1.
J Fungi (Basel)
; 7(7)2021 Jun 24.
Article
in English
| MEDLINE
| ID: mdl-34202579
ABSTRACT
Monoclonal antibody EBCA-1 is used in the sandwich immune assay for the detection of circulating Candida mannan in blood sera samples for the diagnosis of invasive candidiasis. To reinvestigate carbohydrate specificity of EBCA-1, a panel of biotinylated oligosaccharides structurally related to distinct fragments of Candida mannan were loaded onto a streptavidin-coated plate to form a glycoarray. Its use demonstrated that EBCA-1 recognizes the trisaccharide ß-Man-(1â2)-α-Man-(1â2)-α-Man and not homo-α-(1â2)-linked pentamannoside, as was reported previously.