Pectin-Chitosan Hydrogel Beads for Delivery of Functional Food Ingredients.

A common challenge in hydrogel-based delivery systems is the premature release of low molecular weight encapsulates through diffusion or swelling and reduced cell viability caused by the low pH in gastric conditions. A second biopolymer, such as chitosan, can be incorporated to overcome this. Chitosan is usually associated with colonic drug delivery systems. We intended to formulate chitosan-coated pectin beads for use in delaying premature release of the encapsulate under gastric conditions but allowing release through disintegration under intestinal conditions. The latter is of utmost importance in delivering most functional food ingredients. Therefore, this study investigated the impact of formulation and process conditions on the size, sphericity, and dissolution behavior of chitosan-coated hydrogel beads prepared by interfacial coacervation. The size and sphericity of the beads depend on the formulation and range from approximately 3 to 5 mm and 0.82 to 0.95, respectively. Process conditions during electro-dripping may be modulated to tailor bead size. Depending on the voltage, bead size ranged from 1.5 to 4 mm. Confocal laser scanning microscopy and scanning electron microscopy confirmed chitosan shell formation around the pectin bead. Chitosan-coated beads maintained their size and shape in simulated gastric fluid but experienced structural damage in simulated intestinal fluid. Therefore, they represent a novel delivery system for functional food ingredients.

A new genus of Herpyllobiidae (Copepoda: Cyclopoida) from a deep-living annelid (Polychaeta: Sigalionidae).

A taxonomic study of deep-sea polychaetes collected at a depth of 2,805 m off the northern coast of California revealed a scaleworm of the family Sigalionidae with an attached parasitic copepod. The copepod represents an undescribed genus of the family Herpyllobiidae, comprising mesoparasitic copepods chiefly recorded from polychaetes of the family Polynoidae. Blakerius gen. nov. diverges from the other herpyllobiid genera by its possession of 1) a chalice-shaped ectosoma with several protuberances along the posterior margin and a long cylindrical shaft with a hyaline coating and integumental sculpturing, a short stalk with a small, anteriorly placed sclerotized ring, 2) a relatively large, discoid-shaped endosoma with digitiform process, and 3) attached male copepodids with 3-segmented antennules, containing limbless sac-like males. The new genus is compared with other herpyllobiids. This discovery increases the number of known herpyllobiid genera to six and is the first record of a herpyllobiid parasitizing a sigalionid polychaete.urn: lsid: zoobank.org:pub:5E31FEED-D3EB-460E-AEA4-02A9D3A778D6.

Cytotoxic Activity of Lepidium virginicum L. Methanolic Extract on Human Colorectal Cancer Cells, Caco-2, through p53-Mediated Apoptosis.

Colorectal cancer (CRC) is the third most common type of cancer worldwide. Its treatment options have had a limited impact on cancer remission prognosis. Therefore, there is an ongoing need to discover novel anti-cancer agents. Medicinal plants have gained recognition as a source of anti-cancer bioactive compounds. Recently, ethanolic extract of L. virginicum stems ameliorated dinitrobenzene sulfonic acid (DNBS)-induced colitis by modulating the intestinal immune response. However, no scientific study has demonstrated this potential cytotoxic impact on colon cancer cells. The objective of this study was to evaluate the cytotoxic effect of the methanolic extract of L. virginicum (ELv) on a human colorectal adenocarcinoma cell line (Caco-2) and to identify and quantify the phenolic compounds present in ELv extracts by liquid chromatography-mass spectrometry analysis. The cytotoxic activity was assessed using cell viability assays by reduction in the compound 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH). MTT and LDH assays revealed that the ELv decreases cell viability in the Caco-2 cell line in a concentration-dependent manner. Cell death was a result of DNA fragmentation and p53-mediated apoptosis. Eight phenolic acids and five flavonoids were identified and quantified in the stems. In conclusion, our findings demonstrate that the extract of L. virginicum possesses cytotoxic properties on Caco-2 cell line, suggesting that it could be a potential source of new drugs against CRC.

Soft-metal(loid)s induce protein aggregation in Escherichia coli.

