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1.
PLoS One ; 18(9): e0291716, 2023.
Article in English | MEDLINE | ID: mdl-37713404

ABSTRACT

PURPOSE: To investigate the presence of amyloidosis-related ocular findings in patients who received domino liver transplantation from ATTRv amyloidosis donors. METHODS: We reviewed the ocular findings in patients who had previously undergone domino liver transplantation and received ophthalmologic examinations between January 2009 and March 2023. The presence of amyloidosis-related ocular findings was retrospectively assessed by two ophthalmologists. RESULTS: During the study period, a total of 7 patients with 14 eyes were examined. All patients were considered as acquired ATTRv amyloidosis. The mean age at the final visit was 64.6±8.4 years (52-75 years), and the mean time since domino liver transplantation was 167.6±76.2 months (69-257 months). The two evaluators' assessments for amyloidosis-related ocular findings were completely identical. No amyloid fibril deposition was observed in the pupil, lens, or vitreous. Five patients (10 eyes) had a Schirmer test result of 5mm or less than 5 mm, and four patients with a total of 8 eyes underwent fluorescein angiography and indocyanine green angiography, and no evidence of retinal amyloid angiopathy was found on fluorescein angiography. However, three patients with 6 eyes showed choroidal amyloid angiopathy on indocyanine green angiography. CONCLUSION: While cases of choroidal amyloid angiopathy were observed, serious amyloidosis-related ocular complications such as vitreous opacity or secondary glaucoma did not occur even in the long term after domino liver transplantation.


Subject(s)
Amyloidosis , Indocyanine Green , Aged , Humans , Middle Aged , Fluorescein Angiography , Pupil , Retrospective Studies
2.
Sci Rep ; 13(1): 12755, 2023 08 07.
Article in English | MEDLINE | ID: mdl-37550352

ABSTRACT

This study reports the long-term results of trabeculectomy (LEC) for secondary glaucoma in hereditary transthyretin (ATTRv) amyloidosis patients and its correlation with prior vitrectomy. A retrospective case series was conducted involving 31 consecutive eyes of 20 ATTRv amyloidosis patients who underwent LEC between 2007 and 2020. The mean follow-up period was 73.2 ± 37.0 months (range: 20-181 months). Postoperative intraocular pressures (IOPs) were evaluated based on the following criteria: (a) IOP between 6 and 21 mmHg without additional glaucoma surgeries, except for laser suture lysis, (b) IOP between 6 and 15 mmHg without additional glaucoma surgeries, except for laser suture lysis, and (c) IOP between 6 and 21 mmHg without additional glaucoma surgeries, except for needling and laser suture lysis. Kaplan-Meier analysis revealed survival rates after LEC of 0.52 at 36 months, 0.42 at 60 months, and 0.25 at 84 months under criterion (a); 0.49 at 36 months, 0.27 at 60 months, and 0.11 at 84 months under criterion (b); and 0.76 at 36 months, 0.71 at 60 months, and 0.65 at 84 months under criterion (c). Eyes with a history of small gauge transconjunctival vitrectomy (SGTV) exhibited a tendency towards lower survival rates, although no statistically significant difference was observed (log-rank test; p = 0.193 under criterion (a) and p = 0.0553 under criterion (b)). Our findings suggest that LEC and additional needling procedures can provide some control over IOP; however, the overall postoperative outcomes of LEC for ATTRv amyloidosis remain unsatisfactory, even in the era of SGTV with reduced conjunctival scarring.


Subject(s)
Amyloid Neuropathies, Familial , Glaucoma , Trabeculectomy , Humans , Trabeculectomy/methods , Retrospective Studies , Glaucoma/surgery , Amyloid Neuropathies, Familial/complications , Amyloid Neuropathies, Familial/genetics , Amyloid Neuropathies, Familial/surgery , Intraocular Pressure , Treatment Outcome , Follow-Up Studies
3.
Jpn J Ophthalmol ; 67(1): 84-90, 2023 Jan.
Article in English | MEDLINE | ID: mdl-36441264

