The ability of an organism to respond to nutritional stress can be a plastic character under the action of natural selection, affecting several characteristics, including life history and energy storage. The genus Drosophila (Diptera; Drosophilidae) presents high variability regarding natural resource exploration. However, most works on this theme have studied the model species D. melanogaster Meigen, 1830 and little is known about Neotropical drosophilids. Here we evaluate the effects of three diets, with different carbohydrate-to-protein ratios, on life history (viability and development time) and metabolic pools (triglycerides, glycogen, and total soluble protein contents) of three Neotropical species of Drosophila: D. maculifrons Duda, 1927; D. ornatifrons Duda, 1927, both of the subgenus Drosophila Sturtevant, 1939, and D. willistoni Sturtevant, 1916 of the subgenus Sophophora Sturtevant, 1939. Our results showed that only D. willistoni was viable on all diets, D. maculifrons was not viable on the sugary diet, while D. ornatifrons was barely viable on this diet. The sugary diet increased the development time of D. willistoni and D. ornatifrons, and D. willistoni glycogen content. Thus, the viability of D. maculifrons and D. ornatifrons seems to depend on a certain amount of protein and/or a low concentration of carbohydrate in the diet. A more evident effect of the diets on triglyceride and protein pools was detected in D. ornatifrons, which could be related to the adult attraction to dung and carrion baited pitfall as food resource tested in nature. Our results demonstrated that the evolutionary history and differential adaptations to natural macronutrient resources are important to define the amplitude of response that a species can present when faced with dietary variation.
Diet , Drosophila , Life History Traits , Animals , Drosophila/physiology , Energy Metabolism , Female , Male , Glycogen/metabolism , Dietary Proteins , Dietary Carbohydrates
The differential exploration of natural resources by Drosophila species has effects on fitness, with changes in life history and metabolic traits. There is a lack of research on the variation in these characters in different environments in Neotropical species of Drosophila. The purpose of this study was to evaluate the profile of life history traits, including viability, development time, and dry weight (as a measure of size), as well as the metabolic pools of triglyceride, glycogen, and protein, in populations from the southern and southeastern regions of Brazil of four Neotropical Drosophila species: D. willistoni, of the Sophophora subgenus, and D. mercatorum, D. maculifrons, and D. ornatifrons, which belong to the Drosophila subgenus. Life history and metabolic traits showed interpopulational variation in at least one species. When significant differences in life history parameters occurred, species of the same subgenus presented similar profiles, i.e., southern populations were larger, less viable, and showed longer development time. This was also observed for triglyceride. However, for the other two metabolic pools (glycogen and total proteins), D. maculifrons and D. ornatifrons presented inverse patterns to the other two species, with the highest values in southeastern populations and the lowest in southern populations. These populational variations indicate plasticity of the examined life history traits, which allows distinctive responses to different environmental conditions shared by speciesof the same subgenus. Nevertheless, interspecific comparisons did not reflect phylogenetic relationships, with the highest viability being found for D. willistoni and D. mercatorum, which is probably correlated to the ability of these species to explore a broader variety of habitats. On the other hand, the storage capability of metabolic pools seems to be species specific, determined by the adaptive history to the quality and availability of resources, with D. mercatorum (low) and D. ornatifrons (high) having opposing capacities to store metabolites from their diets.
Recently renamed, Psalidodon scabripinnis populations of Serra da Mantiqueira, previously known as Astyanax scabripinnis have been deeply studied in the last years. These populations are small and isolated and occur very close to the watershed between Paraíba do Sul River basin and Upper Paraná River basin, in Serra da Mantiqueira region in the Atlantic Rainforest. These conditions arouse the interest in knowing theor genetic conservation status and how they responded to the separation between the two rivers basins. Therefore, we accessed the genetic diversity of five P. scabripinnis populations of this region with microsatellites and mitochondrial data. The results showed a complex structure pattern that doesn't match the simple basin separation and a reasonably conservation status when compared with other populations of the same family or with similar natural history.