Metal(loid) salts were used to treat infectious diseases in the past due to their exceptional biocidal properties at low concentrations. However, the mechanism of their toxicity has yet to be fully elucidated. The production of reactive oxygen species (ROS) has been linked to the toxicity of soft metal(loid)s such as Ag(I), Au(III), As(III), Cd(II), Hg(II), and Te(IV). Nevertheless, few reports have described the direct, or ROS-independent, effects of some of these soft-metal(loid)s on bacteria, including the dismantling of iron-sulfur clusters [4Fe-4S] and the accumulation of porphyrin IX. Here, we used genome-wide genetic, proteomic, and biochemical approaches under anaerobic conditions to evaluate the direct mechanisms of toxicity of these metal(loid)s in Escherichia coli. We found that certain soft-metal(loid)s promote protein aggregation in a ROS-independent manner. This aggregation occurs during translation in the presence of Ag(I), Au(III), Hg(II), or Te(IV) and post-translationally in cells exposed to Cd(II) or As(III). We determined that aggregated proteins were involved in several essential biological processes that could lead to cell death. For instance, several enzymes involved in amino acid biosynthesis were aggregated after soft-metal(loid) exposure, disrupting intracellular amino acid concentration. We also propose a possible mechanism to explain how soft-metal(loid)s act as proteotoxic agents.

Mastigodiaptomus galapagoensis n. sp. (Crustacea: Copepoda: Diaptomidae), a possibly extinct copepod from a crater lake of the Galápagos archipelago.

Background: A new species of a Neotropical diaptomid copepod is described based on individuals recovered from a small, almost forgotten collection of unique plankton samples from El Junco, a crater lake in San Cristóbal island, Galápagos archipelago. This copepod was regularly reported (1966-2004) as an abundant zooplankter in the lake, but it was not found in subsequent plankton surveys (2007-2018), and its specific identity remained unknown. In 2020, it was declared extinct because of introduced fish predation, rotenone treatment, and other major disturbances. The taxonomic examination of these invaluable specimens allowed us to recognize them as representing an undescribed species of the freshwater diaptomid genus Mastigodiaptomus Light, 1939. Methods: Here, we describe the new species from El Junco crater lake, located in the San Cristóbal island a part of the Galápagos archipelago, collected with plankton nets. The description is based on detailed morphology, based on SEM and light microscopy. Results: The taxonomic examination of these invaluable specimens allowed us to recognize them as representing an undescribed species of the freshwater diaptomid genus Mastigodiaptomus Light, 1939. The new species was readily assigned to this genus and is distinguished from its known congeners by details of (1) the male right fifth leg terminal claw and aculeus, (2) spiniform processes pattern of the right geniculate antennule segments 10-16, (3) length and structure of the spiniform process of the antepenultimate segment of the male right antennule, and (4) details of the dorsal process on the female fourth pediger. This finding represents the first report of this Neotropical copepod genus outside its original biogeographic region, the third species of a diaptomid copepod reported from insular freshwater systems, the southernmost record of Mastigodiaptomus, and the only freshwater calanoid in the Galápagos. The intriguing presence of this chiefly Neotropical copepod genus here could be related either to (1) human agency linked to pirate activities, commercial travelling by Spaniard ships, whaling activities, and intense tortoise hunting in San Cristóbal island. In the past, El Junco was the only freshwater source 600 nautical miles around, or (2) zoochory of resistant dormant stages passively transported by more than 65 migrating bird species known to settle in San Cristóbal. These two hypotheses cannot be properly tested at this time, so the explanation of the presence of this copepod will remain as a new open question in the fascinating natural history of the Galápagos.

Assessment of Dentinal Tubules Penetration by Hyben X Delivered into the Root Canal System as an Irrigant; An in Vitro Study.

