicans/genetics , Candida albicans/isolation & purification , DNA, Ribosomal/analysis , Endometriosis/diagnosis , Endometriosis/microbiology , Female , Fungi/genetics , Horse Diseases/microbiology , Horses , Mycoses/diagnosis , Mycoses/microbiology , RNA, Ribosomal, 28S/analysis , Real-Time Polymerase Chain Reaction/veterinaryABSTRACT
In this pilot study, 12 adult, gang-housed cats that were known to be previously exposed (n=12) to feline herpesvirus-1 (FHV-1) and/or vaccinated against (n=2) feline calicivirus (FCV) and FHV-1 were randomly assigned to one of two groups of six cats each. Nasal and pharyngeal samples were collected from each cat on days -7, -3, and 0 prior to vaccination and on days 3, 7, 10, 14, 17, 21, and 28 after vaccination with an FHV-1, FCV, and panleukopenia (FVRCP) vaccine developed for intranasal (six cats) or parenteral (six cats) use. FHV-1 DNA was amplified from 1/12 cats (1/69 samples; 1.4%) prior to vaccination and 2/12 cats after vaccination (2/154 samples; 1.3%). FCV RNA was amplified from 2/12 cats (2/69 samples; 2.9%) prior to vaccination and 7/12 cats (12/154 samples; 7.8%) after vaccination. Positive molecular diagnostic assay results for FHV-1 and FCV were uncommon prior to or after vaccination in these cats.
Subject(s)
Cat Diseases/diagnosis , Cat Diseases/virology , RNA, Viral/analysis , Respiratory Tract Infections/veterinary , Animals , Antibodies, Viral/analysis , Caliciviridae Infections/diagnosis , Caliciviridae Infections/veterinary , Caliciviridae Infections/virology , Calicivirus, Feline/immunology , Cat Diseases/prevention & control , Cats , Feline Panleukopenia/diagnosis , Feline Panleukopenia/prevention & control , Feline Panleukopenia/virology , Feline Panleukopenia Virus/immunology , Female , Herpesviridae Infections/diagnosis , Herpesviridae Infections/veterinary , Herpesviridae Infections/virology , Male , Nasal Mucosa/virology , Pharynx/virology , Pilot Projects , Polymerase Chain Reaction/veterinary , Random Allocation , Respiratory Tract Infections/diagnosis , Respiratory Tract Infections/prevention & control , Respiratory Tract Infections/virology , Varicellovirus/immunology , Viral VaccinesABSTRACT
Benign, inflammatory polyps may affect the nasopharynx and auditory canal of cats. It has been proposed that inflammation induced by infectious disease agents could trigger polyp formation. The objective of this pilot study was to determine the prevalence of feline herpesvirus-1 (FHV-1), feline calicivirus (FCV), Mycoplasma species, Bartonella species and Chlamydophila felis nucleic acids in polyp tissues collected from 30 clinically affected cats. Samples collected from the tympanic bulla from 12 clinically normal cats were also assayed. DNA or RNA of some of the target agents were amplified from samples from 25% of normal cats and 33% of affected cats; however, statistical associations were not detected for individual agent results or grouped results. The study documents that common oropharyngeal or blood borne agents can be detected in the tympanic bullae of normal cats. Failure to consistently amplify RNA or DNA of the select agents from polyp tissues suggests the agents studied were not directly associated with the pathogenesis of this syndrome in the cats tested. Alternately, the inflammatory response may have cleared microbial nucleic acids to undetectable levels by the time of sample collection.
Subject(s)
Cats/microbiology , Ear, Middle/microbiology , Mycoplasma/classification , Nasal Polyps/veterinary , Nasopharynx/microbiology , Animals , Bartonella/classification , Bartonella/isolation & purification , Calicivirus, Feline/isolation & purification , Chlamydophila/isolation & purification , DNA, Viral/analysis , Female , Herpesviridae/isolation & purification , Inflammation/veterinary , Male , Mycoplasma/isolation & purification , Nasal Polyps/microbiology , Nucleic Acid Amplification Techniques/veterinary , Pilot Projects , Prevalence , Prospective Studies , RNA, Viral/analysisABSTRACT
Feline gingivostomatitis (FGS) is a common syndrome in cats; feline calicivirus (FCV), feline herpesvirus 1 (FHV-1), and Bartonella species are common differential diagnoses. In this study, blood from 70 cats with FGS and 61 healthy control cats was tested for Bartonella species antibodies in serum by enzyme-linked immunosorbent assay and Western blot immunoassay and DNA in blood using a conventional polymerase chain reaction assay. Additionally, fresh oral biopsies from cats with FGS (n=42) and 19 healthy controls were tested for FCV RNA, FHV-1 DNA and Bartonella species DNA. The prevalence rates for Bartonella species antibodies and DNA in the blood and the tissues did not differ between the two groups. FHV-1 DNA was also not significantly different between groups. Only FCV RNA was present in significantly more cats with FGS (40.5%) than control cats (0%). The results suggest that FCV was associated with FGS in some of the cats.
