ABSTRACT
Resonance energy transfer studies using a pyrene-labeled phospholipid derivative 1-palmitoyl-2-[10-(pyren-1-yl)decanoyl]-sn-glycero-3-phosphoglycerol (donor) and the heme (acceptor) of cytochrome c (cyt c) have indicated that ATP causes changes in the conformation of the lipid-bound protein (Rytömaa, M., Mustonen, P., and Kinnunen, P. K. J. (1992) J. Biol. Chem. 267, 22243-22248). Accordingly, after binding cyt c via its so called C-site to neat phosphatidylglycerol liposomes (mole fraction of PG = 1.0) has commenced, further quenching of donor fluorescence is caused by ATP, saturating at 2 mm nucleotide. ATP-induced conformational changes in liposome-associated cyt c could be directly demonstrated by CD in the Soret band region (380-460 nm). The latter data were further supported by time-resolved spectroscopy using the fluorescent cyt c analog with a Zn(2+)-substituted heme moiety. A high affinity ATP-binding site has been demonstrated in cyt c (Craig, D. B., and Wallace, C. J. A. (1993) Protein Sci. 2, 966-976) that is compromised by replacing the invariant Arg(91) to norleucine. Although no major effects on conformation and function of cyt c were concluded due to the modification, a significantly reduced effect by ATP on the lipid-bound [Nle(91)]cyt c was evident, implying that this modulation is mediated via the Arg(91)-containing binding site.
Subject(s)
Adenosine Triphosphate/metabolism , Cytochrome c Group/metabolism , Lipid Metabolism , Animals , Apoptosis , Arginine/chemistry , Binding Sites , Chromatography, High Pressure Liquid , Circular Dichroism , Dose-Response Relationship, Drug , Horses , Hydrogen-Ion Concentration , Liposomes/metabolism , Models, Molecular , Norleucine/chemistry , Protein Binding , Protein Conformation , Spectrometry, Fluorescence , Zinc/chemistryABSTRACT
c-Raf-1 is a major effector of Ras proteins, responsible for activation of the ERK MAP kinase pathway and a critical regulator of both normal growth and oncogenic transformation. Using an inducible form of Raf in MDCK cells, we have shown that sustained activation of Raf alone is able to induce the transition from an epithelial to a mesenchymal phenotype. Raf promoted invasive growth in collagen gels, a characteristic of malignant cells; this was dependent on the operation of an autocrine loop involving TGFbeta, whose secretion was induced by Raf. TGFbeta induced growth inhibition and apoptosis in normal MDCK cells: Activation of Raf led to inhibition of the ability of TGFbeta to induce apoptosis but not growth retardation. ERK has been reported previously to inhibit TGFbeta signaling via phosphorylation of the linker region of Smads, which prevents their translocation to the nucleus. However, we found no evidence in this system that ERK can significantly influence the function of Smad2, Smad3, and Smad4 at the level of nuclear translocation, DNA binding, or transcriptional activation. Instead, strong activation of Raf caused a broad protection of these cells from various apoptotic stimuli, allowing them to respond to TGFbeta with increased invasiveness while avoiding cell death. The Raf-MAP kinase pathway thus synergizes with TGFbeta in promoting malignancy but does not directly impair TGFbeta-induced Smad signaling.
Subject(s)
Epithelial Cells/pathology , Proto-Oncogene Proteins c-raf/metabolism , Transforming Growth Factor beta/metabolism , Animals , Apoptosis/drug effects , Apoptosis/physiology , Cell Division , Cell Line , Collagen , DNA-Binding Proteins/metabolism , Dogs , Epithelial Cells/drug effects , Epithelial Cells/metabolism , Mesoderm , Mitogen-Activated Protein Kinase 1/metabolism , Mitogen-Activated Protein Kinase Kinases/metabolism , Phosphorylation , Proto-Oncogene Proteins c-raf/genetics , Recombinant Proteins/drug effects , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Signal Transduction , Smad2 Protein , Smad3 Protein , Tamoxifen/analogs & derivatives , Tamoxifen/pharmacology , Trans-Activators/metabolismABSTRACT
Detachment of epithelial cells from extracellular matrix results in induction of apoptosis ('anoikis') which can be blocked by expression of activated Ras or PKB/Akt. Here we show that detachment causes release of cytochrome c from mitochondria in MDCK cells. This is blocked by caspase inhibitors, suggesting a role for caspases upstream of mitochondria in the initiation of anoikis, in accord with the ability of dominant negative FADD to inhibit this form of cell death. Bulk activation of caspase-8 following detachment lags behind cytochrome c release, and is likely the result of a mitochondrial positive feed back loop. Matrix detachment also induces Bax translocation to mitochondria in a caspase-dependent manner. Expression of activated Ras or PKB/Akt blocks all the detectable events on the detachment-induced apoptosis signalling pathway, suggesting that PKB/Akt acts at an early point in the pathway, providing the signal normally generated by matrix attachment. Strong activation of Raf can also protect MDCK cells from detachment induced apoptosis, but this occurs at a point downstream of cytochrome c release from mitochondria, and is clearly distinct from the effect of PKB/Akt. Oncogene (2000) 19, 4461 - 4468.