ABSTRACT

PURPOSE: To report surgical outcomes of a Microhook ab interno trabeculotomy (µLOT) procedure for glaucoma secondary to hereditary transthyretin amyloidosis (ATTRv). STUDY DESIGN: Retrospective case series. METHODS: Medical records of patients with glaucoma secondary to ATTRv with transthyretin Val30Met variant, who underwent µLOT, were retrospectively reviewed. Surgical success was categorized according to the postoperative intraocular pressures (IOPs, mmHg) as follows: (a) 6 ≤ IOP ≤ 21; (b) 6 ≤ IOP ≤ 18; and (c) 6 ≤ IOP ≤ 15, without light perception loss or additional glaucoma surgery. Secondary outcomes were glaucoma medication scores and postoperative complications. RESULTS: This study included 18 eyes (13 patients, 6 men). The mean follow-up period was 25.2±9.8 months (7-38 months). Kaplan-Meier analysis indicated success rates of (a) 1.00 at 6, 1.00 at 12, and 0.43 at 24 months; (b), 1.00 at 6, 0.93 at 12, and 0.43 at 24 months; (c) 0.94 at 6, 0.75 at 12, and 0.27 at 24 months after operation. Postoperative IOPs were significantly reduced from the baseline of 25.2±5.8 mmHg to 11.5±2.7 at 3, 12.3±4.1 at 6, and 13.8±3.9 at 12 months (Dunnett's test). Medication scores were also improved at 3 and 6 months but without a significant reduction at 12 months. There were no severe complications requiring surgical intervention except for additional glaucoma surgery. CONCLUSION: µLOT for secondary glaucoma in ATTRv is safe and effective 1 year after surgery, but the effects diminish after 2 years.


Subject(s)
Glaucoma, Open-Angle , Glaucoma , Trabeculectomy , Male , Humans , Retrospective Studies , Treatment Outcome , Glaucoma, Open-Angle/complications , Glaucoma, Open-Angle/surgery , Follow-Up Studies , Glaucoma/etiology , Glaucoma/surgery , Trabeculectomy/methods , Intraocular Pressure
4.
PLoS One ; 17(9): e0275180, 2022.
Article in English | MEDLINE | ID: mdl-36156600

ABSTRACT

Hereditary transthyretin amyloidosis is an autosomal dominant form of amyloidosis caused by an abnormality in transthyretin, with various ocular manifestations. Among these, ocular amyloid angiopathy has attracted attention because of its direct link to visual impairment and its correlation with systemic severity. We hypothesized that optical coherence tomography angiographic parameters would be useful biomarkers of amyloidosis systemic severity and investigated their correlation with the systemic severity score. The primary outcome was the correlation between the systemic severity score and choriocapillaris flow deficit percentage. Secondary outcomes were the correlations between the systemic severity score and retinal optical coherence tomography angiographic parameters, including foveal avascular zone size and circularity and superficial/deep/total retinal perfusion and vessel densities. The choroidal and retinal vasculature was quantified in 36 eyes from 36 patients (age, 51.8±12.1 years; disease duration, 13.4±6.2 years). Ten eyes had a history of vitrectomy for vitreous opacity. Choriocapillaris flow deficit percentage was not significantly correlated with the systemic severity score (Spearman's rank correlation: r = 2.96×10-2, p = 0.863). Similarly, foveal avascular zone size and circularity, and superficial/deep/total retinal perfusion and vessel densities were not significantly correlated with the systemic severity score. These results may indicate that optical coherence tomography angiographic parameters are not sufficient to predict amyloidosis severity.


Subject(s)
Amyloid Neuropathies, Familial , Tomography, Optical Coherence , Adult , Amyloid Neuropathies, Familial/diagnostic imaging , Fluorescein Angiography/methods , Humans , Middle Aged , Prealbumin , Retinal Vessels/diagnostic imaging , Tomography, Optical Coherence/methods
6.
Retina ; 42(1): 210-215, 2022 01 01.
Article in English | MEDLINE | ID: mdl-34483312

