ABSTRACT
Background: Gross motor coordination (GMC) plays a crucial factor in children's motor development and daily activities. It encompasses various sub-capacities, such as spatial orientation, rhythm, and motor reaction, collectively referred to as basic coordination capacities (BCC). However, children who are overweight and obese (OW/OB) often display poorer GMC. This study aims to examine the impact of gender and weight status (BMI categories) on children's GMC and BCC. It also seeks to investigate the impact of BCC and BMI on GMC. Method: The study involved 266 participants, 135 in the NW group (boys: n = 75; girls: n = 60) and 131 in the OW/OB group (boys: n = 68; girls: n = 63). An NW status is defined by a BMI z-score between ≥-2SD to ≤1SD, while an OW/OB status corresponds to a BMI z-score > 1SD. Physical activity was assessed using the Physical Activity Questionnaire for Children, developed by the University of Saskatchewan, Canada. We used six field tests to evaluate BCC, including single leg standing test (static balance), YBT (dynamic balance), rhythmic sprint test (rhythm), reaction time test (motor reaction), target standing broad test (kinesthetic differentiation), and numbered medicine ball running test (spatial orientation). GMC was evaluated with Kiphard-Schilling's Body Coordination Test (KTK). Result: The motor quotient (MQ) was primarily affected by weight status (F = 516.599, p < 0.001; gender: F = 6.694, p = 0.01), with no significant interaction effect (F = 0.062, p = 0.803). In BCC, gender had a significant main effect on rhythm capacity (F = 29.611, p < 0.001) and static balance (F = 11.257, p = 0.001) but did not significant influence other sub-capacities (p > 0.05). Weight status impacted dynamic balance (F = 11.164, p = 0.001). The interaction of gender and weight status significantly impacted motor reaction (F = 1.471, p = 0.024) and kinesthetic differentiation (F = 5.454, p = 0.02), but did not affect other sub-capacities (p > 0.05). The physical activity was not significant affected by gender (F = 0.099, p = 0.753), weight status (F = 0.171, p = 0.679) and the interactions of two variables (F = 0.06, p = 0.806). In the regression analysis, except motor reaction (p > 0.05), other BCC sub-capacities influenced GMC to varying extents (ß = -0.103-0.189, p < 0.05). Nonetheless, only two types of balance significantly mediated the relationship between BMI and GMC (BMIâMQ: ß = -0.543, p < 0.001; BMIâYBT: ß = -0.315, p < 0.001; BMIâSLS: ß = -0.282, p < 0.001; SLSâMQ: ß = 0.189, p < 0.001; YBTâMQ: ß = 0.182, p < 0.001). Conclusion: Compared to gender, the main effect of weight status on most GMC and BCC's sub-capacities was more pronounced. OW/OB children exhibited poorer GMC, which is related to their reduced static and dynamic balance due to excess weight. Kinesthetic differentiation, spatial orientation, and rhythm capacity are not significantly associated with BMI, but these sub-capacities positively influence gross motor coordination (GMC), except for hand-eye motor reaction.
Subject(s)
Body Mass Index , Motor Skills , Humans , Male , Female , Child , Motor Skills/physiology , Pediatric Obesity/physiopathology , Pediatric Obesity/epidemiology , Overweight/physiopathology , Overweight/epidemiology , Postural Balance/physiology , Exercise/physiology , Reaction Time/physiology , Psychomotor Performance/physiologyABSTRACT
Electrocatalysis is a crucial method for achieving global carbon neutrality, serving as an essential means of energy conversion, and electrocatalyst is crucial in the process of electrocatalysis. Because of the abundant active sites, the multi-component synergistic effect of high-entropy materials has a wide application prospect in the field of electrocatalysis. Moreover, due to the special structure of high-entropy materials, it is possible to obtain almost continuous adsorption energy distribution by regulating the composition, which has attracted extensive attention of researchers. This paper reviews the properties and types of high-entropy materials, including alloys and compounds. The synthesis strategies of high-entropy materials are systematically introduced, and the solid phase synthesis, liquid-phase synthesis, and gas-phase synthesis are classified and summarized. The application of high-entropy materials in electrocatalysis is summarized, and the promotion effect of high-entropy strategy in various catalytic reaction processes is summarized. Finally, the current progress of high-entropy materials, the problems encountered, and the future development direction are reviewed. It is emphasized that the strategy of high flux density functional theory calculation guiding high-entropy catalyst design will be of great significance to electrocatalysis.
