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1.
J Clin Exp Dent ; 16(7): e873-e878, 2024 Jul.
Article in English | MEDLINE | ID: mdl-39219831

ABSTRACT

Background: Noncarious cervical lesions (NCCLs) is the dental structure loss unrelated to caries. The aim was to investigate the possible relationship between bruxism, age, gender, daily routine and dietary habits and NCCL, and correlate with sensitivity and position in the dental arch. Material and Methods: 245 patients aged between 18 and 40 participated in the survey, in which a questionnaire focused on parafunctional habits, erosion, abrasion, dental abfraction and acidic diet was conducted. Facial symmetry analysis, masseter hypertrophy, occlusion evaluation, and presence of wear facets and NCCL. Data were expressed as absolute and percentage frequency and were analyzed using the Fisher's Exact/Chi-square tests and a multinomial logistic regression model. Results: NCCLs was observed in 46.6 % in the participants. Bruxism was observed in 64% of the individuals, and stress in 33%. Wear facets and acidic diets were reported in 75% and 60% of the sample, respectively. There was no significant relationship between NCCL and gender (p = 0.74), bruxism (p = 0.33), stress (p = 0.52), wear facets (p = 0.73), and acidic diets (p = 0.39). Age over 30 years was more strongly associated with NCCL (p< 0.001). Conclusions: Age showed a direct correlation with noncarious cervical lesions. Factors including gender and dietary and parafunctional habits, such as bruxism, were not differential regarding the presence of NCCL. Key words:Tooth wear, non-carious cervical lesions, bruxism.

2.
Access Microbiol ; 6(9)2024.
Article in English | MEDLINE | ID: mdl-39239567

ABSTRACT

Auxins, mainly in the form of indole-3-acetic acid (IAA), regulate several aspects of plant and algal growth and development. Consequently, plant and algae-associated bacteria developed the ability to modulate IAA levels, including IAA catabolism. In this work, we present and analyse the genome sequence of the IAA-degrading and marine algae-associated bacterium, Marinomonas sp. NFXS50, analyse its IAA catabolism gene cluster and study the prevalence of IAA catabolism genes in other Marinomonas genomes. Our findings revealed the presence of homologs of the Pseudomonas iac gene cluster, implicated in IAA catabolism, in the genome of strain NFXS50; however, differences were observed in the content and organization of the Marinomonas iac gene cluster when compared to that of the model iac-containing Pseudomonas putida 1290. These variations suggest potential adaptations in the IAA catabolism pathway, possibly influenced by substrate availability and evolutionary factors. The prevalence of iac genes across several Marinomonas species underscores the significance of IAA catabolism in marine environments, potentially influencing plant/algae-bacteria interactions. This study provides novel insights into the IAA catabolism in Marinomonas, laying the groundwork for future investigations into the role of iac genes in Marinomonas physiology and the regulation of marine plant/algae-bacteria interactions.

3.
Access Microbiol ; 6(9)2024.
Article in English | MEDLINE | ID: mdl-39346683

ABSTRACT

We describe the whole-genome sequences of seven diverse marine bacteria isolated from Portuguese environments that presented the ability to promote the growth of the model diatom, Phaeodactylum tricornutum. The bacterial genome sequences will contribute to the study of genetic and molecular mechanisms involved in diatom-bacteria interactions.

4.
Access Microbiol ; 6(7)2024.
Article in English | MEDLINE | ID: mdl-39130742

ABSTRACT

The study presents the whole genome sequence of the carotenoid-producing Paracoccus sp. NFXS7, isolated from a marine saltern in Setúbal, Portugal. The carotenoid-producing strain NFXS7 contains homologs of the crt genes involved in astaxanthin biosynthesis, making it a promising candidate for biotechnological applications.

