Temporal dynamics of muscle optical properties during degeneration and regeneration in a canine muscle xenograft model.

We studied time-dependent changes in muscle optical properties during degeneration and regeneration using polarization-sensitive optical coherence tomography (PSOCT). Excised canine muscle transplants in a xenograft mouse model were imaged ex vivo from 3- to 112-day post-transplantation. PSOCT images were quantified to evaluate post-transplantation changes of three optical/structural properties: attenuation, birefringence and fiber alignment. The birefringence and fiber alignment decreased after transplantation until 20∼30-day and recovered thereafter. The attenuation coefficient showed a reversed trend over the same period of time. These results suggest that optical properties could be used for monitoring skeletal muscle degeneration and regeneration.

Simultaneous tractography and elastography imaging of the zone-specific structural and mechanical responses in articular cartilage under compressive loading.

We quantified the precise zonal cartilage structural and mechanical responses to unconfined compressive loading by using simultaneous PSOCT based optical tractography and elastography imaging. Twelve bovine knee articular cartilage samples from six animals were imaged under bulk compression from 4% to 20%. The results revealed strong evidence that the conventional radial zone could be divided into two sub-zones with distinct mechanical properties. The "upper" part of the radial zone played a critical role in "absorbing" the mechanical compression. The study also showed that the zonal fiber organization greatly affected the cartilage structural and mechanical responses. A strong correlation was observed between the optical birefringence and logarithm of the Young's modulus. These new results provide useful information for improving mechanical modeling of articular cartilage and developing better cartilage-mimetic biomaterials.

Measurement of biaxial optical birefringence in articular cartilage.

Optical birefringence is a valuable parameter in evaluating collagen organization and assessing collagen degeneration in articular cartilage. A uniaxial birefringent model is implied when using existing methods such as polarized light microscopy in characterizing birefringence in cartilage. However, some studies suggest the existence of a sheet-like collagen organization in articular cartilage, which requires a biaxial birefringence model to describe. In this study, we applied a multi-incident birefringence measurement procedure to investigate the biaxial birefringence in articular cartilage. The results supported the existence of a small yet significant biaxial birefringence effect, which was in agreement with the expectation from a sheet-like collagen organization.

Automatic quantification of microscopic heart damage in a mouse model of Duchenne muscular dystrophy using optical polarization tractography.

Quantification of microscopic myocardium damage in a diseased heart is important in studying disease progression and evaluating treatment outcome. However, it is challenging to use traditional histology and existing medical imaging modalities to quantify all microscopic damages in a small animal heart. Here, a method was developed for fast visualization and quantification of focal tissue damage in the mouse heart based on the fiber alignment index of the local myofiber organization obtained in optical polarization tractography (OPT). This method was tested in freshly excised hearts of the mdx4cv mouse, a commonly used mouse model for studying Duchenne cardiomyopathy. The hearts of age-matched C57BL/6 mice were also imaged as the normal controls. The results revealed a significant amount of damage in the mdx4cv hearts. Histology comparisons confirmed the damage identified by OPT. This fast and automatic method may greatly enhance preclinical studies in murine models of heart diseases.

Parametric imaging of collagen structural changes in human osteoarthritic cartilage using optical polarization tractography.

Collagen degeneration is an important pathological feature of osteoarthritis. The purpose of this study is to investigate whether the polarization-sensitive optical coherence tomography (PSOCT)-based optical polarization tractography (OPT) can be useful in imaging collagen structural changes in human osteoarthritic cartilage samples. OPT eliminated the banding artifacts in conventional PSOCT by calculating the depth-resolved local birefringence and fiber orientation. A close comparison between OPT and PSOCT showed that OPT provided improved visualization and characterization of the zonal structure in human cartilage. Experimental results obtained in this study also underlined the importance of knowing the collagen fiber orientation in conventional polarized light microscopy assessment. In addition, parametric OPT imaging was achieved by quantifying the surface roughness, birefringence, and fiber dispersion in the superficial zone of the cartilage. These quantitative parametric images provided complementary information on the structural changes in cartilage, which can be useful for a comprehensive evaluation of collagen damage in osteoarthritic cartilage.

High resolution imaging of the fibrous microstructure in bovine common carotid artery using optical polarization tractography.

The biomechanical properties of artery are primarily determined by the fibrous structures in the vessel wall. Many vascular diseases are associated with alternations in the orientation and alignment of the fibrous structure in the arterial wall. Knowledge on the structural features of the artery wall is crucial to our understanding of the biology of vascular diseases and the development of novel therapies. Optical coherence tomography (OCT) and polarization-sensitive OCT have shown great promise in imaging blood vessels due to their high resolution, fast acquisition, good imaging depth, and large field of view. However, the feasibility of using OCT based methods for imaging fiber orientation and distribution in the arterial wall has not been investigated. Here we show that the optical polarization tractography (OPT), a technology developed from Jones matrix OCT, can reveal the fiber orientation and alignment in the bovine common carotid artery. The fiber orientation and alignment data obtained in OPT provided a robust contrast marker to clearly resolve the intima and media boundary of the carotid artery wall. Optical polarization tractography can visualize fiber orientation and alignment in carotid artery.

Nondestructive imaging of fiber structure in articular cartilage using optical polarization tractography.

Collagen fiber orientation plays an important role in determining the structure and function of the articular cartilage. However, there is currently a lack of nondestructive means to image the fiber orientation from the cartilage surface. The purpose of this study is to investigate whether the newly developed optical polarization tractography (OPT) can image fiber structure in articular cartilage. OPT was applied to obtain the depth-dependent fiber orientation in fresh articular cartilage samples obtained from porcine phalanges. For comparison, we also obtained collagen fiber orientation in the superficial zone of the cartilage using the established split-line method. The direction of each split-line was quantified using image processing. The orientation measured in OPT agreed well with those obtained from the split-line method. The correlation analysis of a total of 112 split-lines showed a greater than 0.9 coefficient of determination (R2) between the split-line results and OPT measurements obtained between 40 and 108???m in depth. In addition, the thickness of the superficial layer can also be assessed from the birefringence images obtained in OPT. These results support that OPT provides a nondestructive way to image the collagen fiber structure in articular cartilage. This technology may be valuable for both basic cartilage research and clinical orthopedic applications.