Label-free electrochemical biosensor based on dual amplification of gold nanoparticles and polycaprolactones for CEA detection.

Carcinoembryonic Antigen (CEA), an acidic glycoprotein with human embryonic antigen properties, is found on the surface of cancer cells that have differentiated from endodermal cells. This paper presents a label-free electrochemical immunoassay for the dual amplification detection of CEA using gold nanoparticles loaded with polypyrrole polydopamine (Au/PPy-PDA) and polymerized polycaprolactone (Ng-PCL) prepared by ring-opening polymerization (ROP). First, the composite Au/PPy-PDA was adhered to the electrode surface. Then, gold nanoparticles form a Au-S bond with the sulfhydryl group in Apt1 to secure it on the electrode surface. Subsequently, the non-specific binding sites on the electrodes surface are closed by bovine serum albumin (BSA). Next, CEA is dropped onto the electrode surface, which is immobilized by antigen-antibody specific recognition, and the carboxyl-functionalized Apt2 forms a "sandwich structure" of antibody-antigen-antibody by specific recognition. Polymeric Ng-PCL is adhered to the electrode surface, leading to an increase in the electrochemical impedance signal, resulting in a complete chain of signal analysis. Finally, the response signal is detected by electrochemical impedance spectroscopy (EIS). Under optimal experimental conditions, the method has the advantages of high sensitivity and wide linear range (1 pg mL-1∼100 ng mL-1), and the lower limit of detection (LOD) is 0.234 pg mL-1. And it has the same high sensitivity, selectivity and interference resistance for the real samples detection. Thus, it provides a new way of thinking about biomedical and clinical diagnosis.

Reactive Oxygen and Related Regulatory Factors Involved in Ethylene-Induced Petal Abscission in Roses.

Petal abscission affects the growth, development, and economic value of plants, but the mechanism of ethylene-ROS-induced petal abscission is not clear. Therefore, we treated roses with different treatments (MOCK, ETH, STS, and ETH + STS), and phenotypic characteristics of petal abscission, changed ratio of fresh weight, morphology of cells in AZ and the expression of RhSUC2 were analyzed. On this basis, we measured reactive oxygen species (ROS) content in petals and AZ cells of roses, and analyzed the expression levels of some genes related to ROS production and ROS scavenging. Ethylene promoted the petal abscission of rose through decreasing the fresh weight of the flower, promoting the stacking and stratification of AZ cells, and repressing the expression of RhSUC2. During this process, ethylene induced the ROS accumulation of AZ cells and petals mainly through increasing the expressions of some genes (RhRHS17, RhIDH1, RhIDH-III, RhERS, RhPBL32, RhFRS5, RhRAC5, RhRBOHD, RhRBOHC, and RhPLATZ9) related to ROS production and repressing those genes (RhCCR4, RhUBC30, RhSOD1, RhAPX6.1, and RhCATA) related to ROS scavenging. In summary, ROS and related regulatory factors involved in ethylene induced petal abscission in roses.

METTL5 promotes gastric cancer progression via sphingomyelin metabolism.