5.
Sci Total Environ ; 946: 174263, 2024 Oct 10.
Article in English | MEDLINE | ID: mdl-38936733

ABSTRACT

Sediments polluted with hydrophobic organic contaminants (HOCs) and metals can pose environmental risks, yet effective remediation remains a challenge. We investigated a new composite sorbent comprising granular activated carbon (GAC) and a calcium-silicate (Polonite®, PO) for thin-layer capping of polluted sediment, with the aim to sequester both HOCs and metals. Box cores were collected in polluted Oskarshamn harbor, Sweden, and the sediments were treated with GAC and/or Polonite in a 10-week mesocosm study to measure endpoints ranging from contaminant immobilization to ecological side effects on native fauna and biogeochemical processes. The GAC particle size was 300-500 µm to reduce negative effects on benthic fauna (by being non-ingestible) and of biogenic origin (coconut) to have a small carbon footprint compared with traditional fossil ACs. The calcium-silicate was a fine-grained industrial by-product used to target metals and as a carrier for GAC to improve the cap integrity. GAC decreased the uptake of dioxins (PCDD/Fs) in the bivalve Macoma balthica by 47 % and the in vitro bioavailability of PCB by 40 %. The composite cap of GAC + Polonite decreased sediment-to-water release of Pb < Cu < Ni < Zn < Cd by 42-98 % (lowest to highest decrease) and bioaccumulation of Cd < Zn < Cu in the worm Hediste diversicolor by 50-65 %. Additionally, in vitro bioavailability of Pb < Cu < Zn, measured using digestive fluid extraction, decreased by 43-83 %. GAC showed no adverse effects on benthic fauna while Polonite caused short-term adverse effects on fauna diversity and abundance, partly due to its cohesiveness, which, in turn, can improve the cap integrity in situ. Fauna later recovered and bioturbated the cap. Both sorbents influenced biogeochemical processes; GAC sorbed ammonium, Polonite decreased respiration, and both sorbents reduced denitrification. In conclusion, the side effects were relatively mild, and the cap decreased the release and bioavailability of both HOCs and metals effectively, thus offering a promising sustainable and cost-effective solution to remediating polluted sediments.


Subject(s)
Calcium Compounds , Charcoal , Environmental Restoration and Remediation , Geologic Sediments , Silicates , Water Pollutants, Chemical , Geologic Sediments/chemistry , Charcoal/chemistry , Environmental Restoration and Remediation/methods , Silicates/chemistry , Sweden , Calcium Compounds/chemistry , Animals , Metals
6.
Sci Total Environ ; 945: 174183, 2024 Oct 01.
Article in English | MEDLINE | ID: mdl-38909808

ABSTRACT

Coastal areas are an important source of methane (CH4). However, the exact origins of CH4 in the surface waters of coastal regions, which in turn drive sea-air emissions, remain uncertain. To gain a comprehensive understanding of the current and future climate change feedbacks, it is crucial to identify these CH4 sources and processes that regulate its formation and oxidation. This study investigated coastal CH4 dynamics by comparing water column data from six stations located in the brackish Tvärminne Archipelago, Baltic Sea. The sediment biogeochemistry and microbiology were further investigated at two stations (i.e., nearshore and offshore). These stations differed in terms of stratification, bottom water redox conditions, and organic matter loading. At the nearshore station, CH4 diffusion from the sediment into the water column was negligible, because nearly all CH4 was oxidized within the upper sediment column before reaching the sediment surface. On the other hand, at the offshore station, there was significant benthic diffusion of CH4, albeit the majority underwent oxidation before reaching the sediment-water interface, due to shoaling of the sulfate methane transition zone (SMTZ). The potential contribution of CH4 production in the water column was evaluated and was found to be negligible. After examining the isotopic signatures of δ13C-CH4 across the sediment and water column, it became apparent that the surface water δ13C-CH4 values observed in areas with thermal stratification could not be explained by diffusion, advective fluxes, nor production in the water column. In fact, these values bore a remarkable resemblance to those detected below the SMTZ. This supports the hypothesis that the source of CH4 in surface waters is more likely to originate from ebullition than diffusion in stratified brackish coastal systems.

