Training Volume and High-Speed Loads Vary Within Microcycle in Elite North American Soccer Players.

ABSTRACT: Ishida, A, Draper, G, Wright, M, Emerson, J, and Stone, MH. Training volume and high-speed loads vary within microcycle in elite North American soccer players. J Strength Cond Res 37(11): 2229-2234, 2023-The purposes of this study were to reduce dimensionality of external training load variables and examine how the selected variables varied within microcycle in elite North American soccer players. Data were collected from 18 players during 2018-2020 in-seasons. Microcycle was categorized as 1, 2, 3, 4, 5 days before match day (MD-1, MD-2, MD-3, MD-4, and MD-5, respectively). Training load variables included total distance, average speed, maximum velocity, high-speed running distance (HSR), average HSR, HSR efforts, average HSR efforts, sprint distance, average sprint distance, sprint efforts, average sprint efforts, total PlayerLoad, and average PlayerLoad. The first principal component (PC) can explain 66.0% of the variances and be represented by "high-speed load" (e.g., HSR and sprint-related variables) with the second PC relating to "volume" (e.g., total distance and PlayerLoad) accounting for 17.9% of the variance. Average sprint distance and total distance were selected for further analysis. Average sprint distance was significantly higher at MD-3 than at MD-2 (p = 0.01, mean difference = 0.36 m•minute-1, 95% confidence intervals [CIs] = 0.07-0.65 m•minute-1) and MD-4 (p = 0.012, mean difference = 0.26 m•minute-1, 95% CIs = 0.10-0.41 m•minute-1). Total distance was significantly higher at MD-3 than at MD-1 (p < 0.001, mean difference = 1,465 m, 95% CIs = 1,003-1926 m), and MD-2 (p < 0.001, mean difference = 941 m, 95% CIs = 523-1,360 m). Principal component analysis may simplify reporting process of external training loads. Practitioners may need to choose "volume" and "high-speed load" variables. Elite North American Soccer players may accumulate higher average sprint distance at MD-3 than at other training days.

The difference between several neuromuscular tests for monitoring resistance-training induced fatigue.

The purposes of this study were to investigate the acute effects of resistance training protocol on kinetic changes in squat jump (SJ), shortened isometric mid-thigh pull (IMTP), and isometric squat (ISQ) and to examine the relationship of dynamic maximum strength with performance changes over 48 hours in resistance-trained individuals. Participants completed performance tests at pre-, post-24 hours, and post-48 hours resistance training protocol (Baseline, Post24, and Post48). The training protocol consisted of 5 sets of 10 repetitions of back squat (BSQ) at 60% of 1 repetition maximum (1RM). SJ variables included jump height (JH), peak power (PP), and relative PP. For the IMTP and ISQ, isometric peak force (IPF), relative IPF, rate of force development at 250 milliseconds (RFD250), and impulse at 250 milliseconds (IMP250) were calculated. Significant decreases were observed from Baseline to Post24 (p = 0.023, Cohen's dz effect size [dz] = 1.00) and Post48 (p = 0.032, dz = 0.94) in SJ JH. IMTP IMP250 significantly decreased from Baseline to Post48 (p = 0.046, dz = 0.88). Significant negative correlation was found between relative 1RM BSQ and the changes from Baseline to Post48 in ISQ RFD250 (p = 0.046,r = -0.61). Acute performance decreases might remain until 48 hours after resistance training in explosive strength and impulse regardless of isometric testing type.

Physiological and biomechanical responses to exercise on two different types of rowing ergometers in NCAA Division I oarswomen.

