ABSTRACT
Early initiation of therapy in patients with Alport syndrome (AS) slows down renal failure by many years. Genotype-phenotype correlations propose that the location and character of the individual's variant correlate with the renal outcome and any extra renal manifestations. In-depth clinical and genetic data of 60/62 children who participated in the EARLY PRO-TECT Alport trial were analyzed. Genetic variants were interpreted according to current guidelines and criteria. Genetically solved patients with X-linked inheritance were then classified according to the severity of their COL4A5 variant into less-severe, intermediate, and severe groups and disease progress was compared. Almost 90% of patients were found to carry (likely) pathogenic variants and classified as genetically solved cases. Patients in the less-severe group demonstrated a borderline significant difference in disease progress compared to those in the severe group (p = 0.05). While having only limited power according to its sample size, an obvious strength is the precise clinical and genetic data of this well ascertained cohort. As in published data differences in clinical progress were shown between patients with COL4A5 less-severe and severe variants. Therefore, clinical and segregational data are important for variant (re)classification. Genetic testing should be mandatory allowing early diagnosis and therapy of AS.
Subject(s)
Collagen Type IV/genetics , Genetic Association Studies , Nephritis, Hereditary/genetics , Renal Insufficiency/genetics , Adolescent , Child , Child, Preschool , Early Diagnosis , Female , Genes, X-Linked/genetics , Genetic Testing , Humans , Infant , Kidney/pathology , Male , Nephritis, Hereditary/diagnosis , Nephritis, Hereditary/pathology , Nephritis, Hereditary/therapy , Renal Insufficiency/diagnosis , Renal Insufficiency/pathology , Renal Insufficiency/therapyABSTRACT
BACKGROUND: Secondary hyperparathyroidism (SHPT) may persist after renal transplantation (RTx), inducing hypophosphatemia and hypercalcemia that precludes the use of vitamin D analogs. The calcimimetic cinacalcet improved plasma calcium and parathyroid hormone (PTH) levels in randomized controlled trials in adults after RTx, but pediatric data are scarce. METHODS: In this retrospective study, we analyzed 20 pediatric patients from the Cooperative European Paediatric Renal TransplAnt Initiative (CERTAIN) Registry who received cinacalcet after RTx. The results are presented as median and interquartile range (25th-75th percentile). RESULTS: At 13.7 (11.0-16.5) years of age, 20 pediatric patients received a renal allograft. Cinacalcet was introduced at 0.4 (0.3-2.7) years post-transplant at an estimated glomerular filtration rate (eGFR) of 50 (34-66) mL/min/1.73 m2, plasma calcium of 2.58 (2.39-2.71) mmol/L, age-standardized (z score) phosphate of - 1.7 (- 2.7-- 0.4), and PTH of 136 (95-236) ng/L. The starting dose of cinacalcet was 0.5 (0.3-0.8) mg/kg per day, with a maximum dose of 1.1 (0.5-1.3) mg/kg per day. With a follow-up of 3.0 (1.5-3.6) years on cinacalcet therapy, eGFR remained stable; PTH levels decreased to 66 (56-124) ng/L at the last follow-up (p = 0.015). One patient displayed hypocalcemia (1.8 mmol/L). Cinacalcet was withdrawn in three patients (hypocalcemia, parathyroidectomy, incompliance). Nephrocalcinosis of the graft was not reported. CONCLUSIONS: This pilot study suggests that cinacalcet as off-label therapy for SHPT after pediatric RTx is efficacious in controlling post-transplant SHPT with acceptable tolerability. Continuing cinacalcet even with normal PTH can lead to dangerous life-threatening hypocalcemia. Therefore, at each subsequent visit, the need to continue cinacalcet must be assessed.
Subject(s)
Calcimimetic Agents/administration & dosage , Cinacalcet/administration & dosage , Hyperparathyroidism, Secondary/drug therapy , Hyperparathyroidism, Secondary/etiology , Kidney Failure, Chronic/complications , Adolescent , Calcimimetic Agents/adverse effects , Child , Cinacalcet/adverse effects , Dose-Response Relationship, Drug , Female , Humans , Kidney Failure, Chronic/surgery , Kidney Transplantation , Male , Off-Label Use , Pilot Projects , Registries , Retrospective Studies , Transplant RecipientsABSTRACT
Children with Alport syndrome develop renal failure early in life. Since the safety and efficacy of preemptive nephroprotective therapy are uncertain we conducted a randomized, placebo-controlled, double-blind trial in 14 German sites of pediatric patients with ramipril for three to six years plus six months follow-up to determine these parameters. Pretreated children and those whose parents refused randomization became an open-arm control, which were compared to prospective real-world data from untreated children. The co-primary endpoints were safety (adverse drug reactions) and efficacy (time to progression). Out of 66 oligosymptomatic children, 22 were randomized and 44 joined the open-arm comparison. Ramipril therapy showed no safety issues (total of 216.4 patient-years on ramipril; adverse event rate-ratio 1.00; 95% confidence interval 0.66-1.53). Although not significant, our results cautiously showed that ramipril therapy was effective: in the randomized arm, Ramipril decreased the risk of disease progression by almost half (hazard ratio 0.51 (0.12-2.20)), diminished the slope of albuminuria progression and the decline in glomerular filtration. In adjusted analysis, indications of efficacy were supported by prospective data from participants treated open label compared with untreated children, in whom ramipril again seemed to reduce progression by almost half (0.53 (0.22-1.29)). Incorporating these results into the randomized data by Bayesian evidence synthesis resulted in a more precise estimate of the hazard-ratio of 0.52 (0.19-1.39). Thus, our study shows the safety of early initiation of therapy and supports the hope to slow renal failure by many years, emphasizing the value of preemptive therapy. Hence, screening programs for glomerular hematuria in children and young adults could benefit from inclusion of genetic testing for Alport-related gene-variants.