BACKGROUND: The treatment of gastric cancer (GC) has caused an enormous social burden worldwide. Accumulating studies have reported that N6-methyladenosine (m6A) is closely related to tumor progression. METTL5 is a m6A methyltransferase that plays a pivotal role in maintaining the metabolic stability of cells. However, its aberrant regulation in GC has not been fully elucidated. AIM: To excavate the role of METTL5 in the development of GC. METHODS: METTL5 expression and clinicopathological characteristics were analyzed via The Cancer Genome Atlas dataset and further verified via immunohistochemistry, western blotting and real-time quantitative polymerase chain reaction in tissue microarrays and clinical samples. The tumor-promoting effect of METTL5 on HGC-27 and AGS cells was explored in vitro by Cell Counting Kit-8 assays, colony formation assays, scratch healing assays, transwell assays and flow cytometry. The tumor-promoting role of METTL5 in vivo was evaluated in a xenograft tumor model. The EpiQuik m6A RNA Methylation Quantification Kit was used for m6A quantification. Next, liquid chromatography-mass spectrometry was used to evaluate the association between METTL5 and sphingomyelin metabolism, which was confirmed by Enzyme-linked immunosorbent assay and rescue tests. In addition, we investigated whether METTL5 affects the sensitivity of GC cells to cisplatin via colony formation and transwell experiments. RESULTS: Our research revealed substantial upregulation of METTL5, which suggested a poor prognosis of GC patients. Increased METTL5 expression indicated distant lymph node metastasis, advanced cancer stage and pathological grade. An increased level of METTL5 correlated with a high degree of m6A methylation. METTL5 markedly promotes the proliferation, migration, and invasion of GC cells in vitro. METTL5 also promotes the growth of GC in animal models. METTL5 knockdown resulted in significant changes in sphingomyelin metabolism, which implies that METTL5 may impact the development of GC via sphingomyelin metabolism. In addition, high METTL5 expression led to cisplatin resistance. CONCLUSION: METTL5 was found to be an oncogenic driver of GC and may be a new target for therapy since it facilitates GC carcinogenesis through sphingomyelin metabolism and cisplatin resistance.

The alterations of functional brain networks and its relationship with sport decision-making and training duration in soccer players across different skill levels.

Studies have indicated that skilled soccer players possess superior decision-making abilities compared to their less-skilled counterparts. However, the underlying neural mechanism for this phenomenon remains incompletely understood. In our investigation, we explored distinctions in the topology of functional brain networks between skilled and less-skilled soccer players. Employing mediating analysis, we scrutinized the relationships among functional brain network parameters, training duration, and decision-making accuracy. Our findings revealed that skilled soccer players demonstrated significantly higher decision-making accuracy compared to their less-skilled counterparts. Skilled players also exhibited increased values in the cluster coefficient, characteristic path length and local efficiency but lower global efficiency. Moreover, we observed enhanced functional brain connectivity within the occipital and cingulo-opercular networks, as well as between the fronto-parietal and cingulo-opercular networks in skilled soccer players. Cluster coefficient and functional connectivity between fronto-parietal and cingulo-opercular networks had positive mediating effects on the association between training duration and sport decision-making accuracy. In conclusion, our study provides initial evidence for distinctions in functional brain network parameters between soccer players with varying skill levels and their relationship with sport decision-making accuracy.

Measurement Method for Contact Wire Wear Based on Stereovision.

The contact wire wear is an important parameter to ensure the safety operation of electric railways. The contact wire may break if the wear is serious, which leads to transportation interruptions. This study proposes an optical measurement method of contact wire wear, using stereovision technology. The matching method of stereovision based on line-scan cameras is proposed. A lookup-table method is developed to exactly determine the image resolution caused by the contact wire being in different spatial positions. The wear width of the contact wire is extracted from catenaries' images, and the residual thickness of the contact wire is calculated. The method was verified by field tests. The round-robin tests of the residual thickness at the same location present excellent measurement repetitiveness. The maximum difference value between dynamic test results and ground measurement results is 0.13 mm. This research represents a potential way to implement condition-based maintenance for contact wire wear in the future in order to improve the maintenance efficiency and ensure the safety of catenary infrastructure.

Temporal features of goals, substitutions, and fouls in football games in the five major European league from 2018 to 2021.

The "Big Five" European football leagues, comprising England's Premier League, Germany's Bundesliga, Spain's La Liga, Italy's Serie A, and France's Ligue 1, command significant attention. While the occurrence of goals, substitutions, and fouls in football games is often considered random, of the presence of an inherent inevitability is unclear. To investigate, we analyzed a public dataset detailing timing of goals, substitutions, and yellow cards in regular time from WhoScored across three seasons (2018-2019, 2019-2020, 2020-2021) in the top five European football leagues. We employed various mathematical descriptive models (including linear, sigmoid, and gaussian functions) to measure the temporal tendency of goals, substitutions, and yellow cards. Our results indicate that, whether in the first or second half of the match, the temporal distribution of these elements exhibits evenness a (indicative of randomness). However, specific characteristics were discerned through distinct model parameters, capturing novel phenomena that were intuitively illustrated. Furthermore, we explored the interaction of the timing of goals, substitutions, and yellow cards. In this analysis we found that scoring in the second half leads to more substitutions and yellow cards. Changing players in the second half corresponded with more goals, while the impact of yellow card fouls showed no differences in goals in the first and second halves. Our research is the first to systematically study the laws of modern football matches, providing valuable guidance and reference for many football coaches.

