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1.
Acta Cir Bras ; 39: e392824, 2024.
Article in English | MEDLINE | ID: mdl-39046039

ABSTRACT

PURPOSE: to evaluate biocompatibility and osteogenic potential of hydroxyapatite/alginate composite after its implantation on rat calvarian critical bone defect. METHODS: thirty adults male Wistar rats were randomly distributed into two groups: GHA - critical bone defect filled with hydroxyapatite/alginate composite granules (HA/Alg) and CG - critical bone defect without biomaterial; evaluated at biological points of 15, 45 and 120 days. RESULTS: the histomorphometrically analyses for GHA showed osteoid matrix deposition (OM) among the granules and towards the center of the defect in centripetal direction throughout the study, with evident new bone formation at 120 days, resulting in filling 4/5 of the initial bone defect. For CG, this finding was restricted to the edges of the bone margins and formation of connective tissue on the residual area was found in all biological points. Inflammatory response on GHA was chronic granulomatous type, discrete and regressive for all biological points. Throughout the study, the CG presented mononuclear inflammatory infiltrate diffuse and regressive. Histomorphometry analyses showed that OM percentage was evident for GHA group when compared to CG group in all analyzed periods (p > 0.05). CONCLUSIONS: the biomaterial evaluated at this study showed to be biocompatible, bioactive, osteoconductive and biodegradable synchronously with bone formation.


Subject(s)
Alginates , Biocompatible Materials , Bone Regeneration , Bone Substitutes , Durapatite , Materials Testing , Rats, Wistar , Animals , Male , Bone Regeneration/drug effects , Bone Regeneration/physiology , Alginates/pharmacology , Durapatite/pharmacology , Durapatite/therapeutic use , Biocompatible Materials/therapeutic use , Bone Substitutes/therapeutic use , Random Allocation , Osteogenesis/drug effects , Osteogenesis/physiology , Hexuronic Acids/pharmacology , Glucuronic Acid/pharmacology , Skull/surgery , Skull/drug effects , Time Factors , Rats , Reproducibility of Results
2.
Braz Dent J ; 35: e245461, 2024.
Article in English | MEDLINE | ID: mdl-38775590

ABSTRACT

This study aimed to evaluate the osteogenic potential of hydroxyapatite (HA), Alginate (Alg), and Gelatine (Gel) composite in a critical-size defect model in rats. Twenty-four male rats were divided into three groups: a negative control with no treatment (Control group), a positive control treated with deproteinized bovine bone mineral (DBBM group), and the experimental group treated with the new HA-Alg-Gel composite (HA-Alg-Gel group). A critical size defect (8.5mm) was made in the rat's calvaria, and the bone formation was evaluated by in vivo microcomputed tomography analysis (µCT) after 1, 15, 45, and 90 days. After 90 days, the animals were euthanized and histological and histomorphometric analyses were performed. A higher proportion of mineralized tissue/biomaterial was observed in the DBBM group when compared to the HA-Alg-Gel and Control groups in the µCT analysis during all analysis periods. However, no differences were observed in the mineralized tissue/biomaterial proportion observed on day 1 (immediate postoperative) in comparison to later periods of analysis in all groups. In the histomorphometric analysis, the HA-Alg-Gel and Control groups showed higher bone formation than the DBBM group. Moreover, in histological analysis, five samples of the HA-Alg-Gal group exhibited formed bone spicules adjacent to the graft granules against only two of eight samples in the DBBM group. Both graft materials ensured the maintenance of defect bone thickness, while a tissue thickness reduction was observed in the control group. In conclusion, this study demonstrated the osteoconductive potential of HA-Alg-Gel bone graft by supporting new bone formation around its particles.


Subject(s)
Alginates , Bone Regeneration , Durapatite , Gelatin , Skull , X-Ray Microtomography , Animals , Bone Regeneration/drug effects , Durapatite/pharmacology , Skull/surgery , Skull/diagnostic imaging , Rats , Male , Biocompatible Materials , Glucuronic Acid , Rats, Wistar , Hexuronic Acids , Osteogenesis/drug effects , Bone Substitutes
3.
Braz. dent. j ; Braz. dent. j;35: e24, 2024. graf
Article in English | LILACS-Express | LILACS, BBO - Dentistry | ID: biblio-1557215

ABSTRACT

Abstract This study aimed to evaluate the osteogenic potential of hydroxyapatite (HA), Alginate (Alg), and Gelatine (Gel) composite in a critical-size defect model in rats. Twenty-four male rats were divided into three groups: a negative control with no treatment (Control group), a positive control treated with deproteinized bovine bone mineral (DBBM group), and the experimental group treated with the new HA-Alg-Gel composite (HA-Alg-Gel group). A critical size defect (8.5mm) was made in the rat's calvaria, and the bone formation was evaluated by in vivo microcomputed tomography analysis (µCT) after 1, 15, 45, and 90 days. After 90 days, the animals were euthanized and histological and histomorphometric analyses were performed. A higher proportion of mineralized tissue/biomaterial was observed in the DBBM group when compared to the HA-Alg-Gel and Control groups in the µCT analysis during all analysis periods. However, no differences were observed in the mineralized tissue/biomaterial proportion observed on day 1 (immediate postoperative) in comparison to later periods of analysis in all groups. In the histomorphometric analysis, the HA-Alg-Gel and Control groups showed higher bone formation than the DBBM group. Moreover, in histological analysis, five samples of the HA-Alg-Gal group exhibited formed bone spicules adjacent to the graft granules against only two of eight samples in the DBBM group. Both graft materials ensured the maintenance of defect bone thickness, while a tissue thickness reduction was observed in the control group. In conclusion, this study demonstrated the osteoconductive potential of HA-Alg-Gel bone graft by supporting new bone formation around its particles.