Introduction: Disinfection of the root canal system is crucial for a successful endodontic treatment. Several factors influence the performance of effective irrigation. Diverse irrigating substances have been used but none has proved to completely penetrate the root canal system. HybenX dries biofilm due to its hygroscopic properties; therefore, it is effective in the treatment of biofilm-related diseases. This investigation aimed to estimate HybenX's degree of penetration into the dentinal tubules of upper first premolars. Materials and Methods: Experimental in vitro intervention where 30 extracted maxillary premolars were evaluated to determine HybenX's degree of penetration and 5% sodium hypochlorite in the dentinal tubules using three different irrigation techniques (passive ultrasonic irrigation, dynamic manual irrigation and conventional single jet irrigation technique). After preparation, the root canals were irrigated with fluorescent rhodamine 6G; then 500-micrometer sections were made to be analyzed under a fluorescence microscope. Measurements were made in micrometer in ZEN software to determine the penetration degree of each substance in the different root thirds. Results: In the apical third, significant differences between the 3 irrigation techniques were found (P<0.05), similarly, for the middle and cervical thirds. Significant statistical differences between the HybenX and sodium hypochlorite were found at the cervical and middle levels when using the conventional Monoject irrigation and passive ultrasonic irrigation techniques. As for the apical level, differences were found between the passive ultrasonic irrigation techniques and the dynamic manual irrigation technique. Conclusions: Based on this in vitro study, HybenX proved to highly penetrate into the dentinal tubules, especially when using the passive ultrasonic irrigation technique. HybenX may be a useful option for root canal irrigation in endodontics.

New records of Harpacticoids (Crustacea, Copepoda) from a coastal system of northern Colombia

Three species of harpacticoids are herein recorded from the zooplankton of Rodadero beach, Santa Marta, Magdalena, Colombia. Samples were collected from the littoral zone, mainly at inshore areas covered by mangrove vegetation and in an adjacent oyster bank. One of them, Laophonte cornuta Philippi, 1840 is new to the Colombian harpacticoid fauna, the other two species: Distioculus minor (Scott T., 1894) and Microsetella norvegica (Boeck, 1865) are new reports to the Magdalena department. This is the first illustrated record confirming their presence in Colombia and Magdalena, northern Colombia. Comparative morphological comments and illustrations of these species are also provided to document this report.

Se registran tres especies de harpacticoides en el zooplancton de playa Rodadero, Santa Marta, Magdalena, norte de Colombia. Se recolectaron muestras en la zona litoral, principalmente en áreas costeras de manglar y en un banco de ostras. Una de ellas, Laophonte cornuta Philippi, 1840 es nueva para la fauna de harpacticoides de Colombia; las otras dos especies: Distioculus minor (Scott T., 1894) y Microsetella norvegica (Boeck 1865) son nuevos reportes para el departamento del Magdalena. Este es el primer registro ilustrado que confirma su presencia en Colombia y Magdalena. Se proporcionan comentarios morfológicos comparativos e ilustraciones de estas especies.

A new Cardiodectes Wilson, 1917 (Hexanauplia: Copepoda: Siphonostomatoida) parasitic on a scarid teleost (Perciformes: Scaridae) from Roatan Island, Central America.

Members of the parasitic copepod family Pennellidae are highly transformed ecto- or mesoparasites infecting a wide array of marine teleosts. Currently, this family contains more than 20 valid genera. The pennellid genus Cardiodectes Wilson, 1917 is currently known to contain 15 nominal species. Some pennellids exhibit a complex life cycle involving an intermediate host; it is known that planktonic pteropod molluscs are intermediate hosts for Cardiodectes. Pennellid mesoparasites can be detected by the conspicuous female egg-carrying trunk on the host external surface. The copepod cephalothorax is deeply embedded in the host muscle tissue. Members of Cardiodectes have been reported from several teleost families, mainly Myctophidae and Engraulidae. From the parasitological examination of a juvenile individual of a scarid teleost collected in a reef lagoon of Roatan Island, Honduras, Central America, several ovigerous female individuals of a mesoparasitic pennellid copepod were found; these specimens were recognized as representative of an undescribed species of Cardiodectes Wilson, 1917. The new species, C. roatanensis n. sp., differs from its known congeners in several respects, including the presence of neck lobes, paired posterior protuberances of the trunk, trunk shape and proportions, structure of cephalothorax lobes, cephalothorax relative size, and number of legs. The new species from Roatan is the second member of this copepod genus to be reported from the Caribbean region, after C. boxshalli Bellwood, 1981 from off Jamaica (Bellwood 1981). It is also the second report of Cardiodectes on a parrotfish.

TNF-α enhances sensory DRG neuron excitability through modulation of P2X3 receptors in an acute colitis model.