ABSTRACT
Despite accumulating evidence that blood flow restriction (BFR) training promotes muscle hypertrophy and strength gain, the underlying neurophysiological mechanisms have rarely been explored. The primary goal of this study is to investigate characteristics of cerebral cortex activity during BFR training under different pressure intensities. 24 males participated in 30% 1RM squat exercise, changes in oxygenated hemoglobin concentration (HbO) in the primary motor cortex (M1), pre-motor cortex (PMC), supplementary motor area (SMA), and dorsolateral prefrontal cortex (DLPFC), were measured by fNIRS. The results showed that HbO increased from 0 mmHg (non-BFR) to 250 mmHg but dropped sharply under 350 mmHg pressure intensity. In addition, HbO and functional connectivity were higher in M1 and PMC-SMA than in DLPFC. Moreover, the significant interaction effect between pressure intensity and ROI for HbO revealed that the regulation of cerebral cortex during BFR training was more pronounced in M1 and PMC-SMA than in DLPFC. In conclusion, low-load resistance training with BFR triggers acute responses in the cerebral cortex, and moderate pressure intensity achieves optimal neural benefits in enhancing cortical activation. M1 and PMC-SMA play crucial roles during BFR training through activation and functional connectivity regulation.
Subject(s)
Cerebral Cortex , Motor Cortex , Resistance Training , Spectroscopy, Near-Infrared , Humans , Male , Resistance Training/methods , Young Adult , Cerebral Cortex/physiology , Cerebral Cortex/blood supply , Cerebral Cortex/metabolism , Cerebral Cortex/diagnostic imaging , Spectroscopy, Near-Infrared/methods , Adult , Motor Cortex/physiology , Motor Cortex/diagnostic imaging , Prefrontal Cortex/physiology , Prefrontal Cortex/blood supply , Prefrontal Cortex/metabolism , Prefrontal Cortex/diagnostic imagingABSTRACT
Room-temperature sodium-sulfur (RT Na-S) batteries, noted for their low material costs and high energy density, are emerging as a promising alternative to lithium-ion batteries (LIBs) in various applications including power grids and standalone renewable energy systems. These batteries are commonly assembled with glass fiber membranes, which face significant challenges like the dissolution of polysulfides, sluggish sulfur conversion kinetics, and the growth of Na dendrites. Here, we develop an amorphous two-dimensional (2D) iron tin oxide (A-FeSnOx) nanosheet with hierarchical vacancies, including abundant oxygen vacancies (Ovs) and nano-sized perforations, that can be assembled into a multifunctional layer overlaying commercial separators for RT Na-S batteries. The Ovs offer strong adsorption and abundant catalytic sites for polysulfides, while the defect concentration is finely tuned to elucidate the polysulfides conversion mechanisms. The nano-sized perforations aid in regulating Na ions transport, resulting in uniform Na deposition. Moreover, the strategic addition of trace amounts of Ti3C2 (MXene) forms an amorphous/crystalline (A/C) interface that significantly improves the mechanical properties of the separator and suppresses dendrite growth. As a result, the task-specific layer achieves ultra-light (~0.1â mg cm-2), ultra-thin (~200â nm), and ultra-robust (modulus=4.9â GPa) characteristics. Consequently, the RT Na-S battery maintained a high capacity of 610.3â mAh g-1 and an average Coulombic efficiency of 99.9 % after 400â cycles at 0.5â C.