Subject(s)
Nephritis, Hereditary , Ramipril , Angiotensin-Converting Enzyme Inhibitors/adverse effects , Bayes Theorem , Child , Double-Blind Method , Glomerular Filtration Rate , Humans , Nephritis, Hereditary/genetics , Prospective Studies , Ramipril/adverse effectsABSTRACT
Using real-time SPECT/US fusion imaging, the localization of an uptake defect in DMSA scan could be identified unambiguously after being uncertain in ultrasound alone. Thereby, a localized functional loss, due to history of pyelonephritis, without scarring, but reduced cortical thickness could be verified. DMSA-SPECT/US primarily demonstrates its utility in depiction of renal pathologies and may be a descriptive tool in equivocal constellation of findings.
Subject(s)
Multimodal Imaging , Pyelonephritis/diagnostic imaging , Technetium Tc 99m Dimercaptosuccinic Acid , Tomography, Emission-Computed, Single-Photon , Biological Transport , Child, Preschool , Female , Humans , Infant , Male , Pyelonephritis/metabolism , Pyelonephritis/physiopathology , Technetium Tc 99m Dimercaptosuccinic Acid/metabolism , Time Factors , Ultrasonography , UncertaintyABSTRACT
BACKGROUND: Hypomagnesaemia is present in 40-50% of children with autosomal dominant renal cysts and diabetes syndrome (RCAD). On the contrary, the prevalence of hypomagnesaemia in children with autosomal dominant polycystic kidney disease (ADPKD) has never been examined. We aimed to investigate whether hypomagnesaemia is present in children with polycystic kidney diseases. METHODS: Children with cystic kidney diseases were investigated in a cross-sectional study. Serum concentrations of magnesium (S-Mg) and fractional excretion of magnesium (FE-Mg) were tested. Fifty-four children with ADPKD ( n = 26), autosomal recessive polycystic kidney disease (ARPKD) ( n = 16) and RCAD ( n = 12) with median age of 11.2 (0.6-18.6) years were investigated. RESULTS: Hypomagnesaemia (S-Mg < 0.7 mmol/L) was detected in none of the children with ADPKD/ARPKD and in eight children (67%) with RCAD. Median S-Mg in children with ADPKD/ARPKD was significantly higher than in children with RCAD (0.89 vs. 0.65 mmol/L, P < 0.01). The FE-Mg was increased in 23% of patients with ADPKD/ARPKD (all had chronic kidney disease stages 2-4) and in 63% of patients with RCAD, where it significantly correlated with estimated glomerular filtration rate (r = -0.87, P < 0.01). CONCLUSIONS: Hypomagnesaemia is absent in children with ADPKD or ARPKD and could serve as a marker for differential diagnostics between ADPKD, ARPKD and RCAD in children with cystic kidney diseases of unknown origin where molecular genetic testing is lacking. However, while hypomagnesaemia, in the absence of diuretics, appears to rule out ADPKD and ARPKD, normomagnesaemia does not rule out RCAD at least in those aged <3 years.
