Structural and histochemical aspects of the Gymnotus carapo tegument (Teleostei: Gymnotiformes).

The present study aims to structurally and histochemically characterize the Gymnotus carapo tegument. 30 specimens were captured and slaughtered by spinal section with anesthesia. The observation was carried out with a stereoscopic microscope and the body surface was photographed. Fragments of the dorsal, ventral and lateral region were fixed in Bouin's solution for 12 hours and subsequently preserved in 70% alcohol. They were subsequently observed in the scanning electron microscope (SEM). The preparation for SEM was performed following the standardized protocol. Histological preparations were made, and the cuts were colored with H-E, PAS and Coomassie Blue. The images were obtained in an Olympus BX41-ENUTV-4 microscope. From the observations in SEM a plain tegument with pores of different sizes could be evidenced. The scales of the different regions of the body have different ornaments. Microscopically it was composed of a stratified non-keratinized epithelium consisting of two types of morphologically distinct cells: epidermal cells and mucous cells (PAS-Commassie Blue positive). Under the epithelium there is a layer of dense irregular connective tissue with associated chromatophores and more deeply scales. These analyzes are the basis for future studies that will focus on elucidating the events related to integumentary healing in this species.

Hepatic and gastroprotective activity of Serjania marginata leaf aqueous extract in Nile tilapia (Oreochromis niloticus).

The aim of this study was to assess the leaf aqueous extract composition of Serjania marginata and the effects of its inclusion on the diet of Nile tilapia (Oreochromis niloticus), with respect to the activity of digestive enzymes and enzymes associated with the metabolism of the liver and intestine and liver histopathology. Fish (initial mean weight, 54.36 ± 17.04 g) were divided into groups: fasting (without feeding), control (commercial feed), and treatment (commercial feed with leaf aqueous extract of Serjania marginata), and in each aquarium, there were five individuals and the fish fed ad libitum for 15 days. Treatment fish had ingested on average 224.3 mg of extract/kg of fish/day. In the extract analysis by mass spectrometry, quercitrin, isoquercitrin, A-type proanthocyanidin trimer, and quinic acid were identified. In the enzymatic activity, fish from the treatment group showed higher level of alkaline phosphatase, while the hepatotoxic markers (AST and ALT) and levels of lipase, amylase, and nonspecific protease did not differ (p > 0.05). In liver histopathological analysis, it was observed that fish from the treatment showed normal structure, while abnormalities were associated with control (fibrosis, loss of cordonal architecture, vacuolated hepatocytes with nucleus displaced to the periphery) and fasting (reduction in hepatocyte size and sinusoidal space). The intestine histopathology evidenced that the extract favored the development of goblet cells and intestinal fold height. The results indicated that the leaf aqueous extract of S. marginata assists in the structural maintenance of the liver and intestine and stimulates intestinal alkaline phosphatase production in Nile tilapia, suggesting that the identified compounds act on the liver and intestine, showing hepatoprotective effects and stimulating intestinal digestion.

Disaster Governance for Community Resilience in Coastal Towns: Chilean Case Studies.

This study aimed to further our understanding of a characteristic of Community Resilience known as Disaster Governance. Three attributes of Disaster Governance-redundancy, diversity, and overlap-were studied in four coastal towns in southern Chile that are at risk of tsunamis. Overall, we explored how different spatial structures of human settlements influence Disaster Governance. Using the Projective Mapping Technique, the distribution of emergency institutions (N = 32) and uses given to specific sites (e.g., for refuge, sanitary purposes and medical attention) were mapped. Content and GIS analyses (Directional Distribution and Kernel Density Index) were used to explore the dispersion and concentration of institutions and uses in each town. Disaster Governance was found to be highly influenced by decisions taken during regional, urban, and emergency planning. Governance is better in towns of higher order in the communal hierarchical structure. Most of the emergency institutions were found to be located in central and urban areas, which, in turn, assures more redundancy, overlap, and diversity in governance in the event of a tsunami. Lack of flexibility of emergency plans also limits governance in rural and indigenous areas. While the spatial relationships found in this study indicate that urban sectors have better Disaster Governance than rural and indigenous sectors, the influence of resource availability after tsunamis, the role and responsibility of different levels of governments, and the politics of disaster also play an important role in Disaster Governance for determining Community Resilience. These findings shed light on emergency planning and aspects of the Disaster Management cycle.