ABSTRACT
In recent years, aggregation-induced emission luminogens (AIEgens) have witnessed numerous groundbreaking advances in fundamental theoretical research and functional applications. Notably, stimuli-responsive AIEgens have achieved remarkable results, demonstrating immense potential for application in various fields such as chemistry, materials science, biology, and medicine. Herein, two multi-stimuli-responsive cyanostilbene derivatives TPE-CNTPA and PH-CNTPA were synthesized by introducing tetraphenylethylene (TPE) and trifluoromethyl groups, respectively. Primarily, under the combined mechanism of aggregation-induced emission (AIE) and twisted intramolecular charge transfer (TICT), TPE-CNTPA and PH-CNTPA exhibit "on-off-on" fluorescent emission characteristics in solution. Secondly, under 365 nm ultraviolet light irradiation, the photo-induced isomerization of PH-CNTPA causes changes in photophysical property, demonstrating its responsiveness to ultraviolet light. In addition, TPE-CNTPA and PH-CNTPA exhibit high-contrast mechanochromic properties, providing broader possibilities for their potential applications in various fields. Moreover, owing to the unique fluorescence emission characteristics, TPE-CNTPA and PH-CNTP have enormous potential for application in the field of encryption anti-counterfeiting. Besides, PH-CNTPA can be utilized for the detection of trace water in single or mixed solvents, demonstrating outstanding sensitivity and anti-interference properties in different solvents. This research work reveals the potential in the fields of water sensing and anti-counterfeiting for these two multi-stimuli-responsive compounds.
ABSTRACT
This study compared the therapeutic effect and safety between warfarin anticoagulation and percutaneous left atrial appendage transcatheter occlusion (PLAATO) in non-valvular atrial fibrillation (NVAF). A total of 110 patients were selected and assigned to Control group (n=55) and Observation group (n=55). The control patients were used warfarin, while the observation patients were performed PLAATO. The coagulation function, stroke and bleeding scores were compared between the two groups at different times. Left ventricular function before therapy and 1 year after therapy and adverse events during follow-up were compared between the two groups. After one month of treatment, CHA2DS2-VASC, HAS-BLED score, serum ET-1 and hs-CRP levels were lower in the PLAATO patients than in warfarin patients, but serum PDGFs levels were higher than patients in the warfarin patients (P < 0.05). One month after treatment, the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) of the PLAATO patients was longer than that of the warfarin patients (P < 0.05), but the levels of fibrinogen (FIB) in the PLAATO patients were lower than that of the warfarin patients (P < 0.05). In addition, one year after therapy, the left atrial end-diastolic volume (LAEDV), left atrial end-systolic volume (LAESV) and left atrial inner diameter of the two groups were significantly reduced (P < 0.05). Left atrial appendage (LAA) occlusion can effectively improve the cardiac function and coagulation function of NVAF patients, with lower incidence of bleeding events, stroke events and higher safety.
Subject(s)
Anticoagulants , Atrial Fibrillation , Blood Coagulation , Left Atrial Appendage Closure , Warfarin , Warfarin/adverse effects , Warfarin/pharmacology , Anticoagulants/adverse effects , Anticoagulants/pharmacology , Atrial Fibrillation/blood , Atrial Fibrillation/therapy , Atrial Appendage/physiopathology , Stroke/etiology , Humans , Male , Female , Middle Aged , Aged , Hemorrhage/etiology , Partial Thromboplastin Time , Prothrombin Time , Thrombin Time , Blood Coagulation/drug effects , Endothelin-1/blood , C-Reactive Protein/analysisABSTRACT
Metal hexacyanoferrates (HCFs) are viewed as promising cathode materials for potassium-ion batteries (PIBs) because of their high theoretical capacities and redox potentials. However, the development of an HCF cathode with high cycling stability and voltage retention is still impeded by the unavoidable Fe(CN)6 vacancies (VFeCN) and H2O in the materials. Here, a repair method is proposed that significantly reduces the VFeCN content in potassium manganese hexacyanoferrate (KMHCF) enabled by the reducibility of sodium citrate and removal of ligand H2O at high temperature (KMHCF-H). The KMHCF-H obtained at 90 °C contains only 2% VFeCN, and the VFeCN is concentrated in the lattice interior. Such an integrated Fe-CN-Mn surface structure of the KMHCF-H cathode with repaired surface VFeCN allows preferential decomposition of potassium bis(fluorosulfonyl)imide (KFSI) in the electrolyte, which constitutes a dense anion-dominated cathode electrolyte interphase (CEI) , inhibiting effectively Mn dissolution into the electrolyte. Consequently, the KMHCF-H cathode exhibits excellent cycling performance for both half-cell (95.2 % at 0.2 Ag-1 after 2000 cycles) and full-cell (99.4 % at 0.1 Ag-1 after 200 cycles). This thermal repair method enables scalable preparation of KMHCF with a low content of vacancies, holding substantial promise for practical applications of PIBs.
