Osmotic regulators in cowpea beans plants under water deficiency.

Cowpea is a leguminous plant belonging to the fabaceae family cultivated in the North and Northeast regions of Brazil, with productive potential. Among the abiotic factors, water deficiency is one of the main environmental limitations that influence agricultural production in the world. The objective of this work was to study the relative water content and osmoregulators of cowpea plants subjected to water stress. The experiment was carried out in a greenhouse at the Universidade Federal Rural da Amazônia (UFRA, Belém, PA), cowpea plants BR-17 Gurguéia Vigna unguiculata (L.) Walp were used. The experimental design was completely randomized (DIC) in a 2 × 2 factorial scheme, two water conditions (control and water deficit) and two times of stress (four and six days of water suspension), with 7 replications, totaling 28 experimental units. The water deficit affected plants, causing a reduction in relative water content (69.98%), starch (12.84% in leaves and 23.48% in roots) and carbohydrates (84.34%), and an increase in glycine-betaine, sucrose (114.11% in leaves and 18.71% in roots) and proline (358.86%) at time 2. The relative water content was negatively affected by water conditions, with a decrease in relation to the interaction of the aerial part and the root system. Therefore, greater metabolic responses were noted in plants that were subjected to stress treatment at time 2 (6 days).

Influence of pen housing with litter on sanitary status, performance and carcass traits of growing rabbits / Influência do sistema de alojamento em piso com cama sobre o estado sanitário, desempenho e características de carcaça de coelhos em crescimento

The objective of this study was to evaluate the health status, productive performance and carcass traits of growing rabbits housed in pen with litter and conventional cage. At 35 d, 54 crossbred rabbits (New Zealand White vs. Botucatu) were randomly housed in pen with litter (6 pens, 6 rabbits/pen, 2.3 rabbits/m²) or cage (6 cages, 3 rabbits/cage, 8.3 rabbits/m²) and kept until the age of slaughter at 77 d. To simulate farm conditions, the rabbits that were housed in pen have not been previously adapted to this system. There was greater intensity of dirt and mucus occurrence in the rabbits housed in pens in first period, but the mucus occurrence practically disappeared in the later period. For performance, the rabbits housed in cages had a higher live weight at 56 d (1714 vs. 1506 g; P0.05) in the posterior period (56 to 77 d). There was no effect (P>0.05) of housing system for carcass yield, dissectible fat and hind leg yield. It may be suggested that rabbits adapt to the floor over time. Therefore, it is indicated the adoption of this system for growing rabbits.

O objetivo deste estudo foi avaliar o estado sanitário, o desempenho produtivo e as características de carcaça de coelhos em crescimento alojados em piso com cama e em gaiola convencional Aos 35 dias, 54 coelhos mestiços (Nova Zelândia Branco x Botucatu) foram alojados aleatoriamente em piso com cama (6 boxes; 6 coelhos/box; 2,3 coelhos/m²) ou em gaiola (6 gaiolas; 3 coelhos/gaiola; 8,3 coelhos/m²) e mantidos até a idade de abate, aos 77 dias. Para simular as condições de granja, os coelhos alojados em piso não foram previamente adaptados a este sistema. Houve maior intensidade de sujeira e ocorrência de muco nos coelhos alojados em piso no primeiro período, entretanto, esta ocorrência praticamente desapareceu no período posterior. Para o desempenho, os coelhos alojados em gaiola apresentaram maior peso vivo aos 56 dias (1714 vs. 1506 g; P0,05) os resultados de melhor desempenho no período posterior (56 a 77 dias). Não houve efeito (P>0,05) de sistema de alojamento para os rendimentos de carcaça referência, gordura dissecável e de pernil. Pode-se sugerir a ocorrência de adaptação dos coelhos ao piso, ao longo do tempo. Portanto, é indicada a adoção do sistema de alojamento em piso com cama para coelhos em crescimento.

Influence of pen housing with litter on sanitary status, performance and carcass traits of growing rabbits / Influência do sistema de alojamento em piso com cama sobre o estado sanitário, desempenho e características de carcaça de coelhos em crescimento

The objective of this study was to evaluate the health status, productive performance and carcass traits of growing rabbits housed in pen with litter and conventional cage. At 35 d, 54 crossbred rabbits (New Zealand White vs. Botucatu) were randomly housed in pen with litter (6 pens, 6 rabbits/pen, 2.3 rabbits/m²) or cage (6 cages, 3 rabbits/cage, 8.3 rabbits/m²) and kept until the age of slaughter at 77 d. To simulate farm conditions, the rabbits that were housed in pen have not been previously adapted to this system. There was greater intensity of dirt and mucus occurrence in the rabbits housed in pens in first period, but the mucus occurrence practically disappeared in the later period. For performance, the rabbits housed in cages had a higher live weight at 56 d (1714 vs. 1506 g; P<0.001), as well as BWG (46.4 vs. 36.7 g/d, P<0.001) and better FC (2.75 vs. 3.33; P<0.05) from 35 to 56 d. However, these animals did not show the best performance (P>0.05) in the posterior period (56 to 77 d). There was no effect (P>0.05) of housing system for carcass yield, dissectible fat and hind leg yield. It may be suggested that rabbits adapt to the floor over time. Therefore, it is indicated the adoption of this system for growing rabbits.(AU)