Previous studies have demonstrated that acute colonic inflammation leads to an increase in dorsal root ganglia (DRG) neuronal excitability. However, the signaling elements implicated in this hyperexcitability have yet to be fully unraveled. Extracellular adenosine 5'-triphosphate (ATP) is a well-recognized sensory signaling molecule that enhances the nociceptive response after inflammation through activation of P2X3 receptors, which are expressed mainly by peripheral sensory neurons. The aim of this study is to continue investigating how P2X3 affects neuronal hypersensitivity in an acute colitis animal model. To achieve this, DNBS (Dinitrobenzene sulfonic acid; 200 mg/kg) was intrarectally administered to C57BL/6 mice, and inflammation severity was assessed according to the following parameters: weight loss, macroscopic and microscopic scores. Perforated patch clamp technique was used to evaluate neuronal excitability via measuring changes in rheobase and action potential firing in T8-L1 DRG neurons. A-317491, a well-established potent and selective P2X3 receptor antagonist, served to dissect their contribution to recorded responses. Protein expression of P2X3 receptors in DRG was evaluated by western blotting and immunofluorescence. Four days post-DNBS administration, colons were processed for histological analyses of ulceration, crypt morphology, goblet cell density, and immune cell infiltration. DRG neurons from DNBS-treated mice were significantly more excitable compared with controls; these changes correlated with increased P2X3 receptor expression. Furthermore, TNF-α mRNA expression was also significantly higher in inflamed colons compared to controls. Incubation of control DRG neurons with TNF-α resulted in similar cell hyperexcitability as measured in DNBS-derived neurons. The selective P2X3 receptor antagonist, A-317491, blocked the TNF-α-induced effect. These results support the hypothesis that TNF-α enhances colon-innervating DRG neuron excitability via modulation of P2X3 receptor activity.

Food-Grade Bigels with Potential to Replace Saturated and Trans Fats in Cookies.

Fats play multiple roles in determining the desirable characteristics of foods. However, there are health concerns about saturated and trans fats. Bigels have been proposed as a novel fat replacer in foods. This research evaluated the role of the type of hydrogel in the development of bigels to be used as fat replacers in cookies. Bigels were made with beeswax/canola oil oleogel and sodium alginate and carboxymethylcellulose hydrogels. The results showed that the peroxide value and binding capacity of bigels were affected by the type of hydrogel used. However, their fatty acid profile, p-anisidine value, oxidative stability, and texture remained unchanged. Using bigels as fat replacers, cookies were obtained with a hardness similar to those with original shortening, showing the potential of bigels for use in foods.

Dynamic performance-Energy tradeoff consolidation with contention-aware resource provisioning in containerized clouds.

Containers have emerged as a more portable and efficient solution than virtual machines for cloud infrastructure providing both a flexible way to build and deploy applications. The quality of service, security, performance, energy consumption, among others, are essential aspects of their deployment, management, and orchestration. Inappropriate resource allocation can lead to resource contention, entailing reduced performance, poor energy efficiency, and other potentially damaging effects. In this paper, we present a set of online job allocation strategies to optimize quality of service, energy savings, and completion time, considering contention for shared on-chip resources. We consider the job allocation as the multilevel dynamic bin-packing problem that provides a lightweight runtime solution that minimizes contention and energy consumption while maximizing utilization. The proposed strategies are based on two and three levels of scheduling policies with container selection, capacity distribution, and contention-aware allocation. The energy model considers joint execution of applications of different types on shared resources generalized by the job concentration paradigm. We provide an experimental analysis of eighty-six scheduling heuristics with scientific workloads of memory and CPU-intensive jobs. The proposed techniques outperform classical solutions in terms of quality of service, energy savings, and completion time by 21.73-43.44%, 44.06-92.11%, and 16.38-24.17%, respectively, leading to a cost-efficient resource allocation for cloud infrastructures.

A new species of Nitokra Boeck, 1865 (Copepoda, Harpacticoida, Ameiridae) from the Caribbean coast of Colombia.