Resumo Este estudo teve como objetivo avaliar o potencial osteogênico de um compósito de hidroxiapatita (HA), alginato (Alg) e gelatina (Gel) em um modelo de defeito de tamanho crítico em ratos. Vinte e quatro ratos machos foram divididos em três grupos: um controle negativo sem tratamento (grupo controle), um controle positivo tratado com osso bovino desproteinizado (grupo DBBM) e o grupo experimental tratado com o novo compósito HA-Alg-Gel (grupo HA-Alg-Gel). Um defeito de tamanho crítico (8,5mm) foi feito na calvária dos ratos, e a formação óssea foi avaliada por análise de microtomografia computadorizada in vivo (µCT) após 1, 15, 45 e 90 dias. Após 90 dias, os animais foram eutanasiados e análises histológicas e histomorfométricas foram realizadas. Uma maior proporção de tecido mineralizado/biomaterial foi observada no grupo DBBM quando comparado aos grupos HA-Alg-Gel e controle na análise de µCT durante todos os períodos de análise. Entretanto, não foram observadas diferenças na proporção tecido mineralizado/biomaterial no dia 1 (pós-operatório imediato) em relação aos períodos posteriores de análise em todos os grupos. Na análise histomorfométrica, os grupos HA-Alg-Gel e controle apresentaram maior formação óssea do que o grupo DBBM. Além disso, na análise histológica, cinco amostras do grupo HA-Alg-Gal exibiram espículas ósseas formadas adjacentes aos grânulos do enxerto contra apenas duas das oito amostras do grupo DBBM. Ambos os materiais de enxerto garantiram a manutenção da espessura óssea do defeito, enquanto uma redução da espessura do tecido foi observada no grupo controle. Em conclusão, este estudo demonstrou o potencial osteocondutor do enxerto ósseo de HA-Alg-Gel, promovendo a formação de osso novo ao redor das suas partículas.

4.
Acta cir. bras ; Acta cir. bras;39: e392824, 2024. tab, graf, ilus
Article in English | LILACS, VETINDEX | ID: biblio-1563652

ABSTRACT

Purpose: to evaluate biocompatibility and osteogenic potential of hydroxyapatite/alginate composite after its implantation on rat calvarian critical bone defect. Methods: thirty adults male Wistar rats were randomly distributed into two groups: GHA ­ critical bone defect filled with hydroxyapatite/alginate composite granules (HA/Alg) and CG ­ critical bone defect without biomaterial; evaluated at biological points of 15, 45 and 120 days. Results: the histomorphometrically analyses for GHA showed osteoid matrix deposition (OM) among the granules and towards the center of the defect in centripetal direction throughout the study, with evident new bone formation at 120 days, resulting in filling 4/5 of the initial bone defect. For CG, this finding was restricted to the edges of the bone margins and formation of connective tissue on the residual area was found in all biological points. Inflammatory response on GHA was chronic granulomatous type, discrete and regressive for all biological points. Throughout the study, the CG presented mononuclear inflammatory infiltrate diffuse and regressive. Histomorphometry analyses showed that OM percentage was evident for GHA group when compared to CG group in all analyzed periods (p > 0.05). Conclusions: the biomaterial evaluated at this study showed to be biocompatible, bioactive, osteoconductive and biodegradable synchronously with bone formation.


Subject(s)
Animals , Rats , Biocompatible Materials , Bone Regeneration , Durapatite , Alginates
5.
Rev Bras Ortop (Sao Paulo) ; 55(3): 278-283, 2020 Jun.
Article in English | MEDLINE | ID: mdl-32616971

ABSTRACT

Objective To compare the effect of two therapeutic ultrasound protocols, with different times of exposure in the regeneration of critical bone defect. Methods Forty-five male rats were distributed among three experimental groups: therapeutic ultrasound group 5 minutes (TUG 5); therapeutic ultrasound group 10 minutes (TUG 10); and control group (CG). In all groups, a critical bone defect of 8.5 mm diameter was made in the calvaria region. The protocol was initiated on the 1 st postoperative day in TUGs 5 and 10, with therapeutic ultrasound at the frequency of 1.0 MHz, pulsed mode, five times a week, at periods of 15, 30, and 60 days. Results Among the experimental groups, the highest volume of neoformation of osteoid matrix took place in the TUG 10 group followed by TUG 5, when compared with the CG group, in which the neoformation was restricted to the border region. The use of ultrasound promoted an increase in the thickness of the conjunctive matrix, proliferation of capillaries, alignment of the collagen fibers, reduction of edema and inflammatory process, being more significant in the 10-minutes time period. Conclusion Therapeutic ultrasound stimulated the repair of a critical bone defect, and the longer exposure time promoted greater osteogenic stimulation.

