ABSTRACT
Flexible textile displays can be revolutionary for information transmission at any place and any time. Typically, textile displays are fabricated by traditional rigid electronics that sacrifice mechanical flexibility of devices or by flexible electronics that do not have an appropriate choice to arbitrarily control single pixels. This work reports on an electroluminescent fabric woven by ultrastretchable fibers (electroluminescent fibers up to 400% stretch, electrode fibers up to 250% stretch), which can exhibit the pixel-based arbitrarily controllable pattern display by a mobile phone application. To realize ultrastretchability, we made these fibers by encapsulating liquid metals on a polyurethane core (high elasticity). To realize arbitrary control, the design shows a plain-woven structure comprising ZnS-based electroluminescent fibers and perpendicular electrode fibers. The cross-points between the electroluminescent fiber and the electrode fiber form pixels that can be switched on or off independently and can further form the pixel-based arbitrarily controllable pattern display. By doping with different elements, ZnS-based electroluminescent fibers can emit green, blue, or yellow lights. Meanwhile, the fabrication of these fibers employs dip-coating, a scalable manufacturing method without high temperature or vacuum atmosphere. These fabrics show great potential in a wide range of applications such as wearable electronic devices, healthcare, and fashion design.
ABSTRACT
In eukaryotes, conserved mechanisms ensure that cell growth is coordinated with nutrient availability. Overactive growth during nutrient limitation ("nutrient-growth dysregulation") can lead to rapid cell death. Here, we demonstrate that cells can adapt to nutrient-growth dysregulation by evolving major metabolic defects. Specifically, when yeast lysine-auxotrophic mutant lys- encountered lysine limitation, an evolutionarily novel stress, cells suffered nutrient-growth dysregulation. A subpopulation repeatedly evolved to lose the ability to synthesize organosulfurs (lys-orgS-). Organosulfurs, mainly reduced glutathione (GSH) and GSH conjugates, were released by lys- cells during lysine limitation when growth was dysregulated, but not during glucose limitation when growth was regulated. Limiting organosulfurs conferred a frequency-dependent fitness advantage to lys-orgS- by eliciting a proper slow growth program, including autophagy. Thus, nutrient-growth dysregulation is associated with rapid organosulfur release, which enables the selection of organosulfur auxotrophy to better tune cell growth to the metabolic environment. We speculate that evolutionarily novel stresses can trigger atypical release of certain metabolites, setting the stage for the evolution of new ecological interactions.
Subject(s)
Adaptation, Physiological/genetics , Lysine/pharmacology , Metabolic Networks and Pathways/drug effects , Nutrients/pharmacology , Saccharomyces cerevisiae/metabolism , Autophagy/drug effects , Autophagy/genetics , Biological Evolution , Glucose/metabolism , Glucose/pharmacology , Lysine/deficiency , Metabolic Networks and Pathways/genetics , Nitrogen/metabolism , Nitrogen/pharmacology , Nutrients/metabolism , Ribosomes/drug effects , Ribosomes/metabolism , Saccharomyces cerevisiae/drug effects , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/growth & development , Sirolimus/pharmacology , Stress, PhysiologicalABSTRACT
Quantitative modeling is useful for predicting behaviors of a system and for rationally constructing or modifying the system. The predictive power of a model relies on accurate quantification of model parameters. Here, we illustrate challenges in parameter quantification and offer means to overcome these challenges, using a case example in which we quantitatively predict the growth rate of a cooperative community. Specifically, the community consists of two Saccharomyces cerevisiae strains, each engineered to release a metabolite required and consumed by its partner. The initial model, employing parameters measured in batch monocultures with zero or excess metabolite, failed to quantitatively predict experimental results. To resolve the model-experiment discrepancy, we chemically identified the correct exchanged metabolites, but this did not improve model performance. We then remeasured strain phenotypes in chemostats mimicking the metabolite-limited community environments, while mitigating or incorporating effects of rapid evolution. Almost all phenotypes we measured, including death rate, metabolite release rate, and the amount of metabolite consumed per cell birth, varied significantly with the metabolite environment. Once we used parameters measured in a range of community-like chemostat environments, prediction quantitatively agreed with experimental results. In summary, using a simplified community, we uncovered and devised means to resolve modeling challenges that are likely general to living systems.
Subject(s)
Microbial Interactions/genetics , Models, Biological , Saccharomyces cerevisiae/metabolism , Coculture Techniques , Computer Simulation , Metabolic Engineering/methods , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/growth & developmentABSTRACT
Stretchable, biocompatible devices can bridge electronics and biology. However, most stretchable conductors for such devices are toxic, costly, and regularly break/degrade after several large deformations. Here we show printable, highly stretchable, and biocompatible metal-polymer conductors by casting and peeling off polymers from patterned liquid metal particles, forming surface-embedded metal in polymeric hosts. Our printable conductors present good stretchability (2,316 S/cm at a strain of 500%) and repeatability (ΔR/R <3% after 10,000 cycles), which can satisfy most electrical applications in extreme deformations. This strategy not only overcomes large surface tension of liquid metal but also avoids the undesirable sintering of its particles by stress in deformations, such that stretchable conductors can form on various substrates with high resolution (15 µm), high throughput (â¼2,000 samples/hour), and low cost (one-quarter price of silver). We use these conductors for stretchable circuits, motion sensors, wearable glove keyboards, and electroporation of live cells.