7.
Food Chem ; 455: 139879, 2024 Oct 15.
Article in English | MEDLINE | ID: mdl-38824725

ABSTRACT

We used confocal microscopy and spectrofluorescence to characterize the emission spectra in hop flowers, to follow the isomerization processes in different hop preparations, and beers, to compare with HPLC extracted samples. Flowers of different hop cultivars produced in three regions of Brazil, were quantitated by HPLC and GC-MS. The fluorescence spectra showed two characteristic emission bands evaluated from different preparations. The isomerization process leads to a gradual decrease in fluorescence intensity as the reaction progresses. This demonstrates the valuable use of confocal microscopy and fluorescence spectroscopy for analysis of the correlation between bitter acid indices with fluorescence intensity and lifetime microscopy. Such techniques can be used directly in the flowers allowing rapid monitoring of the brewing process. Twenty-nine substances were characterized in the essential oils and some cultivars presented quantities of bitter acids and essential oil levels close to those expected for plants after more than three years of cultivation.


Subject(s)
Beer , Flowers , Humulus , Microscopy, Confocal , Oils, Volatile , Brazil , Flowers/chemistry , Flowers/metabolism , Humulus/chemistry , Chromatography, High Pressure Liquid , Beer/analysis , Oils, Volatile/chemistry , Isomerism , Spectrometry, Fluorescence/methods , Gas Chromatography-Mass Spectrometry
8.
ISME J ; 18(1)2024 Jan 08.
Article in English | MEDLINE | ID: mdl-38366020

ABSTRACT

Coastal ecosystems dominate oceanic methane (CH4) emissions. However, there is limited knowledge about how biotic interactions between infauna and aerobic methanotrophs (i.e. CH4 oxidizing bacteria) drive the spatial-temporal dynamics of these emissions. Here, we investigated the role of meio- and macrofauna in mediating CH4 sediment-water fluxes and aerobic methanotrophic activity that can oxidize significant portions of CH4. We show that macrofauna increases CH4 fluxes by enhancing vertical solute transport through bioturbation, but this effect is somewhat offset by high meiofauna abundance. The increase in CH4 flux reduces CH4 pore-water availability, resulting in lower abundance and activity of aerobic methanotrophs, an effect that counterbalances the potential stimulation of these bacteria by higher oxygen flux to the sediment via bioturbation. These findings indicate that a larger than previously thought portion of CH4 emissions from coastal ecosystems is due to faunal activity and multiple complex interactions with methanotrophs.


Subject(s)
Ecosystem , Methane , Bacteria/genetics , Water
9.
Sci Total Environ ; 921: 171076, 2024 Apr 15.
Article in English | MEDLINE | ID: mdl-38382611

ABSTRACT

Soft sediment marine benthic ecosystems comprise a diverse community of bacteria, meiofauna and macrofauna, which together support a range of ecosystem processes such as biogeochemical cycling. These ecosystems are also fishing grounds for demersal species that are often caught using bottom trawling. This fishing method can have deleterious effects on benthic communities by causing injury or mortality, and through alteration of sediment properties that in turn influence community structure. Although the impacts of bottom trawling on macrofauna are relatively well studied, less is known about the responses of meiofauna and bacteria to such disturbances, or how bottom trawling impacts benthic ecosystem processes. Quantifying trawling impacts against a background of natural environmental variability is also a challenge. To address these questions, we examined effects of bottom trawling and a range of environmental variables (e.g. water chemistry and physical and biochemical surface sediment properties) on a) bacterial, meiofaunal and macrofaunal community structure and b) benthic ecosystem processes (nutrient fluxes, extracellular enzyme activities and carbon turnover and degradation rates). We also investigated the link between the benthic macrofauna community and the same ecosystem processes. While there was a significant effect of bottom trawling intensity on macrofaunal community structure, the same was not seen for bacterial or meiofaunal community composition, which were more affected by environmental factors, such as surface sediment properties. The labile component of the surface sediment carbon pool was higher at highly trawled sites. Carbon degradation rates, extracellular enzyme activities, oxygen fluxes and some nutrient fluxes were significantly affected by trawling, but ecosystem processes were also strongly linked to the abundance of key bioturbators (Macoma balthica, Halicryptus spinulosus, Scoloplos armiger and Pontoporeia femorata). Although benthic ecosystems were affected by a combination of trawling and natural variability, disentangling these showed that the anthropogenic effects were clearest on the larger component of the community, i.e. macrofauna composition, and on ecosystem processes related to sedimentary carbon.


