[Constitutional anomalies of hemostasis and management in surgical situation. Evaluation of 10 years' activity at the Hospital Centers of Aulnay-sous-Bois and Saint-Denis]. / Anomalies constitutionnelles de l'hémostase et conduite à tenir en situation chirurgicale. Bilan de dix ans d'activité aux Centres Hospitaliers d'Aulnay-sous-Bois et de Saint-Denis.

A ten-year retrospective study was conducted in 4 women in labor and 23 surgical patients with congenital clotting factor deficiency. Appropriate use of replacement therapy and good cooperation between biologists, anesthesiologists, and surgeons are essential to ensure a favorable outcome.

[Diagnostic and prognostic importance of laboratory tests in malaria in airports. Study of six recent cases]. / Importance diagnostique et pronostique des examens de laboratoire au cours du paludisme des aéroports. Etude de six cas récents.

During the summer 1994, six cases of airport malaria occurred in France, near the Roissy-Charles-de-Gaulle airport. Due to Plasmodium falciparum, all cases underwent rapid and severe deterioration, and in one case, the patient died. The role of laboratory tests is essential to establish the diagnosis of persons who have never resided in the endemic malaria areas and follow up with the patients already under treatment to detect possible complications.

Schistosoma mansoni: protein composition and synthesis during early development; evidence for early synthesis of heat shock proteins.

There are alterations in the proteins synthesized during different stages of development of Schistosoma mansoni. The protein profiles at different stages were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. When stained by Coomassie blue, no significant differences were seen in protein profiles derived from cultured schistosomula from Days 0 to 6 and from adults. Newly synthesized proteins were detected by [35S]methionine incorporation. There were only a few differences in the protein profiles of schistosomula from Days 1 to 6 and from adults. Profiles derived from Day 0 schistosomula showed striking differences. Only a few proteins appear to be synthesized on Day 0 under these conditions. Schistosomula on Day 0 synthesized several minor proteins as well as a major protein of approximately 69,000 Da. This protein was immunoprecipitated by rabbit antiserum against bovine uncoating adenosine triphosphatase which recognizes the constitutive and induced 70,000 Da heat shock proteins in a wide variety of eukaryotic cells. More significant differences were observed when the newly synthesized proteins were analyzed by two dimensional gel electrophoresis. The profiles of newly synthesized proteins showed a specific repertoire of expression during the early stages of development in the parasite. A shift in temperature and medium during transformation from aquarium water to isotonic medium may initiate the synthesis of heat shock protein in these parasites.

[Sero-immunologic diagnosis of human African trypanosomiasis: cross reactions of various parasitoses and hyperglobulinemias]. / Le diagnostic séro-immunologique de la trypanosomose africaine humaine: réactions croisées au cours de diverses parasitoses et hyperglobulinémies.

The immunological test: latex agglutination, counter immuno-electrophoresis, ELISA, and immunoperoxydase test, have been applied on the serodiagnosis of african sleeping sickness, and have been compared with each other, with special reference to the cross-reactions in parasitic diseases and hyperglobulinemia. In addition to malaria and leishmaniasis well known interferences, this study gives clear indication of the importance of false positive reactions in active toxoplasma infection. Any how, the serodiagnostic can be worked out from the analysis of responses to various specific antigens, which are always more positive with the homologous antigen.

[Blood proteins in African trypanosomiasis: variations and statistical interpretations]. / Les protéines sériques dams la trypamosomose africaine humaine: variations et interprétation statistique.

The estimation of blood orosomucoid, haptoglobin, C-reactive protein and immunoglobulins levels, has enable us to prove a specific proteic profile in the human african trypanosomiasis, as compared with other that of parasitic diseases, and with an healthy african reference group. Data processing informatique by principal components analysis, provide a valuable pool for epidemiological surveys.

[Use of multidimensional technics of analysis in the study of specific immunoglobulins in human African trypanosomiasis]. / Utilisation des techniques d'analyse multidimensionnelle dans l'étude des immunoglobulines spécifiques au cours de la trypanosomose africaine humaine.

In human african trypanosomiasis, the quantitative studies of specific immunoglobulins (IgG, IgA, IgM) complement fixing antibody, and multivariate analysis methods (discriminant analysis allocation of cases with a Jacknife procedure) enable to assert the immunological profiles evolution through the different stages of the disease.

[Comparative study of immunoenzyme technics, ELISA and on formed antigens, in the immunologic diagnosis of African trypanosomiasis]. / Etude comparative des techniques immunoenzymatiques, ELISA et sur antigène figuré, dans le diagnostic immunologique des trypanosomoses africaines.

The enzyme linked immunosorbent assay (ELISA) for the serodiagnosis of human african trypanosomiasis is described, comparatively with the immunoperoxidase technique using blood streamforms of Trypanosoma brucei as antigen. Practical aspects for measuring the amount of antibody in serum samples from infected rabbits and from patients with african sleeping sickness are discussed. ELISA does offer important advantages over the other method used namely: -- quantitative estimates are possible, -- it bends itself readily to large scale series, owing the fact that it can be fully automatic. These qualities will be turned to full account when the different classes of antibodies present in trypanosomiasis suffers can be examined by means of specific antigenic fractions.

[Comparative value of immunofluorescence and immunoenzymology on figured antigen in the immunological diagnosis of African trypanosomiasis]. / Valeur comparée de l'immunofluorescence et de l'immunoenzymologie sur antigène figuré dans le diagnostic immunologique des trypanosomoses africaines.

Indirect immunoperoxydase method is compared with indirect immunofluorescence using immunized animal and human sera, and negative control sera. The obtained results show a good statisticly established concord and allow to propose the indirect immunoperoxydase method being used in the biological diagnostic of african Trypanosomiasis because its easy carrying out and its examin with an ordinary microscope.

[Electrosyneresis in the diagnosis of African trypanosomiasis]. / Les possibilités de l'électrosynérése dans le diagnostic des trypanosomoses africaines

Comparative studies were carried out on serums from rabbits inoculated with several Trypanosoma strains and 50 serums from sleeping sickness cases. The techniques used were antigen-antibody reaction in gel according to Ouchterlony, and electrosynerese for which directions are outlined. Electrosynerese has been shown to be a much faster and more sensitive method of detecting precipitating antibodies in trypanosomiasis.

[Preparation of a standardized antigenic extract from several strains of African trypansomes]. / Préparation d'un extrait antigénique standardisé à partir de diverses souches de trypansomes africains

The preparation of a soluble antigen from african trypanosomes is described below. It is important to observe the following steps: --minimal dose of the phytohemagglutinin must be placed in rapid contact with the trypanosomes; --the standard speed of the different centrifugations must be strictly observed; --a thorough washing of the trypanosomes must be carried out to eliminate the hetero-antigens, but caution should be excercised, as over washing is harmful to the Trypanosomes.