ABSTRACT
The culture of differentiated human airway epithelial cells allows the study of pathogen-host interactions and innate immune responses in a physiologically relevant in vitro model. As the use of primary cell culture has gained popularity the availability of the reagents needed to generate these cultures has increased. In this study we assessed two different media, Promocell and PneumaCult, during the differentiation and maintenance of well-differentiated primary nasal epithelial cell cultures (WD-PNECs). We compared and contrasted the consequences of these media on WD-PNEC morphological and physiological characteristics and their responses to respiratory syncytial virus (RSV) infection. We found that cultures generated using PneumaCult resulted in greater total numbers of smaller, tightly packed, pseudostratified cells. However, cultures from both media resulted in similar proportions of ciliated and goblet cells. There were no differences in RSV growth kinetics, although more ciliated cells were infected in the PneumaCult cultures. There was also significantly more IL-29/IFNλ1 secreted from PneumaCult compared to Promocell cultures following infection. In conclusion, the type of medium used for the differentiation of primary human airway epithelial cells may impact experimental results.
Subject(s)
Cell Differentiation , Culture Media/chemistry , Epithelial Cells/cytology , Epithelial Cells/virology , Nose/cytology , Primary Cell Culture/methods , Respiratory Syncytial Viruses/physiology , Cell Line , Child , Goblet Cells/cytology , HumansABSTRACT
OBJECTIVE: To develop and test the feasibility of a novel parent-inspired training intervention for hospital ward staff to improve communication with disabled children when inpatients. DESIGN: Training content and delivery strategies were informed by the iterative process of Intervention Mapping and developed in collaboration with parents of disabled children. SETTING: UK University Hospital children's ward. SUBJECTS: 80 medical, nursing, allied health professionals, clerical and housekeeping staff on a children's ward. METHODS: Themes identified in previous qualitative research formed the basis of the training. Learning objectives included prioritising communication, cultivating empathy, improving knowledge and developing confidence. Participant feedback was used to refine content and delivery. Intervention documentation adheres to the Template for Intervention Description and Replication checklist. RESULTS: Highlighting mandated National Health Service policies and involving the hospital Patient and Carer Experience Group facilitated management support for the training. Eighty staff participated in one of four 1-hour sessions. A paediatric registrar and nurse delivered sessions to mixed groups of staff. General feedback was very positive. The intervention, fully documented in a manual, includes videos of parent carers discussing hospital experiences, interactive tasks, small group discussion, personal reflection and intention planning. Generic and local resources were provided. CONCLUSION: It was feasible to deliver this new communication training to hospital ward staff and it was positively received. Early feedback was encouraging and indicates a commitment to behaviour change. Further piloting is required to establish the transferability of the intervention to other hospitals, followed by consideration of downstream markers to evaluate the effects on disabled children's inpatient experience. Organisational and cultural change is required to support individual behaviour change.
ABSTRACT
BACKGROUND: Communication is a fundamental part of health care, but can be more difficult with disabled children. Disabled children are more frequently admitted to hospital than other children. AIMS: To explore experiences of ward staff and families to identify barriers and facilitators to effective communication with disabled children whilst inpatients. DESIGN: This was an exploratory qualitative study. METHODS: We consulted 25 staff working on paediatric wards and 15 parents of disabled children recently admitted to those wards. We had difficulty in recruiting children and evaluating their experiences. Data were collected through interviews and focus groups. A thematic analysis of the data supported by the Framework Approach was used to explore experiences and views about communication. Emerging themes were subsequently synthesised to identify barriers and facilitators to good communication. RESULTS: Barriers to communication included time, professionals not prioritising communication in their role and poor information sharing between parents and professionals. Facilitators included professionals building rapport with a child, good relationships between professionals and parents, professionals having a family-centred approach, and the use of communication aids. CONCLUSIONS: Communication with disabled children on the ward was perceived as less than optimal. Parents are instrumental in the communication between their children and professionals. Although aware of the importance of communication with disabled children, staff perceived time pressures and lack of priority given to communicating directly with the child as major barriers.