BACKGROUND: Stationary (SE) and dynamic (DE) rowing ergometers, that are utilized for indoor training and physical assessment of competitive rowers, may elicit different physiological and biomechanical responses. The present study used SE and DE ergometers to examine submaximal and peak physiological and biomechanical responses during an incremental rowing test. METHODS: Twelve National Collegiate Athletic Association (NCAA) Division I oarswomen performed seven-stage rowing tests with the last stage performed with maximal effort. Heart rate (HR), lactate (LA), oxygen uptake (VO2), ventilation (VE), stroke rate (SR), gross efficiency (GE), and rating of perceived exertion (RPE) were obtained; while trunk, hip, knee, shoulder, and elbow ranges of motion (ROM) were measured. RESULTS: SR was higher at maximal stage DE (29.3 vs. 34.8 strokes/min, p = 0.018, d = 1.213). No difference occurred in responses of maximal stage HR, RPE, VO2, VE, LA, or GE between the two ergometers. Submaximal LA and SR were greater on the DE for all submaximal stages. Submaximal VE was greater on the DE for all submaximal stages except Stage 3 (p = 0.160, d = 0.655). VO2 was higher on the DE Stages 2-5. GE was higher on the SE for Stages 2-5. Athletes showed increased trunk (p = 0.025, [Formula: see text] = 0.488) and knee (p = 0.004, [Formula: see text] = 0.668) ROM on SE. CONCLUSION: Rowing on the DE appears to elicit a greater stroke rate and more optimal joint angles especially at high intensities. Hence, the DE is worthy of consideration as a preferred ergometer for women rowers.

Does Prematch Neuromuscular Performance Affect Running Performance in Collegiate Elite Female Soccer?

ABSTRACT: Ishida, A, Draper, G, White, JB, and Travis, SK. Does prematch neuromuscular performance affect running performance in collegiate elite female soccer? J Strength Cond Res 37(4): 854-858, 2023-The purpose of this study was to investigate whether prematch neuromuscular performance affected match physical performance by player position in Division I collegiate female soccer. Fourteen players participated (20.7 ± 1.3 years; 165.1 ± 6.0 cm; 63.3 ± 7.0 kg) in this study. Players were selected based on being free from injuries, playing for a duration of ≥72 minutes, and match intervals were ≥2 days. Data included 73 observations ( n = 5 defenders, n = 7 midfielders, and n = 2 forward) from 14 official matches. Prematch neuromuscular performance was assessed using countermovement jump (CMJ) with polyvinyl chloride pipe on dual force plates. Countermovement jump variables included jump height (JH) and relative peak power (RPP). Match physical performance included average speed, high-speed running (HSR), and number of high accelerations and decelerations. Linear mixed model was compiled with fixed effects of loaded with fixed effect of JH, RPP, and position and random effects per individual player. In CMJ and player position model, RPP was a significant predictor for average HSR ( p = 0.003) while fixed effects of JH and position were not significant ( p = 0.15-0.86). The fixed and random effects explained 34.8 and 42.1% of variances in average HSR. In CMJ-only model, RPP significantly predicted the variances in average HSR ( p = 0.001). The fixed effects and random effects of the model explained 22.7 and 51.8% of the variance in average HSR. However, position-only was not a significant predictor for average HSR in the position-only model ( p = 0.26 and 0.33). Prematch CMJ RPP may be the greatest predictor of average HSR in collegiate female soccer.

Do environmental temperatures and altitudes affect physical outputs of elite football athletes in match conditions? A systematic review of the 'real world' studies.

Players involved in the various football codes compete throughout the calendar year around the world. Therefore, environmental stressors such as temperature and altitude should be considered in preparation for, and during, matches. We aimed to systematically review the observational and quasi-experimental studies that have been specifically designed to quantify the effects of temperature (hot or cold) high altitude on in-match physical performance indicators. A search of electronic databases (Web of Science, Scopus, SPORTDiscus, PubMed/MEDLINE) was conducted, with 19,424 papers identified as relevant. Following sifting in relation to the eligibility criteria, 12 papers were deemed directly relevant. The reviewed studies scored 6-9 (on a 0-9 scale) for quality assessment using a previously used scale. The major outcome variables relevant to the current review were total distance (m), high-speed running (m) and high-speed runs (count) measured during matches. Standardized effect sizes (ES) were heterogeneous across studies for total distance (ES: -0.96 to -0.14) and high-speed running (ES: -0.69 to 0.12) for >1000 m vs sea-level, time spent at the given altitude being a putative factor for this heterogeneity. Heat had mainly detrimental effects on performance, but ES were, again, heterogeneous across studies (ES: -1.25 to 0.26), dependent on temperature. Given the small number of studies that involved mostly male athletes, and large heterogeneity across studies, more research needs be conducted on physical performance in these environmental conditions, with attention paid to standardizing outcomes and broadening the approaches of studies to guide future decision-making in professional sporting environments.