Progressive increase of brain gray matter volume in individuals with regular soccer training.

The study aimed to investigate alterations in gray matter volume in individuals undergoing regular soccer training, using high-resolution structural data, while also examining the temporal precedence of such structural alterations. Both voxel-based morphometry and source-based morphometry (SBM) methods were employed to analyze volumetric changes in gray matter between the soccer and control groups. Additionally, a causal network of structural covariance (CaSCN) was built using granger causality analysis on brain structural data ordering by training duration. Significant increases in gray matter volume were observed in the cerebellum in the soccer group. Additionally, the results of the SBM analysis revealed significant increases in gray matter volume in the calcarine and thalamus of the soccer group. The analysis of CaSCN demonstrated that the thalamus had a prominent influence on other brain regions in the soccer group, while the calcarine served as a transitional node, and the cerebellum acted as a prominent node that could be easily influenced by other brain regions. In conclusion, our study identified widely affected regions with increased gray matter volume in individuals with regular soccer training. Furthermore, a temporal precedence relationship among these regions was observed.

A combined transcriptomics and proteomics approach to reveal the mechanism of AEE relieving hyperlipidemia in ApoE-/- mice.

Hyperlipidemia caused by abnormal lipid metabolism has reached epidemic proportions. This phenomenon is also common in companion animals. Previous studies showed that AEE significantly improves abnormal blood lipids in hyperlipidemia rats and mice, but its mechanism is still not clear enough. In this study, the mechanism and potential key pathways of AEE on improving hyperlipidemia in mice were investigated through the transcriptome and proteome study of ApoE-/- mice liver and the verification study on high-fat HepG2 cells. The results showed that AEE significantly decreased the serum TC and LDL-C levels of hyperlipidemia ApoE-/- mice, and significantly increased the enzyme activity of CYP7A1. After AEE intervention, the results of mice liver transcriptome and proteome showed that differential genes and proteins were enriched in lipid metabolism-related pathways. The results of RT-qPCR showed that AEE significantly regulated the expression of genes related to lipid metabolism in mice liver tissue. AEE significantly upregulated the protein expression of CYP7A1 in hyperlipidemia ApoE-/- mice liver tissue. The results in vitro showed that AEE significantly decreased the levels of TC and TG, and improved lipid deposition in high-fat HepG2 cells. AEE significantly increased the expression of CYP7A1 protein in high-fat HepG2 cells. AEE regulates the expression of genes related to lipid metabolism in high-fat HepG2 cells, mainly by FXR-SHP-CYP7A1 and FGF19-TFEB-CYP7A1 pathways. To sum up, AEE can significantly improve the hyperlipidemia status of ApoE-/- mice and the lipid deposition of high-fat HepG2 cells, and its main pathway is probably the bile acid metabolism-related pathway centered on CYP7A1.

MicroRNA in the Exosomes Mediated by Resveratrol to Activate Neuronal Cells.