Subject(s)
Hypercalciuria/epidemiology , Magnesium/blood , Nephrocalcinosis/epidemiology , Polycystic Kidney, Autosomal Dominant/epidemiology , Renal Tubular Transport, Inborn Errors/epidemiology , Adolescent , Central Nervous System Diseases/blood , Central Nervous System Diseases/diagnosis , Central Nervous System Diseases/epidemiology , Child , Child, Preschool , Cross-Sectional Studies , Dental Enamel/abnormalities , Diabetes Mellitus, Type 2/blood , Diabetes Mellitus, Type 2/diagnosis , Diabetes Mellitus, Type 2/epidemiology , Diagnosis, Differential , Female , Humans , Hypercalciuria/blood , Hypercalciuria/diagnosis , Infant , Infant, Newborn , Kidney Diseases, Cystic/blood , Kidney Diseases, Cystic/diagnosis , Kidney Diseases, Cystic/epidemiology , Male , Nephrocalcinosis/blood , Nephrocalcinosis/diagnosis , Polycystic Kidney, Autosomal Dominant/blood , Polycystic Kidney, Autosomal Dominant/diagnosis , Polycystic Kidney, Autosomal Recessive/blood , Polycystic Kidney, Autosomal Recessive/diagnosis , Polycystic Kidney, Autosomal Recessive/epidemiology , Prevalence , Renal Tubular Transport, Inborn Errors/blood , Renal Tubular Transport, Inborn Errors/diagnosisABSTRACT
BACKGROUND: Variable effects of steroid minimization strategies on blood pressure in pediatric renal transplant recipients have been reported, but data on the effect of steroid withdrawal on ambulatory blood pressure and circadian blood pressure rhythm have not been published so far. METHODS: In a prospective, randomized, multicenter study on steroid withdrawal in pediatric renal transplant recipients (n = 42) on cyclosporine, mycophenolate mofetil, and methylprednisolone, we performed a substudy in 28 patients, aged 11.2 ± 3.8 years, for whom ambulatory blood pressure monitoring (ABPM) data were available. RESULTS: In the steroid-withdrawal group, the percentage of patients with arterial hypertension, defined as systolic and/or diastolic blood pressure values recorded by ABPM > 1.64 SDS and/or antihypertensive medication, at month 15 was significantly lower (35.7%, p = 0.002) than in controls (92.9%). The need of antihypertensive medication dropped significantly by 61.2% (p < 0.000 vs. control), while in controls, it even rose by 69.3%. One year after steroid withdrawal, no patient exhibited hypertensive blood pressure values above the 95th percentile, compared to 35.7% at baseline (p = 0.014) and to 14.3% of control (p = 0.142). The beneficial impact of steroid withdrawal was especially pronounced for nocturnal blood pressure, leading to a recovered circadian rhythm in 71.4% of patients vs. 14.3% at baseline (p = 0.002), while the percentage of controls with an abnormal circadian rhythm (35.7%) did not change. CONCLUSIONS: Steroid withdrawal in pediatric renal transplant recipients with well-preserved allograft function is associated with less arterial hypertension recorded by ABPM and recovery of circadian blood pressure rhythm by restoration of nocturnal blood pressure dipping.
Subject(s)
Glucocorticoids/adverse effects , Hypertension/prevention & control , Immunosuppressive Agents/adverse effects , Kidney Transplantation/adverse effects , Withholding Treatment , Adolescent , Allografts/immunology , Allografts/physiopathology , Blood Pressure/drug effects , Blood Pressure Monitoring, Ambulatory , Child , Circadian Rhythm/physiology , Cyclosporine/administration & dosage , Cyclosporine/adverse effects , Female , Glomerular Filtration Rate/physiology , Glucocorticoids/administration & dosage , Graft Rejection/immunology , Graft Rejection/physiopathology , Graft Rejection/prevention & control , Humans , Hypertension/chemically induced , Hypertension/diagnosis , Immunosuppressive Agents/administration & dosage , Kidney/immunology , Kidney/physiopathology , Male , Methylprednisolone , Mycophenolic Acid/administration & dosage , Mycophenolic Acid/adverse effects , Prospective Studies , Transplantation, Homologous/adverse effectsABSTRACT
BACKGROUND: Proteinuria is a common complication in adults with autosomal dominant polycystic kidney disease (ADPKD) and serves as a risk factor for progression. However, proteinuria has rarely been examined in children with ADPKD and the type of proteinuria has not yet been investigated. The aim of the study was to assess the prevalence and to analyse the types of proteinuria in children with ADPKD. METHODS: Children with ADPKD followed-up in our tertiary centres during the years 2012-2013 were investigated in a cross-sectional study. Morning urine was tested for total protein (PROT), albumin (ALB) and alpha-1-microglobulin (AMG). Renal function was assessed from serum creatinine as estimated glomerular filtration rate. RESULTS: Thirty-seven children of median age 11.2 (2.0-18.0) years were investigated. Median (range) PROT, ALB and AMG (in mg/mmol creatinine) were 15.1 (6.2-64.8), 2.54 (0.54-37.25) and 3.22 (0.04-10.16), respectively. Pathological total proteinuria (>22) was found in 30% of children, albuminuria (>2.2) in 49% of children and alpha-1-microglobulinuria (>0.55) in 65% of children. No correlation was found between PROT, ALB or AMG and office blood pressure, kidney size or estimated glomerular filtration rate. CONCLUSIONS: Proteinuria in children with ADPKD is a frequent finding, the most common type is tubular proteinuria. It should be measured in all ADPKD children.
