All-solid-state K+ sensing array based on Au@polystyrene nanocomposites.

All-solid-state ion selective electrodes (ASS-ISEs) are easy to miniaturize and array, meeting the needs of home sensing devices. However, ASS-ISEs still faces challenges in accuracy and stability due to basic potential changes caused by non-specific adsorption of charged background compositions and the complex electrode preparation steps. To this end, our group successfully subtracted the background signal by integrating a self-calibrating channel in the sensing array and simplified the electrode preparation steps by preparing multi-functional PS-Au nanocomposites. However, the uniformity and gold content of PS-Au nanocomposites are difficult to control, so Au@PS nanocomposites are prepared as sensor materials in this paper to further reduce the differences between batches of electrodes. K+ Au@PS sensing array can be obtained by directly dropping Au@PS nanocomposites on the screen-printed carbon electrodes (SPCEs), which shows a near Nernstian behavior in the range 1.0 × 10-3 M to 0.3 M and good reproducibility in real sample testing. The detection results by K+ Au@PS sensing array for K+ in human morning urine agreed well with that tested by ICP-AES, which make the K+-ASS-ISE suitable for home health monitoring.

The impact of simultaneous inoculation with Torulaspora delbrueckii and Hanseniaspora uvarum combined with Saccharomyces cerevisiae on chemical and sensory quality of Sauvignon blanc wines.

Non-Saccharomyces yeasts have great potential in improving wine quality, showing personality characteristics, and highlighting the terroir of wine. In this study, we evaluated the impact of simultaneous inoculation with the non-Saccharomyces yeasts Torulaspora delbrueckii or (and) Hanseniaspora uvarum in combination with Saccharomyces cerevisiae (EC1118 or VL3) on the aromatic compounds and sensory quality of Sauvignon blanc wines. The growth of yeast groups in the alcoholic fermentation process was tracked using fluorescence in situ hybridization. The presence of non-Saccharomyces yeast notably impacted the distribution of S. cerevisiae and was related to the species of yeast. The co-fermentation of H. uvarum and S. cerevisiae improved the content of total esters, especially acetate esters. Simultaneous inoculation of T. delbrueckii or (and) H. uvarum significantly increased the content of total terpenes, especially linalool. Similar results were found for some higher alcohols and organic acids. Sensory evaluation showed that the wines mixed fermentation with H. uvarum had significantly tropical fruit aroma characteristics. Citrus and mineral notes, typical aroma characteristics of Sauvignon blanc wine, were enhanced by mixed fermentation strategies with T. delbrueckii or (and) H. uvarum and different S. cerevisiae. Hence, co-fermentation by T. delbrueckii or H. uvarum combined with S. cerevisiae could significantly improve the sensory quality of Sauvignon blanc wine.

Enhanced hypoxanthine utilization for cAMP salvage synthesis efficiently by Arthrobacter sp. CCTCC 2013431 via xanthine oxidase inhibition.

When hypoxanthine was utilized as the activator for the salvage pathway in cAMP synthesis, xanthine oxidase would generate in quantity leading to low hypoxanthine conversion ratios and cell viability. To enhance cAMP salvage synthesis, fermentations with citrate/luteolin and hypoxanthine coupling added were conducted in a 7 L bioreactor and then multiple physiological indicators of fermentation with luteolin addition were assayed. Due to hypoxanthine feeding, cAMP productivity reached 0.066 g/(L·h) with 43.5% higher than control, however, cAMP synthesis, cell growth and glucose uptake all ceased at 50 h which was shortened by 22 h in comparison to control. The addition of citrate resulted in the cessation of fermentation at 61 h, on the contrary, owing to luteolin addition, cAMP fermentation performance was enhanced significantly during the whole fermentation period (72 h) with higher hypoxanthine conversion ratios and cAMP contents when compared with citrate and only hypoxanthine added batches. Multiple physiological indicators revealed that luteolin inhibited xanthine oxidase activity reducing hypoxanthine decomposition and ROS generation. ATP/AMP, NADH/NAD+ and NADPH/NADP+ were significantly increased especially at the late phase. Moreover, HPRT, PUP expression contents and corresponding gene transcription levels were also elevated. Luteolin could inhibit xanthine oxidase activity and further decrease hypoxanthine decomposition and ROS generation leading to higher hypoxanthine conversion and less cell damage for cAMP salvage synthesis efficiently.

