ABSTRACT
One important application of clustered regulatory interspaced short palindromic repeats (CRISPR)/Cas 9 is the development of knock-out cell lines, specifically to study the function of new genes/proteins associated with a disease, identified during the genetic diagnosis. For the development of such cell lines, two major issues have to be untangled: insertion of the CRISPR tools (the Cas9 and the guide RNA) with high efficiency into the chosen cells, and restriction of the Cas9 activity to the specific deletion of the chosen gene. The protocol described here is dedicated to the insertion of the CRISPR tools in difficult to transfect cells, such as muscle cells. This protocol is based on the use of lentiviruses, produced with plasmids publicly available, for which all the cloning steps are described to target a gene of interest. The control of Cas9 activity has been performed using an adaptation of a previously described system called KamiCas9, in which the transduction of the cells with a lentivirus encoding a guide RNA targeting the Cas9 allows the progressive abolition of Cas9 expression. This protocol has been applied to the development of a RYR1-knock out human muscle cell line, which has been further characterized at the protein and functional level, to confirm the knockout of this important calcium channel involved in muscle intracellular calcium release and in excitation-contraction coupling. The procedure described here can easily be applied to other genes in muscle cells or in other difficult to transfect cells and produce valuable tools to study these genes in human cells.
Subject(s)
Gene Editing , RNA, Guide, Kinetoplastida , CRISPR-Cas Systems , Cell Line , Gene Editing/methods , Humans , Lentivirus/genetics , Muscle Cells , Muscles , RNA, Guide, Kinetoplastida/geneticsABSTRACT
Mutations in the RYR1 gene, encoding the skeletal muscle calcium channel RyR1, lead to congenital myopathies, through expression of a channel with abnormal permeability and/or in reduced amount, but the direct functional whole organism consequences of exclusive reduction in RyR1 amount have never been studied. We have developed and characterized a mouse model with inducible muscle specific RYR1 deletion. Tamoxifen-induced recombination in the RYR1 gene at adult age resulted in a progressive reduction in the protein amount reaching a stable level of 50% of the initial amount, and was associated with a progressive muscle weakness and atrophy. Measurement of calcium fluxes in isolated muscle fibers demonstrated a reduction in the amplitude of RyR1-related calcium release mirroring the reduction in the protein amount. Alterations in the muscle structure were observed, with fibers atrophy, abnormal mitochondria distribution and membrane remodeling. An increase in the expression level of many proteins was observed, as well as an inhibition of the autophagy process. This model demonstrates that RyR1 reduction is sufficient to recapitulate most features of Central Core Disease, and accordingly similar alterations were observed in muscle biopsies from Dusty Core Disease patients (a subtype of Central Core Disease), pointing to common pathophysiological mechanisms related to RyR1 reduction.
Subject(s)
Muscle Weakness/genetics , Muscle, Skeletal/metabolism , Muscular Atrophy/genetics , Myopathy, Central Core/genetics , Ryanodine Receptor Calcium Release Channel/genetics , Animals , Calcium/metabolism , Disease Models, Animal , Gene Knockdown Techniques , Mice , Mice, Transgenic , Mitochondria, Muscle/pathology , Muscle Fibers, Skeletal/metabolism , Muscle Fibers, Skeletal/pathology , Muscle Weakness/metabolism , Muscle Weakness/pathology , Muscle, Skeletal/pathology , Muscular Atrophy/metabolism , Muscular Atrophy/pathology , Myopathy, Central Core/metabolism , Myopathy, Central Core/pathology , Ryanodine Receptor Calcium Release Channel/metabolismABSTRACT
Dent-2 disease and Lowe syndrome are two pathologies caused by mutations in inositol polyphosphate 5-phosphatase OCRL gene. Both conditions share proximal tubulopathy evolving to chronic kidney failure. Lowe syndrome is in addition defined by a bilateral congenital cataract, intellectual disability, and hypotonia. The pathology evolves in two decades to a severe condition with renal complications and a fatal issue. We describe here a proof of principle for a targeted gene therapy on a mutation of the OCRL gene that is associated with Lowe syndrome. The affected patient bears a deep intronic mutation inducing a pseudo-exon inclusion in the mRNA, leading to a OCRL-1 protein loss. An exon-skipping strategy was designed to correct the effect of the mutation in cultured cells. We show that a recombinant U7-modified small RNA efficiently triggered the restoration of normal OCRL expression at mRNA and protein levels in patient's fibroblasts. Moreover, the PI(4,5)P2 accumulation and cellular alterations that are hallmark of OCRL-1 dysfunction were also rescued. Altogether, we provide evidence that the restoration of OCRL-1 protein, even at a reduced level, through RNA-based therapy represents a potential therapeutic approach for patients with OCRL splice mutations.