6.
Rev. Bras. Ortop. (Online) ; 55(3): 278-283, May-June 2020. graf
Article in English | LILACS | ID: biblio-1138028

ABSTRACT

Abstract Objective To compare the effect of two therapeutic ultrasound protocols, with different times of exposure in the regeneration of critical bone defect. Methods Forty-five male rats were distributed among three experimental groups: therapeutic ultrasound group 5 minutes (TUG 5); therapeutic ultrasound group 10 minutes (TUG 10); and control group (CG). In all groups, a critical bone defect of 8.5 mm diameter was made in the calvaria region. The protocol was initiated on the 1st postoperative day in TUGs 5 and 10, with therapeutic ultrasound at the frequency of 1.0 MHz, pulsed mode, five times a week, at periods of 15, 30, and 60 days. Results Among the experimental groups, the highest volume of neoformation of osteoid matrix took place in the TUG 10 group followed by TUG 5, when compared with the CG group, in which the neoformation was restricted to the border region. The use of ultrasound promoted an increase in the thickness of the conjunctive matrix, proliferation of capillaries, alignment of the collagen fibers, reduction of edema and inflammatory process, being more significant in the 10-minutes time period. Conclusion Therapeutic ultrasound stimulated the repair of a critical bone defect, and the longer exposure time promoted greater osteogenic stimulation.


Resumo Objetivo Comparar o efeito de dois protocolos de ultrassom terapêutico com diferentes tempos de exposição para regeneração de defeito ósseo crítico. Métodos Foram utilizados 45 ratos, machos, distribuídos em três grupos: grupo ultrassom terapêutico 5 minutos (GUS 5); grupo ultrassom terapêutico 10 minutos (GUS 10); e grupo controle (GC). Em todos os grupos, confeccionou-se um defeito ósseo crítico, com 8,5 mm de diâmetro, na região da calvária. O protocolo foi iniciado no 1º dia do pós-operatório, no GUS 5 e no GUS 10, com ultrassom terapêutico na frequência de 1,0 MHz, modo pulsado, 5 vezes por semana, nos períodos de 15, 30, e 60 dias. Resultados Dentre os grupos experimentais, houve maior neoformação de matriz osteoide no GUS 10, seguido do GUS 5 quando comparados ao GC, no qual a neoformação foi restrita à região de borda. O uso do ultrassom promoveu aumento na espessura da matriz conjuntiva, proliferação de capilares, alinhamento das fibras colágenas, redução do edema e do processo inflamatório, tendo sido mais significativo no tempo de 10 minutos. Conclusão O ultrassom terapêutico estimulou o reparo do defeito ósseo crítico, e o maior tempo de exposição promoveu maior estímulo osteogênico.


Subject(s)
Animals , Rats , Skull , Ultrasonic Therapy , Bone and Bones , Bone Regeneration , Morbidity , Collagen
7.
Rev. Ciênc. Méd. Biol. (Impr.) ; 18(2): 239-248, nov 07, 2019. fig, ilus
Article in Portuguese | LILACS | ID: biblio-1291689

ABSTRACT

Introdução: associadas à composição química, as propriedades físicas dos biomateriais como formato, dimensões e porosidade, são fatores que influenciam na biocompatibilidade, biodegradabilidade e nos potenciais osteoindutivo e osteocondutores. Objetivo: identificar modificações físicas no formato, dimensões e porosidade de um biomaterial com nova composição de wollastonita e beta fosfato tricálcico, implantado em defeitos ósseos críticos em calvária de ratos. Metodologia: trata-se de um estudo exploratório, com um biomaterial compósito, antes e após a sua implantação in vivo. Utilizou-se explantes cirúrgicos de calvárias de ratos que receberam implantes em defeito ósseo crítico. Os animais foram distribuídos em 3 grupos experimentais aleatoriamente, compostos por 5 animais cada, e avaliados com 15, 45 e 120 dias. Os grupos foram denominados: G0-biomaterial; GI-15 dias; GII-45 dias; e, GIII-120 dias. As amostras foram micrografadas em microscópio eletrônico de varredura, com diferentes magnificações. Utilizouse o software livre ImageJ para processamento das imagens e análise das partículas e poros. Resultados: a distribuição espacial do biomaterial promoveu preenchimento completo do defeito ósseo crítico. Micrografias com diferentes magnificações permitiu identificar modificações na área de contato entre grânulos, diminuição dos poros intergranular, aumento da área ocupada pelos poros e diminuição das partículas nos grânulos. Nos grupos GII e GIII, identificou-se formação de componentes orgânicos na superfície do implante. Conclusão: houve modificações físicas no formato, dimensões horizontais, distribuição espacial e porosidade da superfície dos grânulos do biomaterial, relacionadas com a biodegradabilidade e o potencial osteoindutivo e osteocondutor do biomaterial.