Biological samples obtained from a coastal system of northern Colombia yielded male and female specimens of an undescribed harpacticoid copepod of the diverse ameirid genus Nitokra Boeck, 1865. The new species is a member of the genus group III. We describe the new species based on adult male and female individuals. Nitokrapuebloviejensis sp. nov., appears to be most closely related to N.vietnamensis Tran & Chang, 2012, but they can be separated by the following characters: 1) number of setal elements on second segment of mandibular palp, 2) P1ENP/EXP ratio, 3) relative lengths of P2, P3ENP/EXP, 4) number of elements on male P5EXP and ENP, and 5) segmentation of male antennule. In addition, N.puebloviejensis sp. nov. can be confused with two other congeners: N.taylori Gómez, Carrasco & Morales-Serna, 2012 from South Africa and Colombia and N.kastjanensis Kornev & Chertoprud, 2008 from the White Sea, but the new species can be distinguished from them by: 1) number of setae on the maxillule coxa, 2) P1ENP/EXP ratio, 3) P2,P3ENP/EXP ratio, 4) female and male P5 setophore, 5) setation pattern of female P5EXP and ENP, 6) structure of female P6, 7) ornamentation of female anal operculum, 8) number of setae on male P5EXP, and 9) the male antennule segmentation. Most importantly, the presence of a group of five short setae on the medial surface of the maxilliped syncoxa allows the new species to be readily distinguished from its congeners. Only two subspecies and one species of this genus have been hitherto recorded from Colombia. A key to the 23 known American species of Nitokra is provided.

A new species of Esola Edwards, 1891 (Crustacea, Copepoda, Harpacticoida, Laophontidae) from the Caribbean coast of Colombia.

A new species of the harpacticoid copepod genus Esola is described from specimens collected in Rodadero Beach, on Gaira Bay, on the Caribbean coast of Colombia. The species, E.wellsi sp. nov., is described, illustrated, and com-pared with its congeners. Esolawellsi sp. nov. differs from its known congeners in details of the armature of legs 1-4. It most closely resembles E.bulbifera (Norman, 1911) in the armature formula of P1-P5 but differs from the latter in several respects, including the female antennule segmentation (7-segmented in E.bulbifera but distinctly 6-segmented in E.wellsi sp. nov.) and in the shape and size of the male P3ENP2 apophysis, among other characters. This is the second species of the genus known from the Caribbean and the second record of Esola in the Northwestern Tropical Atlantic. The genus now contains eight species. A key to the known species of the genus is also included.

Hyperiids (Amphipoda, Hyperiidea) collected during the TALUD cruises in western Mexico. 5. Family Amphithyridae, with the description of a new species of Amphithyropsis Zeidler.

In order to complete the information related to the little studied deep-sea planktonic fauna of western Mexico, samples from a wide depth range (surface to 1550 m depth) were obtained using different gear. Six species and 108 individuals of hyperiid amphipods of the family Amphithyridae were collected at 26 localities, including a new species of Amphithyropsis Zeidler, 2016, which is herein described based on an adult male and a gravid female. Other species include Amphithyrus bispinosus Claus, 1879, the most abundant and frequently collected species (70 specimens at 17 localities), A. muratus Volkov, 1982 (11 specimens at 8 localities), A. sculpturatus Claus, 1879 (14 specimens at 7 localities), Paralycaea gracilis Claus, 1879 (10 specimens from 6 localities), and P. hoylei Stebbing, 1888 (one specimen from one locality). Worldwide and regional distributions are provided for each species.

Comparing Machine Learning Methods to Improve Fall Risk Detection in Elderly with Osteoporosis from Balance Data.

Falls are a multifactorial cause of injuries for older people. Subjects with osteoporosis are particularly vulnerable to falls. We study the performance of different computational methods to identify people with osteoporosis who experience a fall by analysing balance parameters. Balance parameters, from eyes open and closed posturographic studies, and prospective registration of falls were obtained from a sample of 126 community-dwelling older women with osteoporosis (age 74.3 ± 6.3) using World Health Organization Questionnaire for the study of falls during a follow-up of 2.5 years. We analyzed model performance to determine falls of every developed model and to validate the relevance of the selected parameter sets. The principal findings of this research were (1) models built using oversampling methods with either IBk (KNN) or Random Forest classifier can be considered good options for a predictive clinical test and (2) feature selection for minority class (FSMC) method selected previously unnoticed balance parameters, which implies that intelligent computing methods can extract useful information with attributes which otherwise are disregarded by experts. Finally, the results obtained suggest that Random Forest classifier using the oversampling method to balance the data independent of the set of variables used got the best overall performance in measures of sensitivity (>0.71), specificity (>0.18), positive predictive value (PPV >0.74), and negative predictive value (NPV >0.66) independent of the set of variables used. Although the IBk classifier was built with oversampling data considering information from both eyes opened and closed, using all variables got the best performance (sensitivity >0.81, specificity >0.19, PPV = 0.97, and NPV = 0.66).

