BACKGROUND: Herpes zoster (HZ) is one of the most common skin diseases caused by viruses. Facial HZ develops when the varicella-zoster virus affects the trigeminal nerve, and alveolar osteonecrosis is a rare complication. However, the exact pathogenesis of postherpetic alveolar osteonecrosis remains unclear. CASE DESCRIPTION: We encountered a patient who presented to the dermatology clinic with facial HZ and tooth exfoliation in the upper right jaw, and panoramic radiography revealed decreased bone density and poor alveolar socket healing in his right maxilla. Biopsy of the alveolar process revealed fragments of nonvital lamellar bone, which were devoid of osteoblasts and osteocytes and were surrounded by numerous neutrophils and bacterial aggregates. Thus, the diagnosis of alveolar osteonecrosis following facial HZ was confirmed. He then underwent resection of the osteonecrotic tissue. The pathological findings of postoperative tissue were similar to those of previous biopsies. Varicella-zoster virus and multiple types of bacteria were detected through next-generation sequencing, and the species of bacteria were consistent with the results of bacterial culture. Antibiotics and valaciclovir were administered during the perioperative period. The patient showed good recovery at the 9-month follow-up. CONCLUSIONS: The coexistence of bacterial and viral infection may play an important role in the pathogenesis of alveolar osteonecrosis following HZ. To our knowledge, we are the first to directly explore microbial pathogens in a case of postherpetic alveolar osteonecrosis through next-generation sequencing and bacterial culture. We recommend that oral examinations be carefully conducted for patients who are diagnosed with facial HZ, even if their facial rashes have faded away. We suggest that a prolonged and full-dose antiviral therapy course may be beneficial for the treatment of facial HZ with intraoral lesions. The implementation of dental preventive measures should be considered for patients with facial HZ. The application of antibiotics and excision of necrotic bone may reduce the abundance of bacteria in lesions and improve wound healing.
Herpes Zoster , Osteonecrosis , Male , Humans , Herpesvirus 3, Human , Herpes Zoster/complications , Herpes Zoster/drug therapy , Tooth Exfoliation/etiology , Osteonecrosis/complications , Anti-Bacterial Agents/therapeutic use
The Bessel beam has become significant in optical research due to its properties such as a long focal depth, self-healing, and non-diffraction. However, conventional methods for generating Bessel beams have drawbacks such as limited flexibility and tunability and the use of bulky optics. These factors lead to the complexity of the optical systems. This paper presents what we believe is a novel approach to generating Bessel beams by utilizing a fully phase-modulated all-dielectric metasurface. The proposed method enables the arbitrary and independent manipulation of cross-polarized and co-polarized components, allowing the creation of Bessel beams featuring multiple polarization conversions when subjected to left-handed circularly polarized (LCP) incidence. To demonstrate the versatility and effectiveness of the method, three metasurfaces with distinct characteristics are designed. The simulated generated Bessel beams exhibit qualities including long focal depth, non-diffraction behavior, self-healing capabilities, and polarization conversion, which align with the theoretical predictions. This work presents novel possibilities for effectively generating and multi-functional application of Bessel beams.
Orbit-induced localized spin angular momentum (OILS) has recently garnered significant attention. This paper introduces periodic edge dislocation (PED) into the tight focusing system. The study delves into the tight focusing characteristics of the radially polarized vortex plane beam with PED, demonstrating that PED serves as a straightforward and effective means of manipulating OILS, especially when both the orbital angular momentum and the polarization of the incident beam are fixed. Our findings indicate that the longitudinal OILS reaches its maximum when the difference between the period of PED and the vortex topological charge is equal to 1. Conversely, when the difference is 0, the transverse OILS reaches its maximum, while the longitudinal OILS reaches its minimum. Similar patterns are also observed in linearly polarized vortex beams. This research proposes a simple and practical way to control OILS, contributing to our understanding of optical orbit-spin coupling.