Resveratrol (RSV), a polyphenol, is known to have a wide range of pharmacological properties in vitro. RSV may have therapeutic value for various neurodegenerative diseases via neuroprotective effects. However, it is not yet clear whether RSV can induce intestinal-brain interactions. It is assumed that the intestinal cells may secrete some factors after being stimulated by other substances. These secreted factors may activate nerve cells through gut-brain interaction, such as exosomes. In this study, it was discovered that Caco-2 cells treated with RSV secrete exosomes to activate SH-SY5Y neuronal cells. The results showed that secreted factors from RSV-treated Caco-2 cells activated SH-SY5Y. The exosomes of RSV-treated Caco-2 cells activated SH-SY5Y cells, which was manifested in the lengthening of the nerve filaments of SH-SY5Y cells. The exosomes were characterized using transmission electron microscopy and sequenced using the Illumina NovaSeq 6000 sequencer. The results showed that the miRNA expression profile of exosomes after RSV treatment changed, and twenty-six kinds of miRNAs were identified which expressed differentially between the control group and the RSV-treated group. Among them, three miRNAs were selected as candidate genes for inducing SH-SY5Y neural cell activation. Three miRNA mimics could activate SH-SY5Y neurons. These results suggested that the miRNA in intestinal exocrine cells treated with RSV may play an important role in the activation of SH-SY5Y neurons.

Startup of a large height-diameter ratio bioreactor by alternate feeding: performance of partial nitrification and enrichment of ammonia-oxidizing bacteria (AOB).

In order to solve the complicated control of dissolved oxygen (DO) for partial nitrification in bioreactors treating high NH4+-N wastewater, a large height-diameter ratio anammox pre-reactor system was developed. And in this reactor, NO2--N accumulation rate can reach 85.76% by alternate feeding with high NH4+-N wastewater (150 mg NH4+-N/L) and low NH4+-N wastewater (50 mg c) with low DO (0.19 mg/L-0.62 mg/L). Based on 16S rRNA identification technology, it was found that Nitrosomonas had a significant effect on NH4+-N oxidization in this study. And when the reactor treated higher concentration wastewater (250 mg NH4+-N/L), the growth rate of Nitrosomonas was higher than that of Nitrospira (nitrite-oxidizing bacteria, NOB), which was conducive to improving the NO2--N accumulation rate and realizing partial nitrification stably. It was also found that the material exchange frequency of the microbial flora during alternate feeding with different NH4+-N concentration wastewaters was higher than that during feeding with higher NH4+-N concentration wastewater (250 mg/L) by Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolism pathways analysis. This study can provide valuable insights and lay the foundation for building anammox pre-reactors.

Synthesis and antibacterial activity of FST and its effects on inflammatory response and intestinal barrier function in mice infected with Escherichia coli O78.

Pathogenic Escherichia coli (E. coli) can cause intestinal diseases in humans and livestock, damage the intestinal barrier, increase systemic inflammation, and seriously threaten human health and the development of animal husbandry. In this study, we designed and synthesized a novel conjugate florfenicol sulfathiazole (FST) based on drug combination principles, and investigated its antibacterial activity in vitro and its protective effect on inflammatory response and intestinal barrier function in E. coli O78-infected mice in vivo. The results showed that FST had superior antibacterial properties and minimal cytotoxicity compared with its prodrugs as florfenicol and sulfathiazole. FST protected mice from lethal E. coli infection, reduced clinical signs of inflammation, reduced weight loss, alleviated intestinal structural damage. FST decreased the expression of inflammatory cytokines IL-1ß, IL-6, TNF-α, and increased the expression of claudin-1, Occludin, and ZO-1 in the jejunum, improved the intestinal barrier function, and promoted the absorption of nutrients. FST also inhibited the expression of TLR4, MyD88, p-p65, and p-p38 in the jejunum. The study may lay the foundation for the development of FST as new drugs for intestinal inflammation and injury in enteric pathogen infection.

DKK1 Promotes Epithelial-Mesenchymal Transition and Cisplatin Resistance in Gastric Cancer via Activation of the PI3K/AKT Pathway.

Chemotherapy is a classical method of cancer treatment. Cisplatin-based chemotherapy is a traditional and essential therapeutic approach in gastric cancer treatment. However, the development of drug resistance during treatment is a major obstacle that limits their further application, and molecular changes have occurred in the development of drug resistance. Here, we found that Dickkopf-related protein 1 (DKK1) is highly expressed in gastric cancer and related to poor prognosis in gastric cancer patients through public database mining. Next, we also identified that DKK1 is highly expressed in CDDP-resistant gastric cancer cell lines, supporting the notion that DKK1 is a necessary regulator of CDDP resistance. In terms of mechanistic research, our data reveal that DKK1 was able to activate the PI3K/AKT pathway and affect epithelial-to-mesenchymal transition, further contributing to CDDP resistance. Genetic knockdown and pharmacological inhibition of DKK1 recovered CDDP sensitivity both in vitro and in vivo. Therefore, our study highlights the potential of targeted inhibition of DKK1 to reverse CDDP resistance and alleviate metastatic properties in gastric cancer.