Jatropha cake (Jatropha curcas): hepatotoxic implications / Torta de pinhão-manso (Jatropha curcas): implicações hepatotóxicas

Jatropha has been highlighted as an oleaginous potential for the production of biofuel. The cake, produced by oil extraction, could be used in animal feed. However, some varieties of jatropha are toxic by limiting their incorporation into animal diets. The objective of this study was to evaluate the hepatotoxicity of diets added with jatropha cake ­ JC (Jatropha curcas) in rats. Thirty-five (35) male Wistar adults rats (Rattus norvegicus) with initial weight of 352.1 ± 26.8 g were used. The animals were fed for 21 days with the diets: control, 10, 25, 40 and 50% JC. In the feeding with 50% JC the animals presented themselves prostrate and with piloerection. Development and survival decreased, since the inclusion of JC in diets increased. In rats submitted to 10 and 25% JC there was an increase of 17.52% in the hepatosomatic index in relation to the control group. Increase of JC in the rat diet promoted an increase in the activity of ALT and AST enzymes. Anatomic-histopathological evaluation demonstrated that, regardless of the levels tested, JC in rat diet causes hypertrophy of the hepatocytes, with a reduction in energy reserves. This study demonstrated that the use of JC resulted in decreased food intake, associated with weight loss due to the clinical pattern of toxicity, demonstrated by biochemical and histopathological changes in the liver. It was concluded that the inclusion of jatropha cake in rat feeding presents high hepatotoxic potential leading to lesions in the liver parenchyma.(AU)

O pinhão-manso tem se destacado como oleaginosa potencial para a produção de biocombustível. A torta, coproduto da extração do óleo, poderia ser utilizada na alimentação animal. No entanto, algumas variedades de pinhão-manso são tóxicas, limitando sua incorporação em dietas animais. Objetivou-se neste estudo avaliar a hepatotoxicidade de dietas acrescidas de torta de pinhão-manso (Jatropha curcas) em ratos. Foram utilizados trinta e cinco (35) ratos Wistar (Rattus norvergicus) machos adultos com peso inicial de 352,1 ± 26,8 g. Os animais foram alimentados por 21 dias com as dietas: controle, 10, 25, 40 e 50% TPM. Na alimentação com 50% TPM os animais apresentaram-se prostrados e com piloereção. O desenvolvimento e a sobrevivência apresentaram diminuição conforme o aumento da inclusão de TPM nas dietas. Em ratos submetidos a 10 e 25% TPM houve aumento de 17,52% no índice hepatossomático em relação ao grupo controle. O aumento de TPM na dieta de ratos promoveu aumento da atividade das enzimas ALT e AST. A avaliação anatomo-histopatológica revelou que, independentemente dos níveis testados, a TPM na alimentação de ratos provoca hipertrofia dos hepatócitos, com redução das reservas energéticas. Este estudo demonstrou que a utilização de TPM resultou em diminuição do consumo de alimento associado à perda de peso devido ao quadro clínico de toxicidade demonstrado pelas alterações bioquímica e histopatológica no fígado. Conclui-se que a inclusão de torta de pinhão-manso na alimentação de ratos apresenta alto potencial hepatotóxico levando a lesões no parênquima hepático.(AU)

Jatropha cake (Jatropha curcas): hepatotoxic implications / Torta de pinhão-manso (Jatropha curcas): implicações hepatotóxicas