Intrasession and Intersession Reliability of Isometric Squat, Midthigh Pull, and Squat Jump in Resistance-Trained Individuals.

ABSTRACT: Ishida, A, Suarez, DG, Travis, SK, Slaton, JA, White, JB, Bazyler, CD, and Stone, MH. Intrasession and intersession reliability of isometric squat, midthigh pull, and squat jump in resistance-trained individuals. J Strength Cond Res 37(1): 18-26, 2023-The purposes of this study were to investigate intrasession and intersession reliability of variables obtained from squat jump (SJ), shortened isometric midthigh pull (IMTP), and isometric squat (ISQ) protocols and to evaluate relationships between isometric and dynamic performance and 1 repetition maximum (1RM) back squat (BSQ). Eleven moderately resistance-trained men participated (27.8 ± 3.9 years; 175.0 ± 7.2 cm; 87.2 ± 11.4 kg). Subjects completed familiarization in the IMTP and ISQ, followed by 1RM BSQ at least 48 hours before the first performance test. Two performance tests occurred at 7-day intervals including SJ, IMTP, and ISQ. SJ variables included jump height (SJH), body mass (BM), peak force (PF), and peak power (PP). Isometric midthigh pull and ISQ variables included isometric peak force (IPF); relative IPF; rate of force development at 90, 200, and 250 milliseconds; and impulse at 90, 200, and 250 milliseconds. SJ, IMTP, and ISQ kinetic variables were considered reliable if intraclass correlations (ICCs) and coefficients of variations (CVs) were >0.80 and <10%. Intrasession and intersession reliability criteria were met for SJH, BM, PF, and PP (ICC = 0.91-1.00, CV = 0.5-9.1%). Isometric peak force and impulse at 200 and 250 milliseconds met intrasession and intersession reliability criteria for IMTP and ISQ (ICC = 0.90-0.99, CV = 2.1-8.1%). Significant large correlation was observed between 1RM BSQ and ISQ peak force (p = 0.038, r = 0.63), but not between 1RM BSQ and shortened IMTP peak force (p = 0.11, r = 0.50). Shortened IMTP and ISQ peak force and impulse are reliable kinetic variables, and ISQ peak force is indicative of 1RM BSQ in moderately resistance-trained men.

Player Position Affects Relationship Between Internal and External Training Loads During Division I Collegiate Female Soccer Season.

ABSTRACT: Ishida, A, Travis, SK, Draper, G, White, JB, and Stone, MH. Player position affects relationship between internal and external training loads during Division I collegiate female soccer season. J Strength Cond Res 36(2): 513-517, 2022-The purpose of this study was to investigate how competition phase and player position affect the relationship between internal and external training loads (ITL and ETL, respectively) in collegiate female soccer. Seventeen players participated (21.8 ± 1.7 years; 165.1 ± 6.2 cm; and 63.7 ± 7.9 kg). Nineteen match-plays (10 nonconference and 9 conference) were completed during the 2019 competitive season, including 270 observations of 17 players (defenders = 5, midfielders = 9, and forwards = 3). Internal training load was assessed using session rating of perceived exertion (sRPE). External training load included total distance and high-speed running (HSR) distance. A linear mixed model was compiled with fixed effects of total distance, HSR, competition phase, and player position (defenders, midfielders, and forwards) and random effects of player. There were statistically significant main effects for total distance (p < 0.001), HSR (p = 0.047) and player position (p = 0.045) on the prediction model of sRPE. However, the main effect of competition phase did not statistically contribute to the prediction model of sRPE (p = 0.38). In the final model, total distance (p < 0.001) and player position for forwards (p = 0.008) were significant predictors of sRPE. However, there was no statistically significant fixed effect of HSR on sRPE (p = 0.15). The final model explained 60.6% of the variance in sRPE (R2 = 0.60), whereas the random effect also explained 6.1% of the variance (R2 = 0.06). Our findings indicated that total distance and player position were strong predictors of sRPE. The relationship between ITL and ETL should be monitored by player position in female soccer players.