O objetivo deste estudo foi avaliar o estado sanitário, o desempenho produtivo e as características de carcaça de coelhos em crescimento alojados em piso com cama e em gaiola convencional Aos 35 dias, 54 coelhos mestiços (Nova Zelândia Branco x Botucatu) foram alojados aleatoriamente em piso com cama (6 boxes; 6 coelhos/box; 2,3 coelhos/m²) ou em gaiola (6 gaiolas; 3 coelhos/gaiola; 8,3 coelhos/m²) e mantidos até a idade de abate, aos 77 dias. Para simular as condições de granja, os coelhos alojados em piso não foram previamente adaptados a este sistema. Houve maior intensidade de sujeira e ocorrência de muco nos coelhos alojados em piso no primeiro período, entretanto, esta ocorrência praticamente desapareceu no período posterior. Para o desempenho, os coelhos alojados em gaiola apresentaram maior peso vivo aos 56 dias (1714 vs. 1506 g; P<0,001), bem como, maior ganho de peso diário (46,4 vs. 36,7 g/d; P<0,001) e melhor conversão alimentar (2,75 vs. 3,33; P<0,05), no período de 35 a 56 dias. Entretanto, estes animais não mantiveram (P>0,05) os resultados de melhor desempenho no período posterior (56 a 77 dias). Não houve efeito (P>0,05) de sistema de alojamento para os rendimentos de carcaça referência, gordura dissecável e de pernil. Pode-se sugerir a ocorrência de adaptação dos coelhos ao piso, ao longo do tempo. Portanto, é indicada a adoção do sistema de alojamento em piso com cama para coelhos em crescimento.(AU)

INFLUENCE OF PEN HOUSING WITH LITTER ON SANITARY STATUS, PERFORMANCE AND CARCASS TRAITS OF GROWING RABBITS

The objective of this study was to evaluate the health status, productive performance and carcass traits of growing rabbits housed in pen with litter and conventional cage. At 35 d, 54 crossbred rabbits (New Zealand White vs. Botucatu) were randomly housed in pen with litter (6 pens, 6 rabbits/pen, 2.3 rabbits/m²) or cage (6 cages, 3 rabbits/cage, 8.3 rabbits/m²) and kept until the age of slaughter at 77 d. To simulate farm conditions, the rabbits that were housed in pen have not been previously adapted to this system. There was greater intensity of dirt and mucus occurrence in the rabbits housed in pens in first period, but the mucus occurrence practically disappeared in the later period. For performance, the rabbits housed in cages had a higher live weight at 56 d (1714 vs. 1506 g; P

Female reproductive system morphology of crab-eating fox (Cerdocyon thous) and cryopreservation of genetic material for animal germplasm bank enrichment.

The sprawl of the urbanization and road network process without building ecological corridors contributes to the high mortality rates and a threat to the population decline of wild species such as the crab-eating fox. A strategy for the ex situ conservation is the study of the reproductive biology of the species and cryopreservation of their genetic heritage through the formation of an animal germplasm bank. This research is in accordance with the principles adopted by Brazilian College of Animal Experimentation. Reproductive systems of Cerdocyon thous females (n = 7) were examined macroscopically and microscopically by histological techniques and scanning electron microscopy. Gross features showed the shape of the ovaries was similar to a bean, and the elongated oviducts lengths were between 5 and 8 cm, with body of the uterus (3 cm) with long and narrow uterine horns (9-11 cm). The cervix was as a single annular conformation carrying out communication between the uterus and the vagina. The vagina has lengthened and circular muscle and the vulva with dense anatomical conformation with a quite pronounced clitoris. In addition, with regard to the establishment of a cell line (fibroblasts) for the gene bank enrichment, cells showed a low clonogenic capacity, especially when compared to domestic dogs, which can be explained by "in vitro" environment, age and diet of the animal. However, it was possible to create a bank of limited cell number. This study had morphological and preservationist character and aimed to help at long term in the conservation of wild animal's genetic resources.