Introduction: associated with the chemical composition, physical properties of biomaterials related to their format, dimensions and porosity, are factors that influence on biocompatibility, biodegradability and osteoinductive and osteoconductive potential. Objective: identify physical changes in the format, dimensions and porosity of biomaterial with new composition of wollastonite and beta tricalcium phosphate, implanted in critical bone defects in calvaria of rats. Methodology: this is an exploratory study, with a composite biomaterial before and after its implantation in vivo. Used surgical explants of rat calvarium that received biomaterial implants into the critical bone defect. The animals were divided into 3 experimental groups of random distribution, composed of 5 animals each, and evaluated for 15, 45 and 120 days. The groups were named: G0-Biomaterial; GI-15 days; GII-45 days; and, GIII-120 days. The samples were studied by micrography in scanning electronic microscope with different magnifications. It was used the free software ImageJ to image processing and particles and pores analysis. Results: the spatial distribution of the biomaterial promoted full filling of the critical bone defect. Micrographs with different magnifications allowed to identification of modifications in the area of contact between granules, decrease of the intergranular pores, increase of the area occupied by the pores and decrease of the particles in the granules. In GII, and GIII groups, it was identified formation of organic components on the surface of the implant. Conclusion: there were physical modifications in the format, horizontal dimensions, spatial distribution and porosity of the biomaterial, related to the biodegradability and the osteoinductive and osteoconductive potential of the biomaterial.


Subject(s)
Bone Regeneration
8.
Acta Cir Bras ; 34(2): e201900203, 2019 Feb 28.
Article in English | MEDLINE | ID: mdl-30843936

ABSTRACT

PURPOSE: To analyze, histomorphologically, the influence of the geometry of nanostructured hydroxyapatite and alginate (HAn/Alg) composites in the initial phase of the bone repair. METHODS: Fifteen rats were distributed to three groups: MiHA - bone defect filled with HAn/Alg microspheres; GrHA - bone defect filled with HAn/Alg granules; and DV - empty bone defect; evaluated after 15 days postoperatively. The experimental surgical model was the critical bone defect, ≅8.5 mm, in rat calvaria. After euthanasia the specimens were embedded in paraffin and stained with hematoxylin and eosin, picrosirius and Masson-Goldner's trichrome. RESULTS: The histomorphologic analysis showed, in the MiHA, deposition of osteoid matrix within some microspheres and circumjacent to the others, near the bone edges. In GrHA, the deposition of this matrix was scarce inside and adjacent to the granules. In these two groups, chronic granulomatous inflammation was noted, more evident in GrHA. In the DV, it was observed bone neoformation restricted to the bone edges and formation of connective tissue with reduced thickness in relation to the bone edges, throughout the defect. CONCLUSION: The geometry of the biomaterials was determinant in the tissue response, since the microspheres showed more favorable to the bone regeneration in relation to the granules.


Subject(s)
Alginates/chemistry , Biocompatible Materials/chemistry , Bone Regeneration/drug effects , Durapatite/chemistry , Nanostructures/chemistry , Alginates/administration & dosage , Animals , Biocompatible Materials/administration & dosage , Bone Regeneration/physiology , Durapatite/administration & dosage , Male , Nanostructures/administration & dosage , Rats , Rats, Wistar , Spectrophotometry, Infrared
9.
Acta cir. bras. ; 34(2): e201900203, Feb. 28, 2019. ilus, tab, graf
Article in English | VETINDEX | ID: vti-20589

ABSTRACT

Purpose:To analyze, histomorphologically, the influence of the geometry of nanostructured hydroxyapatite and alginate (HAn/Alg) composites in the initial phase of the bone repair.Methods:Fifteen rats were distributed to three groups: MiHA - bone defect filled with HAn/Alg microspheres; GrHA - bone defect filled with HAn/Alg granules; and DV - empty bone defect; evaluated after 15 days postoperatively. The experimental surgical model was the critical bone defect, ≅8.5 mm, in rat calvaria. After euthanasia the specimens were embedded in paraffin and stained with hematoxylin and eosin, picrosirius and Masson-Goldners trichrome.Results:The histomorphologic analysis showed, in the MiHA, deposition of osteoid matrix within some microspheres and circumjacent to the others, near the bone edges. In GrHA, the deposition of this matrix was scarce inside and adjacent to the granules. In these two groups, chronic granulomatous inflammation was noted, more evident in GrHA. In the DV, it was observed bone neoformation restricted to the bone edges and formation of connective tissue with reduced thickness in relation to the bone edges, throughout the defect.Conclusion:The geometry of the biomaterials was determinant in the tissue response, since the microspheres showed more favorable to the bone regeneration in relation to the granules.(AU)


Subject(s)
Animals , Rats , Durapatite/analysis , Nanostructures , Alginates/ultrastructure , Microspheres , Polymers , Bone and Bones/surgery , Bone Regeneration
10.
Acta cir. bras ; Acta cir. bras;34(2): e201900203, 2019. tab, graf
Article in English | LILACS | ID: biblio-989053