Early Cochlear Implantation Allows Deaf Children to Control Voice Pitch and Loudness independently.

OBJECTIVE: Congenitally deaf children with a cochlear implant (CI) improve their speech production intelligibility, but their prosody, including stress and intonation produced by voice pitch and loudness changes, often differs from normal hearing people, which affects their fluent oral communication. This study is aimed to clarify abnormal characteristics of voice pitch and loudness control in CI recipients using a newly developed visually guided pitch change task. METHODS: We included 13 normal hearing volunteers (NH group) and 17 congenitally deaf CI users who showed good speech intelligibility. The 17 CI users were divided into Early-CI and Late-CI groups, based on their age at implantation of 2.5 years. Using the visually guided pitch change task and acoustic voice analysis of sustained vowels, we assessed their vocal skills to maintain constant pitch and loudness and intentionally change the voice pitch independent from loudness. At the time of examination, the mean age was 24.1, 12.7, and 18.9 years in the NH, Early-CI, and Late-CI groups. RESULTS: During constant vowel production, Early-CI and Late-CI groups showed more significant fluctuations in pitch and loudness than the NH group. However, when focusing on intentionally changing only the pitch of the voice, the Early-CI group was significantly better than the Late-CI group and comparable to the NH group in terms of their ability to change vocal pitch independently from loudness. CONCLUSION: Among congenitally deaf patients, age at implantation was associated with the development of voice skills to control voice pitch and loudness separately, which may influence their fluent prosody production.

Seasonal Changes and Relationships in Training Loads, Neuromuscular Performance, and Recovery and Stress State in Competitive Female Soccer Players.

Background: The purpose of this study was to examine seasonal changes in training load (TL), neuromuscular performance, subjective recovery, and stress state, and to investigate the relationships between acute and chronic TL and neuromuscular performance in competitive female soccer players. Methods: Nine competitive female soccer players (20.0 ± 1.7 years; 60.3 ± 6.3 kg; 164.0 ± 5.8 cm) completed the Short Recovery and Stress Scale and the countermovement jump (CMJ) with polyvinyl chloride pipe (CMJ0) and 20 kg barbell (CMJ20) at 2-3 h before 1st match (NC1), 6th match (NC2), 9th match (C1), and 15th match (C2) of the competitive season. TL included total distance, high-speed running, and PlayerLoad. Acute and chronic TL was calculated by using the average of 2 days (D2), 7 days (D7), and 21 days (D21) prior to four different match play. Results: Significant decreases were found from NC1 to C1 in D7 total distance [p = 0.03, Cohen's effect size (dz) = 1.40]. D7 total distance and PlayerLoad significantly decreased from NC to C1 and C2 (p = 0.001-0.01, dz = 1.40-1.72). Significant increases were observed from NC1 to C1 in CMJ0 jump height (p = 0.03, dz = 1.40), (p = 0.021, dz = 1.44), and peak power (p = 0.03, dz = 1.32). Significant negative correlations were observed for D7 total distance and CMJ0 jump height (p = 0.02, r = 0.79) and peak power (p = 0.03, r = 0.71) at C2, while significant positive correlations were observed at C1 for D7 PlayerLoad and CMJ0 jump height (p = 0.02, r = 0.80). Conclusion: Polyvinyl chloride pipe (CMJ0) jump height and peak power may increase from preseason to the midcompetitive season. Seasonal variations may affect the relationships between D7 TL and CMJ0 performance.