Jatropha has been highlighted as an oleaginous potential for the production of biofuel. The cake, produced by oil extraction, could be used in animal feed. However, some varieties of jatropha are toxic by limiting their incorporation into animal diets. The objective of this study was to evaluate the hepatotoxicity of diets added with jatropha cake ­ JC (Jatropha curcas) in rats. Thirty-five (35) male Wistar adults rats (Rattus norvegicus) with initial weight of 352.1 ± 26.8 g were used. The animals were fed for 21 days with the diets: control, 10, 25, 40 and 50% JC. In the feeding with 50% JC the animals presented themselves prostrate and with piloerection. Development and survival decreased, since the inclusion of JC in diets increased. In rats submitted to 10 and 25% JC there was an increase of 17.52% in the hepatosomatic index in relation to the control group. Increase of JC in the rat diet promoted an increase in the activity of ALT and AST enzymes. Anatomic-histopathological evaluation demonstrated that, regardless of the levels tested, JC in rat diet causes hypertrophy of the hepatocytes, with a reduction in energy reserves. This study demonstrated that the use of JC resulted in decreased food intake, associated with weight loss due to the clinical pattern of toxicity, demonstrated by biochemical and histopathological changes in the liver. It was concluded that the inclusion of jatropha cake in rat feeding presents high hepatotoxic potential leading to lesions in the liver parenchyma.(AU)

O pinhão-manso tem se destacado como oleaginosa potencial para a produção de biocombustível. A torta, coproduto da extração do óleo, poderia ser utilizada na alimentação animal. No entanto, algumas variedades de pinhão-manso são tóxicas, limitando sua incorporação em dietas animais. Objetivou-se neste estudo avaliar a hepatotoxicidade de dietas acrescidas de torta de pinhão-manso (Jatropha curcas) em ratos. Foram utilizados trinta e cinco (35) ratos Wistar (Rattus norvergicus) machos adultos com peso inicial de 352,1 ± 26,8 g. Os animais foram alimentados por 21 dias com as dietas: controle, 10, 25, 40 e 50% TPM. Na alimentação com 50% TPM os animais apresentaram-se prostrados e com piloereção. O desenvolvimento e a sobrevivência apresentaram diminuição conforme o aumento da inclusão de TPM nas dietas. Em ratos submetidos a 10 e 25% TPM houve aumento de 17,52% no índice hepatossomático em relação ao grupo controle. O aumento de TPM na dieta de ratos promoveu aumento da atividade das enzimas ALT e AST. A avaliação anatomo-histopatológica revelou que, independentemente dos níveis testados, a TPM na alimentação de ratos provoca hipertrofia dos hepatócitos, com redução das reservas energéticas. Este estudo demonstrou que a utilização de TPM resultou em diminuição do consumo de alimento associado à perda de peso devido ao quadro clínico de toxicidade demonstrado pelas alterações bioquímica e histopatológica no fígado. Conclui-se que a inclusão de torta de pinhão-manso na alimentação de ratos apresenta alto potencial hepatotóxico levando a lesões no parênquima hepático.(AU)

Levels of digestible protein to surubim (Pseudoplatystoma sp) reared in net cages / Níveis de proteína digestível para surubim (Pseudoplatystoma sp) criados em tanque-rede

The Pseudoplatystoma sp. is species of carnivorous fish that require special attention in the diet offered. This work had the objective to determine the digestible protein requirement of juvenile the Pseudoplatystoma sp. reared in net cages. The test consisted of four isoenergetic diets (2606.69 ± 39.16 kcal kg-1 of digestible energy) containing increasing levels of digestible protein (23, 24, 26 and 28%PD) provided to juveniles of surubim (157.35±11.23g) for five months. The parameters of growth, fillet composition, metabolic liver enzymes and morphometry of the intestine and liver were analyzed in completely randomized design with four treatments and four replicates. An increase of protein in the diet provided better weight gain. The metabolic liver enzymes increased in fish fed 24PD. The histopathological changes were not observed in the liver of the fish. The bowel histology showed adaptation to increased protein in the diet until the 26 level PD. juveniles of Pseudoplatystoma sp. Were demanding in digestible protein, showing the best results of production performance and nutrient use efficiency with the diet containing 28%PD.(AU)

