Mycorrhizal colonization had little effect on growth of Carex thunbergii but inhibited its nitrogen uptake under deficit water supply.

BACKGROUND AND AIMS: Plant nitrogen (N) acquisition via arbuscular mycorrhizal fungi (AMF) serves as a dominant pathway in the N nutrition of many plants, but the functional impact of AMF in acquisition of N by wetland plants has not been well quantified. Subtropical lake-wetland ecosystems are characterized by seasonal changes in the water table and low N availability in soil. Yet, it is unclear whether and how AMF alters the N acquisition pattern of plants for various forms of N and how this process is influenced by soil water conditions. METHODS: We performed a pot study with Carex thunbergii that were either colonized by AMF or not colonized and also subjected to different water conditions. We used 15N labelling to track plant N uptake. KEY RESULTS: Colonization by AMF had little effect on the biomass components of C. thunbergii but did significantly affect the plant functional traits and N acquisition in ways that were dependent on the soil water conditions. The N uptake rate of AMF-colonized plants was significantly lower than that of the non-colonized plants in conditions of low soil water. A decreased NO3- uptake rate in AMF-colonized plants reduced the N:P ratio of the plants. Although C. thunbergii predominantly took up N in the form of NO3-, higher water availability increased the proportion of N taken up as NH4+, irrespective of the inoculation status. CONCLUSIONS: These results emphasize the importance of AMF colonization in controlling the N uptake strategies of plants and can improve predictions of N budget under the changing water table conditions in this subtropical wetland ecosystem.

Density-dependent plant-soil feedbacks of two plant species affected by plant competition.

Both plant-soil feedbacks (PSF) and plant competition drive plant community assembly, but their interactive effects have rarely been investigated, and the role of community composition in modulating these interactions is unknown. We conducted a fully reciprocal experiment with two plant species (Polygonum criopolitanum and Carex thunbergii) and their associated soil biota communities to untangle the relative importance of PSF and competition. The two species were grown either in monoculture or in mixed plantings, with various densities of each plant's neighbor, and either in the presence or absence of the soil biota associated with either species. When grown individually, each plant species showed a neutral PSF in the presence of its own soil biota, while feedbacks in competition were predominantly negative. P. criopolitanum produced more biomass in the presence of soil biota than in autoclaved soil, but the opposite was observed for C. thunbergii. In competition, both plant species were suppressed by its presence of their own soil biota, and neither had a competitive advantage, resulting in a significant negative pairwise PSF in most cases. C. thunbergii also showed stronger per capita effects than did P. criopolitanum. The predication on species coexistence largely depended on the presence of soil biota, the soil origin, and the plant species ratio. When planted in equal proportion, P. criopolitanum and C. thunbergii only coexisted in the presence of soil biota of P. criopolitanum, while C. thunbergii usually excluded P. criopolitanum under all other conditions. When the density of the neighboring plant increased, either species was excluded in the presence of their own soil biota. However, in the absence of a soil biota, there was a priority effect, giving an advantage to the species that was planted first. Our study revealed that the strength and direction of these interactions were altered by interspecific competition at the community level, and both the identity and the density of the competitors must be taken into account in interpreting plant community assembly processes.

Nitrogen Uptake by Two Plants in Response to Plant Competition as Regulated by Neighbor Density.