Short-Term Periodized Programming May Improve Strength, Power, Jump Kinetics, and Sprint Efficiency in Soccer.

The purpose of this study was to examine if short-term periodized programming may improve strength, power, jump kinetics, and sprint efficiency in soccer. Seventeen players (19.6 ± 1.6 yrs; 73.8 ± 8.2 kg; 1.77 ± 0.6 m) were divided into two groups based on mean isometric midthigh pull peak force (IPF) (stronger and weaker) and squat jump (SJ) peak power (PP) (higher power and lower power). Eight weaker players were included in the lower power group, while six stronger players were included in the higher power group. Block periodization was adopted to design strength training consisting of 3-week strength endurance and 4-week maximum strength blocks. Performance data included SJ with polyvinyl chloride pipe (SJ0), 20 kgs bar (SJ20), and 40 kgs (SJ40) bar and 20 m sprint across three time points (baseline: TB; post-block 1: T1; post-block 2: T2). Stronger group showed significant increases from TB to T2 in SJ20 peak power (PP), net impulse, and allometrically-scaled PP (p = 0.005 to 0.01, ES = 0.32 to 0.49). Weaker group demonstrated moderate to large increases from TB to T2 in SJ20, allometrically-scaled peak force (PF), PP, and allometrically-scaled PP (p = <0.001 to 0.04, ES = 1.41 to 1.74). Lower power group showed significant increases from TB to T2 in SJ20 allometrically-scaled PF, net impulse, PP, and allometrically-scaled PP (p = <0.001 to 0.026, ES = 1.06 to 2.01). Weaker and less powerful soccer players can benefit from strength-focused training to improve loaded SJ kinetics associating with force production.

Acute Effects of Match-Play on Neuromuscular and Subjective Recovery and Stress State in Division I Collegiate Female Soccer Players.

ABSTRACT: Ishida, A, Bazyler, CD, Sayers, AL, Mizuguchi, S, and Gentles, JA. Acute effects of match-play on neuromuscular and subjective recovery and stress state in Division I collegiate female soccer players. J Strength Cond Res 35(4): 976-982, 2021-The purpose of this study was to investigate acute effects of match-play on neuromuscular performance and subjective recovery and stress state and the relationship between training load (TL) and changes in neuromuscular performance in female soccer players. Twelve National Collegiate Athlete Association Division I players participated (20.7 ± 2.3 years; 64.4 ± 7.2 kg; 164.5 ± 6.0 cm) and completed countermovement jump (CMJ) at 0 kg (CMJ0) and 20 kg (CMJ20) and the Short Recovery Stress Scale (SRSS) at 3 hours pre-match (Pre), 12 hours post-match (Post12), and 38 hours post-match (Post38). Countermovement jump variables included body mass, jump height (JH), modified reactive strength index (RSI), peak force (PF), relative PF, eccentric impulse, concentric impulse (CI), peak power (PP), relative PP (RPP), eccentric average PP, and concentric average power (CAP). The SRSS consists of 4 Stress Scales (SSs) and 4 Recovery Scales (RSs). Training loads included total distance, total PlayerLoad, high-speed running, and session ratings of perceived exertion. Significant moderate to large decreases were observed from Pre to Post12 in JH, RSI, CI, PP, RPP, and CAP in CMJ0 and CMJ20 (p < 0.05, effect size [ES] = 0.63-1.35). Significant changes were observed from Pre to Post12 in all RSs (p < 0.05, ES = 0.65-0.79) and 3 SSs (p < 0.05, ES = 0.71-0.77). Significant correlations were observed between CMJ20 PP from Pre to Post12 and all TLs (p < 0.05, r = -0.58 to -0.68). CMJ0 and CMJ20 JH and PP may indicate acute neuromuscular changes after match-play. The magnitude of CMJ20 PP decrements from Pre to Post12 may be affected by soccer match-play volumes.

Associations of Body Composition, Maximum Strength, Power Characteristics with Sprinting, Jumping, and Intermittent Endurance Performance in Male Intercollegiate Soccer Players.