Avaliação da confiabilidade entre dois observadores em exames citopatológico e imunocitoquímico de aspirado de medula óssea no diagnóstico da leishmaniose visceral canina / Reliability evaluation between two evaluators in the cytopathologic and immunocytochemical exams from bone marrow aspirate in the canine visceral leishmaniasis diagnosis

Visceral leishmaniasis is a zoonosis in which the dog appears as the main source of infection in urban areas. Its diagnosis is complex and the cytopathological exam is a fast and cheap alternative to parasite direct visualization and its sensitivity can be increased by immunocytochemistry, though with a higher cost. The accuracy of such methods is dependent on the microscopist's experience and therefore, this study evaluated the reliability of such techniques between two observers, from bone marrow aspirates of 50 dogs from an endemic area for the disease. The parasitological culture in Novy-MacNeal-Nicolle medium was used as the reference standard. Among the main findings, the sensitivities obtained by observers I and II were respectively 62.5% and 37.5%, while specificities were 81.1% and 100%. On immunocytochemistry evaluation, the sensitivity was 0% for both evaluators and the specificity 97.3% and 100%. The agreement between evaluators was weak (κ = 0.167) for the cytopathological test and it could not be evaluated for immunocytochemistry, for which there was no detection by the evaluator II. The agreements among the diagnostic methods and the standard reference for the observer I were reasonable (κ = 0.364) for cytopathological examination and bad (κ = -0.041) for immunocytochemistry. For observer II, such agreement could be assessed only for the cytopathological test, being moderate (κ = 0.497). The results point to the possible expertise difference between evaluators, with the evaluator II demonstrating greater experience when interpreting the citopathological test. Although there was the expected sensitivity increase with immunocytochemistry, the technique used in this study was not effective for the diagnosis of infection, regardless of the evaluator.(AU)

Avaliação da confiabilidade entre dois observadores em exames citopatológico e imunocitoquímico de aspirado de medula óssea no diagnóstico da leishmaniose visceral canina / Reliability evaluation between two evaluators in the cytopathologic and immunocytochemical exams from bone marrow aspirate in the canine visceral leishmaniasis diagnosis

Visceral leishmaniasis is a zoonosis in which the dog appears as the main source of infection in urban areas. Its diagnosis is complex and the cytopathological exam is a fast and cheap alternative to parasite direct visualization and its sensitivity can be increased by immunocytochemistry, though with a higher cost. The accuracy of such methods is dependent on the microscopist's experience and therefore, this study evaluated the reliability of such techniques between two observers, from bone marrow aspirates of 50 dogs from an endemic area for the disease. The parasitological culture in Novy-MacNeal-Nicolle medium was used as the reference standard. Among the main findings, the sensitivities obtained by observers I and II were respectively 62.5% and 37.5%, while specificities were 81.1% and 100%. On immunocytochemistry evaluation, the sensitivity was 0% for both evaluators and the specificity 97.3% and 100%. The agreement between evaluators was weak (κ = 0.167) for the cytopathological test and it could not be evaluated for immunocytochemistry, for which there was no detection by the evaluator II. The agreements among the diagnostic methods and the standard reference for the observer I were reasonable (κ = 0.364) for cytopathological examination and bad (κ = -0.041) for immunocytochemistry. For observer II, such agreement could be assessed only for the cytopathological test, being moderate (κ = 0.497). The results point to the possible expertise difference between evaluators, with the evaluator II demonstrating greater experience when interpreting the citopathological test. Although there was the expected sensitivity increase with immunocytochemistry, the technique used in this study was not effective for the diagnosis of infection, regardless of the evaluator.(AU)

Digestibilidade aparente dos nutrientes de dietas simplificadas baseadas em forragens para coelhos em crescimento / Apparent digestibility of nutrients of simplified diets based on forages for growing rabbits

Avaliaram-se os efeitos de dietas simplificadas à base de forragens sobre a digestibilidade aparente dos nutrientes em coelhos Nova Zelândia branco. As dietas experimentais foram: referência (REF), feno de alfafa (FAL), feno das folhas de rami (FRA), feno das folhas de amoreira (FAM) e feno do terço superior da rama da mandioca (FMA). A digestibilidade das dietas foi influenciada pelo tipo de alimento estudado; a FMA apresentou coeficientes de digestibilidade inferiores às demais dietas para todos os princípios nutritivos analisados. Para a dieta FAM, os coeficientes de digestibilidade aparente dos princípios nutritivos foram maiores (P<0,05) que os coeficientes das outras dietas estudadas. Os animais que ingeriram a dieta FAM apresentaram distúrbios digestivos e baixos consumos, com efeito negativo sobre os resultados. Os coeficientes de digestibilidade aparente das demais dietas estudadas foram semelhantes (P>0,05). Os valores estimados de energia digestível (kcal ED/kg MS) e proteína digestível ( por centoPD/MS) foram, respectivamente, para o feno de alfafa: 2285,27 e 16,04; feno das folhas de rami: 1857,88 e 16,37; feno das folhas de amoreira: 2838,48 e 15,12 e feno do terço superior da mandioca: 2155,55 e 10,57.(AU)