ABSTRACT

Abstract Purpose: To analyze, histomorphologically, the influence of the geometry of nanostructured hydroxyapatite and alginate (HAn/Alg) composites in the initial phase of the bone repair. Methods: Fifteen rats were distributed to three groups: MiHA - bone defect filled with HAn/Alg microspheres; GrHA - bone defect filled with HAn/Alg granules; and DV - empty bone defect; evaluated after 15 days postoperatively. The experimental surgical model was the critical bone defect, ≅8.5 mm, in rat calvaria. After euthanasia the specimens were embedded in paraffin and stained with hematoxylin and eosin, picrosirius and Masson-Goldner's trichrome. Results: The histomorphologic analysis showed, in the MiHA, deposition of osteoid matrix within some microspheres and circumjacent to the others, near the bone edges. In GrHA, the deposition of this matrix was scarce inside and adjacent to the granules. In these two groups, chronic granulomatous inflammation was noted, more evident in GrHA. In the DV, it was observed bone neoformation restricted to the bone edges and formation of connective tissue with reduced thickness in relation to the bone edges, throughout the defect. Conclusion: The geometry of the biomaterials was determinant in the tissue response, since the microspheres showed more favorable to the bone regeneration in relation to the granules.


Subject(s)
Animals , Male , Rats , Biocompatible Materials/chemistry , Durapatite/chemistry , Nanostructures/chemistry , Alginates/chemistry , Spectrophotometry, Infrared , Biocompatible Materials/administration & dosage , Bone Regeneration/drug effects , Bone Regeneration/physiology , Rats, Wistar , Durapatite/administration & dosage , Nanostructures/administration & dosage , Alginates/administration & dosage
11.
Rev. Ciênc. Méd. Biol. (Impr.) ; 17(3): 359-368, nov 19, 2018. ilus
Article in Portuguese | LILACS | ID: biblio-1247808

ABSTRACT

Introdução: a regeneração e o reparo de tecidos ósseos perdidos é objeto de estudo da Bioengenharia Tecidual. O uso de biomateriais substitutos ósseos biomiméticos visa estimular os sistemas celulares e bioquímicos para restabelecer de modo mais eficiente o tecido ósseo nos casos de sua reconstrução. Ao investigar o processo de remodelação, é vital identificar áreas de novo crescimento para avaliar a eficácia dos biomateriais implantados e respectivos regimes de tratamento. A avaliação qualitativa e quantitativa da regeneração óssea pode ser realizada através da aplicação de marcadores como o Xilenol, a Tetraciclina, a Calceína e a Alizarina. A administração desses marcadores de forma associada possibilita ainda marcar sequencialmente camadas de nova deposição e remodelação durante o reparo. Objetivo: estabelecer um protocolo para utilização dos marcadores fluorescentes de reparo ósseo xilenol, tetraciclina, calceína e alizarina, em ratos. Metodologia: foram utilizados 35 ratos da linhagem Wistar, machos adultos, com massa corpórea entre 350 e 400g, e idade aproximada de 4 a 5 meses, distribuídos randomicamente em 5 grupos experimentais, submetidos à confecção de defeito ósseo circular de 8 mm em região de calvária, e administração dos diferentes marcadores segundo os grupos; XO ­ Xilenol; Ca ­ Calceína; Al ­ Alizarina; Te ­ Tetraciclina; C ­ Controle. Após 15 dias de experimento, os animais foram eutanasiados e as calvárias processadas e analisadas por histomorfometria, microscopia de epifluorescência e microscopia de fluorescência. Resultados: todos protocolos empregados para utilização dos marcadores fluorescentes xilenol, calceína, alizarina e tetracicilina foram úteis para identificar área de deposição mineral durante o período analisado de regeneração óssea em ratos. As imagens obtidas pela microscopia de fluorescência revela a presença dos marcadores incorporados à matriz óssea neoformada, no entanto a utilização da Alizarina e Calceína dentro dos protocolos testados mostraram-se mais eficientes. Conclusão: os protocolos testados nesse estudo apresentaram-se viáveis para utilização em pesquisas envolvendo marcadores de regeneração óssea, com resultados superiores para Alizarina e Calceína


Introduction: The regeneration and repair of lost bone tissues is the subject of a study of Tissue Bioengineering. The use of biomimetic biomaterial bone substitutes aims to stimulate the cellular and biochemical systems to restore more efficiently the bone tissue in the cases of its reconstruction. When investigating the remodeling process, it is vital to identify areas of new growth to evaluate the efficacy of implanted biomaterials and their treatment regimens. The qualitative and quantitative evaluation of bone regeneration can be performed through the use of markers such as Xylenol, Tetracycline, Calcein and Alizarin. The administration of such markers in an associated manner also makes it possible to sequentially mark layers of new deposition and remodeling during the repair. Objective: to establish a protocol for the use of fluorescent xylenol, tetracycline, calcein and alizarin bone repair markers in rats. Metodology: thirtyfive male adult Wistar rats with a body mass ranging from 350 to 400 g and approximately 4 to 5 months old were randomly assigned to 5 experimental groups submitted to a circular bone defect of 8 mm in the region of calvaria, and administration of the different markers according to the groups; XO ­ Xylenol; Ca ­ Calcein; Al-Alizarin; Te ­ Tetracycline; C ­ Control. After 15 days of experiment, the animals were euthanized and the calvaria processed and analyzed by histomorphometry, epifluorescence microscopy and fluorescence microscopy. Results: all protocols used for fluorescence markers xylenol, calcein, alizarin and tetracycline were useful to identify area of mineral deposition during the analyzed period of bone regeneration in rats. The images obtained by fluorescence microscopy revealed the presence of the markers incorporated into the neoformed bone matrix, however the use of Alizarin and Calcein within the protocols tested were more efficient. Conclusion: the protocols tested in this study were feasible for use in research involving markers of bone regeneration, with superior results for Alizarin and Calcein.


