ABSTRACT
A total of 227 marine isolates of ubiquitous fungi were cultivated on different media and the secondary metabolite content of the extracts (ethyl acetate/chloroform/methanol 3:2:1) characterized by HPLC. The fungi were secured from animals, plants and sediments of Venezuelan waters (0-10 m) including mangroves and lagoonal areas. The extracts were tested for antibacterial activity. A total of 7 were active towards Vibrio parahaemolyticus and 55 towards Staphylococcus aureus, representing 18 different fungal species from 8 ascomycetous genera. For 61 strains of Penicillium citrinum antibacterial activity correlated well with content of secondary metabolites as measured by HPLC. Thirteen isolates of Penicillium steckii produced very similar profiles of secondary metabolites and 6 of these had activity against either V. parahaemolyticus or S. aureus or both.
Subject(s)
Anti-Bacterial Agents/pharmacology , Fungi/metabolism , Geologic Sediments/microbiology , Seawater/microbiology , Staphylococcus aureus/drug effects , Vibrio parahaemolyticus/drug effects , Anti-Bacterial Agents/analysis , Anti-Bacterial Agents/metabolism , Chromatography, High Pressure Liquid , Fungi/isolation & purification , Microbial Sensitivity Tests , Penicillium/isolation & purification , Penicillium/metabolism , Venezuela , Water MicrobiologyABSTRACT
Two new flavones were isolated from the medicinal plant Calliandra californica, 7,2',4',5'-tetramethoxyflavone [1] and 5-hydroxy-7,2',4',5'-tetramethoxyflavone [2]. The structures were determined from spectral evidence and both compounds were synthesized. Compound 2 exhibited antimicrobial activity for two test bacterial strains.
Subject(s)
Anti-Infective Agents/isolation & purification , Flavonoids/isolation & purification , Plants, Medicinal/chemistry , Anti-Bacterial Agents , Anti-Infective Agents/pharmacology , Bacillus subtilis/drug effects , California , Candida albicans/drug effects , Flavonoids/pharmacology , Microbial Sensitivity Tests , Staphylococcus aureus/drug effectsABSTRACT
The medicinal plant Lepechinia hastata, used as a remedy against uterine infections in Baja California Sur (Mexico), was shown to contain carnosol as the main diterpenoid secondary metabolite. Carnosol has potent in vitro antimicrobial activity. Detailed spectroscopical properties of carnosol are presented.