The effect of simplified diets based on forages on the apparent digestibility in white New Zealand rabbits was evaluated. The treatments were based on the following diets: reference (REF), hay of alfalfa (FAL), hay of rami leaves (FRA), hay of mulberry leaves (FAM) and hay of upper to 1/3 aereal part of cassava (FMA). The type of food affected the digestibility of the diets. The FMA diet showed low coefficients of digestibility in comparison to the other diets for all the analyzed nutrients. For the FAM diet the coefficients of apparent digestibility of the nutrients had higher values (P<0.05) than the coefficients of the other studied diets. The animals that ingested FAM diet showed digestive disorders and low consumption, with negative effect on the results. The coefficients of apparent digestibility of the remaining diets were similar (P>0.05). The estimated values of digestible energy (kcal DE/kg DM) and digestible protein ( percentDP/DM) were, respectively, 2285.27 and 16.04 for alfalfa hay, 1857.88 and 16.37 for hay of rami leaves, 2838.48 and 15.12 for hay of mulberry leaves and 2155.55 and 10.57 for hay of upper to 1/3 aereal part of cassava.(AU)

Digestibilidade aparente dos nutrientes de dietas simplificadas baseadas em forragens para coelhos em crescimento / Apparent digestibility of nutrients of simplified diets based on forages for growing rabbits

Avaliaram-se os efeitos de dietas simplificadas à base de forragens sobre a digestibilidade aparente dos nutrientes em coelhos Nova Zelândia branco. As dietas experimentais foram: referência (REF), feno de alfafa (FAL), feno das folhas de rami (FRA), feno das folhas de amoreira (FAM) e feno do terço superior da rama da mandioca (FMA). A digestibilidade das dietas foi influenciada pelo tipo de alimento estudado; a FMA apresentou coeficientes de digestibilidade inferiores às demais dietas para todos os princípios nutritivos analisados. Para a dieta FAM, os coeficientes de digestibilidade aparente dos princípios nutritivos foram maiores (P<0,05) que os coeficientes das outras dietas estudadas. Os animais que ingeriram a dieta FAM apresentaram distúrbios digestivos e baixos consumos, com efeito negativo sobre os resultados. Os coeficientes de digestibilidade aparente das demais dietas estudadas foram semelhantes (P>0,05). Os valores estimados de energia digestível (kcal ED/kg MS) e proteína digestível ( por centoPD/MS) foram, respectivamente, para o feno de alfafa: 2285,27 e 16,04; feno das folhas de rami: 1857,88 e 16,37; feno das folhas de amoreira: 2838,48 e 15,12 e feno do terço superior da mandioca: 2155,55 e 10,57.

The effect of simplified diets based on forages on the apparent digestibility in white New Zealand rabbits was evaluated. The treatments were based on the following diets: reference (REF), hay of alfalfa (FAL), hay of rami leaves (FRA), hay of mulberry leaves (FAM) and hay of upper to 1/3 aereal part of cassava (FMA). The type of food affected the digestibility of the diets. The FMA diet showed low coefficients of digestibility in comparison to the other diets for all the analyzed nutrients. For the FAM diet the coefficients of apparent digestibility of the nutrients had higher values (P<0.05) than the coefficients of the other studied diets. The animals that ingested FAM diet showed digestive disorders and low consumption, with negative effect on the results. The coefficients of apparent digestibility of the remaining diets were similar (P>0.05). The estimated values of digestible energy (kcal DE/kg DM) and digestible protein ( percentDP/DM) were, respectively, 2285.27 and 16.04 for alfalfa hay, 1857.88 and 16.37 for hay of rami leaves, 2838.48 and 15.12 for hay of mulberry leaves and 2155.55 and 10.57 for hay of upper to 1/3 aereal part of cassava.

Identification of deoxynivalenol, 3-acetyldeoxynivalenol and zearalenone in the galactose oxidase-producing fungus Dactylium dendroides.

The galactose oxidase-producing fungus Dactylium dendroides was re-identified as a Fusarium species. Fungi of this genus are well known for the production of mycotoxins. Verification of growth of this fungus on rice, corn and liquid medium described for the production of galactose oxidase is provided to determine whether the fungus could produce Fusarium toxins, namely, moniliformin, fusaric acid, fumonisin, zearalenone and the trichothecenes, deoxynivalenol, 3-acetyldeoxynivalenol, fusarenone, nivalenol, diacetoxyscirpenol, neosolaniol, and toxin T-2. Under the culture conditions used, deoxynivalenol, 3-acetyldeoxynivalenol and zearalenone were detected in the fungal culture medium. The finding is consistent with the hypothesis that the fungus is in fact a Fusarium species.