Subject(s)
Animals , Male , Rats , Bone Regeneration/drug effects , Tissue Engineering/methods , Fluorescent Dyes/pharmacology , Tetracycline/pharmacology , Xylenes/pharmacology , Random Allocation , Pilot Projects , Rats, Wistar , Disease Models, Animal , Microscopy, Fluorescence
12.
Rev. Ciênc. Méd. Biol. (Impr.) ; 17(2): 164-169, maio -jun. 2018. tab, ilus
Article in Portuguese | LILACS | ID: biblio-1224366

ABSTRACT

Introdução: a face é o centro estético do indivíduo. Por isso, lesões nessa região podem repercutir mais negativamente do que em qualquer outra parte do corpo. Os cianoacrilatos, devido às suas vantagens de uso, incluindo a ausência de marcas de ponto, apresentam-se como um atrativo método de fechamento de feridas faciais em suas mais diversas formas. Objetivo: avaliação da eficácia do fechamento de excisões cutâneas faciais, usando o 2-etilcianoacrilato. Metodologia: estudo retrospectivo, no qual 36 feridas foram ocluídas com 2-etilcianoacrilatocomo alternativa à sutura intradérmica. Resultados: feridas excisionais (100%) foram tratadas com 2-etilcianoacrilato e suturas profundas relaxadoras como método de síntese. Cicatrizes inestéticas (13,9%), deiscência (5,6%) e infecção (2,8%) foram os problemas encontrados. Não houve casos de necrose, reações alérgicas ou de queloide. Os resultados foram considerados satisfatórios na maioria dos pacientes (96,4%). Conclusão: o uso do 2-etilcianoacrilato se mostrou seguro e com excelentes resultados cosméticos no grupo de pacientes selecionados.


Introduction: the face is the aesthetic center of the individual. Therefore, lesions in this region may have more negative repercussions than in any other part of the body. Cyanoacrylates (CA), due to their advantages of use, including the absence of dot marks, present themselves as an attractive method of closing facial wounds in their most diverse forms. Objective: to evaluate the efficacy of the closure of facial skin excisions using 2-ethylcyanoacrylate. Methodology: retrospective study in which 36 wounds were occluded with 2-ethylcyanoacrylate as an alternative to intradermal suture. Results: excisional wounds (100%) were treated with 2-ethylcyanoacrylate and deep relaxation sutures as a synthesis method. Unaesthetic scars (13.9%), dehiscence (5.6%) infection (2.8%) were the problems encountered. There were no cases of necrosis, allergic or keloid reactions. The results were considered satisfactory in most patients (96.4%). Conclusion: the use of 2-ethylcyanoacrylate was safe and with excellent cosmetic results in this group of patients.


Subject(s)
Face
13.
Rev. Ciênc. Méd. Biol. (Impr.) ; 16(3): 393-395, dez 19, 2017. tab, fig
Article in Portuguese | LILACS | ID: biblio-1342010

ABSTRACT

Introdução: o estudo do piramidal, a despeito do tamanho reduzido e da função rudimentar desse músculo, tem revelado interesse renovado devido à sua utilidade em diversas condições clínicas. Objetivo: identificação do músculo piramidal em um grupo de cadáveres de mestiços provenientes das duas principais regiões metropolitanas do estado da Bahia (Salvador e Feira de Santana). Metodologia: estudo retrospectivo de necropsias nas quais os piramidais foram expostos para visualização e contagem direta. Resultados: músculo piramidal bilateral: 73,3%; unilateral: 5%; ausente: 21,7%. Conclusões: os achados sugerem que a presença do músculo foi correlacionada fracamente com características demográficas regionais em uma população miscigenada.


Introduction: the study of the pyramidal, despite the small size and rudimentary muscle function, has revealed renewed interest due to your usefulness in various clinical conditions. Objective: pyramidal muscle identification in a group of corpses of mestizos of various ethnicities from the two main metropolitan regions of the state of Bahia (Salvador and Feira de Santana). Methodology: retrospective study of necropsies in which pyramidal muscles were exposed to view and direct count. Results: bilateral pyramidal muscle: 73.3%, unilateral: 5% and absent: 21.7%. Conclusions: the findings suggest that the presence of muscle was weakly correlated by demographic characteristics in a racially mixed population regional.