Subject(s)
Albuminuria/epidemiology , Alpha-Globulins/urine , Polycystic Kidney, Autosomal Dominant/physiopathology , Proteinuria/epidemiology , Adolescent , Albuminuria/etiology , Blood Pressure , Child , Child, Preschool , Creatinine/blood , Cross-Sectional Studies , Disease Progression , Female , Glomerular Filtration Rate , Humans , Male , Prevalence , Proteinuria/etiology , Risk FactorsABSTRACT
OBJECTIVE: The purpose of this study is to compare functional MR urography (MRU) with the results of ultrasound and radionuclide 99mTc-mercaptoacetyltriglycine (MAG3) scintigraphy in evaluating morphologic findings, split renal function, and urinary tract obstruction in pediatric patients. MATERIALS AND METHODS: Pediatric patients with proven congenital anomalies of the kidney and urinary tract were included (n = 112). The morphologic findings of MRU were compared with previous diagnostic ultrasound findings. For evaluation of split renal function and urinary tract obstruction, MAG3 scintigraphy was used as a reference standard. RESULTS: MRU provided detailed morphologic information of the whole urinary tract for all 112 patients. In 94.6% of cases (n = 106), diagnostic findings could be verified, and in 5.4% of cases (n = 6), more detailed information could be gained. Equivalent split renal function showed good concordance between functional MRU and MAG3 scintigraphy. However, in kidneys with restricted function (< 35%), functional MRU underestimated the remaining renal function, with a mean difference of 6.6% and an SD of 24.4%. For evaluation of relevant urinary tract obstruction, the sensitivity of functional MRU was 100%, specificity was 81.6%, positive predictive value was 70.8%, and negative predictive value was 100%. CONCLUSION: Regarding split renal function, functional MRU shows a lack of accuracy in comparison with the clinical standard MAG3 scintigraphy, especially in patients with severely diminished function of one kidney. However, functional MRU allows an adequate assessment of urinary tract obstruction and a high-resolution morphologic evaluation of the whole urinary tract. Thus, functional MRU is suitable to add diagnostic value, especially as a complementary examination for complex individual cases in the presurgical state.
Subject(s)
Magnetic Resonance Imaging/methods , Radionuclide Imaging/methods , Ultrasonography/methods , Urogenital Abnormalities/diagnostic imaging , Adolescent , Child , Child, Preschool , Diagnosis, Differential , Female , Humans , Infant , Infant, Newborn , Kidney Function Tests , Male , Organotechnetium Compounds , Radiopharmaceuticals , Retrospective Studies , Tiopronin , Urogenital Abnormalities/physiopathologyABSTRACT
OBJECTIVES: It is known that transition, as a shift of care, marks a vulnerable phase in the adolescents' lives with an increased risk for non-adherence and allograft failure. Still, the transition process of adolescents and young adults living with a kidney transplant in Germany is not well defined. The present research aims to assess transition-relevant structures for this group of young people. Special attention is paid to the timing of the process. SETTING: In an observational study, we visited 21 departments of paediatric nephrology in Germany. Participants were doctors (n=19), nurses (n=14) and psychosocial staff (n=16) who were responsible for transition in the relevant centres. Structural elements were surveyed using a short questionnaire. The experiential viewpoint was collected by interviews which were transcribedverbatim before thematic analysis was performed. RESULTS: This study highlights that professionals working within paediatric nephrology in Germany are well aware of the importance of successful transition. Key elements of transitional care are well understood and mutually agreed on. Nonetheless, implementation within daily routine seems challenging, and the absence of written, structured procedures may hamper successful transition. CONCLUSIONS: While professionals aim for an individual timing of transfer based on medical, social, emotional and structural aspects, rigid regulations on transfer age as given by the relevant health authorities add on to the challenge. TRIAL REGISTRATION NUMBER: ISRCTN Registry no 22988897; results (phase I) and pre-results (phase II).
Subject(s)
Kidney Transplantation/psychology , Transition to Adult Care/organization & administration , Transition to Adult Care/standards , Adolescent , Age Factors , Female , Germany , Humans , Interviews as Topic , Male , Qualitative Research , Surveys and Questionnaires , Time Factors , Young AdultABSTRACT
BACKGROUND: Febrile urinary tract infections (fUTIs) are common after kidney transplantation (KTx); however, prospective data in a multicenter pediatric cohort are lacking. We designed a prospective registry to record data on fUTI before and after pediatric KTx. METHODS: Ninety-eight children (58 boys and 40 girls) ≤ 18 years from 14 mid-European centers received a kidney transplant and completed a 2-year follow-up. RESULTS: Posttransplant, 38.7% of patients had at least one fUTI compared with 21.4% before KTx (p = 0.002). Before KTx, fUTI was more frequent in patients with congenital anomalies of kidneys and urinary tract (CAKUT) vs. patients without (38% vs. 12%; p = 0.005). After KTx, fUTI were equally frequent in both groups (48.7% vs. 32.2%; p = 0.14). First fUTI posttransplant occurred earlier in boys compared with girls: median range 4 vs. 13.5 years (p = 0.002). Graft function worsened (p < 0.001) during fUTI, but no difference was recorded after 2 years. At least one recurrence of fUTI was encountered in 58%. CONCLUSION: This prospective study confirms a high incidence of fUTI after pediatric KTx, which is not restricted to patients with CAKUT; fUTIs have a negative impact on graft function during the infectious episode but not on 2-year graft outcome.