Plant species may acquire different forms of nitrogen (N) to reduce competition for the same resource, but how plants respond to neighbors with different densities in their N uptake is still poorly understood. We investigated the effects of competition regime on the uptake of different N forms by two hygrophytes, Carex thunbergii and Polygonum criopolitanum, by conducting a hydroponic test of excised roots and an in situ experiment in a subtropical wetland ecosystem. The two species were grown either in monocultures or mixtures with various neighbor densities. Root functional traits and N uptake rates of different N forms were measured. Our results showed that N uptake was mainly determined by N form, rather than species identity. Both species were able to use organic N sources, but they took up relatively more N supplied as NO 3 - than as NH 4 + or glycine, irrespective of competition treatments. Both species preferred NO 3 - when grown in monoculture, but in the presence of competitors, the preference of fast-growing C. thunbergii persisted while P. criopolitanum acquired more NH 4 + and glycine, with stronger responses being observed at the highest neighbor density. The hydroponic test suggested that these divergences in N acquisition between two species might be partially explained by different root functional traits. To be specific, N uptake rates were significantly positively correlated with root N concentration and specific root length, but negatively correlated with root dry matter content. Our results implicated that C. thunbergii has a competitive advantage with relatively more stable N acquisition strategy despite a lower N recovery than P. criopolitanum, whereas P. criopolitanum could avoid competition with C. thunbergii via a better access to organic N sources, partly mediated by competition regimes.

Site conditions interact with litter quality to affect home-field advantage and rhizosphere effect of litter decomposition in a subtropical wetland ecosystem.

The home-field advantage (HFA) hypothesis predicts that plant litter would decompose more quickly beneath its own plant species in the soil than beneath other plant species. Theoretically, HFA can be induced by the rhizosphere of growing plants, due to so-called rhizosphere effect (RE). Despite growing evidence for the site condition-dependence of both effects, few work has be conducted to explore how site climate, vegetation type and soil properties interact to affect RE and HFA, and especially limited in situ representation from subtropical wetland systems. In a field experiment, we reciprocally incubated three root litter species (Rumex dentatus L., Carex thunbergii Steud., and Polygonum cripolitanum Hance) along a hydroperiod gradient in a subtropical wetland, which differed mainly with respect to vegetation and soil microclimate, with and without growing plants. The occurrence and magnitude of HFA and RE were mainly determined by litter quality and were stage-specific. Collectively, we detected significant HFA with chemically-recalcitrant litter from C. thunbergii and P. cripolitanum, but only at the first stage of decomposition. The presence of growing plants generally reduced litter decomposition, but the magnitude of the response was species-specific, with the positive effects detected only for root litters from C. thunbergii at the first stage of decomposition. In addition, we did not find a significant relationship between HFA and RE, indicating that plant species that produce litters exhibiting HFA may not accelerate litter decomposition via RE at same time. Structural equation models (SEM) revealed that site microclimate factors were conducive with soil properties in regulating C dynamics. Overall, soil microclimate in this wetland ecosystem was likely important in driving C cycling, either directly by changing environmental conditions, litter quality, and plant trait spectra, or indirectly by interrupting the interactions between litter and decomposers.

Litter mixture dominated by leaf litter of the invasive species, Flaveria bidentis, accelerates decomposition and favors nitrogen release.

In natural ecosystems, invasive plant litter is often mixed with that of native species, yet few studies have examined the decomposition dynamics of such mixtures, especially across different degrees of invasion. We conducted a 1-year litterbag experiment using leaf litters from the invasive species Flaveria bidentis (L.) and the dominant co-occurring native species, Setaria viridis (L.). Litters were allowed to decompose either separately or together at different ratios in a mothproof screen house. The mass loss of all litter mixtures was non-additive, and the direction and strength of effects varied with species ratio and decomposition stage. During the initial stages of decomposition, all mixtures had a neutral effect on the mass loss; however, at later stages of decomposition, mixtures containing more invasive litter had synergistic effects on mass loss. Importantly, an increase in F. bidentis litter with a lower C:N ratio in mixtures led to greater net release of N over time. These results highlight the importance of trait dissimilarity in determining the decomposition rates of litter mixtures and suggest that F. bidentis could further synchronize N release from litter as an invasion proceeds, potentially creating a positive feedback linked through invasion as the invader outcompetes the natives for nutrients. Our findings also demonstrate the importance of species composition as well as the identity of dominant species when considering how changes in plant community structure influence plant invasion.

[Effects of tillage methods on soil physicochemical properties and biological characteristics in farmland: A review].