Subject(s)
Abdominal Muscles
14.
Rev. Ciênc. Méd. Biol. (Impr.) ; 16(3): 277-282, dez 19, 2017. tab, fig
Article in Portuguese | LILACS | ID: biblio-1293064

ABSTRACT

Introdução: estudos que relacionam qualidade de vida com abdominoplastia demonstram aspectos positivos na vida desses pacientes. O abdômen é uma das cirurgias mais solicitadas após grande perda ponderal. E as cirurgias devem ser indicadas individualizadas. Objetivo: avaliar qual perfil dos pacientes submetidos à abdominoplastia pós-bariátrica no C-HUPES no período de 2008-2015 e comparar dados dos prontuários do C-HUPES com dados disponibilizados pelo DATASUS. Metodologia: trata-se de um estudo observacional, transversal realizado em um hospital universitário no estado da Bahia. Após seleção dos pacientes os dados foram coletados dos prontuários e instrumentos de avaliação padronizados foram aplicados durante avaliação no ambulatório. Foi realizada ainda coleta de dados e estudo de série temporal com dados agregados e secundários oriundos do sistema DATASUS e do Sistema de Informações Hospitalares/SIH. Foram analisadas todas as cirurgias bariátricas e pós-bariátricas realizadas no estado da Bahia no mesmo período de coleta do C-HUPES no período de 2008 a 2015. Resultado: o peso no período da dermolipectomia variou entre 55,4 e 111,8 kg (média de 86,361 Kg) apresentando cerca de 50 kg a menos que antes da gastroplastia. Na avaliação pós-operatória no ambulatório foi constatado uma média de idade de 49 anos, A altura da cicatriz com relação ao púbis foi de 9,1 cm e foi observada uma pequena assimetria entre a distância da extremidade da cicatriz e o eixo central variando de 0 a 6 cm. A média do comprimento da cicatriz horizontal foi de 57,8 cm. Conclusão: identificado uma demanda muito grande de pacientes que necessitam de tratamento pós-gastroplastia. As pacientes são operadas com idade diferente do esperado pela idade das gastroplastias, houve pouco ganho de peso no pós-operatório tardio mesmo operando pacientes com IMC elevado. Foi identificado o uso de códigos de dermolipectomias não estéticas quando poderia ser usado o de cirurgia pós-bariátrica onerando o custo com internamento hospital.


Introduction: studies that relate quality of life with abdominoplasty demonstrate positive results in patients' quality of life. The abdomen is one of the most requested surgeries after major weight loss. Propouse: the aim of this study was to evaluate the profile of patients who underwent post-bariatric abdominoplasty from C-HUPES in the period 2008-2015 and to compare these data from C-HUPES with data provided by DATASUS. Methodology: the cross-sectional, observational study performed at a university hospital in the state of Bahia. After the selection of the patients the data were collected from the medical records and the standardized evaluation instruments were applied during the outpatient evaluation. We also performed data collection and time series study with aggregated and secondary data from the DATASUS system and the Hospital Information System / SIH. All bariatric and post-bariatric surgeries performed in the state of Bahia without period of collection at C-HUPES Hospital from 2008 to 2015 were analyzed. Results: weight without period of dermolipectomy ranged from 55.4 to 111.8 kg ( Average of 86.361 kg) presenting about 50 kg less than before gastroplasty. In the postoperative evaluation without ambulatory, an average age of 49 years was observed. The height of the scar with respect to the pubis was of 9.1 cm and a small asymmetry was observed between the distance between the ends of the scar and the central axis of the scar. 0 to 6 cm. The mean horizontal scar length was 57.8 cm. Conclusion: a very large need was identified for patients requiring treatment after gastroplasties. As patients are operated at age and different from expected by the age of gastroplasty, there was some weight gain without late postoperative even when operating with patients with high imc. It was identified as the use of non-esthetic dermolipectomy codes where the postoperative surgery could be used in the hospital


Subject(s)
Surgery, Plastic , Abdominoplasty
15.
Rev. paul. odontol ; 32(2): 39-44, abr.-jun. 2010.
Article in Portuguese | LILACS, BBO - Dentistry | ID: lil-576250

ABSTRACT

A expectativa de vida da população brasileira é maior hoje do que no passado, o que implica em uma demanda crescente de cuidados periodontais. O presente artigo de atualização tem por objetivo divulgar as alterações do periodonto e da perda óssea, frequentes durante o processo de envelhecimento correlacionando-os à osteoporose principalmente em mulheres pós-menopausadas...


The life expectancy of brazilian population is currently greater than in the past, which implies in a growing demand for periodontal care. This update article aims to evaluate changes of periodontal supporting tissues and lost of bone tissue during the process of aging, correlating them with osteoporosis especially in postmenopausal women...


