Improving the representation of stream water sources in surrogate nutrient models with water isotope data.

An important part of meeting nutrient reduction goals in the lower Great Lakes basin and assessing the success of different land management strategies is modeling nutrient losses from agricultural land. This study aimed to improve the representation of water source contributions to streamflow in generalized additive models for predicting nutrient fluxes from three headwater agricultural streams in southern Ontario monitored during the Multi-Watershed Nutrient Study (MWNS). The previous development of these models represented baseflow contributions to streamflow using the baseflow proportion derived using an uncalibrated recursive digital filter. Recursive digital filters are commonly used to partition stream discharge into separate components from slower and faster pathways. In this study, we calibrated the recursive digital filter using stream water source information from stable isotopes of oxygen in water. Across sites, optimization of the filter parameters reduced bias in baseflow estimates by as much as 68 %. In most cases, calibrating the filter also improved agreement between filter-derived baseflow and baseflow calculated from isotope and streamflow data: the average Kling-Gupta Efficiencies using default and calibrated parameters were 0.44 and 0.82, respectively. When incorporated into the generalized additive models, the revised baseflow proportion predictor was more often statistically significant, improved model parsimony, and reduced prediction uncertainty. Moreover, this information allowed for a more rigorous interpretation of how different stream water sources influence nutrient losses from the agricultural MWNS watersheds.

Total and dissolved phosphorus losses from agricultural headwater streams during extreme runoff events.

Eutrophication continues to be a concerning global water quality issue. Managing and mitigating harmful algal blooms demands clear information on the conditions promoting large phosphorus losses from contributing watersheds. Of particular concern is the amount and form of phosphorus loading to receiving water bodies during extreme runoff events, which are expected to increase in frequency due to climate change. Five years (2015 to 2020) of water quantity and quality data from 11 agricultural watersheds in the lower Great Lakes basin were analyzed and used to model total and dissolved phosphorus losses. This study aimed to assess temporal dynamics in phosphorus concentrations and losses over runoff events covering a wide range of hydrologic conditions and to quantify their relative importance on annual phosphorus losses. Event concentration-discharge relationships for total and dissolved phosphorus were hysteretic and had contrasting dominant patterns across watersheds. The proportion of annual phosphorus losses during events was highly variable between watersheds, accounting for 47-94 %. Extreme events were particularly impactful: as few as three events per year were found to be responsible for nearly half of total phosphorus (20-50 %) and total dissolved phosphorus (14-44 %) losses. Variability in total and dissolved phosphorus losses and concentrations over a wide range of flow conditions suggests that event magnitude is an important control on the relative mobility of particulate and dissolved phosphorus fractions. This study showed that insights into nutrient dynamics and phosphorus budgets in the lower Great Lakes basin and agriculture dominated environments more broadly can be gained by assessing event nutrient losses with respect to flow conditions and patterns in concentration-discharge relationships.

Novel predictors related to hysteresis and baseflow improve predictions of watershed nutrient loads: An example from Ontario's lower Great Lakes basin.

Eutrophication has re-emerged in the lower Great Lakes basin resulting in critical water quality issues. Models that accurately predict nutrient loading from streams are needed to inform appropriate nutrient management decisions. Generalized additive models (GAMs) that use surrogate data from sensors to predict nutrient loads offer an alternative to commonly applied linear regression and may better handle relationship non-linearities and skewed water quality data. Five years (2015-2020) of water quantity and quality data from 11 agricultural watersheds in southern Ontario were used to develop GAMs to predict total phosphorus (TP) and nitrate (NO3-) loads. This study aimed to 1) use GAMs to predict nutrient loads using both common and novel predictors and 2) quantify and examine the variability in seasonal and annual nutrient loads. Along with routine surrogate model predictors (i.e., flow, turbidity, and seasonality), the addition of the baseflow proportion and the hydrograph position of flow observations improved model performance. Conversely, including the antecedent precipitation index minimally affected model performance, regardless of constituent. Seasonal and annual patterns in TP and NO3- load predictions mirrored that of the hydrologic regime. This study showed that parsimonious GAMs featuring novel model predictors can be used to predict nutrient loads while accounting for the partitioning of surface and subsurface flow paths and hysteresis between streamflow and water quality parameters that are frequently observed in a wide range of environments.