ABSTRACT

PURPOSE: To investigate ocular angiographic features of hereditary transthyretin amyloidosis with transthyretin Val30Met mutation (hATTR-V30M) in Japanese patients. METHODS: We retrospectively reviewed 102 eyes of 51 patients with hATTR-V30M who underwent fluorescein angiograms and indocyanine green angiograms between 2012 and 2018. Systemic severity score, fluorescein angiograms, indocyanine green angiograms, and ocular amyloidosis presentations at the final angiograms and subsequent neovascular events were evaluated. Primary outcomes were the frequency of choroidal amyloid angiopathy and retinal amyloid angiopathy (RAA). Secondary outcomes were their correlations to the systemic severity score. RESULTS: Six eyes could not be evaluated by fluorescein angiogram because of vitreous opacity. Of 96 eyes evaluated, RAA was detected in 36 (37.5%). Neovascularization was not detected. Indocyanine green angiogram indicated choroidal amyloid angiopathy in 46/51 patients (90.2%), with distinct patterns-diffuse (n = 6), focal (n = 14), and punctiform (n = 26)-based on late-phase hypercyanescence. Retinal amyloid angiopathy and choroidal amyloid angiopathy grades were associated with systemic severity (ρ = 0.57 and 0.50, respectively; both P < 0.05). At 35.4 ± 28.4 (0-96) months, iris-rubeosis was observed in one eye and vitreous hemorrhage in two. CONCLUSION: Retinal amyloid angiopathy was less common and choroidal amyloid angiopathy was frequent, and their severity correlated with the systemic severity score. The frequencies of RAA and subsequent neovascular events in this study may suggest regional differences in the ocular angiographic features of hATTR-V30M.


Subject(s)
Amyloid Neuropathies, Familial/diagnosis , Choroid/blood supply , Fluorescein Angiography/methods , Retinal Diseases/diagnosis , Retinal Vessels/diagnostic imaging , Amyloid Neuropathies, Familial/complications , Amyloid Neuropathies, Familial/genetics , Choroid/diagnostic imaging , Female , Follow-Up Studies , Fundus Oculi , Humans , Incidence , Japan/epidemiology , Male , Middle Aged , Retinal Diseases/epidemiology , Retinal Diseases/etiology , Retrospective Studies
7.
Ophthalmol Retina ; 5(10): 1005-1008, 2021 10.
Article in English | MEDLINE | ID: mdl-33422693

ABSTRACT

PURPOSE: To investigate the tomographic features in patients with hereditary amyloidosis transthyretin (hATTR). DESIGN: Retrospective case series and analysis of B-scan OCT images. PARTICIPANTS: A total of 120 patients (240 eyes) diagnosed with hATTR. METHODS: We performed a retrospective review of the treatment history and retrospective analysis of the OCT images of patients with hATTR. The parameters analyzed were the age at which the last OCT was performed, presence of ocular amyloidosis, history of pars plana vitrectomy (PPV), systemic treatment, and genetic mutations. Two independent evaluators evaluated the OCT images for characteristic needle-shaped pattern deposits on the retinal surface, and a third evaluator resolved any differences in their evaluations. MAIN OUTCOME MEASURES: The frequency of characteristic needle-shaped deposits on the retinal surface seen on OCT. RESULTS: The mean age at the time of the last OCT was 56.5 ± 14.9 years. Ninety-nine patients had gene encoding transthyretin (TTR) with the Val30Met mutation, and 21 patients had other mutations. Of 240 eyes, 128 had signs of ocular amyloidosis. Fifty of 73 eyes (68.5%) with a history of PPV for vitreous opacities exhibited characteristic deposits on OCT. Four of 31 eyes with vitreous opacity but without a history of PPV showed deposits on the retinal surface. No eyes without a history of vitreous opacities revealed the characteristic needle-shaped deposits. CONCLUSIONS: Characteristic needle-shaped deposits on the retinal surface seen on OCT are significant because they are seen in most of the vitrectomized eyes presenting with ocular amyloidosis.


Subject(s)
Amyloid Neuropathies, Familial/diagnostic imaging , Retinal Diseases/diagnostic imaging , Tomography, Optical Coherence , Adult , Aged , Amyloid Neuropathies, Familial/genetics , Amyloid Neuropathies, Familial/surgery , Female , Fluorescein Angiography , Humans , Male , Middle Aged , Prealbumin/genetics , Retinal Diseases/genetics , Retinal Diseases/surgery , Retrospective Studies , Visual Acuity/physiology , Vitrectomy , Vitreous Body/pathology , Vitreous Body/surgery
8.
Jpn J Ophthalmol ; 64(5): 533-538, 2020 Sep.
Article in English | MEDLINE | ID: mdl-32627105