Subject(s)
Alveolar Bone Loss , Bone Diseases , Osteoporosis
16.
Periodontia ; 16(1): 89-94, mar. 2006.
Article in Portuguese | LILACS, BBO - Dentistry | ID: lil-518576

ABSTRACT

Originalmente, a Matriz Dermal (MOA) Acelular foi introduzida na medicina, em cirurgia plástica, para o tratamento de feridas de queimaduras de espessura total (terceiro grau). Na periodontia, esta matriz é utilizada como uma alternativa para o enxerto de mucosa palatal autógena, com o intuito de se obter um aumento na largura da faixa de gengiva inserida; para o tratamento de recessões gengivais e recobrimento de raízes; como membrana protetora para regeneração óssea guiada dentre outras. Esta matriz dermal alógena é acelularizada, congelada e ressecada, constituída por um complexo de membrana basal e matriz extracelular estruturalmente integradas, na qual fibras colágenas e fibras elásticas são os principais componentes. A MOA apresenta muitas vantagens, dentre elas, a redução da morbidade e maior conforto para o paciente, por eliminar um segundo sítio cirúrgico, estética desejável. quantidade disponível ilimitada, menor dor pós-operatória e maior aceitação da terapia cirúrgica pelo paciente pediátrico. Baseado nesses aspectos, o propósito deste trabalho é compilar dados descritos em trabalhos científicos sobre a Matriz Dermal Acelular (Alloderm@) e suas aplicações na Bioengenharia Tecidual Periodontal. com o objetivo de esclarecer as modalidades de sua aplicação em cirurgias plásticas periodontais, que podem utilizar essa matriz como fonte doadora de tecido.


Subject(s)
Surgery, Oral
17.
Cells Tissues Organs ; 184(3-4): 128-37, 2006.
Article in English | MEDLINE | ID: mdl-17409738

ABSTRACT

The aim of this study was to evaluate the osteogenic behavior of two chemically similar bioactive glass products (Biogranand Perioglas) implanted in critical bone defects in rat calvaria. Thirty-six transfixed bone defects of 8 mm diameter were made surgically in adult male Wistar rats. The animals were distributed equally into three groups: Biogran (GI), Perioglas (GII) and without implant material (control; GIII). The morphology and composition of both bioactive glasses were analyzed by scanning electron microscopy and energy-dispersive spectrometry. Tissue specimens were analyzed at the biological time points of 15, 30 and 60 days by optical microscopy and morphometry, demonstrating biocompatibility for the tested materials with moderate chronic inflammation involving their particles. Bone neoformation resulted only as a reparative reaction to an intentionally produced defect and was limited to the defect's edges. No statistically significant differences among the groups were observed. At the scar interstice, abundant deposits of collagenous fibers enveloping the particles were noted. The present results indicated that the bioactive glasses, under the experimental conditions analyzed, did not show osteogenic behavior.


Subject(s)
Glass , Prostheses and Implants , Skull/anatomy & histology , Animals , Bone Cements , Bone Substitutes , Male , Rats , Rats, Wistar , Skull/cytology , Spectrophotometry
18.
Periodontia ; 15(3): 16-21, jul.-set. 2005.
Article in Portuguese | LILACS, BBO - Dentistry | ID: lil-500799

ABSTRACT

Recentes avanços na área de engenharia tecidual possibilitaram o desenvolvimento e aperfeiçoamento de novos biomateriais de implantes aloplásticos de diferentes composições químicas.Dentre os biomateriais cerâmicos de fosfato de cálcio, os biovidros destacam-se em função do seu comportamento biológico e adesão aos tecidos ósseos. O presente trabalho tem como objetivo revisar as propriedades fisico-químicas e mecanismo de ação deste biomaterial, enfatizando sua atuação na regeneração óssea.


Subject(s)
Biocompatible Materials , Bone Regeneration
19.
Rev. Ciênc. Méd. Biol. (Impr.) ; 4(1): 70-76, jan.-abr. 2005.
Article in Portuguese | LILACS, BBO - Dentistry | ID: lil-472236

ABSTRACT

A possibilidade de regeneração de tecidos e órgãos com algum tipo de dano biológico tem-se constituído numa preocupação notável ao longo dos séculos. Reconstruções ósseas adequadas dependem da regeneração tecidual desencadeada pela atividade de células-tronco mesenquimais. No entanto, o conhecimento da origem e das características fenotípicas dessas células, como também dos fatores que as governam, durante as etapas de formação e remodelação ósseas, é limitado. Técnicas de investigação sobre a sua replicação ex vivo e posterior implantação in vivo estão, atualmente, sendo implementadas. Por sua vez, recentes avanços na área de biomateriais têm concentrado esforços para a sua apropriada aplicação clínica. Este trabalho tem como objetivo revisar os aspectos mais relevantes acerca do emprego das células-troncomesenquimais nas estratégias da bioengenharia tecidual óssea.


Subject(s)
Mesenchymal Stem Cells , Biomedical Engineering , Bone and Bones , Bone Regeneration , Bone Remodeling
20.
Rev. Ciênc. Méd. Biol. (Impr.) ; 2(2): 276-282, jul.-dez. 2003. ilus
Article in Portuguese | LILACS, BBO - Dentistry | ID: biblio-855810

ABSTRACT

Os autores relatam caso de recessão gengival na dentição decídua, uma ocorrência incomum, e discutem aspectos clínicos, fatores determinantes e predisponentes ao aparecimento do problema mucogengival, e o plano de tratamento com medidas interceptativas e preventivas


Subject(s)
Humans , Female , Child, Preschool , Pacifiers/adverse effects , Deglutition , Tooth, Deciduous , Gingival Recession , Open Bite
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