Increases in salinity following a shift in hydrologic regime in a constructed wetland watershed in a post-mining oil sands landscape.

Bitumen extraction via surface mining in the Athabasca Oil Sands Region results in permanent alteration of boreal forests and wetlands. As part of their legal requirements, oil companies must reclaim disturbed landscapes into functioning ecosystems. Despite considerable work establishing upland forests, only two pilot wetland-peatland systems integrated within a watershed have been constructed to date. Peatland reclamation is challenging as it requires complete reconstruction with few guidelines or previous work in this region. Furthermore, the variable sub-humid climate and salinity of tailings materials present additional challenges. In 2012, Syncrude Canada Ltd. constructed a 52-ha pilot upland-wetland system, the Sandhill Fen Watershed, which was designed with a pump and underdrain system to provide freshwater and enhance drainage to limit salinization from underlying soft tailings materials that have elevated electrical conductivity (EC) and Na+. The objective of this research is to evaluate the hydrochemical response of a constructed wetland to variations in hydrology and water management with respect to water sources, flow pathways and major chemical transformations in the three years following commissioning. Results suggest that active water management practices in 2013 kept EC relatively low, with most wetland sites <1000 µS/cm with Na+ concentrations <250 mg/L. With limited management in 2014 and 2015, the EC increased in the wetland to >1000 µS/cm in 2014 and >2000 µS/cm in 2015. The most notable change was the emergence of several Na+ enriched zones in the margins. Here, Na+ concentrations were two to three times higher than other sites. Stable isotopes of water support that the Na+ enriched areas arise from underlying process-affected water in the tailings, providing evidence of its upward transport and seepage under a natural hydrologic regime. In future years, salinity is expected to evolve in its flow pathways and diffusion, yet the timeline and extent of these changes are uncertain.

Entorhinal cortex lesions disrupt the transition between the use of intra- and extramaze cues for navigation in the water maze.

This study with rats examined the effects of excitotoxic lesions to the entorhinal cortex (EC) and hippocampus (HPC) on using extramaze and intramaze cues to navigate to a hidden platform in a water maze. HPC lesions resulted in a disruption to the use of extramaze cues, but not intramaze cues, whereas EC lesions had no effect on the use of these cues when they were encountered for the first time. However, prior navigation training in which 1 type of cue was relevant disrupted navigation with the other type in rats with EC lesions. Results show that the EC contributes to the processing of spatial information, but that this contribution is most apparent when there is a conflict between 2 sources of navigational cues in the water maze.

The influence of selective lesions to components of the hippocampal system on the orienting [correction of orientating] response, habituation and latent inhibition.

The contribution that components of the hippocampal system in the rat make to the modulation of attention or stimulus processing was assessed using several simple behavioural assays: the orienting response (OR) to a novel stimulus, the subsequent habituation and dishabituation of this OR, and the latent inhibition effect that typically results from repeated exposure to a stimulus. Excitotoxic lesions of components of the hippocampal system produce dissociable effects on the OR, habituation and latent inhibition: lesions of the entorhinal cortex have no effect on the OR or changes in the OR during exposure to a stimulus, but disrupt latent inhibition; lesions of the subiculum disrupt the OR but not latent inhibition; and lesions of the hippocampus disrupt the OR and latent inhibition. These effects have important implications for our understanding of habituation and latent inhibition, and the neural mechanisms involved in attentional modulation.

Selective cytotoxic lesions of the retrohippocampal region produce a mild deficit in social recognition memory.

Although a number of studies have implicated the hippocampal formation in social recognition memory in the rat, a recent study in this laboratory has demonstrated that selective cytotoxic lesions, confined to the hippocampus proper (encompassing the four CA subfields and the dentate gyrus), are without effect on this behaviour. This finding suggests that the hippocampus proper does not subserve social recognition memory in the rat, but does not preclude the possibility that other areas of the hippocampal formation, such as the entorhinal cortex or subiculum, could support this form of learning. The present study addressed this issue by examining the effects of selective cytotoxic retrohippocampal (RHR) lesions (including both the entorhinal cortex and subiculum) on social recognition memory in the rat. RHR lesions produced a mild social recognition memory impairment, although lesioned animals still displayed a reduction in investigation time between the first and second exposure to the juvenile. This result is consistent with other studies which have implicated the retrohippocampal or parahippocampal area in olfactory recognition memory processes. It also suggests, however, that other areas, out with the retrohippocampal region, are also likely to play an important role in social recognition memory.