Subject(s)
Introns , Mutation , Oculocerebrorenal Syndrome/genetics , Oculocerebrorenal Syndrome/metabolism , Phosphoric Monoester Hydrolases/genetics , Phosphoric Monoester Hydrolases/metabolism , Alleles , Alternative Splicing , Amino Acid Substitution , Child, Preschool , Enzyme Activation , Exons , Fibroblasts , Genetic Association Studies , Genetic Predisposition to Disease , Genotype , Humans , Male , Molecular Imaging , Oculocerebrorenal Syndrome/diagnosis , PhenotypeABSTRACT
The molecular mechanisms and the biological functions of clathrin independent endocytosis (CIE) remain largely elusive. Alix (ALG-2 interacting protein X), has been assigned roles in membrane deformation and fission both in endosomes and at the plasma membrane. Using Alix ko cells, we show for the first time that Alix regulates fluid phase endocytosis and internalization of cargoes entering cells via CIE, but has no apparent effect on clathrin mediated endocytosis or downstream endosomal trafficking. We show that Alix acts with endophilin-A to promote CIE of cholera toxin and to regulate cell migration. We also found that Alix is required for fast endocytosis and downstream signaling of the interleukin-2 receptor giving a first indication that CIE is necessary for activation of at least some surface receptors. In addition to characterizing a new function for Alix, our results highlight Alix ko cells as a unique tool to unravel the biological consequences of CIE.
Subject(s)
Acyltransferases/metabolism , Calcium-Binding Proteins/metabolism , Cell Cycle Proteins/metabolism , Endocytosis/genetics , Endosomal Sorting Complexes Required for Transport/metabolism , Endosomes/metabolism , Receptors, Interleukin-2/metabolism , Acyltransferases/genetics , Animals , B-Lymphocytes/cytology , B-Lymphocytes/drug effects , B-Lymphocytes/metabolism , Calcium-Binding Proteins/genetics , Cell Cycle Proteins/genetics , Cell Line, Tumor , Cell Movement/drug effects , Cholera Toxin/metabolism , Cholera Toxin/toxicity , Clathrin/genetics , Clathrin/metabolism , Embryo, Mammalian , Endosomal Sorting Complexes Required for Transport/genetics , Endosomes/drug effects , Fibroblasts/cytology , Fibroblasts/drug effects , Fibroblasts/metabolism , Gene Expression , Humans , Mice , Mice, Knockout , Neurons/cytology , Neurons/drug effects , Neurons/metabolism , Primary Cell Culture , Protein Binding , Receptors, Interleukin-2/genetics , Signal TransductionABSTRACT
The cytoplasmic protein Alix/AIP1 (ALG-2 interacting protein X) is involved in cell death through mechanisms which remain unclear but require its binding partner ALG-2 (apoptosis-linked gene-2). The latter was defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. We show here that Alix is also a critical component of caspase 9 activation and apoptosis triggered by calcium. Indeed, expression of Alix dominant-negative mutants or downregulation of Alix afford significant protection against cytosolic calcium elevation following thapsigargin (Tg) treatment. The function of Alix in this paradigm requires its interaction with ALG-2. In addition, we demonstrate that caspase 9 activation is necessary for apoptosis induced by Tg and that this activation is impaired by knocking down Alix. Altogether, our findings identify, for the first time, Alix as a crucial mediator of Ca(2+) induced caspase 9 activation.
Subject(s)
Apoptosis , Calcium-Binding Proteins/metabolism , Calcium/metabolism , Caspase 9/metabolism , Animals , Calcium/pharmacology , Calcium-Binding Proteins/genetics , Cell Line , Cricetinae , Enzyme Activation , Enzyme Inhibitors/pharmacology , Humans , Mice , Mice, Inbred Strains , Thapsigargin/pharmacologyABSTRACT
Alix [ALG-2 (apoptosis-linked gene 2)-interacting protein X] is a ubiquitinous adaptor protein first described for its capacity to bind to the calcium-binding protein, ALG-2. Alix regulates neuronal death in ways involving interactions with ALG-2 and with proteins of the ESCRT (endosomal sorting complex required for transport). Even though all Alix interactors characterized to date are involved in endosomal trafficking, the genuine function of the protein in this process remains unclear. We have demonstrated recently that Alix and ALG-2 form in the presence of calcium, a complex with apical caspases and with the endocytosed death receptor TNFR1 (tumour necrosis factor alpha receptor 1), thus suggesting a molecular coupling between endosomes and the cell death machinery.