Tillage methods affect soil heat, water, nutrients and soil biology in different ways. Reasonable soil management system can not only improve physical and chemical properties of the soil, but also change the ecological process of farmland soil. Conservation tillage can improve the quality of the soil to different degrees. For example, no-tillage system can effectively improve soil enzyme activity. No tillage and subsoiling tillage can provide abundant resources for soil microbe' s growth and reproduction. No tillage, minimum tillage and other conservation tillage methods exert little disturbance to soil animals, and in turn affect the quantity and diversity of the soil animals as well as their population structure. Effects of different tillage methods on soil physical and chemical properties as well as biological characteristics were reviewed in this article, with the soil physical and chemical indices, enzyme activities, soil microbe diversity and soil animals under different tillage patterns analyzed. The possibility of soil quality restoration with appropriate tillage methods and the future research direction were pointed out.

Potential gene flow of two herbicide-tolerant transgenes from oilseed rape to wild B. juncea var. gracilis.

Four successive reciprocal backcrosses between F(1) (obtained from wild Brassica juncea as maternal plants and transgenic glyphosate- or glufosinate-tolerant oilseed rape, B. napus, as paternal plants) or subsequent herbicide-tolerant backcross progenies and wild B. juncea were achieved by hand pollination to assess potential transgene flow. The third and forth reciprocal backcrosses produced a number of seeds per silique similar to that of self-pollinated wild B. juncea, except in plants with glufosinate-tolerant backcross progeny used as maternal plants and wild B. juncea as paternal plants, which produced fewer seeds per silique than did self-pollinated wild B. juncea. Germination percentages of reciprocal backcross progenies were high and equivalent to those of wild B. juncea. The herbicide-tolerant first reciprocal backcross progenies produced fewer siliques per plant than did wild B. juncea, but the herbicide-tolerant second or third reciprocal backcross progenies did not differ from the wild B. juncea in siliques per plant. The herbicide-tolerant second and third reciprocal backcross progenies produced an amount of seeds per silique similar to that of wild B. juncea except for with the glufosinate-tolerant first and second backcross progeny used as maternal plants and wild B. juncea as paternal plants. In the presence of herbicide selection pressure, inheritance of the glyphosate-tolerant transgene was stable across the second and third backcross generation, whereas the glufosinate-tolerant transgene was maintained, despite a lack of stabilized introgression. The occurrence of fertile, transgenic weed-like plants after only three crosses (F(1), first backcross, second backcross) suggests a potential rapid spread of transgenes from oilseed rape into its wild relative wild B. juncea. Transgene flow from glyphosate-tolerant oilseed rape might be easier than that from glufosinate-tolerant oilseed rape to wild B. juncea. The original insertion site of the transgene could affect introgression.

Response of wild Brassica juncea populations to glyphosate.

BACKGROUND: Wild Brassica juncea (L.) is a troublesome arable land weed and ruderal. It is critical to understand the responses of this weed to herbicides, because the assessment of its susceptibility profile has important ecological and evolutionary consequences for future cultivation of herbicide-tolerant oilseed rape. The response of 31 wild populations from different geographic origins in China to glyphosate was evaluated with two bioassay methods, and variable responses were found in initial studies. Dose-response assays were conducted to characterize the extreme populations further, and shikimate accumulation in vivo was determined using a spectrophotometric method. RESULTS: On the basis of ID(50) values, the resistance ratios (R/S) were 5.85 and 4.19 for two glyphosate-resistant B. juncea populations in germination tests, whereas they were 4 times more resistant to glyphosate in spray tests. There were differences in shikimate accumulation patterns between the two biotypes. Shikimate concentrations in resistant populations began to decline from 6 days after treatment (DAT), while they increased continually in susceptible populations. CONCLUSION: The results obtained suggest that the populations responded differentially to glyphosate, and this variability may provide the genetic basis for evolution of individuals with increased resistance to glyphosate, with important implications for herbicide resistance management, especially in the context of risk assessment of glyphosate-tolerant crops.