Subject(s)
Bivalvia , Ecosystem , Animals , Biodiversity , Bacteria , Carbon , Geologic Sediments/chemistry
10.
Rev. bras. entomol ; Rev. bras. entomol;68(3): e20240025, 2024. graf
Article in English | LILACS-Express | LILACS | ID: biblio-1569702

ABSTRACT

ABSTRACT A fortuitous observation of mate-guarding behavior of male Chrysoprasis a. auriventris is presented here. In our observation, a male hold onto the female's body using his forelegs and walks along with her while she lays the eggs into the trunk slits. While guarding the female, the male adopted aggressive postures and attacked rival males that approached her. We suggest that the mate-guarding behavior may explain the male's elongated mid and hindlegs in this species, under a functional perspective. Additionally, a new host plant association was recorded.

11.
Int. braz. j. urol ; 49(5): 580-589, Sep.-Oct. 2023. tab, graf
Article in English | LILACS-Express | LILACS | ID: biblio-1506417

ABSTRACT

ABSTRACT Objective: To report outcomes from the largest multicenter series of penile cancer patients undergoing video endoscopic inguinal lymphadenectomy (VEIL). Materials and Methods: Retrospective multicenter analysis. Authors of 21 centers from the Penile Cancer Collaborative Coalition-Latin America (PeC-LA) were included. All centers performed the procedure following the same previously described standardized technique. Inclusion criteria included penile cancer patients with no palpable lymph nodes and intermediate/high-risk disease and those with non-fixed palpable lymph nodes less than 4 cm in diameter. Categorical variables are shown as percentages and frequencies whereas continuous variables as mean and range. Results: From 2006 to 2020, 210 VEIL procedures were performed in 105 patients. Mean age was 58 (45-68) years old. Mean operative time was 90 minutes (60-120). Mean lymph node yield was 10 nodes (6-16). Complication rate was 15.7%, including severe complications in 1.9% of procedures. Lymphatic and skin complications were noted in 8.6 and 4.8% of patients, respectively. Histopathological analysis revealed lymph node involvement in 26.7% of patients with non-palpable nodes. Inguinal recurrence was observed in 2.8% of patients. 10y- overall survival was 74.2% and 10-y cancer specific survival was 84.8%. CSS for pN0, pN1, pN2 and pN3 were 100%, 82.4%, 72.7% and 9.1%, respectively. Conclusion: VEIL seems to offer appropriate long term oncological control with minimal morbidity. In the absence of non-invasive stratification measures such as dynamic sentinel node biopsy, VEIL emerged as the alternative for the management of non-bulky lymph nodes in penile cancer.

12.
Rev. Ciênc. Saúde ; 13(2): 42-51, Junho 2023.
Article in English, Portuguese | LILACS | ID: biblio-1444167

ABSTRACT

Objetivo: Identificar a importância da comunicação para o cuidado seguro e efetivo de enfermagem em centro cirúrgico e terapia intensiva.Métodos: Revisão integrativa, com levantamento das produções em fevereiro de 2023 nas bases de dados Lilacs, MEDLINE, CINAHL e Scopus. Foram incluídos estudos primários, disponíveis na íntegra, em inglês, português e espanhol.Resultados: Das 1.904 produções inicialmente encontradas, 19 foram incluídos para análise. Os estudos apontaram que a comunicação é fundamental para a garantia da segurança do paciente e inserção da família no processo do cuidado. Essa habilidade vai além da expressão verbal, envolvendo outras formas de se comunicar. Contudo, necessita ser melhorada em algumas situações, evitando principalmente a ocorrência de danos ao cliente. Sugere-se ainda que algumas formas de comunicação requerem ser padronizadas.Conclusão: A comunicação é uma das ações prioritárias e fundamentais para a segurança e efetividade do cuidado de enfermagem, seja nos centros cirúrgicos ou nas unidades de terapia intensiva. Diante desse contexto, se faz necessário que as barreiras de comunicação sejam identificadas e que ações estratégicas organizacionais sejam colocadas em prática, entre elas a educação permanente dos profissionais, bem como a padronização de informações. Ações como essas minimizam os potenciais riscos que permeiam os cuidados críticos e tornam positivas as relações no ambiente de trabalho