Subject(s)
Arabidopsis Proteins , Cytochrome c Group/metabolism , Extracellular Matrix/metabolism , Mitochondria/metabolism , Protein Serine-Threonine Kinases , Proto-Oncogene Proteins c-bcl-2 , Proto-Oncogene Proteins c-raf/metabolism , Proto-Oncogene Proteins/metabolism , Animals , Apoptosis/physiology , Caspase 8 , Caspase 9 , Caspase Inhibitors , Caspases/metabolism , Cell Line , Cysteine Proteinase Inhibitors/pharmacology , Dogs , Epithelial Cells/cytology , Epithelial Cells/drug effects , Epithelial Cells/metabolism , Fatty Acid Desaturases/genetics , Fatty Acid Desaturases/metabolism , Mitochondria/drug effects , Proto-Oncogene Proteins c-akt , Proto-Oncogene Proteins c-raf/genetics , Signal Transduction , Tamoxifen/analogs & derivatives , Tamoxifen/pharmacology , bcl-2-Associated X Protein , ras Proteins/metabolismABSTRACT
Detachment of most untransformed adherent cells from the extracellular matrix promotes apoptosis, in a process termed anoikis [1] [2]. The death signalling mechanisms involved in this process are not known, although adhesion or transformation by ras oncogenes have been shown to protect epithelial cells from apoptosis through activation of phosphatidylinositol 3-kinase and protein kinase B (PKB/Akt) [3]. Here we show that detachment-induced apoptosis (anoikis) is blocked by the expression of a dominant-negative form of FAS-associated death domain protein (FADD) in a number of untransformed epithelial cell lines. Because the soluble extracellular domains of the death receptors CD95, DR4 and DR5 failed to block anoikis, we conclude that ligand-dependent activation of these death receptors is not involved in this process. Detachment induced strong activation of caspase 8 and caspase 3. Detachment-induced caspase-8 activation did not require the function of downstream caspases but was blocked by overexpression of the anti-apoptotic proteins Bcl-2 or Bcl-X(L). We propose that caspase-8 activation is the initiating event in anoikis, which is subsequently subject to a positive-feedback loop involving mitochondrial events.
Subject(s)
Apoptosis/physiology , Arabidopsis Proteins , Caspases/physiology , Cell Adhesion/physiology , Fatty Acid Desaturases/physiology , Signal Transduction/physiology , Amino Acid Chloromethyl Ketones/pharmacology , Animals , Caspase 3 , Caspase 8 , Caspase 9 , Caspase Inhibitors , Cell Line , Coumarins/metabolism , Cysteine Proteinase Inhibitors/pharmacology , Dogs , Enzyme Activation/drug effects , Epithelial Cells/cytology , Extracellular Matrix/physiology , Kidney , Oligopeptides/metabolism , Oligopeptides/pharmacology , Protein Structure, Tertiary , Proto-Oncogene Proteins c-bcl-2/biosynthesis , Proto-Oncogene Proteins c-bcl-2/genetics , Proto-Oncogene Proteins c-bcl-2/physiology , Receptors, TNF-Related Apoptosis-Inducing Ligand , Receptors, Tumor Necrosis Factor/physiology , Signal Transduction/drug effects , bcl-X Protein , fas Receptor/physiologyABSTRACT
A large number of pharmaceutically active compounds have a high affinity to acidic phospholipids; good examples are the cationic compounds lidocaine, propranolol, and gentamycin. These drugs influenced the lipid dynamics of liposomes composed of phosphatidylcholine and the acidic phosphatidylglycerol, as judged by the excimer/monomer emission intensity ratio for a pyrene-labeled phospholipid analog, as well as by polarization of DPH fluorescence. When the mole fraction X of PG (XPG) was 0.20, lidocaine increased membrane fluidity. The opposite was true for propranolol, which caused the formation of pyrene lipid-enriched microdomains. Gentamycin had no apparent effect. At XPG = 1.00, all these drugs rigidified membrane. Subsequently, we investigated the detachment of a cationic peripheral membrane protein, cytochrome c (cyt c), by these compounds from liposomes. This was accomplished by monitoring resonance energy transfer from a pyrene-labeled phospholipid to the heme of cyt c. The efficiency of the above compounds to dissociate cyt c varied considerably. In brief, significantly lower concentrations of gentamycin than propranolol or lidocaine were required for half-maximal dissociation of cyt c from liposomes, although the final extent of protein detachment by gentamycin was less complete. ATP augmented the dissociation of cyt c from membranes by lidocaine and propranolol. Stopped-flow measurements also revealed that the half-times differed for the release of cyt c from the membranes. Our results are likely to reflect differences in the contributions of the electrostatic interactions and hydrophobicity to the drug/lipid interaction and comply with two different acidic phospholipid binding sites in cyt c.