Introduction: Atopic dermatitis (AD) is one of the most common inflammatory skin diseases. Skin microecological imbalance is an important factor in the pathogenesis of AD, but the underlying mechanism of its interaction with humans remains unclear. Methods: 16S rRNA gene sequencing was conducted to reveal the skin microbiota dynamics. Changes in skin metabolites were tracked by LC-MS metabolomics. We then explored the potential mechanism of interaction by analyzing the correlation between skin bacterial communities and metabolites in corresponding skin-associated samples. Results: Samples from 18 AD patients and 18 healthy volunteers (HVs) were subjected to 16S rRNA gene sequencing and LC-MS metabolomics. AD patients had dysbiosis of the skin bacterial community with decreased species richness and evenness. The relative abundance of the genus Staphylococcus increased significantly in AD, while the abundances of the genera Propionibacterium and Brevundimonas decreased significantly. The relative abundance of the genera Staphylococcus in healthy females was significantly higher than those in healthy males, while it showed no difference in AD patients with or without lesions. The effects of AD status, sex and the presence or absence of rashes on the number of differentially abundant metabolites per capita were successively reduced. Multiple metabolites involved in purine metabolism and phenylalanine metabolism pathways (such as xanthosine/xanthine and L-phenylalanine/trans-cinnamate) were increased in AD patients. These trends were much more obvious between female AD patients and female HVs. Spearman correlation analysis revealed that the genus Staphylococcus was positively correlated with various compounds involved in phenylalanine metabolism and purine metabolic pathways. The genera Brevundimonas and Lactobacillus were negatively correlated with various compounds involved in purine metabolism, phenylalanine metabolism and sphingolipid signaling pathways. Discussion: We suggest that purine metabolism and phenylalanine metabolism pathway disorders may play a certain role in the pathogenic mechanism of Staphylococcus aureus in AD. We also found that females are more likely to be colonized by the genus Staphylococcus than males. Differentially abundant metabolites involved in purine metabolism and phenylalanine metabolism pathways were more obvious in female. However, we should notice that the metabolites we detected do not necessarily derived from microbes, they may also origin from the host.
In this paper, one kind of multi-focusing electric and magnetic field which is sourced from an azimuthally polarized vortex circular hyperbolic umbilic beam (APVCHUB) is presented. After passing through a high NA objective, both the electric and magnetic fields of the APVCHUBs will focus multiple times, and a high-purity longitudinal magnetic field (p q =80%) will be generated. Besides, the mutual induction of the vortex phase and azimuthal polarization changes the electric and magnetic fields' vibration state and intensity distribution, making the longitudinal magnetic field carry an m-order concentric vortex. Our findings suggest that the APVCHUB could have potential applications in magnetic particle manipulation, extremely weak magnetic detection, data storage, semiconductor quantum dot excitation, etc.
In this work, we propose and demonstrate the ring-arrayed Pearcey Gaussian chirp beams (RAPGCBs) synthesized by multiple two-dimensional Pearcey beams. The general analytical formula for the propagation of RAPGCBs is presented. We find that, depending on synthesized number n, the profiles of the beams present different polygonal shapes, and the autofocusing properties can be controlled by chirp factor ß. Furthermore, we study the properties of the RAPGCBs carrying optical vortices (OVs). It shows that a single OV or two positive OVs form an autofocusing hollow field, and opposite OVs will annihilate, which results in greatly increased autofocusing ability. Our experimental results agree with the simulations. Such beams have potential applications in particle trapping and biology medical fields.
We propose and demonstrate a type of multi-focus autofocusing beams, circular hyperbolic umbilic beams (CHUBs), based on the double-active variable caustics in catastrophe theory. The mathematical form is more general compared to circular Airy, Pearcey and swallowtail beams. The CHUBs can generate multi-focus at its optical axis, while the on-axis intensity fluctuates up to two orders of magnitude that of the maximum intensity in the initial plane. Using the concept of topographic prominence, we quantify the autofocusing ability. We construct the criteria for selecting the effective foci, and then explore the influence of related parameters. Our findings suggest that the CHUBs could be a suitable tool for multi-particle manipulation, optical tweezers, optical lattices and related applications.