Objective: To identify the importance of communication for safe and effective nursing care in the surgical center and intensive care. Methods: An integrative review with a survey of productions in February 2023 in the databases Lilacs, MEDLINE, CINAHL, and Scopus. Primary studies, available in full in English, Portuguese, and Spanish, were included. Results: Of the 1,904 productions initially found, 19 were included for analysis. The studies pointed out that communication is essential to guaranty patient safety and to insert the family in the care process. This skill goes beyond verbal expression and involves other ways of communicating.However, it needs to be improved in some situations, mainly to avoid damage to the customer. It is also suggested that some forms of communication require to be standardized. Conclusion: Communication is one of the priorities and fundamental actions for the safety and effectiveness of nursing care, whether in surgical centers or intensive care units. Given this context, it is necessary that communication barriers are identified and that strategic organizational actions are put into practice, including the permanent education of professionals and the standardization of information. Actions like these minimize the potential risks that permeate critical care and positively impact relationships in the work environment


Subject(s)
Humans , Male , Female , Adult , Patients , Work , Critical Care , Education
13.
Int Braz J Urol ; 49(5): 580-589, 2023.
Article in English | MEDLINE | ID: mdl-37390124

ABSTRACT

OBJECTIVE: To report outcomes from the largest multicenter series of penile cancer patients undergoing video endoscopic inguinal lymphadenectomy (VEIL). MATERIALS AND METHODS: Retrospective multicenter analysis. Authors of 21 centers from the Penile Cancer Collaborative Coalition-Latin America (PeC-LA) were included. All centers performed the procedure following the same previously described standardized technique. Inclusion criteria included penile cancer patients with no palpable lymph nodes and intermediate/high-risk disease and those with non-fixed palpable lymph nodes less than 4 cm in diameter. Categorical variables are shown as percentages and frequencies whereas continuous variables as mean and range. RESULTS: From 2006 to 2020, 210 VEIL procedures were performed in 105 patients. Mean age was 58 (45-68) years old. Mean operative time was 90 minutes (60-120). Mean lymph node yield was 10 nodes (6-16). Complication rate was 15.7%, including severe complications in 1.9% of procedures. Lymphatic and skin complications were noted in 8.6 and 4.8% of patients, respectively. Histopathological analysis revealed lymph node involvement in 26.7% of patients with non-palpable nodes. Inguinal recurrence was observed in 2.8% of patients. 10y- overall survival was 74.2% and 10-y cancer specific survival was 84.8%. CSS for pN0, pN1, pN2 and pN3 were 100%, 82.4%, 72.7% and 9.1%, respectively. CONCLUSION: VEIL seems to offer appropriate long term oncological control with minimal morbidity. In the absence of non-invasive stratification measures such as dynamic sentinel node biopsy, VEIL emerged as the alternative for the management of non-bulky lymph nodes in penile cancer.


Subject(s)
Penile Neoplasms , Video-Assisted Surgery , Aged , Humans , Male , Middle Aged , Inguinal Canal/surgery , Inguinal Canal/pathology , Lymph Node Excision/methods , Lymph Nodes/pathology , Penile Neoplasms/surgery , Penile Neoplasms/pathology , Treatment Outcome , Video-Assisted Surgery/methods , Retrospective Studies
14.
Microb Ecol ; 86(4): 2357-2372, 2023 Nov.
Article in English | MEDLINE | ID: mdl-37222807

ABSTRACT

Denitrification in sediments is a key microbial process that removes excess fixed nitrogen, while dissimilatory nitrate reduction to ammonium (DNRA) converts nitrate to ammonium. Although microorganisms are responsible for essential nitrogen (N) cycling, it is not yet fully understood how these microbially mediated processes respond to toxic hydrophobic organic compounds (HOCs) and metals. In this study, we sampled long-term polluted sediment from the outer harbor of Oskarshamn (Baltic Sea), measured denitrification and DNRA rates, and analyzed taxonomic structure and N-cycling genes of microbial communities using metagenomics. Results showed that denitrification and DNRA rates were within the range of a national reference site and other unpolluted sites in the Baltic Sea, indicating that long-term pollution did not significantly affect these processes. Furthermore, our results indicate an adaptation to metal pollution by the N-cycling microbial community. These findings suggest that denitrification and DNRA rates are affected more by eutrophication and organic enrichment than by historic pollution of metals and organic contaminants.