Subject(s)
Calcium-Binding Proteins/metabolism , Endosomes/metabolism , Neurons/cytology , Animals , Caspases/metabolism , Cell Death , Endosomes/enzymology , Enzyme Activation , Humans , Neurons/enzymologyABSTRACT
Alix and ESCRT proteins are required for membrane fission during viral budding and egress and during the abscission stage of cytokinesis. These common roles have suggested that Alix functions as an ESCRT protein, a conclusion challenged by the finding that unlike ESCRTs, which control the formation of multivesicular endosomes, Alix does not influence the degradation of the EGF receptor. We previously showed that Alix controls neuronal death by an unknown mechanism, but dependent on its interaction with ESCRT proteins. Since then, numerous reports have shown that ESCRTs participate in macroautophagy. Given the direct interaction between ESCRTs and Alix, together with the known contribution of autophagy to cell death, it was hypothesized that Alix controls autophagy and thereby cell death. Our recent published results show that this is not the case. ESCRT protein activity therefore needs Alix for viral budding and cytokinesis but not for autophagy. The function of ESCRT can thus be clearly be disconnected from that of Alix.
Subject(s)
Autophagy , Calcium-Binding Proteins/metabolism , Endosomes/metabolism , Multiprotein Complexes/metabolism , Animals , Biological Transport , Caspases/metabolism , HumansABSTRACT
Alix/AIP1 regulates cell death in a way involving interactions with the calcium-binding protein ALG-2 and with proteins of ESCRT (endosomal sorting complex required for transport). Using mass spectrometry we identified caspase-8 among proteins co-immunoprecipitating with Alix in dying neurons. We next demonstrated that Alix and ALG-2 interact with pro-caspase-8 and that Alix forms a complex with the TNFalpha receptor-1 (TNF-R1), depending on its capacity to bind ESCRT proteins. Thus, Alix and ALG-2 may allow the recruitment of pro-caspase-8 onto endosomes containing TNF-R1, a step thought to be necessary for activation of the apical caspase. In line with this, expression of Alix deleted of its ALG-2-binding site (AlixDeltaALG-2) significantly reduced TNF-R1-induced cell death, without affecting endocytosis of the receptor. In a more physiological setting, we found that programmed cell death of motoneurons, which can be inhibited by AlixDeltaALG-2, is regulated by TNF-R1. Taken together, these results highlight Alix and ALG-2 as new actors of the TNF-R1 pathway.
Subject(s)
Apoptosis Regulatory Proteins/metabolism , Calcium-Binding Proteins/metabolism , Cell Cycle Proteins/metabolism , Receptors, Tumor Necrosis Factor, Type I/metabolism , Animals , Binding Sites , Caspase 8/metabolism , Cell Death , Chick Embryo , Endocytosis , Endosomal Sorting Complexes Required for Transport , Endosomes/metabolism , Humans , Neural Crest/embryology , Neurons/metabolism , Protein Structure, Tertiary , Recombinant Proteins/chemistryABSTRACT
Alix/AIP1 is a cytosolic protein that regulates cell death through mechanisms that remain unclear. Alix binds to two protein members of the so-called Endosomal Sorting Complex Required for Transport (ESCRT), which facilitates membrane fission events during multivesicular endosome formation, enveloped virus budding and cytokinesis. Alix itself has been suggested to participate in these cellular events and is thus often considered to function in the ESCRT pathway. ESCRT proteins were recently implicated in autophagy, a process involved in bulk degradation of cytoplasmic constituents in lysosomes, which can also participate in cell death. In this study, we shown that, unlike ESCRT proteins, Alix is not involved in autophagy. These results strongly suggest that the capacity of several mutants of Alix to block both caspase-dependent and independent cell death does not relate to their capacity to modulate autophagy. Furthermore, they reinforce the conclusion of other studies demonstrating that the role of Alix is different from that of classical ESCRT proteins.