BACKGROUND: Both programmed cell death-1 (PD-1) inhibitors and lenvatinib, which have a synergistic effect, are promising drugs for tumor treatment. It is generally believed that combination therapy with a PD-1 inhibitor and lenvatinib is safe and effective. However, we report a case of toxic epidermal necrolysis (TEN), a grade 4 toxicity, after this combination therapy. CASE SUMMARY: A 39-year-old male presented with erythema, blisters and erosions on the face, neck, trunk and limbs 1 wk after receiving combination therapy with lenvatinib and toripalimab, a PD-1 inhibitor. The skin injury covered more than 70% of the body surface area. He was previously diagnosed with liver cancer with cervical vertebra metastasis. Histologically, prominent necrotic keratinocytes, hyperkeratosis, liquefaction of basal cells and acantholytic bullae were observed in the epidermis. Blood vessels in the dermis were infiltrated by lymphocytes and eosinophils. Direct immunofluorescence staining was negative. Thus, the diagnosis was confirmed to be TEN (associated with combination therapy with toripalimab and lenvatinib). Full-dose and long-term corticosteroids, high-dose intravenous immunoglobulin and targeted antibiotic drugs were administered. The rashes gradually faded; however, as expected, the tumor progressed. Therefore, sorafenib and regorafenib were given in succession, and the patient was still alive at the 10-mo follow-up. CONCLUSION: Cautious attention should be given to rashes that develop after combination therapy with PD-1 inhibitors and lenvatinib. Large-dose and long-course glucocorticoids may be crucial for the treatment of TEN associated with this combination treatment.
Melanoma is an aggressive malignant skin tumour with an increasing global incidence. However, current treatments have limitations owing to the acquired tumour drug resistance. Ferroptosis is a recently discovered form of programmed cell death characterised by iron accumulation and lipid peroxidation and plays a critical role in tumour growth inhibition. Recently, ferroptosis inducers have been regarded as a promising therapeutic strategy to overcome apoptosis resistance in tumour cells. In this study, we reported that nobiletin, a natural product isolated from citrus peel, and exhibited antitumour activity by inducing ferroptosis in melanoma cells. Subsequently, we further explored the potential mechanism of nobiletin-induced ferroptosis, and found that the expression level of glycogen synthase kinase 3ß (GSK3ß) in the skin tissue of patients with melanoma was significantly reduced compared to that in the skin of normal tissue. Additionally, nobiletin increased GSK3ß expression in melanoma cells. Moreover, the level of Kelch-like Ech-associated protein-1 (Keap1) was increased, while the level of nuclear factor erythroid 2-related factor 2 (Nrf2), and haem oxygenase-1 (HO-1) was decreased in nobiletin-treated melanoma cells, suggesting that the antioxidant defence system was downregulated. Furthermore, knockdown of GSK3ß significantly reduced nobiletin-induced ferroptosis and upregulated the Keap1/Nrf2/HO-1 signalling pathway, while the opposite was observed in cells overexpressing GSK3ß. In addition, molecular docking assay results indicated that nobiletin showed strong binding affinities for GSK3ß, Keap1, Nrf2, and HO-1. Taken together, our results demonstrated that nobiletin could induce ferroptosis by regulating the GSK3ß-mediated Keap1/Nrf2/HO-1 signalling pathway in human melanoma cells. Hence, nobiletin stands as a promising drug candidate for melanoma treatment with development prospects.
ABSTRACT: Eccrine porocarcinoma (EPC) is a rare malignant sweat gland tumor that accounts for approximately 0.005% of all cutaneous carcinomas. It favors the lower extremities. Only 3% of EPCs are on the hand, and only 6 cases occurring specifically on fingers have been previously documented. However, we met a patient with EPC presenting the primary lesion on the left thumb and an extensive cutaneous metastasis on the left forearm. Pathologic findings of axillary lymph nodes confirmed lymphatic metastasis.