ABSTRACT
The electrochemical carbon dioxide reduction reaction (CO2 RR) to formate is of great interest in the field of electrochemical energy. Cu-based material is an appealing electrocatalyst for the CO2 RR. However, retaining Cu2+ under the high cathodic potential of CO2 RR remains a great challenge, leading to low electrocatalytic selectivity, activity, and stability. Herein, inspired by corrosion science, a sacrificial protection strategy to stabilize interfacial crystalline CuO through embedding of active amorphous SnO2 (c-CuO/a-SnO2 ) is reported, which greatly boosts the electrocatalytic sensitivity, activity, and stability for CO2 RR to formate. The as-made hybrid catalyst can achieve superior high selectivity for CO2 RR to formate with a remarkable Faradaic efficiency (FE) of 96.7%, and a superhigh current density of over 1 A cm-2 that far outperforms industrial benchmarks (FE > 90%, current density > 300 mA cm-2 ). In situ X-ray absorption spectroscopy (XAS) and X-ray diffractionexperimental and theoretical calculation results reveal that the broadened s-orbital in interfacial a-SnO2 offers the lower orbital for extra electrons than Cu2+ , which can effectively retain nearby Cu2+ , and the high active interface significantly lowers the energy barrier of the limited step (* CO2 â * HCOO) and enhances the selectivity and activity for CO2 RR to formate.
ABSTRACT
Partial multi-label learning (PML) is an emerging weakly supervised learning framework, where each training example is associated with multiple candidate labels which are only partially valid. To learn the multi-label predictive model from PML training examples, most existing approaches work by identifying valid labels within candidate label set via label confidence estimation. In this paper, a novel strategy towards partial multi-label learning is proposed by enabling binary decomposition for handling PML training examples. Specifically, the widely used error-correcting output codes (ECOC) techniques are adapted to transform the PML learning problem into a number of binary learning problems, which refrains from using the error-prone procedure of estimating labeling confidence of individual candidate label. In the encoding phase, a ternary encoding scheme is utilized to balance the definiteness and adequacy of the derived binary training set. In the decoding phase, a loss weighted scheme is applied to consider the empirical performance and predictive margin of derived binary classifiers. Extensive comparative studies against state-of-the-art PML learning approaches clearly show the performance advantage of the proposed binary decomposition strategy for partial multi-label learning.
ABSTRACT
Based on the classical Schiff base reaction, fluorescent probe dimethyl 5-((pyren-1-ylmethylene)amino)isophthalate (PAI) is designed and synthesized through introducing Schiff base structure to pyrene unit for structural modification. The structure of the synthesized probe PAI is determined and characterized by FT-IR, 1H NMR, 13C NMR and HRMS. PAI is a type of "turn-on" probe which can specifically recognize Al3+ ion with high selectivity. The limit of detection is calculated to be 3.07 × 10-8 M, which proves the probe's high sensitivity and is lower than that of many efficient reported probes. The probe PAI is intrinsically non-fluorescent due to the photoinduced electron transfer (PET) process. However, the addition of Al3+ ion leads to the breakage of the carbon-nitrogen double bond of Schiff base in PAI resulting in the product without PET property, which shows a typical localized state with enhanced fluorescence and blue color. In addition, PAI can recognize Al3+ ion through test papers, which is in favor of the future research regarding to Al3+ ion sensing.