First record of the freshwater copepod Mesocyclops paranaensis Dussart & Frutos, 1986 (Copepoda: Cyclopoida: Cyclopidae) from Colombia / Primer registro del copépodo de aguadulce Mesocyclops paranaensis Dussart& Frutos, 1986 (Copepoda: Cyclopoida: Cyclopidae) de Colombia

Abstract The Neotropical free-living freshwater cyclopoid copepod Mesocyclops paranaensis Dussart & Frutos, 1986 was found in a small temporal pond in La Guajira, northern Colombia. Hitherto, it has been reported from Argentina, Paraguay, and Brazil. This is the first record of this species in Colombia, its northernmost finding in South America, and the fourth locality in which this species has been recorded from. Mesocyclops paranaensis can be distinguished from its closest congeners by a unique combination of the characters of the female, including: 1) leg 4 intercoxal sclerite with two large, acute projections; 2) P3, P4 intercoxal sclerite caudal surface lightly pilose; 3) seminal receptacle with narrow lateral arms and weakly convex anterior margin; 4) second antennary endopodite with seven setae, and insertion of antennary exopodal seta with adjacent spinules; 5)frontal surface of P1 basipodite ornamented with long spinules; 6) posterior margin of anal somite with continuous row of spinules; and 7) inner margin of caudal ramus hirsute. The Colombian population shows some subtle morphological differences with respect to previous reports.

Resumen El copépodo ciclopoide de agua dulce Mesocyclops paranaensis Dussart & Frutos, 1986 fue encontrado en un pequeño estanque temporal al norte de La Guajira. Hasta ahora, esta especie había sido registrada en Paraguay, Argentina y Brasil. Este es el primer registro de esta especie sudamericana en Colombia, su registro más septentrional en América del Sur, y la cuarta localidad en la que se registra. Mesocyclops paranaensis se puede distinguir de sus congéneres más cercanos por una combinación única de caracteres que incluyen: 1) esclerito intercoxal de la pata 4 con dos grandes proyecciones puntiagudas, 2) esclerito intercoxal de la pata 3 y pata 4 ligeramente piloso, 3) receptáculo seminal con brazos laterales estrechos y margen anterior débilmente convexo, 4)segundo segmento endopodal de la antena con siete setas, inserción de la seta exopodal de la antena con espínulas, 5) superficie frontal del basipodito de la pata 1 ornamentada con largas espínulas, 6) posterior margen del somita anal de la hembra con hileras continuas de espínulas y 7) margen interno de la rama caudal pilosa. Se proporciona una breve descripción de esta especie e ilustraciones de la población colombiana y se compara con su congénere más cercano. Los especímenes de Colombia muestran algunas diferencias morfológicas con respecto a los reportes previos acerca de esta especie.

A new Bradophila Marchenkov, 2002 (Copepoda, Cyclopoida, Bradophilidae) parasitic on a flabelligerid polychaete from the Chukchi Sea.

The cyclopoid copepod family Bradophilidae includes a few species of mesoparasitic copepods infecting flabelligerid polychaetes. It contains two species of Bradophila Levinsen, 1878, the type genus: B. pygmaea Levinsen, 1878 and B. minuta Boxshall, O'Reilly, Sikorski & Summerfield, 2019, both known from North Europe. Two other genera (i.e., Trophoniphila M'Intosh, 1885 and Flabellicola Gravier, 1918) have some affinities with this family including their host preference. Mesoparasitic copepods are highly specialized, morphologically reduced forms. Part of their body (endosoma) is partially lodged in the host body and the other part is external (ectosoma); both parts are connected by an intersomital stalk. Infection by these copepods can be readily detected by the presence of the egg-carrying ectosoma on the host external surface. From the analysis of flabelligerid polychaetes collected in 2012 from the Chukchi Sea, two ovigerous female individuals of a bradophilid copepod were recorded. These specimens were recognized as representative of an undescribed species of Bradophila. The new species, B. susanae n. sp., shows the generic diagnostic characters and differs from its two other known congeners in several respects, including the cuticular ectosomal ornamentation, body proportions, size of the intersomital stalk, position of the genital pore, and shape and arrangement of egg sacs. Also, the new species ectosomal size range (0.440 - 0.450 µm) falls between the size range of its two known congeners. Our finding expands the known host range of bradophilid copepods to include a new flabelligerid host, Bradabyssa nuda (Annenkova-Chlopina) from the Russsian Arctic region.