Subject(s)
Delayed Graft Function/etiology , Fever/epidemiology , Kidney Diseases/surgery , Kidney Transplantation/adverse effects , Urinary Tract Infections/epidemiology , Adolescent , Child , Child, Preschool , Delayed Graft Function/epidemiology , Europe/epidemiology , Female , Fever/etiology , Graft Rejection/epidemiology , Graft Rejection/etiology , Humans , Incidence , Male , Prospective Studies , Risk Factors , Sex Factors , Treatment Outcome , Urinary Tract Infections/complications , Urinary Tract Infections/etiologyABSTRACT
Transition from child to adult-oriented care is widely regarded a challenging period for young people with kidney transplants and is associated with a high risk of graft failure. We analyzed the existing transition structures in Germany and Austria using a questionnaire and retrospective data of 119 patients transferred in 2011 to 2012. Most centers (73%) confirmed agreements on the transition procedure. Patients' age at transfer was subject to regulation in 73% (18 years). Median age at transition was 18.3 years (16.5-36.7). Median serum creatinine increased from 123 to 132 µmol/L over the 12 month observation period before transfer (P = 0.002). A total of 25/119 patients showed increased creatinine ≥ 20% just before transfer. Biopsy proven rejection was found in 10/119 patients. Three patients lost their graft due to chronic graft nephropathy.Mean coefficient of variation (CoV%) of immunosuppression levels was 0.20 ± 0.1. Increased creatinine levels ≥ 20% just before transfer were less frequently seen in patients with CoVâ< 0.20 (P = 0.007). The majority of pediatric nephrology centers have internal agreements on transitional care. More than half of the patients had CoV of immunosuppression trough levels consistent with good adherence. Although, 20% of the patients showed increase in serum creatinine close to transfer.
Subject(s)
Kidney Transplantation/statistics & numerical data , Transition to Adult Care/organization & administration , Transition to Adult Care/statistics & numerical data , Adolescent , Adult , Austria , Female , Germany , Graft Rejection/epidemiology , Humans , Immunosuppressive Agents/therapeutic use , Male , Medication Adherence/statistics & numerical data , Retrospective Studies , Young AdultABSTRACT
BACKGROUND: Growth restriction and retarded bone age are common findings in children with chronic kidney disease (CKD). We compared the automated BoneXpert™ method with the manual assessment of an X-ray of the non-dominant hand. METHODS: In this retrospective multicenter study, 359 patients with CKD stages 2-5, aged 2-14.5 (girls) or 2.5-17 years (boys) were included. Bone age was determined manually by three experts (according to Greulich and Pyle). Automated determination of bone age was performed using the image analysis software BoneXpert™. RESULTS: There was a strong correlation between the automatic and the manual method (r = 0.983, p < 0.001). The automatic method tended to generate higher bone age values (0.64 ± 0.73 years) in the younger patients (4-5 years) and to underestimate retardation or acceleration of bone age. The so-called "bone health index" (BHI) was reduced in comparison to the reference population. Bone health index standard deviation score (BHI-SDS) was not related to the stage of CKD, but weakly negatively correlated with plasma PTH concentrations (r = 0.12, p = 0.019). CONCLUSIONS: BoneXpert™ allows an objective, time-saving, and in general valid bone age assessment in children with CKD. Possible underestimation of retarded or accelerated bone age should be taken into account. Validation of the BHI needs further study.