[Research progress in clinical application and pharmacological effect of Yiyi Fuzi Baijiang Powder].

Yiyi Fuzi Baijiang Powder(YFBP), originating from Synopsis of the Golden Chamber, is a classic prescription composed of Coicis Semen, Aconiti Lateralis Radix Praeparata, and Patriniae Herba for the treatment of abscesses and pus discharge. This article presented a systematic analysis of the clinical application of YFBP, including the indicated diseases, the number of cases, efficacy, dosage, administration methods, and compatibility with other drugs. The analysis reveals that YFBP has a wide range of clinical applications. It is commonly used, often with modifications or in combination with western medicine, for diseases in the fields of gastroente-rology, gynecology, urology, dermatology, and others. And most of the Traditional Chinese Medicine(TCM) evidence involved in these diseases are damp-heat evudence. The prescription shows rich variations in clinical administration methods, and most of which are the treatment of aqueous decoction of it. The therapeutic effect is also significant, and the total effective rate of clinical treatment is re-latively high. Additionally, this article summarized the pharmacological research on YFBP and found that it possessed various pharmacological effects, including anti-inflammatory, antioxidant, anticancer, and immune-modulating properties. Finally, correlation analysis was conducted on the main diseases, TCM types, prescription doses, pharmacological effects and action targets of YFBP, which to show the relationship between these five aspects in a visual form, reflecting the relationship between its clinical application and modern pharmacological effects. These findings provide a reference basis for further development and research on YFBP.

Characteristics of brain activation in high-level football players at different stages of decision-making tasks off the ball: an fMRI study.

Objective: This study aimed to examine the neural mechanisms underlying the decision-making process of off-ball movements among high-level football players and ordinary college students, as well as the effect of long-term skill training on these neural mechanisms using functional magnetic resonance imaging (fMRI). Methods: The study recruited 20 professional college football players as the expert group (EG) and 20 novice football players with no background in sports-related disciplines as the novice group (NG). The participants performed the motor video observation and button-decision-making tasks, and fMRI data were acquired, pre-processed, and analyzed. Results: During the decision-making process regarding running without the ball, whole-brain fMRI scans were conducted on both the EG and NG. The analysis of these scans revealed noteworthy disparities in brain activity between the two groups. These disparities were observed during tasks involving motor video observation and button-based decision-making. According to the behavioral data, the EG made more correct decisions than the NG (p < 0.05); however, there was no significant difference in their reaction speed (p > 0.05). During video observation, both the EG and NG exhibited simultaneous activation in the frontoparietal cognitive area, primary somatosensory cortex, visual cortex, and insula. However, there were no significant differences between the two groups in terms of activated brain regions [false discovery rate (FDR) corrected to p < 0.05]. Regarding button-press decisions, the areas of the brain that were commonly activated in both the NG and EG were primarily located in the frontoparietal cognitive area, temporal cortex, and cuneus cortex. Notably, the left superior temporal gyrus, left inferior temporal gyrus, and left middle occipital gyrus exhibited greater activation in the NG compared to those in the EG (FDR corrected to p < 0.05). Conclusion: This study demonstrated that during motor video observation, the EG's sports experience and professional knowledge can help them achieve better visual information processing strategies in specific areas of sports. During button decision-making, the EG was more economical, whereas the NG required more brain function activity to process visual information, confirming the "neural efficiency" hypothesis.

AEE alleviates ox-LDL-induced lipid accumulation and inflammation in macrophages.