Factors influencing the development of nursing professionalism: a descriptive qualitative study.

BACKGROUND: The shortage of nurses threatens the entire healthcare system, and nursing professionalism can improve nurse retention and enhance the quality of care. However, nursing professionalism is dynamic, and the factors influencing its development are not fully understood. METHODS: A qualitative descriptive study was conducted. Using maximum variation and purposive sampling, 14 southern and northern China participants were recruited. Semi-structured interviews were conducted from May 2022 to August 2023 in face-to-face conversations in offices in the workplace or via voice calls. The interviews were transcribed verbatim and analyzed via thematic analysis. RESULTS: Three main themes emerged: (1) nourishment factors: promoting early sprouting; (2) growth factors: the power of self-activation and overcoming challenges; and (3) rootedness factors: stability and upward momentum. Participants described the early acquisition of nursing professionalism as derived from personality traits, family upbringing, and school professional education, promoting the growth of nursing professionalism through self-activation and overcoming challenges, and maintaining the stable and upward development of nursing professionalism through an upward atmosphere and external motivation. CONCLUSION: We revealed the dynamic factors that influence the development of nursing professionalism, including "nourishment factors", "growth factors", and "rootedness factors". Our findings provide a foundation for future development of nursing professionalism cultivation strategies. Nursing administrators can guide the development of nurses' professionalism from many angles according to the stage they are in, and the development of professionalism deserves more attention. In the future, we can no longer consider the development of nursing professionalism solely as the responsibility of individual nurses; the power of families, organizations, and society is indispensable to jointly promoting nursing professionalism among nurses.

Quorum sensing: cell-to-cell communication in Saccharomyces cerevisiae.

Quorum sensing (QS) is one of the most well-studied cell-to-cell communication mechanisms in microorganisms. This intercellular communication process in Saccharomyces cerevisiae began to attract more and more attention for researchers since 2006, and phenylethanol, tryptophol, and tyrosol have been proven to be the main quorum sensing molecules (QSMs) of S. cerevisiae. In this paper, the research history and hotspots of QS in S. cerevisiae are reviewed, in particular, the QS system of S. cerevisiae is introduced from the aspects of regulation mechanism of QSMs synthesis, influencing factors of QSMs production, and response mechanism of QSMs. Finally, the employment of QS in adaptation to stress, fermentation products increasing, and food preservation in S. cerevisiae was reviewed. This review will be useful for investigating the microbial interactions of S. cerevisiae, will be helpful for the fermentation process in which yeast participates, and will provide an important reference for future research on S. cerevisiae QS.

Heterogeneity in Adolescents' Non-Suicidal Self-Injury Behaviour Trajectories Based on the Group-Based Trajectory Model and a Decision Tree Analysis of Family-Related Determinants.

Background: Non-suicidal self-injury (NSSI) is a global public health problem. While some studies have noted the importance of behavioural development patterns in NSSI, most NSSI research is based on cross-sectional survey data. Few studies explore the time-series heterogeneity of trajectories of NSSI symptoms and family-related influencing factors of NSSI. Aim: The purpose of the study was to identify the heterogeneity in trajectories of adolescents' NSSI behaviour and their family-related influencing factors. Methods: The group-based trajectory model (GBTM) was used to identify the heterogeneity in the NSSI behaviour trajectories of 208 adolescents in China. Next, the decision tree model (DTM) was used to analyse which family related factors influence the trajectory type. Results: The GBTM revealed two heterogeneous trajectories of NSSI behaviour: the high-risk and low-risk NSSI behaviour groups. Next, DTM's average accuracy was 83.2%. A total of seven independent variables were used for the DTM: gender, number of NSSIs in the past month, and family economic, family structure, family conflict, parental psychological control, parental behavior control and family intimacy risks. Family conflict risk was located at the root node and was the most important factor. Conclusion: Heterogeneity within the population should be considered in the management of adolescents' NSSI behaviours. Further, from the perspective of family system theory and cumulative risk, focusing on the adverse effect of multiple risk factors on adolescents' NSSI addiction is more meaningful rather than the impact of single risk factors. Studies that use time series data should focus on the trend of dynamic changes in NSSI addiction characteristics over time.

