Weed Management and Crop Establishment Methods in Rice (Oryza sativa L.) Influence the Soil Microbial and Enzymatic Activity in Sub-Tropical Environment.

Weed management has become the most important and inevitable aspect of crop management for achieving a higher rice yield. Nowadays, chemical herbicide application has become a popular practice for managing weeds in different rice cultures. However, herbicide application can have qualitative and quantitative impacts on soil microorganisms and soil enzymes, particularly in the case of new herbicide molecules and their indiscriminate use for a longer period. Further, different rice establishment methods also play a significant role in soil microbial population dynamics as well as soil biological properties. Keeping these in view, a field experiment was conducted at the Agronomy Main Research Farm, Orissa University of Agriculture and Technology (OUAT), India, during the kharif season of 2016 and 2017, on the impact of crop establishment methods and weed management practices on soil microbial and enzymatic status. The field experiment was laid out in a split-plot design with three replications with four crop establishment methods in the main plot, viz., M1, Direct Seeded Rice (DSR); M2, Wet Seeded Rice (WSR); M3,Unpuddled Transplanted Rice (NPTR); M4, Puddled Transplanted Rice (PTR), and six weed management practices in the sub-plot, viz., W1, Weedy check; W2, Bensulfuron methyl 0.6% + Pretilachlor 6% (pre-emergence (PE)) 0.660 kg ha-1 + Hand weeding (HW) at 30 days after sowing/transplanting (days after sowing/transplanting (DAS/T)); W3, Bensulfuron methyl 0.6% + Pretilachlor 6% (PE) 0.495 kg ha-1 + HW at 30 DAS/T; W4, Bensulfuron methyl 0.6% + Pretilachlor 6% (PE) 0.495 kg ha-1 + Bispyribac-Sodium (post-emergence(POE)) 0.025 kg ha-1 at 15 DAS/T; W5, Cono weeding (CW) at 15 DAS/T + hand weeding 30 DAS/T, and W6, Brown manuring/Green manuring. The initial decline in the microbial population was observed due to herbicide application in NPTR and PTR up to 7 DAS/T and then it increased up to 28 DAS/T. There was a reduction in soil microbial and enzymatic status after the application of herbicides Bensulfuron methyl 0.6% + Pretilachlor 6% (PE) and Bispyribac-Sodium (POE) that again followed an upward graph with crop age. Significant variation in enzymatic activity and the microbial count was also observed among treatments involving crop establishment methods. The study revealed that improved microbial population and enzyme activity were noted in unpuddled transplanted rice under organic weed management due to favorable conditions, and chemical weed control initially affected microbial population and activities.

Evaluation of efficient glucose release using sodium hydroxide and phosphoric acid as pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.: A fast growing tree legume.

Sesbania grandiflora (L.) Pers. is one of the fast growing tree legumes having the efficiency to produce around 50tha-1 above ground dry matters in a year. In this study, biomass of 2years old S. grandiflora was selected for the chemical composition, pretreatments and enzymatic hydrolysis studies. The stem biomass with a wood density of 3.89±0.01gmcm-3 contains about 38% cellulose, 12% hemicellulose and 28% lignin. Enzymatic hydrolysis of pretreated biomass revealed that phosphoric acid (H3PO4) pretreated samples even at lower cellulase loadings [1 Filter Paper Units (FPU)], could efficiently convert about 86% glucose, while, even at higher cellulase loadings (60FPU) alkali pretreated biomass could convert only about 58% glucose. The effectiveness of phosphoric acid pretreatment was also supported by X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FTIR) analysis.

Chemical composition, pretreatments and saccharification of Senna siamea (Lam.) H.S. Irwin & Barneby: An efficient biomass producing tree legume.

Protocols were developed for efficient release of glucose from the biomass of Senna siamea, one of the highly efficient biomass producing tree legumes. Composition of mature, 1year and 2years coppice biomass were analysed. For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of ß-glucosidase per gram of glucan. Of the biomass of different particle sizes (40-200mesh) used for saccharification, 40-60mesh shown the maximum glucose release. COSLIF pretreated mature, 1year and 2years coppice biomass showed equivalent glucose release profiles.