Subject(s)
Ammonium Compounds , Microbiota , Nitrates , Denitrification , Nitrogen , Oxidation-Reduction
15.
Microorganisms ; 11(4)2023 Mar 30.
Article in English | MEDLINE | ID: mdl-37110324

ABSTRACT

Marine bacteria are a significant source of bioactive compounds for various biotechnological applications. Among these, actinomycetes have been found to produce a wide range of secondary metabolites of interest. Saccharopolyspora is one of the genera of actinomycetes that has been recognized as a potential source of these compounds. This study reports the characterization and genomic analysis of Saccharopolyspora sp. NFXS83, a marine bacterium isolated from seawater from the Sado estuary in Portugal. The NFXS83 strain produced multiple functional and stable extracellular enzymes under high-salt conditions, showed the ability to synthesize auxins such as indole-3-acetic acid, and produced diffusible secondary metabolites capable of inhibiting the growth of Staphylococcus aureus. Furthermore, when Phaeodactylum tricornutum was co-cultivated with strain NFXS83 a significant increase in microalgae cell count, cell size, auto-fluorescence, and fucoxanthin content was observed. Detailed analysis revealed the presence of clusters involved in the production of various secondary metabolites, including extracellular enzymes, antimicrobial compounds, terpenes, and carotenoids in the genome of strain NFXS83. Ultimately, these findings indicate that Saccharopolyspora sp. NFXS83 has a significant potential for a wide range of marine biotechnological applications.

16.
ISME Commun ; 3(1): 25, 2023 Mar 27.
Article in English | MEDLINE | ID: mdl-36973336

ABSTRACT

While theories and models have appeared to explain genome size as a result of evolutionary processes, little work has shown that genome sizes carry ecological signatures. Our work delves into the ecological implications of microbial genome size variation in benthic and pelagic habitats across environmental gradients of the brackish Baltic Sea. While depth is significantly associated with genome size in benthic and pelagic brackish metagenomes, salinity is only correlated to genome size in benthic metagenomes. Overall, we confirm that prokaryotic genome sizes in Baltic sediments (3.47 Mbp) are significantly bigger than in the water column (2.96 Mbp). While benthic genomes have a higher number of functions than pelagic genomes, the smallest genomes coded for a higher number of module steps per Mbp for most of the functions irrespective of their environment. Some examples of this functions are amino acid metabolism and central carbohydrate metabolism. However, we observed that nitrogen metabolism was almost absent in pelagic genomes and was mostly present in benthic genomes. Finally, we also show that Bacteria inhabiting Baltic sediments and water column not only differ in taxonomy, but also in their metabolic potential, such as the Wood-Ljungdahl pathway or the presence of different hydrogenases. Our work shows how microbial genome size is linked to abiotic factors in the environment, metabolic potential and taxonomic identity of Bacteria and Archaea within aquatic ecosystems.

17.
Plants (Basel) ; 12(3)2023 Feb 01.
Article in English | MEDLINE | ID: mdl-36771735

ABSTRACT

Microalgae are important members of the soil and plant microbiomes, playing key roles in the maintenance of soil and plant health as well as in the promotion of plant growth. However, not much is understood regarding the potential of different microalgae strains in augmenting plant growth, or the mechanisms involved in such activities. In this work, the functional and genomic characterization of strain NFX-FRZ, a eukaryotic microalga belonging to the Micractinium genus that was isolated from the rhizosphere of a plant growing in a natural environment in Portugal, is presented and analyzed. The results obtained demonstrate that strain NFX-FRZ (i) belongs to a novel species, termed Micractinium rhizosphaerae sp. nov.; (ii) can effectively bind to tomato plant tissues and promote its growth; (iii) can synthesize a wide range of plant growth-promoting compounds, including phytohormones such as indole-3-acetic acid, salicylic acid, jasmonic acid and abscisic acid; and (iv) contains multiple genes involved in phytohormone biosynthesis and signaling. This study provides new insights regarding the relevance of eukaryotic microalgae as plant growth-promoting agents and helps to build a foundation for future studies regarding the origin and evolution of phytohormone biosynthesis and signaling, as well as other plant colonization and plant growth-promoting mechanisms in soil/plant-associated Micractinium.

