Redox-regulation of mitochondrial metabolism through thioredoxin o1 facilitates light induction of photosynthesis.

Despite the well-known biochemistry of the major pathways involved in central carbon and amino acid metabolism, there are still gaps regarding their regulation or regulatory interactions. Recent research demonstrated the physiological significance of the mitochondrial redox machinery, particularly thioredoxin o1 (TRXo1), for proper regulation of the tricarboxylic acid cycle, components of the mitochondrial electron transport chain and photorespiration. These findings imply that TRXo1 regulation contributes to the metabolic acclimation toward changes in the prevailing environmental conditions. Here, we analyzed if TRXo1 is involved in the light induction of photosynthesis. Our results show that the trxo1 mutant activates CO2 assimilation rates to a significantly lower extend than wild type in response to short-term light/dark changes. Metabolite analysis suggests that activation of glycine-to-serine conversion catalyzed through glycine decarboxylase in conjunction with serine hydroxymethyltransferase in trxo1 is slowed down at onset of illumination. We propose that redox regulation via TRXo1 is necessary to allow the rapid induction of mitochondrial steps of the photorespiratory cycle and, in turn, to facilitate light-induction of photosynthesis.

Redox-Regulation of Photorespiration through Mitochondrial Thioredoxin o1.

Photorespiration sustains photosynthesis in the presence of oxygen due to rapid metabolization of 2-phosphoglycolate, the major side-product of the oxygenase activity of Rubisco that also directly impedes carbon assimilation and allocation. Despite the fact that both the biochemical reactions and the underlying genetics are well characterized, information concerning the regulatory mechanisms that adjust photorespiratory flux is rare. Here, we studied the impact of mitochondrial-localized thioredoxin o1 (TRXo1) on photorespiratory metabolism. The characterization of an Arabidopsis (Arabidopsis thaliana) transfer DNA insertional line (trxo1-1) revealed an increase in the stoichiometry of photorespiratory CO2 release and impaired Gly-to-Ser turnover after a shift from high-to-low CO2 without changes in Gly decarboxylase (GDC) gene or protein expression. These effects were distinctly pronounced in a double mutant, where the TRXo1 mutation was combined with strongly reduced GDC T-protein expression. The double mutant (TxGT) showed reduced growth in air but not in high CO2, decreased photosynthesis, and up to 54-fold more Gly alongside several redox-stress-related metabolites. Given that GDC proteins are potential targets for redox-regulation, we also examined the in vitro properties of recombinant GDC l-proteins (lipoamide dehydrogenase) from plants and the cyanobacterium Synechocystis species strain PCC6803 and observed a redox-dependent inhibition by either artificial reducing agents or TRXo1 itself. Collectively, our results demonstrate that TRXo1 potentially adjusts photorespiration via redox-regulation of GDC in response to environmental changes.