Subject(s)
Cat Diseases/microbiology , Gingivitis/veterinary , Stomatitis/veterinary , Animals , Antibodies, Bacterial/blood , Antibodies, Viral/blood , Bartonella/immunology , Bartonella/isolation & purification , Calicivirus, Feline/immunology , Calicivirus, Feline/isolation & purification , Cat Diseases/virology , Cats , Coronavirus, Feline/immunology , Coronavirus, Feline/isolation & purification , DNA, Bacterial/analysis , DNA, Viral/analysis , Enzyme-Linked Immunosorbent Assay/veterinary , Female , Gingivitis/microbiology , Gingivitis/virology , Male , Polymerase Chain Reaction/veterinary , Prospective Studies , Stomatitis/microbiology , Stomatitis/virology , Stomatitis, Herpetic/microbiology , Stomatitis, Herpetic/veterinary , Stomatitis, Herpetic/virologyABSTRACT
Rickettsia species antibodies have been detected in some cats but it is unknown whether infected cats develop clinical signs. The prevalence of Rickettsia species deoxyribonucleic acid (DNA) in blood from clinically ill cats has not been determined. The objective of this study was to determine if cats with fever (body temperature >or=102.5 degrees F [39.2 degrees C]) were more likely to have evidence of rickettsial infection than healthy, age-matched, control cats with a body temperature<102.5 degrees F. Rickettsia species polymerase chain reaction (PCR) assays were performed to detect rickettsial DNA extracted from blood (71 paired samples), indirect immunofluorescence assays (IFA) were performed to detect serum antibodies against Rickettsia felis (90 paired samples) and Rickettsia rickettsii (91 paired samples), and the results between pairs were compared. All samples were negative for Rickettsia species DNA. More cats with fever were seropositive for R felis or R rickettsii than control cats, but results were not statistically significant. Results of this pilot study failed to show an association between Rickettsia species DNA or Rickettsia species antibodies and fever.
Subject(s)
Antibodies, Bacterial/blood , Cat Diseases/blood , DNA, Bacterial/isolation & purification , Fever/veterinary , Rickettsia Infections/veterinary , Rickettsia , Animals , Case-Control Studies , Cats , Female , Fever/blood , Fluorescent Antibody Technique, Indirect/veterinary , Male , Pilot Projects , Polymerase Chain Reaction/veterinary , Rickettsia/immunology , Rickettsia/isolation & purification , Rickettsia Infections/blood , Rickettsia Infections/epidemiology , Rickettsia felis/immunology , Rickettsia felis/isolation & purification , Rickettsia rickettsii/immunology , Rickettsia rickettsii/isolation & purification , Seroepidemiologic StudiesABSTRACT
OBJECTIVE: To use PCR assays to determine the prevalence of feline herpesvirus 1 (FHV-1), Chlamydophila felis, and Mycoplasma spp DNA in conjunctival cells collected from cats with and without conjunctivitis; to compare results of conventional and real-time fluorogenic PCR assays for amplification of FHV-1 DNA; and to determine whether copy numbers of FHV-1 DNA are correlated with conjunctivitis. ANIMALS: 55 cats with active conjunctivitis, 39 healthy cats that never had conjunctivitis, and 32 cats with a history of conjunctivitis that had been resolved for at least 3 months. PROCEDURES: Samples were obtained by rolling cotton-tipped applicators on the ventral conjunctiva of awake cats treated topically with proparacaine. The DNA was extracted from the swab specimens and assessed in PCR assays to detect DNA of FHV-1 (fluorogenic PCR assay and conventional PCR assay), Mycoplasma spp (conventional PCR assay), and C felis (conventional PCR assay). RESULTS: Overall prevalence rates of FHV-1, C felis, and Mycoplasma spp as assessed by the conventional PCR assays were 6.7%, 3.2%, and 9.6%, respectively. Percentage concordance between conventional PCR and fluorogenic PCR assays for FHV-1 was 92.5%. There were no significant differences among the 3 groups of cats for the mean copy number of FHV-1 divided by the copy number of glyceraldehyde-3-phosphate dehydrogenase. CONCLUSIONS AND CLINICAL RELEVANCE: Mycoplasma spp were the most prevalent organism detected and was associated with conjunctivitis. This study could not confirm that there are increased copy numbers of FHV-1 DNA in cats with conjunctivitis, compared with the copy numbers for cats without conjunctivitis.