18.
Foods ; 12(1)2023 Jan 03.
Article in English | MEDLINE | ID: mdl-36613429

ABSTRACT

The bacterial species Gluconacetobacter entanii belongs to a group of acetic acid bacteria. In 2000, it was described as a primary species of submerged spirit vinegar-producing bioreactors with a strict requirement of acetic acid, ethanol, and glucose for growth. Over the years, the type-strain of G. entanii deposited in international culture collections has lost the ability for revitalization and is thus not available any more in a culturable form. Here, we have systematically characterized phenotypic features and genomes of recently isolated G. entanii strains and compared them with characteristics of the type-strain available from published data. Using the functional annotation, genes gmhB and psp were identified as unique for G. entanii genomes among species in the clade Novacetimonas. The genome stability of G. entanii was assessed after 28 and 43 months of preculturing the strain Gluconacetobacter entanii AV429 twice a week. The strain G. entanii AV429 did not accumulate giant insertions or deletions but a few gene mutations. To unify further research into acetic acid bacteria systematics and taxonomy, we propose G. entanii AV429 as the neotype strain.

19.
Nat Commun ; 14(1): 42, 2023 01 03.
Article in English | MEDLINE | ID: mdl-36596795

ABSTRACT

Coastal ecosystems can efficiently remove carbon dioxide (CO2) from the atmosphere and are thus promoted for nature-based climate change mitigation. Natural methane (CH4) emissions from these ecosystems may counterbalance atmospheric CO2 uptake. Still, knowledge of mechanisms sustaining such CH4 emissions and their contribution to net radiative forcing remains scarce for globally prevalent macroalgae, mixed vegetation, and surrounding depositional sediment habitats. Here we show that these habitats emit CH4 in the range of 0.1 - 2.9 mg CH4 m-2 d-1 to the atmosphere, revealing in situ CH4 emissions from macroalgae that were sustained by divergent methanogenic archaea in anoxic microsites. Over an annual cycle, CO2-equivalent CH4 emissions offset 28 and 35% of the carbon sink capacity attributed to atmospheric CO2 uptake in the macroalgae and mixed vegetation habitats, respectively, and augment net CO2 release of unvegetated sediments by 57%. Accounting for CH4 alongside CO2 sea-air fluxes and identifying the mechanisms controlling these emissions is crucial to constrain the potential of coastal ecosystems as net atmospheric carbon sinks and develop informed climate mitigation strategies.


Subject(s)
Carbon Dioxide , Ecosystem , Methane , Climate Change , Carbon Sequestration , Wetlands
20.
Foods ; 12(23)2023 Dec 01.
Article in English | MEDLINE | ID: mdl-38231871

ABSTRACT

This study aimed to subject the albino peach palm to cooking and drying processes and characterize the raw pulp (RP), cooked pulp (CP), raw pulp flour (RPF), and cooked pulp flour (CPF). The product's chemical composition, bioactive compounds, and physicochemical, color, thermal, morphological, and functional-technological properties were evaluated. The proximate composition showed that carbohydrates were the main constituents of all the products (69.59-72.08 g/100 g). The cooking process decreased the lipids (10.21 to 8.63 g/100 g), dietary fiber (13.64 to 12.81 g/100 g), and total sugar content (59.18 to 49.10 g/100 g) of the CP. The colorimetric parameters indicated a significant browning of the CP and CPF, which can be attributed to the Maillard reaction and lipid oxidation. After cooking, the total phenolic compound and ascorbic acid content decreased in the pulp. The RPF and CPF displayed different thermogravimetric behaviors. The spectral patterns in the infrared region showed the characteristic bands of organic compounds that are present in the structure of starches. The scanning electron microscopy showed amyloplast and fiber bundles with starches in the RP and gelatinized starch granules in the CP and CPF. The RPF presented small and heterogeneous starch granules with isolated amyloplast. The RPF and CPF showed different granulometric patterns and technological indices. The results suggest that the pulp and flour from the pulp of albino peach palms can be exploited by the food, pharmaceutical, and biotechnological industries.

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