Drosophila sturtevanti is a widely distributed Neotropical species. In South America, it is abundant and adapted to different phytophysiognomies of the Atlantic Forest biome. Reproductive, chromosomal and enzymatic studies have indicated the existence of a differentiation among D. sturtevanti populations. In this work, the level of genetic diversity and the population genetic structure were analyzed using four population groupings. One hundred and twenty-six D. sturtevanti males collected from nine forest fragments were analyzed for 11 species-specific microsatellite loci. A total of 109 alleles, ranging from 2 to 16 alleles per locus, were detected. The highest mean observed heterozygosity -H O was estimated in samples from the largest collection areas, and the lowest H O was from a population where fire events are common. A low molecular variation, around 3% among populations and negative among groups, an absence of genetic and geographic correlations and a moderate genetic differentiation -F ST = 0.0663 -indicated that D. sturtevanti is not strongly structured. Besides no overall genetic and geographic distance correlation, the pair of closest geographically populations Matão and Nova Granada showed the lower differentiation through F ST, DC and a Neighbor Joining tree. Ribeirão da Ilha -RDI, an isolated insular population, was the most differentiated according to F ST, DC and a cluster-based Bayesian analysis. The isolation of RDI that resulted in significant divergence could be ancient, because of sea level regressions/transgressions, or more recently via founder effect/genetic drift by anthropic action carrying D. sturtevanti hosts from continent to island. This work is important for understanding the genetic variability distribution of a Neotropical forest-dwelling Drosophila species using for the first time, a wide population distribution approach.
Access the genetic variability of endangered and isolated populations has become an important conservation tool. Astyanax scabripinnis is a well-known fish model for genetic studies, forming very isolated populations in headwaters. Besides that, this species frequently presents supernumerary chromosomes, which elevates the interest on genetic studies. Genetic diversity of an Astyanax scabripinnispopulation from the Atlantic Forest (Serra da Mantiqueira region, Brazil) was assessed with microsatellite markers for the first time. Since microsatellite markers are not described for this species, we tested markers described for a related species for transferability to A. scabripinnis. Six polymorphic loci were sufficiently reliable for population genetic analysis. We found that this population passed through a recent bottleneck because of the presence of an excess of heterozygotes, low allelic diversity, heterozygosity excess, and small effective population size. Individuals with and without B chromosomes were previously identified in this population and our study found private alleles in the individuals without B chromosomes. Furthermore, when individuals without B chromosomes were removed from the analysis, the population did not present heterozygosity excess, suggesting that the bottleneck event was driven by individuals with B chromosomes. Our results provide an insight into the value of microsatellite markers as molecular tools and is the first genetic study using molecular data of A. scabripinnis from this area.
Characidae/genetics , Microsatellite Repeats , Genetic Variation
Drosophila mojavensis normally breeds in necrotic columnar cactus, but they also feed and breed in Opuntia fruit (prickly pear) which serves as a seasonal resource. The prickly pear fruits (PPFs) are much different chemically from cacti, mainly in their free sugars and lipid content, raising the question of the effects of this seasonal shift on fitness and on gene expression. Here we reared 3 isofemale strains of D. mojavensis collected from different parts of the species' range on semi-natural medium of either cactus or PPF and measured the development time, survival, body weights, and desiccation resistance. All these parameters were affected by diet and by interaction with strain and or sex. Interestingly, however, there appear to be tradeoffs: flies developed faster in prickly pear and the emerging adults were heavier, but those having grown in cactus were more resistant to desiccation. We also evaluated the gene expression of emerging male and female adult flies using RNA-Seq. While more genes were down-regulated in PPF than up-regulated in both sexes, the sexes did differ in expression patterns. The majority of the genes that were preferentially expressed comparing PPF versus cactus underlie metabolism. Genes involved with carbohydrate and lipid metabolism, as well as with the amino acid serine, and their relationship to growth and development reflect the ways in which these dietary differences affect the flies.