Modulation of the Coordination Environment of Copper for Stable CO2 Electroreduction with Tunable Selectivity.

Manipulating the product selectivity of an electrochemical CO2 reduction reaction (CO2RR) is challenging due to the unclear and uncontrollable active sites. Here, we report stable CO2RR operation with tunable product selectivity over a family of molecule-modulated copper catalysts. The coordination environment of Cu in catalysts is modulated by an imidazole-based molecule via different synthetic routes. Various carbonaceous products ranging from carbon monoxide, methane, and ethylene were selectively produced via, respectively, tuning the coordination environment of copper atoms from Cu-N, Cu-C, and Cu-Cu. Density functional theory (DFT) calculations reveal that the Cu-N sites weaken the adsorption energy of the *CO intermediate, which is beneficial for CO desorption. The Cu-C and Cu-Cu sites, respectively, facilitate the formation of *OCOH and *(CO)2 intermediates, favoring the CH4 and C2H4 pathways. This work provides a stable and simple model system for studying the influence of coordination elements on the product selectivity of CO2RR.

Optimization design of the sound absorbing structure of double-layer porous metal material with air layer based on genetic algorithm.

An acoustic absorption structure of a double-layer porous metal material with air layers is proposed. The Johnson-Champoux-Allard (JCA) model combined with the transfer matrix method (TMM) was used to establish the theoretical calculation model of the sound absorption coefficient (SAC). Meanwhile, the SAC between 500 and 6300 Hz were measured with an impedance tube. The errors between the theoretical and experimental values were compared to illustrate the good predictability of the theoretical model within the inverse estimations of the transport properties. The effects of the material placement order, material thickness, and cavity depth on the sound absorption performance from 200 to 5000 Hz were analyzed using the theoretical model. Further, a multi-objective function genetic algorithm was used to optimize the porous material's thickness and SAC to obtain an acoustic structure with a smaller thickness and higher sound absorption. A series of optimal solutions were obtained for acoustic structures with a total thickness of less than 70 mm. When the total thickness of the foam metal was 33.57 mm, the average SAC reached 0.853, which was significantly lower than the total thickness of the previous experiments. The multi-objective function genetic algorithm can provide a reliable solution for the optimal design of most sound-absorbing structures.

Barriers to home exercise for patients with Parkinson's disease: a qualitative study.

OBJECTIVE: This study aimed to explore the barriers to home exercise for patients with Parkinson's disease (PwPDs) and to provide guidelines for healthcare providers to build and implement home exercise strategies for PwPDs. DESIGN: A qualitative descriptive method was used. Semistructured interviews were conducted and thematic analysis was employed. SETTING: The study was conducted at the Department of Neurology at a grade 3 Class A general hospital in China. PARTICIPANTS: A total of 24 participants were interviewed, including 10 PwPDs, 7 caregivers, 4 nurses, 1head nurse, and 2 Parkinson's clinicians. RESULTS: Five themes were identified in this analysis. (1) Psychosomatic stress and low activity; (2) Lack of early rehabilitation authorisation; (3) Poor 'flow' state of home exercise; (4) iInaccessibility of continued service; (5) Sociocultural impact on family coping. CONCLUSION: PwPDs, caregivers and specialised medical staff raised the challenges faced by patients' home exercises from different perspectives. We can improve services and integrate resources through the management of multi-disciplinary, early rehabilitation authorisation, exercise experience, continuous service mode, and family coping strategies under different cultures to gradually adjust the home exercise behaviour of PwPDs.

Selective Electrochemical Hydrogenation of Phenol with Earth-abundant Ni-MoO2 Heterostructured Catalysts: Effect of Oxygen Vacancy on Product Selectivity.