The purpose of this study was to determine the relationships between body composition, strength, power characteristics, sprinting, jumping, and intermittent endurance performance in collegiate male players. Twenty-three players participated (19.7 ± 1.6 yrs; 71.8 ± 7.1 kg; 176.5 ± 5.1 cm). Measurements of interest in body composition included body fat percentage (BF%), lean body mass (LBM), and body mass (BM). Power characteristics were measured with an unloaded squat jump (SJ0) and loaded SJ at 20 kg (SJ20) and 40 kg (SJ40), and unloaded countermovement jump (CMJ0). Power assessments included peak power (PP) and PP allometrically scaled (PPa). Strength characteristics were assessed using isometric mid-thigh pull. Strength assessment included isometric peak force (IPF) and IPF allometrically scaled (IPFa). Performance measures included 10m and 20 m sprint time, CMJ0 jump-height, and Yo-Yo intermittent endurance test level 1 distance. Significant correlations ranging from moderate to very large were found for LBM and CMJ jump height (CM0 JH) (p = 0.01, r = 0.50); BF% and sprint times at 10 m (p = 0.03, r = 0.44) and 20 m (p = 0.02, r = 0.50). PP and PPa from SJ0 and CMJ0 were significantly correlated to 10m sprint time (p < 0.05, r = -0.45 to -0.53) and 20 m sprint time (p < 0.05, r = -0.40 to -0.49). Our findings agree with previous literature in that body composition and power characteristics are directly related to soccer-related performance.

Effects of an Initial Muscle Strength Level on Sports Performance Changes in Collegiate Soccer Players.

The purposes of this study were to investigate effects of partial block periodized strength training on physical performance and to examine relationships between initial muscle strength measured with isometric mid-thigh pull (IMTP) and performance changes after 7 weeks of strength training. Seventeen collegiate male soccer players participated. Initial muscle strength was determined using IMTP while physical performance included 10 m and 20 m sprints and static vertical jump with a polyvinyl chloride pipe (SJ0), 20 kg barbell (SJ20), and barbell loaded to 40 kg bar (SJ40). Performance testing was performed at three points: before first week (baseline), fourth week (T1), and seventh week (T2). Statistically small to moderate changes were found from baseline to T2 in peak power (PP; p < 0.001, ES = 0.49), net impulse (NI; p < 0.001, ES = 0.49), peak velocity (PV; p < 0.001, ES = 0.62), allometrically scaled PP (PPa; p < 0.001, ES = 0.62) in SJ20 and jump height (JH) in SJ40 (p < 0.001, ES = 0.36). Moderate to large correlations were found between isometric peak force and the changes from baseline to T2 in SJ20 PP (p = 0.04, r = -0.49), SJ20 PF (p = 0.03, r = -0.52), PPa (p = 0.04, r = -0.50), and SJ20 allometrically scaled peak force (p = 0.04, r = -0.49). Properly structured strength training maximizes task-specific physical performance. Initial muscle strength negatively affects the magnitudes of adaptations to physical performance.

Emphasizing Task-Specific Hypertrophy to Enhance Sequential Strength and Power Performance.

While strength is indeed a skill, most discussions have primarily considered structural adaptations rather than ultrastructural augmentation to improve performance. Altering the structural component of the muscle is often the aim of hypertrophic training, yet not all hypertrophy is equal; such alterations are dependent upon how the muscle adapts to the training stimuli and overall training stress. When comparing bodybuilders to strength and power athletes such as powerlifters, weightlifters, and throwers, while muscle size may be similar, the ability to produce force and power is often inequivalent. Thus, performance differences go beyond structural changes and may be due to the muscle's ultrastructural constituents and training induced adaptations. Relative to potentiating strength and power performances, eliciting specific ultrastructural changes should be a variable of interest during hypertrophic training phases. By focusing on task-specific hypertrophy, it may be possible to achieve an optimal amount of hypertrophy while deemphasizing metabolic and aerobic components that are often associated with high-volume training. Therefore, the purpose of this article is to briefly address different types of hypertrophy and provide directions for practitioners who are aiming to achieve optimal rather than maximal hypertrophy, as it relates to altering ultrastructural muscular components, to potentiate strength and power performance.