Subject(s)
Fluorescent Dyes , Schiff Bases , Fluorescent Dyes/chemistry , Schiff Bases/chemistry , Spectroscopy, Fourier Transform Infrared , Pyrenes/chemistry , Spectrometry, Fluorescence/methodsSubject(s)
Elasticity Imaging Techniques , Hepatitis B , Hypertension, Portal , Humans , Spleen/diagnostic imaging , Spleen/pathology , Hardness , Liver/diagnostic imaging , Liver/pathology , Liver Cirrhosis/complications , Liver Cirrhosis/diagnostic imaging , Hypertension, Portal/diagnostic imaging , Hypertension, Portal/etiology , Hepatitis B/complications , Hepatitis B/diagnosisABSTRACT
Graphene oxide (GO) and reduced GO possess robust mechanical, electrical and chemical properties. Their nanocomposites have been extensively explored for applications in diverse fields. However, due to the high flexibility and weak interlayer interactions of GO nanosheets, the flexural mechanical properties of GO-based composites, especially in bulk materials, are largely constrained, which hinders their performance in practical applications. Here, inspired by the amorphous/crystalline feature of the heterophase within nacreous platelets, we present a centimetre-sized, GO-based bulk material consisting of building blocks of GO and amorphous/crystalline leaf-like MnO2 hexagon nanosheets adhered together with polymer-based crosslinkers. These building blocks are stacked and hot-pressed with further crosslinking between the layers to form a GO/MnO2-based layered (GML) bulk material. The resultant GML bulk material exhibits a flexural strength of 231.2 MPa. Moreover, the material exhibits sufficient fracture toughness and strong impact resistance while being light in weight. Experimental and numerical analyses indicate that the ordered heterophase structure and synergetic crosslinking interactions across multiscale interfaces lead to the superior mechanical properties of the material. These results are expected to provide insights into the design of structural materials and potential applications of high-performance GO-based bulk materials in aerospace, biomedicine and electronics.
Subject(s)
Graphite , Oxides , Graphite/chemistry , Manganese Compounds , Oxides/chemistry , Polymers/chemistryABSTRACT
Multi-dimensional classification (MDC) assumes heterogeneous class spaces for each example, where class variables from different class spaces characterize semantics of the example along different dimensions. The heterogeneity of class spaces leads to incomparability of the modeling outputs from different class spaces, which is the major difficulty in designing MDC approaches. In this article, we make a first attempt toward adapting maximum margin techniques for MDC problem and a novel approach named M3MDC is proposed. Specifically, M3MDC maximizes the margins between each pair of class labels with respect to individual class variable while modeling relationship across class variables (as well as class labels within individual class variable) via covariance regularization. The resulting formulation admits convex objective function with nonlinear constraints, which can be solved via alternating optimization with quadratic programming (QP) or closed-form solution in either alternating step. Comparative studies on the most comprehensive real-world MDC datasets to date are conducted and it is shown that M3MDC achieves highly competitive performance against state-of-the-art MDC approaches.
ABSTRACT
Low-carbon society is calling for advanced electrochemical energy storage and conversion systems and techniques, in which functional electrode materials are a core factor. As a new member of the material family, two-dimensional amorphous nanomaterials (2D ANMs) are booming gradually and show promising application prospects in electrochemical fields for extended specific surface area, abundant active sites, tunable electron states, and faster ion transport capacity. Specifically, their flexible structures provide significant adjustment room that allows readily and desirable modification. Recent advances have witnessed omnifarious manipulation means on 2D ANMs for enhanced electrochemical performance. Here, this review is devoted to collecting and summarizing the manipulation strategies of 2D ANMs in terms of component interaction and geometric configuration design, expecting to promote the controllable development of such a new class of nanomaterial. Our view covers the 2D ANMs applied in electrochemical fields, including battery, supercapacitor, and electrocatalysis, meanwhile we also clarify the relationship between manipulation manner and beneficial effect on electrochemical properties. Finally, we conclude the review with our personal insights and provide an outlook for more effective manipulation ways on functional and practical 2D ANMs.
