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Anti-human proacrosin antibody inhibits the zona pellucida (ZP)-induced acrosome reaction of ZP-bound spermatozoa.

Veaute, Carolina; Liu, De Yi; Furlong, Laura I; Biancotti, Juan C; Baker, H W G; Vazquez-Levin, Mónica H.

Fertil Steril ; 93(7): 2456-9, 2010 May 01.

Article in English | MEDLINE | ID: mdl-19850289

ABSTRACT

The anti-acrosin monoclonal antibody AcrC5F10 inhibited proacrosin activation, proacrosin-human zona pellucida glycoprotein A (ZPA) binding, and the zona pellucida (ZP)-induced acrosome reaction of the ZP-bound spermatozoa but had no significant effect on sperm-ZP binding. These results suggest that proacrosin-acrosin may play an important role in the ZP-induced acrosome reaction of spermatozoa after primary binding to the ZP.

Subject(s)

Acrosin/immunology , Acrosome Reaction/drug effects , Antibodies/pharmacology , Enzyme Precursors/immunology , Spermatozoa/drug effects , Zona Pellucida/physiology , Acrosin/metabolism , Acrosome Reaction/immunology , Animals , CHO Cells , Cricetinae , Cricetulus , Egg Proteins/metabolism , Egg Proteins/pharmacology , Enzyme Activation/drug effects , Enzyme Precursors/metabolism , Female , Humans , Male , Membrane Glycoproteins/metabolism , Membrane Glycoproteins/pharmacology , Protein Binding , Receptors, Cell Surface/metabolism , Recombinant Proteins/metabolism , Spermatozoa/metabolism , Spermatozoa/physiology , Zona Pellucida/drug effects , Zona Pellucida Glycoproteins

ABSTRACT

Subject(s)

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