Atherosclerosis is a chronic immune inflammatory disease. Aspirin eugenol ester (AEE) is a novel safe and non-toxic compound with many pharmacological effects such as anti-inflammatory, anti-hyperlipidemic and anti-thrombotic action. In order to investigate the effect of AEE on the inhibition of aortic lipid plaque formation and macrophage-derived foam cell formation induced by oxidized low density lipoprotein (ox-LDL), in vivo atherosclerosis model by feeding ApoE-/- mice with a high-fat diet and foam cells formation in vitro model by ox-LDL-induced RAW264.7 macrophages were established. It was found that AEE decreased the levels of TC and LDL-C in serum, and the plaque formation area and lipid accumulation in the aortic intima of ApoE-/- mice. In vitro studies showed that AEE could prevent the uptake of ox-LDL and reduce the contents of TC and FC in cells. AEE enhanced the cholesterol efflux by increasing the expression of ABCA1, ABCG1 and PPARγ, which effectively alleviated excess cholesterol accumulated in the cells. Meanwhile, AEE also reduced the secretion and expression of inflammatory factors in the cells. In addition, AEE could reverse the action of PPARγ inhibitor T0070907 and/or ox-LDL. Therefore, AEE may become an effective candidate drug for the prevention of atherosclerosis.

Multi-omics reveals aspirin eugenol ester alleviates neurological disease.

BACKGROUND: Exosomes play an essential role in maintaining normal brain function due to their ability to cross the blood-brain barrier. Aspirin eugenol ester (AEE) is a new medicinal compound synthesized by the esterification of aspirin with eugenol using the prodrug principle. Aspirin has been reported to have neuroprotective effects and may be effective against neurodegenerative diseases. PURPOSE: This study wanted to investigate how AEE affected neurological diseases in vivo and in vitro. EXPERIMENTAL APPROACH: A multi-omics approach was used to explore the effects of AEE on the nervous system. Gene and protein expression changes of BDNF and NEFM in SY5Y cells after AEE treatment were detected using RT-qPCR and Western Blot. KEY RESULTS: The multi-omics results showed that AEE could regulate neuronal synapses, neuronal axons, neuronal migration, and neuropeptide signaling by affecting transport, inflammatory response, and regulating apoptosis. Exosomes secreted by AEE-treated Caco-2 cells could promote the growth of neurofilaments in SY5Y cells and increased the expression of BDNF and NEFM proteins in SY5Y cells. miRNAs in the exosomes of AEE-treated Caco-2 cells may play an important role in the activation of SY5Y neuronal cells. CONCLUSIONS: In conclusion, AEE could play positive effects on neurological-related diseases.

Long-term Intensive Soccer Training Induced Dynamic Reconfiguration of Brain Network.

Long-term motor skill learning has been shown to impact the functional plasticity of the brain. Athletes, as a unique population, exhibit remarkable adaptive changes in the static properties of their brain networks. However, studying the differences between expert and novice athletes using a dynamic brain network framework can provide a fresh perspective on how motor skill learning affects the functional organization of the brain. In this study, we investigated the dynamic properties of brain networks in expert and novice soccer players at the whole-brain, network, and region-based levels. Our findings revealed that expert soccer players displayed reduced integration and increased segregation at the whole-brain level. As for network level, experts exhibited increased segregation and reduced flexibility in the visual network, enhanced integration between the visual and ventral attention networks, and decreased integration in the subcortical-sensorimotor and subcortical-cerebellar networks. Additionally, specific brain regions within the visual network exhibited greater recruitment in expert soccer players compared to novices at the nodal level. Furthermore, classification analyses demonstrated the critical role played by the visual network in the classification process. In conclusion, our study provides new insights into the dynamic properties of brain networks in expert and novice soccer players, and suggests that reduced integration and increased segregation in the visual network may be neuroimaging marker that distinguish expert soccer players from novices. Our findings may have implications for the training and development of athletes and advance our understanding of how motor skill learning affects brain functional organization.

The Transport and Uptake of Resveratrol Mediated via Glucose Transporter 1 and Its Antioxidant Effect in Caco-2 Cells.