Drosophila/physiology , Opuntia/parasitology , Animals , Diet , Drosophila/genetics , Drosophila/growth & development , Female , Gene Expression Regulation, Developmental , Male , North America , Seasons , Transcription, Genetic
Luciana Paes de Barros Machado, Natalia Silva Alves, Jaqueline de Oliveira Prestes, Gabriela Ronchi Salomón, Daiane Biegai, Thais Wouk, and Rogério Pincela Mateus (2017) Esterases are a diversified group of isozymes that performs several metabolic functions in Drosophila. In the D. repleta group, this class of enzymes was well described in cactophilic species, existing a lack of studies considering substrate speci city and life cycle expression in the non-cactophilic species. The larvae of cactophilic species of the D. repleta group develop in rotting cacti cladodes, but adults are generalists. Thus, different patterns expression can be found for esterases throughout development. In this work we analyzed esterase pro le and substrate speci city during development, and genetic variability aspects in D. mercatorum pararepleta, a non-cactophilic and generalist species of D. repleta group that was understudied hitherto. Samples of 3rd (F3) and 104th (F104) generations of three D. mercatorum pararepleta strains, obtained after collections in xerophytic enclaves of southeastern Brazil (ITI and SER in São Paulo state and RIP in Paraná state), and of D33 strain (obtained from Cristalina-GO, Midwest of Brazil, and established in the laboratory in 1987) were analyzed. Eight esterase loci, EST-1 to EST-8, were detected. EST-1 and EST-2 were adult exclusive. Only EST-3 and EST-8 were monomorphic; all the others presented between two (EST-6) and six (EST-7) alleles. EST-7 was the only dimeric locus and also the only one that showed to be a preferably ß-esterase regarding affinity to α- and ß-naphthyl acetates as substrates. The other seven loci were divided into three classes: α-esterase exclusive (EST-2); preferably α-esterase (EST-3, EST-4, EST-5 and EST-8); and α/ß-esterase (EST-1 and EST-6). The EST-3, EST-5 and EST-6 loci were not detected in all samples, suggesting that they could have become pseudogenes due to the mutation accumulation after the gene duplication. The allele frequency of EST-7 locus, which showed the highest number of alleles, in adults of D33 and SER-F3 evidenced a higher variability and diversity in the oldest strain (six alleles, Ho = 0.46) than in the youngest ( five alleles, Ho = 0.26). Moreover, the analysis of SER-F104 revealed that this locus became monomorphic. The higher variability in the strain established in the laboratory at least two decades ago, together with the allele fixation in the SER-F104, indicate that the SER strain probably suffered a more severe action of founder effect/bottleneck when it was established in the laboratory and, therefore, even if the maintenance afterwards was performed using a high number of individuals, it did not assured the conservation of the existing genetic variability.
The behavioral biology has a central role in evolutionary biology mainly because the antagonistic relations that occur in the sexual reproduction. One involves the effect of reproduction on the future life expectation. In this scenario, changes in male operational sex ratio could lead to an increase in mortality due to costs associated with excessive courtship and mating displays. Thus, this work experimentally altered the male sex ratio of Drosophila melanogaster Meigen, 1830, to determine its impact on mortality. The results indicated that mortality increases as the sex ratio changes, including modifications in the survivorship curve type and in the curve concavity, measured by entropy.
A biologia comportamental tem um papel central na biologia evolutiva principalmente pelas relações antagônicas que ocorrem na reprodução sexuada. Uma destas relações envolve o efeito da reprodução sobre a expectativa de vida futura. Neste cenário, alterações na razão sexual operacional de machos podem levar a um aumento na mortalidade por causa dos custos associados com o excesso de displays de corte e cópulas. Neste sentido este trabalho alterou experimentalmente a razão sexual em machos de Drosophila melanogaster Meigen, 1830, para determinar os efeitos em termos de mortalidade. Os resultados indicam que a mortalidade aumenta a medida que a razão sexual se enviesa incluindo alterações no tipo de curva de sobrevivência e da concavidade da curva, medida pela entropia.