Herein, we report highly efficient carbon supported Ni-MoO2 heterostructured catalysts for the electrochemical hydrogenation (ECH) of phenol in 0.10 M aqueous sulfuric acid (pH 0.7) at 60 °C. Highest yields for cyclohexanol and cyclohexanone of 95 % and 86 % with faradaic efficiencies of ∼50 % are obtained with catalysts bearing high and low densities of oxygen vacancy (Ov ) sites, respectively. In situ diffuse reflectance infrared spectroscopy and density functional theory calculations reveal that the enhanced phenol adsorption strength is responsible for the superior catalytic efficiency. Furthermore, 1-cyclohexene-1-ol is an important intermediate. Its hydrogenation route and hence the final product are affected by the Ov density. This work opens a promising avenue to the rational design of advanced electrocatalysts for the upgrading of phenolic compounds.

Leaching NCM cathode materials of spent lithium-ion batteries with phosphate acid-based deep eutectic solvent.

Deep eutectic solvents (DESs) play an important role in efficient recovery of spent lithium-ion batteries (LIBs). In this study, we proposed an efficient and safe method by using a choline chloride-phenylphosphinic acid DES as a lixiviant for the leaching of LiNixCoyMnzO2 (NCM) cathode active materials of spent LIBs. The leaching conditions were optimized based on the leaching time, liquid-solid ratio, and leaching temperature. Under optimal experimental conditions, the leaching efficiencies of Li, Co, Ni, and Mn reached 97.7 %, 97.0 %, 96.4 %, and 93.0 %, respectively. The kinetics of the leaching process were well-fitted using the logarithmic law equation. The apparent activation energies for Li, Co, Ni, and Mn have been reported to be 60.3 kJ/mol, 78.9 kJ/mol, 99.3 kJ/mol, and 82.1 kJ/mol, respectively. UV-visible spectroscopy and Fourier transform infrared analysis revealed that the coordination configurations of Ni and Co in the leaching solution were octahedral and tetrahedral, respectively. In addition, the PO bond in phenylphosphinic acid was involved in coordination during leaching. This finding may provide an effective and safe approach for leaching valuable metals from spent LIBs.

Prediction of the Sound Absorption Coefficient of Three-Layer Aluminum Foam by Hybrid Neural Network Optimization Algorithm.

The combination of multilayer aluminum foam can have high sound absorption coefficients (SAC) at low and medium frequencies, and predicting its absorption coefficient can help the optimal structural design. In this study, a hybrid EO-GRNN model was proposed for predicting the sound absorption coefficient of the three-layer composite structure of the aluminum foam. The generalized regression neural network (GRNN) model was used to predict the sound absorption coefficient of three-layer composite structural aluminum foam due to its outstanding nonlinear problem-handling capability. An equilibrium optimization (EO) algorithm was used to determine the parameters in the neuronal network. The prediction results show that this method has good accuracy and high precision. The calculation result shows that this proposed hybrid model outperforms the single GRNN model, the GRNN model optimized by PSO (PSO-GRNN), and the GRNN model optimized by FOA(FOA-GRNN). The prediction results are expressed in terms of root mean square error (RMSE), absolute error, and relative error, and this method performs well with an average RMSE of only 0.011.

Novel processing strategies to enhance the bioaccessibility and bioavailability of functional components in wheat bran.

Dietary fiber, polysaccharides and phenols are the representative functional components in wheat bran, which have important nutritional properties and pharmacological effects. However, the most functional components in wheat bran exist in bound form with low bioaccessibility. This paper reviews these functional components, analyzes modification methods, and focuses on novel solid-state fermentation (SSF) strategies in the release of functional components. Mining efficient microbial resources from traditional fermented foods, exploring the law of material exchange between cell populations, and building a stable self-regulation co-culture system are expected to strengthen the SSF process. In addition, emerging biotechnology such as synthetic biology and genome editing are used to transform the mixed fermentation system. Furthermore, combined with the emerging physical-field pretreatment coupled with SSF strategies applied to the modification of wheat bran, which provides a theoretical basis for the high-value utilization of wheat bran and the development of related functional foods and drugs.

Incidence and related influencing factors of workplace violence among psychiatric nurses in China: A systematic review and Meta-analysis.