Involvement of the entorhinal cortex in a process of attentional modulation: evidence from a novel variant of an IDS/EDS procedure.

Novel behavioral assays were used to assess the role of the entorhinal cortex in modulating attention to components of stimulus compounds. In Stage 1, rats received discrimination training with compounds constructed from 3 dimensions (auditory, visual, and tactile); in each compound the combination of components from 2 dimensions (e.g., auditory and visual) were relevant to the solution of the discrimination, and the remaining dimension (e.g., tactile) was irrelevant. In Stage 2, rats received a different discrimination in which the relevant dimensions were either congruent (auditory and visual) or incongruent (auditory and tactile) with those that were relevant in Stage 1. Sham-operated rats acquired the congruent discrimination more rapidly than the incongruent discrimination--a finding indicative of a process of attentional modulation--whereas rats with excitotoxic lesions of the entorhinal cortex acquired both discriminations equally readily.

Excitotoxic lesions of the hippocampus leave sensory preconditioning intact: implications for models of hippocampal function.

Learning about contextual cues is markedly disrupted in rats with hippocampal lesions. One analysis of this disruption is that it reflects a general failure to form associations between the elements of complex events. A straightforward prediction of this analysis is that sensory preconditioning will be disrupted by hippocampal lesions. This prediction was assessed by presenting rats with flavored solutions composed of 2 elements (A and X) before X was paired with an injection of the emetic, lithium chloride. A subsequent test revealed that rats were less willing to consume Solution A than they were to consume a control solution, B. This was true of rats with sham lesions and those with excitotoxic lesions of hippocampus. These findings fail to support the proposition that the hippocampus-dependent deficit in contextual conditioning is due to a general disruption to the process of associating the elements of complex events.

Disruption of comprehension by the meaning of irrelevant sound.

This study investigates the claim that the disruption of comprehension by irrelevant sound is qualitatively different from that of short-term memory for order. Both meaningful and meaningless speech disrupted the comprehensive aspect of the task, but the effect of meaningful speech was significantly greater. Both rehearsal and semantic processing, which are involved in reading comprehension, seem to be susceptible to disruption by irrelevant meaningful speech. The study provides some evidence to suggest that the presence of meaning in the irrelevant sound in creases disruption of performance in cognitive tasks that also call upon processing of meaning.

Identifying cortical inputs to the rat hippocampus that subserve allocentric spatial processes: a simple problem with a complex answer.

A consideration of the cortical projections to the hippocampus provides a number of candidate regions that might provide distal sensory information needed for allocentric processing. Prominent among the input regions are the entorhinal cortex, the perirhinal cortex, the postrhinal cortex, and the retrosplenial cortex. A review of these sites reveals the surprising fact that in spite of their anatomical connections, removal of the perirhinal and postrhinal cortices has little or no effect on spatial tasks and hence does not functionally disconnect the hippocampus. Extensive retrosplenial lesions have only mild effects, and even lesions of the entorhinal cortex only partially mimic the effects of hippocampal lesions upon tests of spatial memory. In contrast, studies using c-fos imaging support the involvement of the entorhinal, postrhinal, and retrosplenial cortices, but not the perirhinal cortex. It is argued that there exist multiple aspects of spatial memory, and this is reflected in the multiple routes by which cortical information can reach the hippocampus. One consequence is that lesions in a single site often have surprisingly mild effects on standard spatial tests.

The effects of combined lesions of the subicular complex and the entorhinal cortex on two forms of spatial navigation in the water maze.

The role of the subicular complex and entorhinal cortex (SUB-EC) in spatial learning was examined in 2 water maze experiments. In Experiment 1, rats had to locate a hidden platform that was always a fixed distance and direction from an intramaze landmark. Each day, the landmark and platform were moved to a new location. Both control and SUB-EC-lesioned rats learned to locate the platform equally readily during training. However, the control group was impaired in locating the platform when the visual extramaze cues were concealed, whereas the lesioned group was unaffected by this manipulation. In Experiment 2, the lesioned rats were impaired in finding a hidden platform that was in a fixed place in the water maze and showed no evidence of having learned its location in a probe test. These results suggest that damage to the SUB-EC impairs the integration of geometric information but spares a more general navigational-directional strategy.