Subject(s)
Autophagy , Calcium-Binding Proteins/metabolism , Cell Cycle Proteins/metabolism , Endosomes/metabolism , Animals , Autophagy/genetics , Calcium-Binding Proteins/genetics , Cell Cycle Proteins/genetics , Cell Line , Cricetinae , Endosomal Sorting Complexes Required for Transport , Humans , Microtubule-Associated Proteins/metabolism , Mutation , Vacuoles/metabolismABSTRACT
During viral infection, fusion of the viral envelope with endosomal membranes and nucleocapsid release were thought to be concomitant events. We show here that for the vesicular stomatitis virus they occur sequentially, at two successive steps of the endocytic pathway. Fusion already occurs in transport intermediates between early and late endosomes, presumably releasing the nucleocapsid within the lumen of intra-endosomal vesicles, where it remains hidden. Transport to late endosomes is then required for the nucleocapsid to be delivered to the cytoplasm. This last step, which initiates infection, depends on the late endosomal lipid lysobisphosphatidic acid (LBPA) and its putative effector Alix/AIP1, and is regulated by phosphatidylinositol-3-phosphate (PtdIns3P) signalling via the PtdIns3P-binding protein Snx16. We conclude that the nucleocapsid is exported into the cytoplasm after the back-fusion of internal vesicles with the limiting membrane of late endosomes, and that this process is controlled by the phospholipids LBPA and PtdIns3P and their effectors.
Subject(s)
Cytosol/metabolism , Endosomes/metabolism , Membrane Fusion/physiology , Nucleocapsid/metabolism , Animals , Biological Transport/physiology , Cattle , Cell Line , Cricetinae , Cytosol/ultrastructure , Endosomal Sorting Complexes Required for Transport , Endosomes/ultrastructure , Epithelial Cells/virology , Fibroblasts/virology , HeLa Cells , Humans , Lysophospholipids/physiology , Membrane Fusion/drug effects , Microscopy, Electron , Microscopy, Fluorescence , Monoglycerides , Phosphatidylinositol Phosphates/physiology , Phosphoproteins/genetics , Phosphoproteins/physiology , RNA, Viral/biosynthesis , RNA, Viral/metabolism , Signal Transduction/physiology , Sorting Nexins , Time Factors , Transport Vesicles/metabolism , Transport Vesicles/ultrastructure , Vesicular Transport Proteins/genetics , Vesicular Transport Proteins/physiology , Vesicular stomatitis Indiana virus/physiology , Virus Replication/geneticsABSTRACT
Macroautophagy or autophagy is an ubiquitous and conserved degradative pathway of cytosolic components, macromolecules or organelles, into the lysosome. By using biochemical and microscopic methods, which allow one to measure the rate of autophagy, the role of two regulators of Gi3 protein activity, activator of G-protein-signaling-3 (AGS3) and Galpha-interacting protein (GAIP), was studied in the control of autophagy in human colon cancer HT-29 cells. In HT-29 cells, autophagy is under the control of the Gi3 protein and, when bound to the GTP, the Galphai3 protein inhibits autophagy, whereas it stimulates autophagy when bound to the GDP. GAIP, which enhances the intrinsic GTPase-activating protein activity of the Galphai3 protein, stimulates autophagy by favoring the GDP-bound form of Galphai3. We showed that GAIP is phosphorylated on its serine 151 and that this phosphorylation is dependent on the presence of amino acids that modulate Raf-1 activity, the kinase upstream of Erk1/2. AGS3, a guanine nucleotide dissociation inhibitor, stimulates autophagy by binding Galphai3 proteins. The intracellular localization of AGS3 (Golgi apparatus and endoplasmic reticulum, two membranes known to be at the origin of autophagosomes) is consistent with its role in autophagy.
Subject(s)
Autophagy/physiology , Cadaverine/analogs & derivatives , Carrier Proteins/metabolism , Phosphoproteins/metabolism , Animals , Cadaverine/metabolism , Cell Line, Tumor , Colonic Neoplasms , Fluorescent Dyes/metabolism , GTP-Binding Protein alpha Subunits, Gi-Go/metabolism , Humans , Intestinal Mucosa/cytology , Intestinal Mucosa/metabolism , L-Lactate Dehydrogenase/metabolism , Microtubule-Associated Proteins/metabolism , Phosphorylation , RGS Proteins , Vacuoles/chemistry , Vacuoles/metabolismABSTRACT
Macroautophagy is a major lysosomal catabolic process conserved from yeast to human. The formation of autophagic vacuoles is stimulated by a variety of intracellular and extracellular stress situations including amino acid starvation, aggregation of misfolded proteins, and accumulation of damaged organelles. Several signaling pathways control the formation of autophagic vacuoles. As some of them are engaged in the control of protein synthesis or cell survival this suggests that macroautophagy is intimately associated with the execution of cell proliferation and cell death programs. Whether or not these different signaling pathways converge to a unique point to trigger the formation of autophagic vacuole remains an open question.