AIM: To analyze and integrate the incidence and its influencing factors on workplace violence among psychiatric nurses in China. BACKGROUND: Despite the fact that an increasing number of studies in China and elsewhere have focused on workplace violence among psychiatric nurses, there is presently no research to thoroughly explain the determining variables of violence faced by psychiatric nurses. DESIGN: A systematic review and meta-analysis was conducted. METHOD: PubMed, Embase, Cohrane Library, CNKI database, Wanfang database, and VIP database were used to search for English-language literature. The search deadline is June 15, 2021. The quality of the included literature was assessed, data was retrieved, and the meta-analysis was performed using Stata 16.0 software. RESULTS: A total of 19 works of literature, including 5926 patients, were included. Meta-analysis results showed that the incidence of workplace violence was about 78%[ES = 0.78, 95%CI(0.65,0.88)]. The possible factors for Chinese psychiatric nurses suffering from violence include gender, education, working years, whether they are an only child, age, height, working hours, and the form of employment. CONCLUSIONS: The incidence of workplace violence incidence among psychiatric nurses in China is high, and managers should employ individualized intervention methods based on the variables that influence it. IMPLICATIONS FOR NURSING MANAGEMENT: Managers should attach great importance to the occurrence of workplace violence. In training to prevent workplace violence, we should pay attention to the disparities in age and working abilities of nurses. Nurses should be trained in recognizing violent conduct, early warning indications, communication skills, and emergency response tactics. Managers should be able to schedule shifts in a flexible manner. Various techniques to enhance the nurse's working environment.

Screening and transcriptomic analysis of the ethanol-tolerant mutant Saccharomyces cerevisiae YN81 for high-gravity brewing.

Ethanol stress is one of the major limiting factors for high-gravity brewing. Breeding of yeast strain with high ethanol tolerance, and revealing the ethanol tolerance mechanism of Saccharomyces cerevisiae is of great significance to the production of high-gravity beer. In this study, the mutant YN81 was obtained by ultraviolet-diethyl sulfate (UV-DES) cooperative mutagenesis from parental strain CS31 used in high-gravity craft beer brewing. The ethanol tolerance experiment results showed that cell growth and viability of YN81 were significantly greater than that of CS31 under ethanol stress. The ethanol tolerance mechanisms of YN81 were studied through observation of cell morphology, intracellular trehalose content, and transcriptomic analysis. Results from scanning electron microscope (SEM) showed alcohol toxicity caused significant changes in the cell morphology of CS31, while the cell morphology of YN81 changed slightly, indicating the cell morphology of CS31 got worse (the formation of hole and cell wrinkle). In addition, compared with ethanol-free stress, the trehalose content of YN81 and CS31 increased dramatically under ethanol stress, but there was no significant difference between YN81 and CS31, whether with or without ethanol stress. GO functional annotation analysis showed that under alcohol stress, the number of membrane-associated genes in YN81 was higher than that without alcohol stress, as well as CS31, while membrane-associated genes in YN81 were expressed more than CS31 under alcohol stress. KEGG functional enrichment analysis showed unsaturated fatty acid synthesis pathways and amino acid metabolic pathways were involved in ethanol tolerance of YN81. The mutant YN81 and its ethanol tolerance mechanism provide an optimal strain and theoretical basis for high-gravity craft beer brewing.

Hydrophobicity Graded Gas Diffusion Layer for Stable Electrochemical Reduction of CO2.

To mitigate flooding associated with the gas diffusion layer (GDL) during electroreduction of CO2 , we report a hydrophobicity-graded hydrophobic GDL (HGGDL). Coating uniformly dispersed polytetrafluoroethylene (PTFE) binders on the carbon fiber skeleton of a hydrophilic GDL uniformizes the hydrophobicity of the GDL and also alleviates the gas blockage of pore channels. Further adherence of the PTFE macroporous layer (PMPL) to one side of the hydrophobic carbon fiber skeleton was aided by sintering. The introduced PMPL shows an appropriate pore size and enhanced hydrophobicity. As a result, the HGGDL offers spatial control of the hydrophobicity and hence water and gas transport over the GDL. Using a nickel-single-atom catalyst, the resulting HGGDL electrode provided a CO faradaic efficiency of over 83 % at a constant current density of 75 mA cm-2 for 103 h operation in a membrane electrode assembly, which is more than 16 times that achieved with a commercial GDL.

Novel melatonin-trientine conjugate as potential therapeutic agents for Alzheimer's disease.