Subject(s)
Attitude to Health , Disabled Children/psychology , Parents/psychology , Professional-Family Relations , Professional-Patient Relations , Adolescent , Adult , Child , Child, Preschool , Communication , England , Fathers , Female , Humans , Information Dissemination , Inpatients , Interviews as Topic , Male , Middle Aged , Mothers , PediatricsABSTRACT
We present the case of a boy born with a large macular, segmental vascular anomaly over the left face, initially diagnosed as a capillary malformation (port-wine stain) by the postnatal paediatric team. The vascular anomaly in the face then grew rapidly during the first few weeks of life and started to occlude the left eye, causing parental concerns about the infant's vision. A dermatological opinion established that the lesion was a segmental infantile haemangioma (IH). This, in combination with the posterior fossa malformation previously detected on antenatal scanning and confirmed by an MRI postnatally, satisfied the criteria for Posterior fossa abnormalities, Haemangiomas, Arterial abnormalities, Cardiac abnormalities and Eye abnormalities (PHACE) syndrome: a rare cutaneous neurovascular syndrome. This case highlights the diagnostic challenge posed by early phenotypes of haemangiomas as well as the importance of correctly diagnosing PHACE syndrome.
Subject(s)
Abnormalities, Multiple/diagnosis , Capillaries/abnormalities , Diagnostic Errors , Facial Neoplasms/diagnosis , Hemangioma, Capillary/diagnosis , Neurocutaneous Syndromes/diagnosis , Port-Wine Stain/diagnosis , Vascular Malformations/diagnosis , Arteries/abnormalities , Brain/abnormalities , Eye Abnormalities/diagnosis , Heart Defects, Congenital/diagnosis , Humans , Infant , Infant, Newborn , Male , Prenatal Diagnosis , SyndromeABSTRACT
OBJECTIVE: The variation in the anatomy of the iliopsoas tendon is important information for orthopaedic surgeons operating around the hip. The aim of this study was to identify the prevalence of bifid iliopsoas tendons in children on magnetic resonance imaging (MRI). METHODS: MRI hip and pelvis images of 50 sequential children aged 7-15 years were retrieved from our radiology database at the Evelina London Children's Hospital from 2007 to 2013. Included were 37 children with imaging of both hips and 13 children with imaging of one hip only. Therefore, our study was based on a total of 87 hips. RESULTS: At least 1 bifid tendon was noted in 13 children (26 %). Five children from a total of 37 (14 %) with both hips adequately imaged had bilateral bifid tendons. Among all 87 adequately imaged hips, 18 (21 %) were found to have two discrete distal iliopsoas tendons. CONCLUSIONS: Bifid iliopsoas tendon is noted anecdotally by surgeons but was only reported in scattered case reports and a few anatomical studies until very recently. Our finding is that a bifid iliopsoas tendon with two distinct tendinous components at the level of the hip joint is quite common. This has clinical significance, particularly in children's orthopaedic surgery when an adequate iliopsoas release is important.
ABSTRACT
The non-Langerhans histiocytoses (NLH) are a group of very rare disorders characterized by a predominantly cutaneous proliferation of non-Langerhans histiocytes. Involvement of the respiratory system is rare and more frequently affects the airways than the lung parenchyma. We describe a 44-year-old man with the progressive nodular form of NLH, with known cutaneous and hepatic involvement. High-resolution computed tomography performed for the investigation of his breathlessness revealed an interstitial pattern of disease closely resembling sarcoidosis. Lung biopsy showed non-Langerhans histiocytic infiltration of the lung parenchyma. Radiologists should be aware of NLH as a mimic of pulmonary sarcoidosis.
Subject(s)
Histiocytosis, Non-Langerhans-Cell/diagnostic imaging , Sarcoidosis , Tomography, X-Ray Computed/methods , Adult , Diagnosis, Differential , Humans , Lung/diagnostic imaging , MaleABSTRACT
Head and neck malignancy is rare in children. However, distinguishing malignant tumours from the more common and numerous benign causes of neck masses in childhood is crucial as many malignant conditions have an excellent prognosis with appropriate oncological management. Ultrasound, computed tomography and magnetic resonance imaging all have crucial roles in the diagnosis of head and neck malignancy in children and there is an emerging role for positron emission tomography, particularly in the management and follow-up of lymphoma. We describe the imaging appearances of the common malignant tumours arising in the extracranial head and neck in children, focusing on lymphoma, rhabdomyosarcoma and nasopharyngeal carcinoma. The clinical presentation and radiological appearances of benign tumours in the head and neck in children may overlap with those seen in malignant disease. We describe the imaging appearances of juvenile angiofibroma, vascular abnormalities involving the extracranial head and neck and cervical teratomas. Advances in both imaging techniques and cancer staging systems, many of the latter aimed at avoiding over-treatment and treatment-related complications, will lead to an increasingly central role for imaging in childhood head and neck cancer.