ABSTRACT

PURPOSE: To investigate outcomes associated with Baerveldt glaucoma drainage implant (BGI) surgery for refractory glaucoma secondary to transthyretin (TTR)-related familial amyloid polyneuropathy (FAP) with TTR Val30Met mutation. STUDY DESIGN: Retrospective case series. METHODS: Medical records of 5 eyes of 4 patients were reviewed. All patients had refractory glaucoma secondary to FAP with a history of unsuccessful intraocular pressure (IOP) control by trabeculectomy with mitomycin C, underwent BGI surgery, and were followed up for at least 1 year. The primary outcome was mean postoperative IOP, and secondary outcomes included the number of ocular hypotensive medications and postoperative complications. RESULTS: The mean postoperative follow-up period was 52.4 months. The mean preoperative IOP of 37.0 mmHg was reduced to 13.4 mmHg immediately postoperatively, and respective subsequent means were 15.8, 13.0, 14.4, and 16.8 mmHg at 1, 3, 6, 12, and 24 months (P < 0.05). The preoperative mean number of ocular hypotensive medications of 5.4 was reduced to respective means of 2.2, 1.6, 2.8, 2.8, and 2.8 at 1, 3, 6, 12, and 24 months. One eye suffered from IOP elevation and underwent cyclophotocoagulation at 26 months. Another was dropped from further analyses because of deterioration in the patient's general condition due to FAP progression. The remaining 3 eyes exhibited adequate IOP control (13, 13, 13 mmHg) at the final visit. CONCLUSION: BGI surgery may be the current optimal treatment for patients with refractory glaucoma secondary to TTR-FAP with regard to achieving long-term IOP reduction.


Subject(s)
Amyloid Neuropathies, Familial , Glaucoma Drainage Implants , Glaucoma , Follow-Up Studies , Glaucoma/surgery , Humans , Intraocular Pressure , Postoperative Complications , Prealbumin , Prosthesis Implantation , Retrospective Studies , Treatment Outcome , Visual Acuity
9.
Sci Rep ; 10(1): 5574, 2020 03 27.
Article in English | MEDLINE | ID: mdl-32221479

ABSTRACT

We conducted a retrospective observational study including 31 eyes of 20 patients in order to investigate the efficacy of 25-gauge vitrectomy for vitreous opacity with minimal conjunctival invasion and subsequent management of intraocular pressure (IOP) secondary to hereditary transthyretin amyloidosis. We followed up these patients for an average of 44.7 ± 32.6 months. The primary outcome was best corrected visual acuity (BCVA) at 1 month after surgery and at the final follow-up visit, with management of subsequent IOP elevation. Secondary outcomes included the post-vitrectomy IOP survival rate, to determine the frequency of IOP elevation requiring glaucoma surgery. Mean age at vitrectomy was 55.4 ± 9.1 years. Logarithm of the Minimum Angle of Resolution (LogMAR) BCVA showed immediate improvement from 0.73 ± 0.62 to 0.00 ± 0.22 at 1 month (p = 4.1 × 10-7), an improvement that was maintained up to the final follow-up visit, when IOP was maintained at 13.1 ± 5.2 mmHg. The survival rate of post-vitrectomy IOP control was 0.51, 0.38, and 0.23 at 12, 24, and 60 months, respectively. A poor post-vitrectomy IOP survival rate suggests that removing vitreous amyloid via 25-gauge vitrectomy is not sufficient to guarantee good visual function; subsequent careful follow-up and proper glaucoma management is also required in order to achieve this goal.


Subject(s)
Amyloid Neuropathies, Familial/surgery , Glaucoma/surgery , Eye/physiopathology , Female , Humans , Intraocular Pressure/physiology , Male , Middle Aged , Retrospective Studies , Tonometry, Ocular/methods , Visual Acuity/physiology , Vitrectomy/methods
10.
Amyloid ; 24(1): 17-23, 2017 Mar.
Article in English | MEDLINE | ID: mdl-28081655

ABSTRACT

In order to elucidate the pathomechanism of ocular amyloid formation in a liver-transplanted patient with hereditary ATTR amyloidosis, we investigated detailed biochemical features of ocular amyloid. The patient was a 49-year-old woman with V30M transthyretin (TTR) variant (p.TTRV50M), who underwent ophthalmectomy due to corneal rupture 10 years after liver transplantation (LT). The amyloid was selectively isolated from several portions in intra- and extraocular tissues using a laser microdissection (LMD) system and analyzed by liquid chromatography-tandem mass spectrometry to determine the composition percentage of wild-type and variant TTR in the isolated amyloid. Biochemical analysis revealed that the amyloid consisted mainly of variant TTR in intraocular tissues with a percentage > 80%. On the contrary in the extraocular muscles, wild-type TTR was the main component of the amyloid with a percentage of ∼70%. Our data indicate that intraocular amyloid formation strongly depends on locally synthesized variant TTR and the contribution of wild-type TTR to amyloid formation is quite limited.