Effect of Interfacial Ionic Layers on the Food-Grade O/W Emulsion Physical Stability and Astaxanthin Retention during Spray-Drying.

The utilization of astaxanthin in food processing is considered to be narrow because of its substandard solubility in aqueous matrices and the instability of chemical compounds during the processing of food and the instability of chemical compounds during the processing of food. The investigation sought to evaluate multilayer emulsions stabilized by ionic interfacial layers of lupin protein isolate (LPI), ι-carrageenan (CA), and chitosan (CHI) on the physical stability of the emulsion as well as the retention of astaxanthin during the spray drying process. Primary emulsion (Pr-E) was prepared by adding LPI on oil droplet surfaces containing astaxanthin. The homogenization pressure and cycles to obtain the Pr-E were investigated. The secondary emulsion (Se-E) and tertiary emulsion (Te-E) were elaborated by mixing CA/Pr-E and CHI/Se-E, respectively. Emulsion stability was assessed under different environmental stresses (pH and NaCl). Astaxanthin retention of emulsions was determined immediately after finishing the spray-drying process. The results showed that Pr-E was stabilized with 1.0% (w/v) of LPI at 50 MPa and three cycles. Se-E and Te-E were obtained with CA/Pr-E and Se-E/CHI of 70/30 and 50/50% (w/w), respectively. The Se-E was the most stable compared to the Pr-E and Te-E when subjected to different pHs; nevertheless, once the NaCl concentration rose, no variations in the ζ-potential of all emulsions studied or destabilization were observed. The Se-E and Te-E derived provided higher astaxanthin retention (>95%) during the spray-drying process compared to Pr-E (around 88%). The results indicated that these astaxanthin multilayer emulsions show considerable potential as a functional ingredient in food products.

Activation of regulator ArcA in the presence of hypochlorite in Salmonella enterica serovar Typhimurium.

Oxidative stress is the main mechanism behind efficient disinfectants, causing damage in bacterial macromolecules. Importantly, bacteria activate resistance mechanisms in response to damage generated by oxidative stress. Strategies allowing pathogens to survive oxidative stress are highly conserved among microorganisms. Many of these strategies entail genomic responses triggered by signals transduced through Two Component Systems (TCS). Recently, we demonstrated that the TCS ArcAB (specifically ArcA) participates in bacterial responses to hypochlorite, regulating the uptake of this toxic compound and being involved in resistance and survival inside neutrophils, where hypochlorous acid abounds. Here, we demonstrated that ArcA is required in the response to oxidative stress generated by hypochlorite, independent of its cognate sensor ArcB or the Asp54 of ArcA, the only phosphorylable residue in ArcA, which is required to function as a gene regulator. Our results suggest that ArcA could have additional functions to respond to oxidative stress, independent of its regulatory activity, which might require interaction with other unknown relevant proteins.

Validation of Cymbasoma turcorum and Monstrillopsis pontoeuxinensis, two monstrilloids from Turkey described by Suárez-Morales amp; Üstün in 2018 (Crustacea: Copepoda: Monstrilloida).

Recently, Suárez-Morales Üstün (2018) described two new species of monstrilloid copepods, Cymbasoma turcorum and Monstrillopsis pontoeuxinensis from Turkish coastal waters of the Black Sea. The morphological descriptions, illustrations, and type designations presented in that paper fully characterized both new species; however, the journal issue in which the description appeared was published only online, with no print version (Suárez-Morales Üstün 2018), and the article in which these new names were introduced did not include a ZooBank registration number (LSID) for the article or any other evidence of such registration. This is currently required by Article 8.5 of the International Code of Zoological Nomenclature for an electronic work to be deemed "published" under the Code (International Commission on Zoological Nomenclature 2012). Inasmuch as Suárez-Morales Üstün (2018) is an "unpublished work", the specific names turcorum and pontoeuxinensis proposed in it are unavailable. The present note is intended to fully validate these two names by proposing them once again as new while also fulfilling all of the Code's current conditions for nomenclatural availability of this work itself and the two new names. The date and authorship of these two specific names will, accordingly, be those of this note, not Suárez-Morales Üstün (2018).