Subject(s)
Cat Diseases/microbiology , Chlamydophila/isolation & purification , Conjunctiva/microbiology , Conjunctivitis/veterinary , DNA, Viral/analysis , Herpesviridae/isolation & purification , Mycoplasma/isolation & purification , Animals , Cat Diseases/epidemiology , Cats , Chlamydophila/genetics , Chlamydophila Infections/epidemiology , Chlamydophila Infections/immunology , Chlamydophila Infections/microbiology , Chlamydophila Infections/veterinary , Conjunctiva/cytology , Conjunctivitis/epidemiology , Conjunctivitis/microbiology , DNA, Bacterial/isolation & purification , DNA, Viral/genetics , Herpesviridae/genetics , Herpesviridae Infections/epidemiology , Herpesviridae Infections/veterinary , Herpesviridae Infections/virology , Mycoplasma/genetics , Mycoplasma Infections/epidemiology , Mycoplasma Infections/microbiology , Mycoplasma Infections/veterinary , Polymerase Chain Reaction/veterinaryABSTRACT
Rickettsia felis is associated with fever, headache, myalgia, and macular rash in some infected humans and has been detected in the cat flea (Ctenocephalides felis) in many countries around the world. While some naturally exposed cats have been assessed for antibodies against R felis, to our knowledge, no one has reported use of polymerase chain reaction (PCR) to attempt to amplify R felis DNA from client-owned cats and the fleas collected from them. In this study, we assayed 92 pairs of cat blood and flea extracts from Alabama, Maryland and Texas, using PCR assays that amplify a region of the citrate synthase gene (gltA) and the outer membrane protein B gene (ompB). Of the 92 pairs, 62 of 92 (67.4%) flea extracts and none of the cat blood samples were positive for R felis DNA.
Subject(s)
Cat Diseases/microbiology , Cats/blood , Rickettsia Infections/epidemiology , Rickettsia Infections/veterinary , Siphonaptera/microbiology , Alabama/epidemiology , Animals , Cat Diseases/blood , Cat Diseases/epidemiology , Cat Diseases/parasitology , Cats/microbiology , Female , Male , Maryland/epidemiology , Polymerase Chain Reaction/veterinary , Prevalence , Rickettsia Infections/blood , Rickettsia felis/classification , Rickettsia felis/genetics , Rickettsia felis/isolation & purification , Texas/epidemiologyABSTRACT
OBJECTIVE: To determine whether Ctenocephalides felis can transmit Mycoplasma haemofelis (Mhf) and Candidatus Mycoplasma haemominutum (Mhm) through hematophagous activity between cats. ANIMALS: 11 cats. PROCEDURE: 2 cats were carriers of either Mhf or Mhm. Nine cats had negative results via polymerase chain reaction (PCR) assay for Mhf and Mhm DNA; 3 of those cats were infected from the chronic carriers via i.v. inoculation of blood. At the time of maximum organism count for each of the Mycoplasma spp, 1 chamber containing 100 C felis was bandaged to the amplifier cats. Five days later, fleas, feces, larvae, or eggs from each chamber were analyzed for Mycoplasma spp DNA. Viable fleas from the chambers were allocated into new chambers (3 Mhm and 6 Mhf) and attached to naïve cats for 5 days. Cats were monitored daily for clinical signs and weekly via CBC and PCR assay for infection with Mhf or Mhm for a minimum of 8 weeks. RESULTS: Uptake of Mhf and Mhm DNA into fleas, feces, and, potentially, eggs and larvae was detected. Of the naïve cats fed on by Mhf-infected fleas, 1 cat transiently yielded positive PCR assay results for Mhf on 1 sampling date without clinical or hematologic changes consistent with Mhf infection. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that hematophagous transfer of Mhm and Mhf into fleas occurred and that C felis is a possible vector for Mhf via hematophagous activity.
Subject(s)
Cat Diseases/microbiology , Cat Diseases/transmission , Insect Vectors/microbiology , Mycoplasma Infections/veterinary , Mycoplasma/genetics , Siphonaptera/microbiology , Animals , Cats , Feces/chemistry , Mycoplasma Infections/microbiology , Mycoplasma Infections/transmission , Polymerase Chain Reaction/veterinary , Time FactorsABSTRACT
OBJECTIVE: To determine whether administration of Crandell-Rees feline kidney (CRFK) cell lysates or vaccines against feline viral rhinotracheitis, calicivirus, and panleukopenia (FVRCP vaccines) that likely contain CRFK cell proteins induces antibodies against CRFK cell or feline renal cell (FRC) lysates in cats. ANIMALS: 14 eight-week-old cats. PROCEDURE: Before and after the study, renal biopsy specimens were obtained from each cat for histologic evaluation. Each of 4 FVRCP vaccines was administered to 2 cats at weeks 0, 3, 6, and 50. Between weeks 0 and 50, another 3 pairs of cats received 11 CRFK cell lysate inoculations SC (10, 50, or 50 microg mixed with alum). Clinicopathologic evaluations and ELISAs to detect serum antibodies against CRFK cell or FRC lysates were performed at intervals. RESULTS: Cats had no antibodies against CRFK cell or FRC lysates initially. All cats administered CRFK cell lysate had detectable antibodies against CRFK cell or FRC lysates on multiple occasions. Of 6 cats vaccinated parenterally, 5 had detectable antibodies against CRFK cell lysate at least once, but all 6 had detectable antibodies against FRC lysate on multiple occasions. Cats administered an intranasal-intraocular vaccine did not develop detectable antibodies against either lysate. Important clinicopathologic or histologic abnormalities were not detected during the study. CONCLUSIONS AND CLINICAL RELEVANCE: Parenteral administration of vaccines containing viruses likely grown on CRFK cells induced antibodies against CRFK cell and FRC lysates in cats. Hypersensitization with CRFK cell proteins did not result in renal disease in cats during the 56-week study.