1.
Methods Mol Biol
; 1389: 279-92, 2016.
Article
in English
| MEDLINE
| ID: mdl-27460253
ABSTRACT
Hemoglobin S polymerization under hypoxic conditions in sickle cell disorders causes characteristic shape changes to human red blood cells. Previous sickling assays used to investigate the efficacy of novel agents to treat these disorders are laborious and observer dependent. Here, we describe a partially automated, high-throughput sickling assay using imaging flow cytometry.