Researchers continue to explore drug targets to treat the characteristic pathologies of Alzheimer's disease (AD). Some drugs relieve the pathological processes of AD to some extent, but the failed clinical trials indicate that multifunctional agents seem more likely to achieve the therapy goals for this neurodegenerative disease. Herein, a novel compound named melatonin-trientine (TM) has been covalently synthesized with the natural antioxidant compounds melatonin and the metal ion chelator trientine. After toxicological and pharmacokinetic verification, we elucidated the effects of intraperitoneal administration of TM on AD-like pathology in 6-month-old mice that express both the ß-amyloid (Aß) precursor protein and presenilin-1 (APP/PS1). We found that TM significantly decreased Aß deposition and neuronal degeneration in the brains of the APP/PS1 double transgenic mice. This result may be due to the upregulation of iron regulatory protein-2 (IRP2), insulin degrading enzyme (IDE), and low density lipoprotein receptor related protein 1 (LRP1), which leads to decreases in APP and Aß levels. Additionally, TM may promote APP non-amyloidogenic processing by activating the melatonin receptor-2 (MT2)-dependent signaling pathways, but not MT1. In addition, TM plays an important role in blocking γ-secretase, tau hyperphosphorylation, neuroinflammation, oxidative stress, and metal ion dyshomeostasis. Our results suggest that TM may effectively maximize the therapeutic efficacy of targeting multiple mechanisms associated with AD pathology.

Comparative Composition Structure and Selected Techno-Functional Elucidation of Flaxseed Protein Fractions.

This study aimed to comparatively elucidate the composition structure and techno-functionality of flaxseed protein isolate (FPI), globulin (FG), and albumin (FA) fractions. The results showed that FA possessed smaller particle dimensions and superior protein solubility compared to that of FG (p < 0.05) due to the lower molecular weight and hydrophobicity. FA and FG manifested lamellar structure and nearly spherical morphology, respectively, whereas FPI exhibited small lamellar strip structure packed by the blurring spheres. The Far-UV CD, FTIR spectrum, and intrinsic fluorescence confirmed more flexible conformation of FA than that of FG, followed by FPI. The preferential retention of free phenolic acids was observed for FA, leading to excellent antioxidant activities compared with that of FG in FPI (p < 0.05). FA contributed to the foaming properties of FPI, relying on the earlier interfacial adsorption and higher viscoelastic properties. FA displayed favorable emulsifying capacity but inferior stability due to the limited interfacial adsorption and deformation, as well as loose/porous interface. By comparison, an interlayer anchoring but no direct interface coating was observed for lipid droplets constructed by FG, thereby leading to preferable emulsion stability. However, FPI produced lipid droplets with dense interface owing to the effective migration of FA and FG from bulk phase, concomitant with the easy flocculation and coalescence. Thus, the techno-functionality of flaxseed protein could be tailed by modulating the retention of albumin fraction and specific phenolic acids.

Circadian protein CLOCK modulates regulatory B cell functions of nurses engaging day-night shift rotation.

Circadian rhythm proteins participate in regulating multiple physiological activities, including immune responses. The day-night shift rotation (DNSR) affects the circadian rhythm. The influence of circadian rhythm disturbance associated with DNSR on the regulatory functions of B cells remains to be studied. In this study, Blood samples were collected from 30 nurses engaging DNSR. The B cells were isolated from blood samples through magnetic cell separation. The regulatory function of IL-10 B cells (B10 cells) was evaluated using immunological assays. The results showed that the IL-10 expression was significantly reduced in B10 cells in nurses after DNSR. The capacity of inducing type 1 regulatory T cells (Tr1 cells) in B10 cells was down regulated. The circadian locomotor output cycles kaput (CLOCK) was increased in B10 cells, which was negatively correlated with the reduction of IL-10 expression in B10 cells. CLOCK formed a complex with c-Maf inducing protein (CMIP) to induce CMIP degradation; this restricted the IL10 gene transcription in B10 cells. B10 cells collected from nurses after DNSR were ineffective in suppressing T-cell proliferation and inducing Tr1 cells. In summary, DNSR affects the immune regulating function of B10 cells by disturbing the circadian rhythm.