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Western blot assay using recombinant p26 antigen for detection of equine infectious anemia virus-specific antibodies.

Alvarez, I; Gutierrez, G; Ostlund, E; Barrandeguy, M; Trono, K.

Clin Vaccine Immunol ; 14(12): 1646-8, 2007 Dec.

Article in English | MEDLINE | ID: mdl-17959820

ABSTRACT

We analyzed the performance of a single-band Western blot (WB) test using recombinant p26 (rp26) capsid protein of equine infectious anemia virus. According to the results obtained, the rp26 WB test is a reliable confirmatory diagnostic tool to be used as a complementary test after an enzyme-linked immunosorbent assay or agar gel immunodiffusion test yielding doubtful results.

Subject(s)

Antibodies, Viral/analysis , Blotting, Western/veterinary , Infectious Anemia Virus, Equine/isolation & purification , Viral Core Proteins/chemistry , Animals , Blotting, Western/methods , Blotting, Western/standards , Enzyme-Linked Immunosorbent Assay/veterinary , Escherichia coli/genetics , Horses , Immunodiffusion/methods , Immunodiffusion/standards , Immunodiffusion/veterinary , Infectious Anemia Virus, Equine/genetics , Infectious Anemia Virus, Equine/immunology , Recombinant Proteins/chemistry , Recombinant Proteins/immunology , Reproducibility of Results , Serologic Tests/veterinary , Viral Core Proteins/genetics , Viral Core Proteins/immunology

ABSTRACT

Subject(s)

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