Subject(s)
Age Determination by Skeleton/methods , Bone Diseases/diagnosis , Bone Diseases/etiology , Renal Insufficiency, Chronic/complications , Adolescent , Automation , Bone Diseases/diagnostic imaging , Child , Child, Preschool , Female , Growth Disorders/etiology , Growth Disorders/pathology , Hand/diagnostic imaging , Health Status , Humans , Image Processing, Computer-Assisted , Kidney Function Tests , Male , Parathyroid Hormone/blood , Renal Insufficiency, Chronic/diagnostic imaging , Reproducibility of Results , Retrospective StudiesABSTRACT
OBJECTIVE: Functional urinary incontinence causes considerable morbidity in 8.4% of school-age children, mainly girls. To compare oxybutynin, placebo, and bladder training in overactive bladder (OAB), and cognitive treatment and pelvic floor training in dysfunctional voiding (DV), a multi-center controlled trial was designed, the European Bladder Dysfunction Study. METHODS: Seventy girls and 27 boys with clinically diagnosed OAB and urge incontinence were randomly allocated to placebo, oxybutynin, or bladder training (branch I), and 89 girls and 16 boys with clinically diagnosed DV to either cognitive treatment or pelvic floor training (branch II). All children received standardized cognitive treatment, to which these interventions were added. The main outcome variable was daytime incontinence with/without urinary tract infections. Urodynamic studies were performed before and after treatment. RESULTS: In branch I, the 15% full response evolved to cure rates of 39% for placebo, 43% for oxybutynin, and 44% for bladder training. In branch II, the 25% full response evolved to cure rates of 52% for controls and 49% for pelvic floor training. Before treatment, detrusor overactivity (OAB) or pelvic floor overactivity (DV) did not correlate with the clinical diagnosis. After treatment these urodynamic patterns occurred de novo in at least 20%. CONCLUSION: The mismatch between urodynamic patterns and clinical symptoms explains why cognitive treatment was the key to success, not the added interventions. Unpredictable changes in urodynamic patterns over time, the response to cognitive treatment, and the gender-specific prevalence suggest social stress might be a cause for the symptoms, mediated by corticotropin-releasing factor signaling pathways.
Subject(s)
Cognitive Behavioral Therapy/methods , Mandelic Acids/therapeutic use , Physical Therapy Modalities , Urinary Bladder, Overactive/therapy , Urinary Incontinence, Urge/therapy , Urination Disorders/therapy , Urological Agents/therapeutic use , Child , Combined Modality Therapy , Female , Humans , Male , Pelvic Floor/physiopathology , Treatment Outcome , Urinary Bladder/physiopathology , Urinary Bladder, Overactive/complications , Urinary Bladder, Overactive/physiopathology , Urinary Incontinence, Urge/complications , Urinary Incontinence, Urge/physiopathology , Urination Disorders/physiopathology , Urodynamics/physiologyABSTRACT
BACKGROUND: The hereditary kidney disease Alport syndrome (AS) has become a treatable disease: intervention with angiotensin-converting enzyme (ACE)-inhibitors delays end stage renal failure by years. The efficiency of ACE inhibition depends on the onset of therapy-the earlier the better. Therefore, early diagnosis has become increasingly important. To date, robust diagnosis requires renal biopsy and/or expensive genetic analysis, which is mostly performed late after onset of the profound clinical symptoms of this progressive renal disease. Thus, disease biomarkers enabling low-invasive screening are urgently required. METHODS: Fourteen potential proteomic candidate markers (proteins) identified in a previous study in sera from patients exhibiting manifest AS were evaluated in the plasma, serum, and urine collected from a cohort of 132 subjects, including patients with AS and other nephropathies and healthy controls. Quantitation was performed by immunoassays. RESULTS: The serum and plasma levels of none of the 14 proteins evaluated were significantly different among the three groups and therefore could not be used to discriminate between the groups. In contrast, the levels of various biomarker combinations in the urine were significantly different between AS patients and healthy controls. Importantly, some combinations had the potential to discriminate between AS and other nephropathies. CONCLUSIONS: These findings open a window of opportunity for the sensitive and specific early diagnosis of AS. Our results increase the potential for larger scale evaluation of an increased number of patients.
Subject(s)
Biomarkers/analysis , Nephritis, Hereditary/diagnosis , Proteomics/methods , ADAM Proteins/analysis , Fibronectins/analysis , Humans , Immunoassay , Matrix Metalloproteinase 2/analysis , Matrix Metalloproteinase 9/analysis , Membrane Proteins/analysis , Myosins/analysisABSTRACT
BACKGROUND: The epidemiology and morbidity of Epstein-Barr virus (EBV) infection in pediatric renal transplant recipients have been characterized insufficiently. METHODS: In a prospective, multicenter study among 106 pediatric kidney allograft recipients aged 11.4 ± 5.9 years, we investigated the epidemiology of EBV infection and the relationship between EBV load, EBV serology, and EBV-related morbidity (posttransplant lymphoproliferative disease [PTLD] or symptomatic EBV infection, defined as flu-like symptoms or infectious mononucleosis). RESULTS: EBV primary infection occurred in 27 of 43 (63%) seronegative patients and reactivation/reinfection in 28 of 63 (44%) seropositive patients. There was no association between the degree or duration of EBV load and EBV-related morbidity: The vast majority (17 of 18 [94%]) of patients with a high, persistent EBV load remained PTLD-free throughout a follow-up of 5.0 ± 1.3 years, while 2 of 3 (66%) patients with EBV-related PTLD exhibited only a low EBV load beforehand. Eight of 18 (44%) patients with a high, persistent EBV load remained asymptomatic during a follow-up of 5.3 ± 2.9 years. Multivariate analysis identified the EBV high-risk (D(+)/R(-)) serostatus (odds ratio [OR], 7.07; P < .05), the presence of human leukocyte antigen (HLA)-DR7 (OR, 5.65; P < .05), and the intensity of the immunosuppressive therapy (OR, 1.53; P < .01) as independent risk factors for the development of a symptomatic EBV infection. CONCLUSIONS: Presence of EBV high-risk seroconstellation, HLA-DR7, and intensity of immunosuppressive therapy are significant risk factors for a symptomatic EBV infection, whereas there is no close association between the degree or duration of EBV load and EBV-related morbidity. Clinical Trials Registration. NCT00963248.