Eccrine Porocarcinoma/pathology , Sweat Gland Neoplasms/pathology , Thumb/pathology , Humans , Male , Middle Aged , Neoplasm Metastasis/pathology
Hemophagocytic lymphohistiocytosis (HLH) is a high-fatality disease caused by hereditary or acquired immune dysfunction, and is characterized by pathological inflammatory response. Primary HLH (pHLH) has hereditary genetic defects, and secondary HLH (sHLH) is caused by a variety of underlying diseases. Here, we report the case of a patient with aggressive natural killer cell leukemia and HLH-related gene defects who achieved long-term survival after treatment. A 20-year-old man presented to our hospital with symptoms of fever and fatigue. Investigations revealed splenomegaly, cytopenia, hyperferritinemia, hypofibrinogenemia, elevated levels of soluble CD25 (sCD25), and hemophagocytosis in bone marrow. Bone marrow flow cytometry showed 23.4% abnormal natural killer cells, the cells were CD2, CD7, CD16, CD94, NKG2A positive, met the diagnosis of aggressive NK-Cell leukemia. Investigation of the patient's pedigree revealed that mutations of pHLH-related genes (LYST and UNC13D) were inherited from his father and mother, but neither of the parents had the disease. The patient received hematopoietic stem-cell transplantation (HSCT), after which he achieved complete remission. As of 2020-10-19, the patient's survival has exceeded 3 years, and he has returned to his normal life. A variety of factors contribute to the onset of HLH, and this case gives greater insight into the etiology of HLH. Allogeneic HSCT is a key treatment for HLH patients with underlying genetic mutations.
Hematopoietic Stem Cell Transplantation , Lymphohistiocytosis, Hemophagocytic , Adult , Bone Marrow , Fever , Humans , Lymphohistiocytosis, Hemophagocytic/genetics , Lymphohistiocytosis, Hemophagocytic/therapy , Male , Membrane Proteins , Remission Induction , Young Adult
We theoretically and experimentally study the propagation characteristics of elliptical Airy vortex beams (EAVBs) with a circular concentric vortex. It is found that EAVBs inherit the abruptly autofocusing properties of the circular Airy beams (CABs), but EAVBs will have a better autofocusing performance than circular Airy vortex beams (CAVBs) under certain conditions. It is also found that the initial m-order concentric vortex of EAVBs splits into |m| first-order vortices at the autofocusing plane, and the focusing pattern splits into |m|+1 bright spots with the pattern's tilting direction related to the sign of m [m is the topological charge (TC) of the vortex]. These characteristics of EAVBs may have potential applications in TC detection, optical micromanipulation, communications, and other fields.
A novel noise-suppressing and lock-free interferometer is proposed and experimentally demonstrated in the study of the quantum non-destructive (QND) interaction of cold atoms. A QND measurement based on far-off resonant dispersive probing is usually carried out by a Mach-Zehnder type interferometer. It is an experimental challenge in its own right to reduce the classical noise, such as acoustic noise, phase noise and amplitude noise of lasers, and to lock the interferometer at the white-light position that corresponds to a nearly zero path-length difference. Here, we report an interferometer with an inserted acousto-optic modulator (AOM). It is noise immune and lock-free in principle. The experiments show that the new interferometer is able to measure cold atoms for more than 30 minutes and reduce the phase noise by about 30 dB.
We propose and demonstrate a new, to the best of our knowledge, kind of partially coherent vector beam called the partially coherent radially polarized circular Airy beam (PCRPCAB). The PCRPCAB inherits the autofocusing ability of the radially polarized circular Airy beam (RPCAB) and can create an optical potential well at the center of the beam, whose depth can be adjusted by changing the coherent width. We find that, as coherent width decreases, the intensity becomes higher in the dark notch caused by the polarization singularity, and the singularity of the degree of polarization (DOP) remains along propagation, with its waist controllable by the coherent width. Our results make the PCRPCAB a good candidate for optical micromanipulation, disordered optical lattices, etc.