Subject(s)
Amyloid/metabolism , Amyloidosis, Familial/therapy , Liver Transplantation , Chromatography, Liquid , Female , Humans , Laser Capture Microdissection , Middle Aged , Prealbumin/metabolism , Tandem Mass Spectrometry
11.
Asia Pac J Ophthalmol (Phila) ; 3(1): 36-40, 2014.
Article in English | MEDLINE | ID: mdl-26107306

ABSTRACT

PURPOSE: Recently, an increasing number of ophthalmologists are using vitrectomy as the first line of treatment for retinal detachment (RD). The purpose of the present study was to determine the cutoff time of duration of macular detachment (DMD) after which postoperative best corrected visual acuity (BCVA) decreases sharply in eyes treated with primary vitrectomy. DESIGN: This was a retrospective, noncomparative, interventional case series. METHODS: Fifty-six eyes with macula-on RD and 126 eyes with bullous macula-off RD. RESULTS: Mean postoperative BCVA showed a statistically significant decrease when DMD exceeded 10 days (P = 0.009) with vitrectomy/phacovitrectomy as the primary mode of treatment, which was comparable to previous studies using scleral buckling. Approximately 90% (88%-93%) of eyes achieved a postoperative 20/40 BCVA when DMD was 2 days or less, after which the ratio decreased (P = 0.008) but plateaued around 40% until DMD reached 10 days. On the other hand, only 5.6% of (7/126) eyes with DMD of 3 days or less achieved a postoperative 20/20 BCVA. CONCLUSIONS: As with scleral buckling, the mean postoperative BCVA in eyes with bullous macula-off RD treated with primary vitrectomy/phacovitrectomy dropped significantly when DMD exceeded 10 days. To achieve a good postoperative visual acuity (20/40), urgent surgery with a DMD of 2 days or less is desired. Operative repair within this period, rather than outright emergency surgery, may be appropriate for most cases.

12.
Am J Med Genet A ; 152A(6): 1333-46, 2010 Jun.
Article in English | MEDLINE | ID: mdl-20503305

ABSTRACT

We previously described two unrelated patients showing characteristic facial and skeletal features, overlapping with the kyphoscoliosis type Ehlers-Danlos syndrome (EDS) but without lysyl hydroxylase deficiency [Kosho et al. (2005) Am J Med Genet Part A 138A:282-287]. After observations of them over time and encounter with four additional unrelated patients, we have concluded that they represent a new clinically recognizable type of EDS with distinct craniofacial characteristics, multiple congenital contractures, progressive joint and skin laxity, and multisystem fragility-related manifestations. The patients exhibited strikingly similar features according to their age: craniofacial, large fontanelle, hypertelorism, short and downslanting palpebral fissures, blue sclerae, short nose with hypoplastic columella, low-set and rotated ears, high palate, long philtrum, thin vermilion of the upper lip, small mouth, and micro-retrognathia in infancy; slender and asymmetric face with protruding jaw from adolescence; skeletal, congenital contractures of fingers, wrists, and hips, and talipes equinovarus with anomalous insertions of flexor muscles; progressive joint laxity with recurrent dislocations; slender and/or cylindrical fingers and progressive talipes valgus and cavum or planus, with diaphyseal narrowing of phalanges, metacarpals, and metatarsals; pectus deformities; scoliosis or kyphoscoliosis with decreased physiological curvatures of thoracic spines and tall vertebrae; cutaneous, progressive hyperextensibility, bruisability, and fragility with atrophic scars; fine palmar creases in childhood to acrogeria-like prominent wrinkles in adulthood, recurrent subcutaneous infections with fistula formation; cardiovascular, cardiac valve abnormalities, recurrent large subcutaneous hematomas from childhood; gastrointestinal, constipation, diverticula perforation; respiratory, (hemo)pneumothorax; and ophthalmological, strabismus, glaucoma, refractive errors.