Subject(s)
Epstein-Barr Virus Infections/epidemiology , Herpesvirus 4, Human/isolation & purification , Kidney Transplantation/statistics & numerical data , Adolescent , Analysis of Variance , Antiviral Agents/therapeutic use , Child , Epstein-Barr Virus Infections/immunology , Epstein-Barr Virus Infections/therapy , Epstein-Barr Virus Infections/virology , Female , Graft Rejection/drug therapy , Graft Rejection/prevention & control , Herpesvirus 4, Human/immunology , Humans , Immunosuppressive Agents/therapeutic use , Lymphoproliferative Disorders/epidemiology , Lymphoproliferative Disorders/etiology , Lymphoproliferative Disorders/virology , Male , Morbidity , Prospective Studies , Statistics, Nonparametric , Transplants/statistics & numerical data , Viral LoadABSTRACT
BACKGROUND AND OBJECTIVES: Double-chamber peritoneal dialysis fluids exert less toxicity by their neutral pH and reduced glucose degradation product content. The role of the buffer compound (lactate and bicarbonate) has not been defined in humans. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: A multicenter randomized controlled trial in 37 children on automated peritoneal dialysis was performed. After a 2-month run-in period with conventional peritoneal dialysis fluids, patients were randomized to neutral-pH, low-glucose degradation product peritoneal dialysis fluids with 35 mM lactate or 34 mM bicarbonate content. Clinical and biochemical monitoring was performed monthly, and peritoneal equilibration tests and 24-hour clearance studies were performed at 0, 3, 6, and 10 months. RESULTS: No statistically significant difference in capillary blood pH, serum bicarbonate, or oral buffer supplementation emerged during the study. At baseline, peritoneal solute equilibration and clearance rates were similar. During the study, 4-hour dialysis to plasma ratio of creatinine tended to increase, and 24-hour dialytic creatinine and phosphate clearance increased with lactate peritoneal dialysis fluid but not with bicarbonate peritoneal dialysis fluid. Daily net ultrafiltration, which was similar at baseline (lactate fluid=5.4±2.6 ml/g glucose exposure, bicarbonate fluid=4.9±1.9 ml/g glucose exposure), decreased to 4.6±1.0 ml/g glucose exposure in the lactate peritoneal dialysis fluid group, whereas it increased to 5.1±1.7 ml/g glucose exposure in the bicarbonate content peritoneal dialysis fluid group (P=0.006 for interaction). CONCLUSIONS: When using biocompatible peritoneal dialysis fluids, equally good acidosis control is achieved with lactate and bicarbonate buffers. Improved long-term preservation of peritoneal membrane function may, however, be achieved with bicarbonate-based peritoneal dialysis fluids.
Subject(s)
Dialysis Solutions/administration & dosage , Kidney Failure, Chronic/therapy , Peritoneal Dialysis/methods , Acid-Base Equilibrium , Acidosis/metabolism , Acidosis/prevention & control , Adolescent , Bicarbonates/administration & dosage , Body Water/metabolism , Buffers , Child , Child, Preschool , Dialysis Solutions/adverse effects , Female , Humans , Infant , Infant, Newborn , Kidney Failure, Chronic/metabolism , Lactic Acid/administration & dosage , Male , Peritoneal Dialysis/adverse effects , Peritoneum/metabolism , Treatment Outcome , Young AdultABSTRACT
Epstein-Barr virus (EBV) primary infection constitutes a serious risk for pediatric transplant recipients, particularly as regards the development of EBV-related post-transplant lymphoproliferative disease (PTLD). Currently, there is no established prophylactic regimen. We investigated the association between chemoprophylaxis with valganciclovir (VGCV) or ganciclovir (GCV) and the incidence of EBV viremia in EBV-naïve pediatric renal transplant recipients (R-) who had received a graft from an EBV-positive donor (D+) and are therefore at high risk of EBV primary infection. In a prospective, multicenter trial (n = 114), we compared a cohort on chemoprophylaxis (n = 20) with a similar control cohort without chemoprophylaxis (n = 8). Over the 1-year study period, antiviral prophylaxis with VGCV/GCV was associated with a significantly decreased incidence of EBV primary infection: 9/20 patients (45%) in the prophylaxis group experienced an EBV primary infection compared to 8/8 controls (100%) (P < 0.0001). Chemoprophylaxis was associated with a significantly lower EBV viral load (P < 0.001). Type or intensity of immunosuppressive therapy did not influence the occurrence of EBV primary infection or the level/persistence of EBV viral load. Chemoprophylaxis with VGCV/GCV is associated with a reduced incidence of EBV viremia in high-risk pediatric kidney allograft recipients in the first year post-transplant. (ClinicalTrials.gov number: NCT00963248).