O surubim (Pseudoplatystoma sp.) é uma das espécie de peixe carnívora que exige atenção especial na dieta ofertada. Este trabalho teve o objetivo determinar a exigência de proteína digestível de juvenis de surubim (Pseudoplatystoma sp.) criados em tanque-rede. O ensaio foi constituído por quatro dietas isoenergéticas (2606,69±39,16 kcal. kg-1 de energia digestível) contendo níveis crescentes de proteína digestível (23, 24, 26 e 28 % PD) fornecidas aos juvenis de surubim (157,35± 11,23g) durante cinco meses. Os parâmetros de crescimentos, composição do filé, enzimas metabólicas hepáticas e morfometria do intestino e fígado foram analisados em delineamento inteiramente casualizado com quatro tratamentos e quatro repetições. O aumento de proteína na dieta proporcionou melhor ganho em peso. As enzimas do metabolismo hepático aumentaram nos peixes alimentados com 24%PD. Não foram observadas alterações histopatológicas no fígado dos peixes. A histologia do intestino revelou adaptação ao aumento de proteína na dieta até o nível de 26% PD. Os juvenis de surubim mostraram-se exigentes em proteína digestível, apresentando os melhores resultados de desempenho de produção e de eficiência de utilização dos nutrientes com a dieta contendo 28% de PD.(AU)

Levels of digestible protein to surubim (Pseudoplatystoma sp) reared in net cages / Níveis de proteína digestível para surubim (Pseudoplatystoma sp) criados em tanque-rede

The Pseudoplatystoma sp. is species of carnivorous fish that require special attention in the diet offered. This work had the objective to determine the digestible protein requirement of juvenile the Pseudoplatystoma sp. reared in net cages. The test consisted of four isoenergetic diets (2606.69 ± 39.16 kcal kg-1 of digestible energy) containing increasing levels of digestible protein (23, 24, 26 and 28%PD) provided to juveniles of surubim (157.35±11.23g) for five months. The parameters of growth, fillet composition, metabolic liver enzymes and morphometry of the intestine and liver were analyzed in completely randomized design with four treatments and four replicates. An increase of protein in the diet provided better weight gain. The metabolic liver enzymes increased in fish fed 24PD. The histopathological changes were not observed in the liver of the fish. The bowel histology showed adaptation to increased protein in the diet until the 26 level PD. juveniles of Pseudoplatystoma sp. Were demanding in digestible protein, showing the best results of production performance and nutrient use efficiency with the diet containing 28%PD.

O surubim (Pseudoplatystoma sp.) é uma das espécie de peixe carnívora que exige atenção especial na dieta ofertada. Este trabalho teve o objetivo determinar a exigência de proteína digestível de juvenis de surubim (Pseudoplatystoma sp.) criados em tanque-rede. O ensaio foi constituído por quatro dietas isoenergéticas (2606,69±39,16 kcal. kg-1 de energia digestível) contendo níveis crescentes de proteína digestível (23, 24, 26 e 28 % PD) fornecidas aos juvenis de surubim (157,35± 11,23g) durante cinco meses. Os parâmetros de crescimentos, composição do filé, enzimas metabólicas hepáticas e morfometria do intestino e fígado foram analisados em delineamento inteiramente casualizado com quatro tratamentos e quatro repetições. O aumento de proteína na dieta proporcionou melhor ganho em peso. As enzimas do metabolismo hepático aumentaram nos peixes alimentados com 24%PD. Não foram observadas alterações histopatológicas no fígado dos peixes. A histologia do intestino revelou adaptação ao aumento de proteína na dieta até o nível de 26% PD. Os juvenis de surubim mostraram-se exigentes em proteína digestível, apresentando os melhores resultados de desempenho de produção e de eficiência de utilização dos nutrientes com a dieta contendo 28% de PD.

[Frequency of E. vermicularis in a rural school population of Quipile, Colombia, 2001]. / Frecuencia de E. vermicularis en una población escolar rural de Quipile, Colombia, 2001.