Subject(s)
Abnormalities, Multiple/diagnosis , Contracture/diagnosis , Craniofacial Abnormalities/diagnosis , Ehlers-Danlos Syndrome/diagnosis , Joints/abnormalities , Abnormalities, Multiple/classification , Abnormalities, Multiple/genetics , Adolescent , Adult , Child, Preschool , Contracture/classification , Contracture/genetics , Craniofacial Abnormalities/classification , Craniofacial Abnormalities/genetics , Ehlers-Danlos Syndrome/classification , Ehlers-Danlos Syndrome/genetics , Female , Humans , Japan , Male , Skin Abnormalities/classification , Skin Abnormalities/diagnosis , Skin Abnormalities/genetics , Young Adult
14.
J Glaucoma ; 16(1): 169-70, 2007 Jan.
Article in English | MEDLINE | ID: mdl-17224769

ABSTRACT

PURPOSE: Secondary glaucoma and vitreous opacity with amyloid fibrils are the 2 major ocular complications of familial amyloid polyneuropathy (FAP). We investigated the feasibility of 25-gauge vitrectomy in excising the opaque vitreous into eyes that had already undergone trabeculectomy. METHODS: For the purpose of vision recovery, 25-gauge vitrectomy was performed in 2 eyes with vitreous amyloidosis. Both eyes had undergone trabeculectomy to treat glaucoma secondary to FAP. The survival of the filtration bleb after 25-gauge vitrectomy was also evaluated. RESULTS: Vision improved dramatically on the next day after the 25-gauge vitrectomy that causes minimal damage to the eye. No apparent complications including failure of the filtration bleb have been observed throughout the follow-up period of 6 months. CONCLUSIONS: 25-gauge vitrectomy has a potential to become a therapy of choice to excise opaque vitreous with amyloid fibrils in FAP, especially in the glaucomatous eyes that have already undergone trabeculectomy.


Subject(s)
Amyloid Neuropathies, Familial/surgery , Eye Diseases/surgery , Vitrectomy/methods , Vitreous Body/surgery , Amyloid/metabolism , Amyloid Neuropathies, Familial/complications , Amyloid Neuropathies, Familial/metabolism , Eye Diseases/etiology , Eye Diseases/metabolism , Female , Glaucoma/etiology , Glaucoma/surgery , Humans , Middle Aged , Trabeculectomy , Vitreous Body/metabolism , Vitreous Body/pathology
15.
Exp Eye Res ; 80(3): 401-12, 2005 Mar.
Article in English | MEDLINE | ID: mdl-15721622

ABSTRACT

The purposes of this study are to determine the genes that are up- or down-regulated in eyes with endotoxin-induced uveitis (EIU) by an oligonucleotide microarray system, and to determine the temporal and spatial changes in expression of selected genes that show strong up-regulation. EIU was induced by a footpad injection of lipopolysaccharide (LPS) in male Lewis rats. The expression of genes in the iris-ciliary body (ICB) at 2, 6, 12, and 24 hr after LPS injection was determined by oligonucleotide microarray analyses and compared to that in control rats. The microarray displayed 9911 genes and expressed sequence tags (ESTs). Cluster analysis was performed for highly up-regulated genes. Selected genes for cytokines (interleukin (IL)-1 beta and IL-6), chemokines (RANTES), and immediate early genes (Jun B, c-Fos, and c-Jun) were also studied by real-time polymerase chain reaction (PCR). Immunohistochemical studies were performed to localize the protein expression of some immediate early gene products. After LPS injection, the expression of 1930 genes were increased or decreased over 2-folds compared with normal controls by 24 hr. One hundred and seventeen genes were up-regulated over 10-fold, and these were classified into five clusters with similar expression pattern. The immediate early genes and transcription factors genes were included in one cluster of up-regulated genes peaking at 2 hr after the LPS injection. The expressions of cytokines, chemokines, and adhesion molecules were highly up-regulated. Real-time PCR analyses for selected genes showed similar expression changes as detected by the microarray analyses. Jun B immunoreactivity was found in the ICB cells at 3 and 6 hr after LPS injection. Gene expression changes after LPS injection were profiled by using an oligonucleotide microarray system. Our data suggest that the immediate early genes, such as Jun B, play an important role in inducing the inflammatory-related genes in the ICB.