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BACKGROUND: Duration perception is an essential part of our cognitive and behavioral system, helping us interact with the outside world. An integrated model of timing, which states that the perceived duration of a given stimulus is based on the efficiency of information extraction, was recently set forth to improve current understanding of the representation and judgment of time. However, the prediction from this model that more efficient information extraction results in longer perceived duration has not been tested. Thus, the aim of this study is to investigate whether sports experts, as a group of individuals with information extraction superiority in situations relevant to their sport skill, have longer duration perceptions when they view expertise-related stimuli compared with others with no expertise/experience. METHODS: For this study, 81 subjects were recruited based on a prior power analysis. The sports experts group had 27 athletes with years of professional training in diving; a wrestler group and a nonathlete group, with each of these groups having 27 subjects, were used as controls. All participants completed a classic duration reproduction task for subsecond and suprasecond durations with both the diving images and general images involved. RESULTS: The divers reproduced longer durations for diving stimuli compared with general stimuli under both subsecond and suprasecond time ranges, while the other samples showed the opposite pattern. Furthermore, the years of training in diving were positively correlated with the magnitude of the prolonged reproduction duration when divers viewed diving stimuli. Moreover, the diver group showed a more precise duration perception in subsecond time range for general stimuli compared with the wrestlers and nonathletes. CONCLUSION: The results suggest that sports experts perceive longer duration when viewing expertise-related stimuli compared with others with no expertise/experience.
ABSTRACT
The leaf-like structure is a classic and robust structure and its unique vein support can reduce structural instability. However, biomimetic leaf structures on the atomic scale are rarely reported due to the difficulty in achieving a stable vein-like support in a mesophyll-like substrate. A breathable 2D MnO2 artificial leaf is first reported with atomic thickness by using a simple and mild one-step wet chemical method. This homogeneous ultrathin leaf-like structure comprises of vein-like crystalline skeleton as support and amorphous microporous mesophyll-like nanosheet as substrate. When used as an anode material for lithium ion batteries, it first solves the irreversible capacity loss and poor cycling issue of pure MnO2 , which delivers high capacity of 1210 mAh g-1 at 0.1 A g-1 and extremely stable cycle life over 2500 cycles at 1.0 A g-1 . It exhibits the most outstanding cycle life of pure MnO2 and even comparable to the most MnO2 -based composite electrode materials. This biomimetic design provides important guidelines for precise control of 2D artificial systems and gives a new idea for solving poor electrochemical stability of pure metal oxide electrode materials.
ABSTRACT
Improving electro-optic properties is essential for fabricating high-quality liquid crystal displays. Herein, by doping amorphous Mn3 O4 octahedral nanocages (a-Mn3 O4 ONCs) into a nematic liquid crystal (NLC) matrix E7, outstanding electro-optic properties of the blend are successfully obtained. At a doping concentration of 0.03 wt%, the maximum decreases of threshold voltage (Vth ) and saturation voltage (Vsat ) are 34% and 31%, respectively, and the increase of contrast (Con ) is 160%. This remarkable electro-optic activity can be attributed to high-efficiency charge transfer within the a-Mn3 O4 ONCs NLC system, caused by metastable electronic states of a-Mn3 O4 ONCs. To the best of our knowledge, such remarkable decreased electro-optic activity is observed for the first time from doping amorphous semiconductors, which could provide a new pathway to develop excellent energy-saving amorphous materials and improve their potential applications in electro-optical devices.