Subject(s)
Diagnostic Imaging/methods , Head and Neck Neoplasms/diagnosis , Angiofibroma/diagnostic imaging , Child , Head and Neck Neoplasms/diagnostic imaging , Humans , Lymphoma/diagnostic imaging , Lymphoma, Non-Hodgkin/diagnostic imaging , Magnetic Resonance Imaging , Nasopharyngeal Neoplasms/diagnostic imaging , Neoplasms/diagnostic imaging , Positron-Emission Tomography , Rhabdomyosarcoma/diagnostic imaging , Tomography, X-Ray Computed , Ultrasonography/methodsABSTRACT
Improvements in imaging technology allow exploitation of the dual blood supply of the liver to aid in the identification and characterisation of both malignant and benign liver lesions. Imaging techniques available include contrast enhanced ultrasound, computed tomography and magnetic resonance imaging. This review discusses the application of several imaging techniques in the diagnosis and staging of both hepatocellular carcinoma and cholangiocarcinoma and outlines certain characteristics of benign liver lesions. The advantages of each imaging technique are highlighted, while underscoring the potential pitfalls and limitations of each imaging modality.
Subject(s)
Cholangiocarcinoma/pathology , Liver Neoplasms/pathology , Cholangiocarcinoma/diagnostic imaging , Contrast Media , Hepatocytes/diagnostic imaging , Hepatocytes/pathology , Humans , Liver Neoplasms/diagnostic imaging , Liver Neoplasms/secondary , Magnetic Resonance Imaging , Neoplasm Metastasis/diagnostic imaging , Neoplasm Metastasis/pathology , Tomography, X-Ray Computed/methods , Ultrasonography/adverse effects , Ultrasonography/methodsSubject(s)
Abdomen, Acute/diagnostic imaging , Abdomen, Acute/etiology , Aortic Aneurysm, Abdominal/diagnostic imaging , Appendicitis/diagnostic imaging , Ascites/diagnostic imaging , Biliary Tract Diseases/diagnostic imaging , Gastrointestinal Diseases/diagnostic imaging , Humans , Kidney Diseases/diagnostic imaging , Pancreatitis/diagnostic imaging , UltrasonographySubject(s)
Genital Diseases, Female/diagnostic imaging , Genitalia, Female/diagnostic imaging , Female , Genital Diseases, Female/etiology , Genital Diseases, Female/pathology , Genitalia, Female/anatomy & histology , Genitalia, Female/pathology , Humans , Pregnancy , Pregnancy, Ectopic/diagnostic imaging , UltrasonographyABSTRACT
Metastases from bladder cancer to the bones of the hands or feet are rare and usually present after the diagnosis of the primary lesion has been made. This case report describes a 76-year-old man presenting with initial signs of infection of the right foot. Subsequent bone scan revealed multiple bony metastases and hydronephrosis raising the possibility of a primary bladder tumour that was later confirmed by urine cytology and fine needle aspiration of the foot.
Subject(s)
Bone Neoplasms/diagnostic imaging , Bone Neoplasms/secondary , Carcinoma, Transitional Cell/diagnostic imaging , Carcinoma, Transitional Cell/secondary , Urinary Bladder Neoplasms , Aged , Foot , Humans , Male , Radionuclide Imaging , Radiopharmaceuticals , Technetium Tc 99m Medronate/analogs & derivativesABSTRACT
Pheochromocytomas are tumors derived from chromaffin cells of the adrenal medulla that synthesize, store, metabolize, and usually, but not always, secrete catecholamines. Although pheochromocytomas are the cause of hypertension in only a small number of patients, they can precipitate life-threatening hypertension or cardiac arrhythmias caused by excessive and episodic catecholamine secretion. This article reviews the genetics, clinical presentation, and imaging of pheochromocytoma, with special emphasis on new positron emission tomographic radiopharmaceutical agents.