Subject(s)
Ciliary Body/physiology , Gene Expression Regulation/genetics , Iris/physiology , Oligonucleotide Array Sequence Analysis/methods , Uveitis/genetics , Animals , Aqueous Humor/chemistry , Aqueous Humor/cytology , Cluster Analysis , Down-Regulation/genetics , Eye Proteins/analysis , Immunohistochemistry/methods , Lipopolysaccharides , Male , RNA, Messenger/analysis , Rats , Rats, Inbred Lew , Time Factors , Up-Regulation/genetics
16.
Invest Ophthalmol Vis Sci ; 44(10): 4347-56, 2003 Oct.
Article in English | MEDLINE | ID: mdl-14507879

ABSTRACT

PURPOSE: To systematically explore changes in gene expression in the retina of monkeys with laser-induced glaucoma and to validate the microarray data on eyes with experimental glaucoma. METHODS: Glaucoma was induced in the right eye of four monkeys by repeated argon laser photocoagulation of the trabecular meshwork. The left eye served as the control. Retinas were isolated from glaucomatous and control eyes 30 days after photocoagulation. Gene expression changes were analyzed by human microarray chips which displayed a total of 9182 elements including Expression Sequence Tag (EST) clones. Changes in the expression of some genes were further confirmed by real-time PCR analysis. Immunohistochemical studies to examine protein expression of some gene products were also done for several genes that showed up- or downregulation by the microarray analysis. RESULTS: Two eyes with mild glaucoma and two with severe glaucoma were produced. In the mild and severe glaucomatous retina, the number of upregulated genes was 45 and 18, and the number of downregulated genes was 17 and 21, respectively. The number of genes that were up- or downregulated was 0.7% of all the genes examined. The real-time PCR analysis confirmed expression changes of some genes found in the microarray analysis. Ceruloplasmin was one of the upregulated genes, and it was found by immunohistochemical analyses to be expressed in Müller cells. CONCLUSIONS: Gene expression profiles in laser-induced glaucomatous monkey retinas were determined, and only a very small population of genes was up- or downregulated in glaucomatous eyes. Upregulation of ceruloplasmin protein was found in the Müller cells.


Subject(s)
Eye Proteins/genetics , Gene Expression Profiling , Glaucoma/genetics , Oligonucleotide Array Sequence Analysis , Retina/metabolism , Animals , Ceruloplasmin/metabolism , Disease Models, Animal , Fluorescent Antibody Technique, Indirect , Glaucoma/metabolism , Glial Fibrillary Acidic Protein/metabolism , Intraocular Pressure , Laser Coagulation , Macaca fascicularis , Reverse Transcriptase Polymerase Chain Reaction , Trabecular Meshwork/surgery , Up-Regulation
17.
Am J Ophthalmol ; 136(2): 361-4, 2003 Aug.
Article in English | MEDLINE | ID: mdl-12888066

ABSTRACT

PURPOSE: To evaluate changes in the retinal nerve fiber layer following traumatic optic neuropathy. DESIGN: Observational case report. METHODS: A patient presented with visual loss after an accident. Scanning laser polarimetry was performed. RESULTS: Thickness of the retinal nerve fiber layer increased immediately after the trauma but then progressively decreased. Severe loss was observed at day 90 and then ceased. Enlargement of the optic disk cup was also observed. CONCLUSIONS: This is the first report documenting early transient increase followed by progressive loss of the retinal nerve fiber layer in traumatic optic neuropathy.


Subject(s)
Diagnostic Techniques, Ophthalmological , Nerve Fibers/pathology , Optic Nerve Injuries/diagnosis , Optic Nerve/pathology , Retinal Ganglion Cells/pathology , Adolescent , Bicycling/injuries , Glucocorticoids/therapeutic use , Humans , Lasers , Male , Methylprednisolone/therapeutic use , Nerve Fibers/drug effects , Optic Nerve/drug effects , Optic Nerve Injuries/drug therapy , Optic Nerve Injuries/physiopathology , Retinal Ganglion Cells/drug effects , Vision Disorders/diagnosis , Vision Disorders/drug therapy , Vision Disorders/physiopathology , Visual Fields
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