Resveratrol has anti-inflammatory, anti-cancer, and anti-aging pharmacological activities. There is currently a gap in academic research regarding the uptake, transport, and reduction of H2O2-induced oxidative damage of resveratrol in the Caco-2 cell model. This study investigated the role of resveratrol in the uptake, transport, and alleviation of H2O2-induced oxidative damage in Caco-2 cells. In the Caco-2 cell transport model, it was observed that the uptake and transport of resveratrol (10, 20, 40, and 80 µM) were time dependent and concentration dependent. Different temperatures (37 °C vs. 4 °C) could significantly affect the uptake and transportation of resveratrol. The apical to basolateral transport of resveratrol was markedly reduced by STF-31, a GLUT1 inhibitor, and siRNA intervention. Furthermore, resveratrol pretreatment (80 µM) improves the viability of Caco-2 cells induced by H2O2. In a cellular metabolite analysis combined with ultra-high performance liquid chromatography-tandem mass spectrometry, 21 metabolites were identified as differentials. These differential metabolites belong to the urea cycle, arginine and proline metabolism, glycine and serine metabolism, ammonia recycling, aspartate metabolism, glutathione metabolism, and other metabolic pathways. The transport, uptake, and metabolism of resveratrol suggest that oral resveratrol could prevent intestinal diseases caused by oxidative stress.

Improved Luminous Efficiency of AgInS2 Quantum Dots and Zeolitic Imidazolate Framework-70 Composite for White Light Emitting Diode Applications.

The application of multiple quantum dots (QDs) in the field of white light emitting diodes (WLEDs) is still an important challenge due to their low luminous efficiency and quenching phenomenon. In this paper, we prepared AgInS2 QDs/zeolitic imidazolate framework-70 (AIS/ZIF-70) composite by a microwave hydrothermal method. Owing to the high porosity and stability of ZIF-70, it could effectively prevent quenching issues due to the aggregation of QDs. Since the ZIF-70 and QDs were chemically bonded, the formation of the ZnS layer could effectively passivate the surface defect and thus the quantum yield reached 21.49 % in aqueous solution. The luminous efficiency (LE) of the assembled AIS/ZIF-based WLED was reinforced by 6.8 times with a molar ratio of AgIn/Zn=18, i. e. at 5.26 % molar fraction of ZIF-70. Moreover, the color rendering index (CRI) and correlated color temperature (CCT) of AIS/ZIF-based WLED were 84.3 and 3631 K, respectively, indicating its potential application in solid-state lighting.

Development and validation of a novel non-invasive test for diagnosing nonalcoholic fatty liver disease in Chinese children.

BACKGROUND: With the exploding prevalence of obesity, many children are at risk of developing nonalcoholic fatty liver disease. Using anthropometric and laboratory parameters, our study aimed to develop a model to quantitatively evaluate liver fat content (LFC) in children with obesity. METHODS: A well-characterized cohort of 181 children between 5 and 16 years of age were recruited to the study in the Endocrinology Department as the derivation cohort. The external validation cohort comprised 77 children. The assessment of liver fat content was performed using proton magnetic resonance spectroscopy. Anthropometry and laboratory metrics were measured in all subjects. B-ultrasound examination was carried out in the external validation cohort. The Kruskal-Wallis test, Spearman bivariate correlation analyses, univariable linear regressions and multivariable linear regression were used to build the optimal predictive model. RESULTS: The model was based on indicators including alanine aminotransferase, homeostasis model assessment of insulin resistance, triglycerides, waist circumference and Tanner stage. The adjusted R2 of the model was 0.589, which presented high sensitivity and specificity both in internal [sensitivity of 0.824, specificity of 0.900, area under curve (AUC) of 0.900 with a 95% confidence interval: 0.783-1.000] and external validation (sensitivity of 0.918 and specificity of 0.821, AUC of 0.901 with a 95% confidence interval: 0.818-0.984). CONCLUSIONS: Our model based on five clinical indicators was simple, non-invasive, and inexpensive; it had high sensitivity and specificity in predicting LFC in children. Thus, it may be useful for identifying children with obesity who are at risk for developing nonalcoholic fatty liver disease.