A method that allows the measure of molecular weight of two well-known and closely related esterases from Drosophila mojavensis and its sibling species, D. arizonae, is here described, using native polyacrylamide gel electrophoresis at several concentrations, applying Fergunson´s principles. These enzymes, namely EST-4 and EST-5, presented molecular weight values between 81 and 91 kDa. In spite of their distinct expression pattern through the insect's life cycle, they showed properties of isoenzymes codified by distinct structural genes, supporting the hypothesis of a rather recent gene duplication event that generated both in D. mojavensis and D. arizonae, as well as in other species of repleta group. The method is simple and adequate to be applied to preliminary molecular weight determination of other enzymes without any previous purification procedure.
Neste trabalho, um método que permite a estimativa do peso molecular de duas esterases conhecidas e intimamente relacionadas, encontradas em Drosophila mojavensis e sua espécie aparentada D. arizonae, é descrito. Este método é realizado utilizando a técnica de eletroforese em diferentes concentrações de gel e aplicando os princípios de Fergunson. As enzimas, denominadas EST-4 e EST-5, apresentaram pesos moleculares entre 81 e 91 kDa. Apesar de seus padrões diferenciados de expressão durante o ciclo de vida do inseto, elas demonstraram propriedades de enzimas codificadas por genes estruturais distintos, corroborando a hipótese de um evento de duplicação gênica recente que gerou ambas em D. mojavensis e D. arizonae, bem como em outras espécies do grupo repleta. O método proposto é simples e adequado para ser utilizado em estimativas preliminares de determinação de pesos moleculares de outras enzimas sem haver a necessidade de um procedimento prévio de purificação.
The taxonomy of the genus Hypostomus in the upper Rio Paraná basin presents some very complex issues. For this reason, an allozyme electrophoresis survey of 10 populations of Hypostomus from the Rio Corumbá basin was performed, revealing 25 loci from 14 enzyme systems. Allozyme data revealed diagnostic genetic markers for the four nominal species analyzed (Hypostomus ancistroides, H. iheringi, H. margaritifer, and H. regani) and for six possible undescribed species. The mean expected heterozygosity of the populations ranged from 0.009 in H. iheringi to 0.099 in Hypostomus sp. 4. The genetically most similar populations (I = 0.954) were H. iheringi and Hypostomus sp. 2; the most divergent (D = 0.839) were H. iheringi and H. ancistroides.
Catfishes/classification , Isoenzymes/genetics , Animals , Brazil , Catfishes/genetics , Heterozygote , Isoenzymes/metabolism , Species Specificity
In an allozyme electrophoresis survey of 15 hypostomine species from the Itaipu Hydroelectric Reservoir, 25 loci from 14 enzyme systems were scored. Allozyme data allowed recording diagnostic genetic markers for all species analyzed and for some species groups within Hypostomus, a taxon which is taxonomically still unresolved in the Upper Rio Paraná basin. The mean expected heterozygosity of the species was considerably variable and hypotheses to tentatively explain this variation are discussed. A cladogram based upon the allelic frequencies of the species analyzed was produced by the continuous maximum likelihood method: Rhinelepis aspera and M. parananus were separated from the species of Hypostominae by a long branch length. Pterygoplichthys anisitsi was the sister of all the representatives of the genus Hypostomus. Within Hypostomus, two main clades were produced: in the first, H. cochliodon was the sister of the species comprising the H. plecostomus group, and in the second, the tree showed the following relationships: (H. albopunctatus (H. regani + Hypostomus sp. 3) + (H. margaritifer (H. microstomus (Hypostomus sp. 1 (H. ternetzi + Hypostomus sp. 2)). Hypostomus ternetzi and Hypostomus sp. 2 are referred to here as representatives of the H. ternetzi group.
Catfishes/genetics , Isoenzymes/genetics , Animals , Brazil , Catfishes/classification , Electrophoresis , Genetic Variation , Heterozygote , Phylogeny , Species Specificity