We proposed and realized a low noise measurement method based on differential optical interferometer to measure trapped cold atoms in a magneto-optical trap (MOT). The configuration is based on a Mach-Zehnder type interferometer, which is composed of two beams of different frequencies. A long-term stability in phase monitor has been obtained by use of the vibration immune mechanism through subtraction of the interferograms imaged on the two photodetectors. With this new configuration, the noise caused by environmental perturbation is greatly reduced at low frequency while the signal of phase shift keeps a good long-term stability.
In this work, the radiation force on a Rayleigh dielectric particle induced by the partially coherent circular Airy beam (PCCAB) is investigated. Our numerical results show that the PCCAB can be used to trap and manipulate particles. The radiation force distribution and trapping stability have been analyzed under different coherent lengths. It is found that, with the increase of the spatial coherent length, the radiation force is increased and the particle can be stably trapped at more points. Therefore, the radiation force as well as the depth of potential well can be effectively modulated by controlling the spatial coherent length in optical micromanipulation. The trapping properties of PCCAB have also been studied under other different parameters, including the scale factor and initial radius.
BACKGROUND: Hedgehog (Hh) signaling is involved in the pathogenesis of tumors. By performing gene chip analysis, we predicted that Hh signaling might regulate multiple downstream pathways in acute myeloid leukemia (AML). METHODS: In this study, the potential role of the Hh pathway in refractory AML, and the impact of Hh expression on clinical prognosis were examined. We also investigated the role of the Hh inhibitor NVP-LDE225 in reversing drug resistance of refractory primary AML cells in vitro and the roles of multiple drug-resistant HL60/Adriamycin-resistant cells in vitro and in vivo (in a xenograft mouse model). Finally, we explored the underlying mechanisms. RESULTS: Hh pathway was highly active in chemotherapy-resistant AML cells; by contrast, activation was less pronounced in chemosensitive cells and non-refractory primary cells. Strong activation of this pathway was associated with higher recurrence rates and poorer relapse-free and overall survival. NVP-LDE225 inhibited MRP1 protein expression, increased intracellular accumulation of Adriamycin, and reversed chemotherapeutic resistance. These effects were likely mediated through inhibition of the IGF-1R/Akt/MRP1 pathway. In the AML xenograft mouse model, NVP-LDE225 plus Adriamycin resulted in marked tumor regression. CONCLUSION: These findings suggest that targeting the Hh pathway might be a therapeutic avenue for overcoming MDR resistance and preventing refractory AML.
We propose a new kind of light beam with both autofocusing ability and radial-variant distribution of polarization states, named radial-variant vector circular Airy beam (RVCAB). The polarization transitions in the focus of RVCABs are simulated based on vectorial angular spectrum formalism and theoretically explained by Stokes parameters. Our results reveal the dependence of the pure polarization state near the focus on the distribution of polarization states in the initial plane, which may lead to manipulation of the polarization state near the focus via controlling the distribution of polarization states in the initial plane.
We found a new type of noncircular symmetrical Airy beam called an elliptical Airy beam (EAB). Using a simple single-pixel checkerboard hologram method, we achieved the EAB in an experiment. We observed its unique property of double focusing and the ability of the energy to flow towards the endpoints of the long axis during propagation. These particular properties will have some potential applications.
We propose a multifocus autofocusing Airy beam (MAAB) by modifying the frequency spectrum of a symmetric Airy beam (SAB) with a Gaussian band elimination filter. Unlike the original SAB, there are four off-axis foci at the autofocusing plane of the MAAB. The MAAB has a better abruptly autofocusing property than the original SAB. In addition, with the increase of the normalized intensity at the first peak, the focal position (second peak) of the MAAB remains almost the same, whereas the position of the first peak shifts along the propagation direction gradually. These unique characteristics of MAAB have been demonstrated by experiment and indicate potential applications in various fields.