ABSTRACT
It is well established that motor expertise is linked to superior mental rotation ability, but few studies have attempted to explain the factors that influence the stages of mental rotation in sport experts. Some authors have argued that athletes are faster in the perceptual and decision stages but not in the rotation stages of object-based transformations; however, stimuli related to sport have not been used to test mental rotation with egocentric transformations. Therefore, 24 adolescent elite divers and 23 adolescent nonathletes completed mental rotation tasks with object-based and egocentric transformations. The results showed faster reaction times (RTs) for the motor experts in tasks with both types of transformations (object-based cube, object-based body, and egocentric body). Additionally, the differences in favour of motor experts in the perceptual and decision stages were confirmed. Interestingly, motor experts also outperformed nonathletes in the rotation stages in the egocentric transformations. These findings are discussed against the background of the effects of sport expertise on mental rotation.
ABSTRACT
Dendritic cells (DCs) are potent and specialized antigen-presenting cells that play a crucial role in initiating and amplifying both the innate and adaptive immune responses. Tumor cells can escape from immune attack by secreting suppressive cytokines which solely or cooperatively impair the immune function and microrheological properties of DCs. However, the underlying mechanisms are not fully defined. Transforming growth factor-ß1 (TGF-ß1) has been identified as a major cytokine in the tumor microenvironment. To determine the effects of TGF-ß1 on mature DCs (mDCs) from microrheological viewpoint, cells were treated with different concentrations of TGF-ß1. The results showed that the impaired microrheological parameters, including osmotic fragility, electrophoretic mobility, deformability, membrane fluidity, F-actin organization and so on, as well as motilities of mDCs relied heavily on TGF-ß1 concentration. Moreover, these changes were correlated with the expression levels of fascin1, cofilin1, phosphorylated cofilin1 and profilin, this could be one of the crucial aspects of immune escape mechanisms of tumors, hinting that the signal pathway of TGF-ß1 should be blocked in appropriate way before performing DCs-based immunotherapy against cancer. It is clinically important to understand the biological behavior of DCs and immune escape mechanism of tumor as well as how to improve efficiency of the anti-tumor therapy based on DCs.
Subject(s)
Dendritic Cells/drug effects , Transforming Growth Factor beta1/pharmacology , Actins/metabolism , Antigens, CD/blood , Apoptosis/drug effects , Dendritic Cells/cytology , Dendritic Cells/metabolism , Human Umbilical Vein Endothelial Cells , Humans , Linear Models , Microscopy, Confocal , Osmotic Fragility , Rheology/methods , Viscoelastic Substances/chemistryABSTRACT
Contrast media are the commonly used agents in radiology. However, because of their characteristics of high osmolality, high viscosity, and chemical toxicity, the administrations of contrast media have been shown to cause adverse effects especially on hemorheology in short time course. The present study is to find the effects of a non-ionic contrast medium, iopromide, on the hemorheology in long time course both in vitro and in vivo. For in vitro treatment, human peripheral blood samples were incubated with contrast medium at 37°C for 0.5, 1 and 2 h. For in vivo study, about 15 ml of contrast medium was injected into rabbits and blood samples were collected at 0.5, 2, 6, and 24 h after the bolus injection. Hemorheological parameters were examined. Results showed that hematocrit adjusted whole blood viscosity increased significantly at 1 h after in vitro treatment of contrast medium, while it decreased at 0.5 h and remained low till 6 h after bolus injection. Ektacytometer showed that erythrocyte deformability decreased to the lowest level at 2 h in vitro and it dropped at 0.5 h and resumed to normal after 2 h in vivo. Erythrocyte small deformation indices were reduced by contrast medium in both in vitro and in vivo studies. Erythrocyte orientation index was also reduced in in vivo study. Erythrocyte electrophoresis rates at all time points decreased but osmotic fragility did not change in both studies. These impaired hemorheological parameters may disturb the microcirculation and cause adverse effects in patients with kidney diseases.