OBJECTIVES: To study the prevalence of Enterobius vermicularis infection in children aged 3 to 14 years in a rural zone of the town of Quipile, Colombia. METHODS: 159 children from 14 villages, with a homogeneous distribution according to their degree of scholarity, were studied. The detection of Enterobius vermicularis eggs was done by the Graham method. The design was a cross-sectional descriptive study, for which a convenience non-probabilistic sampling was carried out. Fisher's exact test and chi 2 were performed for data analysis. Multivariate analysis by multiple correspondence was also carried out. RESULTS: 2.5% of the children were found to be infected with E. vermicularis and 5% with Ascaris lumbricoides. This frequency is low when compared to reports from other authors; one of the possible causes is the sampling method used. CONCLUSIONS: Deficient sanitation conditions of the population studied as well as the low frequency of anal hygiene practices may represent behavioural risk factors which favour the transmission of this helminth.

Hematological and histopathological alteration in pacu Piaractus mesopotamicus Holmberg, 1887 (Osteichthyes, Characidae) after treatment with copper sulphate (CuSO4) / Alterações hematológicas e histopatológicas em pacu, Piaractus mesopotamicus Holmberg, 1887 (Osteichthyes, Characidae),

This paper evaluated the haematological and glycaemic parameters in Piaractus mesopotamicus (Osteichthyes, Characidae) infected with Monogenea Anacanthorus penilabiatus Boeger, Husak & Martins, 1995 (Dactylogyridae) after treatment with 0.50 mg/L and 1.00 mg/L of copper sulphate (CuSO4). The efficacy of the CuSO4 was observed in the first day after administration but not after eight, fifteen or thirty days. The histopathological analyses showed hyperplasia of the epithelium and circulatory changes in the gills. In the first day after treatment significant changes (P

Este trabalho avaliou os valores hematológicos e glicêmicos e histopatologia de Piaractus mesopotamicus (Osteichthyes, Characidae) infectados com Monogenea Anacanthorus penilabiatus Boeger, Husak & Martins, 1995 (Dactylogyridae) após tratamento com 0,50 mg/L e 1,00 mg/L de sulfato de cobre (CuSO4). A eficiência da droga sobre as infecções com Monogenea foi observada no primeiro dia após administração mas não após 8, 15 ou 30 dias do tratamento. As análises histopatológicas mostraram hiperplasia do epitélio e alterações circulatórias nas brânquias. No primeiro dia após tratamento, foram observadas alterações significativas (P

Hematological and histopathological alteration in pacu Piaractus mesopotamicus Holmberg, 1887 (Osteichthyes, Characidae) after treatment with copper sulphate (CuSO4) / Alterações hematológicas e histopatológicas em pacu, Piaractus mesopotamicus Holmberg, 1887 (Osteichthyes, Characidae),

This paper evaluated the haematological and glycaemic parameters in Piaractus mesopotamicus (Osteichthyes, Characidae) infected with Monogenea Anacanthorus penilabiatus Boeger, Husak & Martins, 1995 (Dactylogyridae) after treatment with 0.50 mg/L and 1.00 mg/L of copper sulphate (CuSO4). The efficacy of the CuSO4 was observed in the first day after administration but not after eight, fifteen or thirty days. The histopathological analyses showed hyperplasia of the epithelium and circulatory changes in the gills. In the first day after treatment significant changes (P

Este trabalho avaliou os valores hematológicos e glicêmicos e histopatologia de Piaractus mesopotamicus (Osteichthyes, Characidae) infectados com Monogenea Anacanthorus penilabiatus Boeger, Husak & Martins, 1995 (Dactylogyridae) após tratamento com 0,50 mg/L e 1,00 mg/L de sulfato de cobre (CuSO4). A eficiência da droga sobre as infecções com Monogenea foi observada no primeiro dia após administração mas não após 8, 15 ou 30 dias do tratamento. As análises histopatológicas mostraram hiperplasia do epitélio e alterações circulatórias nas brânquias. No primeiro dia após tratamento, foram observadas alterações significativas (P