Subject(s)
Epstein-Barr Virus Infections/prevention & control , Ganciclovir/analogs & derivatives , Ganciclovir/therapeutic use , Herpesvirus 4, Human/metabolism , Kidney Transplantation/methods , Adult , Antiviral Agents/therapeutic use , Chemoprevention/methods , Child , Cohort Studies , Female , Humans , Immunosuppressive Agents/therapeutic use , Lymphoproliferative Disorders/etiology , Male , Pediatrics/methods , Prospective Studies , ValganciclovirABSTRACT
Microalbuminuria serves as an early marker of hypertension-related renal damage in adults. However, data on the prevalence of microalbuminuria in paediatric hypertensive patients in general and in children with white-coat hypertension (WCH) specifically are lacking. The aim of our study was to investigate the prevalence of microalbuminuria in children with primary hypertension (PH) and WCH, respectively. This was a retrospective case review of children with PH and WCH treated at three paediatric nephrology centres. Untreated children with either form of hypertension for whom measurements of urinary albumin excretion (UAE) had been performed were enrolled in the study. The study cohort comprised 52 children (39 boys) with hypertension (26 children with PH, 26 with WCH). Microalbuminuria (>3.2 mg/mmol creatinine) was present in 20% of children with PH and none of the children with WCH (p < 0.01). Children with PH had a higher median UAE than those with WCH (1.27 ± 1.92 vs. 0.66 ± 0.46 mg/mmol creatinine, p < 0.05). Based on these results, we suggest that children with PH have an increased prevalence of microalbuminuria, while children with WCH show no signs of hypertension-related renal damage.
Subject(s)
Albuminuria/etiology , White Coat Hypertension/complications , Adolescent , Blood Pressure Monitoring, Ambulatory , Child , Creatinine/metabolism , Female , Humans , Male , White Coat Hypertension/physiopathologyABSTRACT
Posterior urethral valves (PUV) associated with renal dysplasia are one of the most common causes of end stage kidney disease (ESKD) in childhood. In order to identify risk factors for the progression of this condition to early renal failure, we have retrospectively analyzed the clinical course, renal function, and first postnatal renal ultrasound in a sample of 42 young male patients with PUV, who were followed at a single center. Twelve (28.6%) were diagnosed with ESKD at a median age of 11.3 years. Our comparison of PUV patients without decreased estimated glomerular filtration rate (eGFR) (group A; K/DOQI CKD stage 0-1) with PUV patients showing a decreased eGFR (group B; K/DOQI CKD stage 2-5) revealed the following significant risk factors for loss of eGFR: renal volume <3rd percentile (P < 0.001), elevated echogenicity (P = 0.001), pathologic corticomedullary differentiation (P < 0.001), >3 febrile urinary tract infections (P = 0.012), and decreased eGFR at 1 year of age (P < 0.001). Receiver operating characteristic curve analysis in the cohort confirms that patients showing a renal volume >88.2 ml/m(2) body surface area (BSA) are not at risk to develop K/DOQI CKD stage 5 (sensitivity 75%, specificity 77.3%, positive/negative predictive value 37.5/94.4%). Ultrasound promises to be a valuable tool for identifying endangered patients.
Subject(s)
Renal Insufficiency/etiology , Urethra/abnormalities , Body Surface Area , Child , Child, Preschool , Glomerular Filtration Rate , Humans , Infant , Infant, Newborn , Kidney/diagnostic imaging , Male , ROC Curve , Risk Factors , Ultrasonography , Urinary Tract Infections/epidemiologyABSTRACT
We report a male newborn presenting with sonographically normal kidneys, oligohydramnios during late pregnancy, and persisting anuric renal failure. Despite intensive treatment, the patient suffered from severe hypotension and died at the age of 4 weeks. At autopsy, kidneys were found to be normal; on histology, deranged renal structures, in particular proximal tubuli and vessels, were noted, leading to the diagnosis of renal tubular dysgenesis (RTD). The diagnosis was confirmed by 2 heterozygous nonsense mutations of the ACE gene. Because the recurrence rate of RTD is 25% for the autosomal recessive trait, knowledge and genetic diagnosis of the disease is important for the parents.