In silico epitope prediction and evolutionary analysis reveals capsid mutation patterns for enterovirus B.

Enterovirus B (EVB) is a common species of enterovirus, mainly consisting of Echovirus (Echo) and Coxsackievirus B (CVB). The population is generally susceptible to EVB, especially among children. Since the 21st century, EVB has been widely prevalent worldwide, and can cause serious diseases, such as viral meningitis, myocarditis, and neonatal sepsis. By using cryo-electron microscopy, the three-dimensional (3D) structures of EVB and their uncoating receptors (FcRn and CAR) have been determined, laying the foundation for the study of viral pathogenesis and therapeutic antibodies. A limited number of epitopes bound to neutralizing antibodies have also been determined. It is unclear whether additional epitopes are present or whether epitope mutations play a key role in molecular evolutionary history and epidemics, as in influenza and SARS-CoV-2. In the current study, the conformational epitopes of six representative EVB serotypes (E6, E11, E30, CVB1, CVB3 and CVB5) were systematically predicted by bioinformatics-based epitope prediction algorithm. We found that their epitopes were distributed into three clusters, where the VP1 BC loop, C-terminus and VP2 EF loop were the main regions of EVB epitopes. Among them, the VP1 BC loop and VP2 EF loop may be the key epitope regions that determined the use of the uncoating receptors. Further molecular evolution analysis based on the VP1 and genome sequences showed that the VP1 C-terminus and VP2 EF loop, as well as a potential "breathing epitope" VP1 N-terminus, were common mutation hotspot regions, suggesting that the emergence of evolutionary clades was driven by epitope mutations. Finally, footprints showed mutations were located on or near epitopes, while mutations on the receptor binding sites were rare. This suggested that EVB promotes viral epidemics by breaking the immune barrier through epitope mutations, but the mutations avoided the receptor binding sites. The bioinformatics study of EVB epitopes may provide important information for the monitoring and early warning of EVB epidemics and developing therapeutic antibodies.

Antenna/Propagation Domain Self-Interference Cancellation (SIC) for In-Band Full-Duplex Wireless Communication Systems.

In-band full duplex (IBFD) is regarded as one of the most significant technologies for addressing the issue of spectrum scarcity in 5G and beyond systems. In the realization of practical IBFD systems, self-interference, i.e., the interference that the transmitter causes to the collocated receiver, poses a major challenge to antenna designers; it is a prerequisite for applying other self-interference cancellation (SIC) techniques in the analog and digital domains. In this paper, a comprehensive survey on SIC techniques in the antenna/propagation (AP) domain is provided and the pros and cons of each technique are studied. Opportunities and challenges of employing IBFD antennas in future wireless communications networks are discussed.