Effects of bilateral cochlear implants in children: Timing of second surgery and the significance of wearing bilateral cochlear implants in Japan.

OBJECTIVE: This study aims to evaluate the speech perception with first, second, or bilateral cochlear implants (CI) and to reveal the effects of wearing bilateral CI in children. METHODS: After reviewing the medical records, a total of 19 children who underwent bilateral cochlear implantation serially between 2012 and 2015 at Kyoto University Hospital (tertiary referral center) were included in this study. All patients had no delay in language development. The study group comprised nine boys and ten girls, and their age ranged from 3 years 8 months to 12 years 5 months when they underwent the tests in this study. The mean and median ages were 8 years 6 months and 9 years 2 months, respectively. We measured the appropriate signal/noise ratio (SNR) to test speech perception of Japanese language in noise by testing the hearing ability of unilateral CI patients with or without noise and by surveying the sound environment in a classroom of a mainstream elementary school. Speech perception in quiet and noise and the left-right localization ability were examined using first, second, or bilateral cochlear implants in all patients. RESULTS: Considering the results of hearing ability tests with noise and the SNR of the elementary school classrooms, we decided to use SNR of +10 dB to evaluate the speech perception ability in noise. The speech perception ability using the second CI was significantly worse in patients undergoing second cochlear implantation after 7 years old than in those who underwent surgery before 3.5 years old. Moreover, patients undergoing second cochlear implantation before 7 years old showed significantly better left-right localization of high-frequency sound. CONCLUSIONS: Second cochlear implantation before 7 years old is a critical factor in acquiring beneficial speech perception ability with the second CI and sound localization ability with the bilateral CI.

Coptidis Rhizoma: protective effects against peroxynitrite-induced oxidative damage and elucidation of its active components.

We have investigated the protective effects of Coptidis Rhizoma against peroxynitrite (ONOO(-))-induced oxidative damage and have elucidated the active components of this preparation. In an in-vitro system, Coptidis Rhizoma extract scavenged ONOO(-) and its precursors, nitric oxide (NO) and superoxide anion (O(2)(-)). This scavenging activity was more marked for ONOO(-) than its precursors. In addition, against 3-morpholinosydnonimine-induced cellular damage, this extract significantly reduced cellular ONOO(-) formation and increased cell viability. In an in-vivo lipopolysaccharide plus ischaemia-reperfusion system that generated ONOO(-), the administration of Coptidis Rhizoma extract at 50 and 100 mg kg(-1)/day for 30 days exerted greater inhibition of ONOO(-) than NO and O(2)(-). This suggested that it acted as a direct scavenger of ONOO(-) rather than as a scavenger of its precursors. Moreover, the suppression of the activities of the antioxidative enzymes superoxide dismutase, catalase and glutathione peroxidase was significantly attenuated by the administration of Coptidis Rhizoma extract. Furthermore, the extract ameliorated renal dysfunction judged by decreasing serum urea nitrogen and creatinine levels. To elucidate the active components of Coptidis Rhizoma extract, we evaluated and compared the effects of the phenol plus alkaloid and alkaloid fractions on ONOO-induced damage. We found that the alkaloid fraction consisting of berberine, palmatine and coptisine was the most effective at protecting against ONOO(-). We confirmed that berberine (10 and 20 mg kg(-1)/day for 10 days), the main and most active alkaloid in Coptidis Rhizoma extract, was also protective, exerting NO-, O(2)(-)- and ONOO(-)-scavenging activities. This study suggested that Coptidis Rhizoma could protect against ONOO(-)-induced oxidative damage and that this effect was mainly attributable to the constituent alkaloids, especially berberine. This study is the first to demonstrate an antioxidative effect of alkaloids, including berberine, against ONOO(-)-induced damage.