Subject(s)
Cytochrome c Group/metabolism , Gentamicins/metabolism , Gentamicins/pharmacology , Lidocaine/metabolism , Lidocaine/pharmacology , Membrane Proteins/metabolism , Phospholipids/metabolism , Propranolol/metabolism , Propranolol/pharmacology , Binding, Competitive , Cations , Cytochrome c Group/drug effects , Gentamicins/chemistry , Hydrogen-Ion Concentration , Kinetics , Lidocaine/chemistry , Liposomes , Phospholipids/chemistry , Propranolol/chemistryABSTRACT
In the single membrane of Acholeplasma laidlawii a specific glucosyltransferase synthesize the major, lamellar-forming lipid diglucosyldiacylglycerol (DGlcDAG) from the major, nonlamellar-prone monoglucosyldiacylglycerol (MGlcDAG). This is crucial for the maintenance of phase equilibria close to a bilayer-nonbilayer transition and a nearly constant spontaneous curvature in the membrane lipid bilayer. Acyl chain order is also affected, but not kept constant. Phosphatidylglycerol (PG) is an essential activator, needed in substantial amounts by the DGlcDAG synthase, and likely to affect bilayer properties. A potential connection was investigated between the (i) lateral diffusion, (ii) domain formation of the PG activator and (iii) bilayer chain ordering (i.e., the hydrocarbon free volume), revealed in unilamellar liposomes by lipid probes containing one or two (fluorescent) pyrene acyl chains, and (iv) activity of the DGlcDAG synthase. Different activator, nonbilayer perturbant, and bilayer matrix conditions were employed. Diffusion of PG was substantially slower in a DGlcDAG compared to a phosphatidylcholine (PC) matrix with 18:1c chains but increased with the PG content in both. No obvious correlation between diffusion and enzyme activity, and no local concentration of PG as a function of chain ordering or curvature, was detected. However, an enrichment of PG activator into domains could be induced by a chain length mismatch between 18:1c-PG and 14:1c-PC (but not 22:1c-PC), even at small PG fractions. Patching was sufficient to stimulate enzyme activity 4-fold in relation to the activities normally valid at low PG concentrations. Chain order was substantially lower (i.e., free volumes larger) in bilayers of DGlcDAG than in bilayers of PC and increased in an additive fashion in both by the content of especially the nonbilayer-prone 1,3-18:1c-DAG but also by PG. At physiological concentrations of PG in DGlcDAG bilayers (approximately 20%) a good correlation was evident between increased DAG content and chain ordering and strongly enhanced enzyme activities, with maxima close to a bilayer-nonbilayer transition. It is concluded that regulation of packing conditions in A. laidlawii membranes by the DGlcDAG synthase seems to be governed not by the absolute extent of chain order but more by the spontaneous curvature within a certain range of conditions. Domain formation of the essential PG activator due to bilayer conditions is a second mechanism, potentially overriding the curvature effects.
Subject(s)
Acholeplasma laidlawii/enzymology , Glucosyltransferases/metabolism , Glycolipids/biosynthesis , Lipid Bilayers/metabolism , Membrane Lipids/metabolism , Acholeplasma laidlawii/growth & development , Cell Membrane/enzymology , Cell Membrane/metabolism , Indicators and Reagents , Kinetics , Lipid Bilayers/chemical synthesis , Spectrometry, Fluorescence , Substrate SpecificityABSTRACT
Tacrine (1,2,3,4-tetrahydro-9-acridinamine monohydrate) is an inhibitor of acetylcholinesterase currently used in the treatment of the symptoms of Alzheimer's disease. The present study demonstrates preferential binding of this drug to acidic phospholipids, as revealed by fluorescence polarization, penetration into lipid monolayers, and effects on the thermal phase behavior of dimyristoyl phosphatidic acid (DMPA). A fivefold enhancement in the polarization of tacrine emission is evident above the main phase transition temperature (T(m)) of DMPA vesicles, whereas below T(m) only a 0.75-fold increase is observed. In contrast, the binding of tacrine to another acidic phospholipid, dimyristoylphosphatidylglycerol, did not exhibit strong dependence on T(m). In accordance with the electrostatic nature of the membrane association of tacrine, the extent of binding was augmented with increasing contents of egg PG in phosphatidylcholine liposomes. Furthermore, [NaCl] > 50 mM dissociates tacrine (albeit incompletely) from the liposomes composed of acidic phospholipids. Inclusion of the cationic amphiphile sphingosine in egg PG vesicles decreased the membrane association of tacrine until at 1:1 sphingosine: egg PG stoichiometry binding was no longer evident. Tacrine also penetrated into egg PG but not into egg PC monolayers. Together with broadening of the main transition and causing a shoulder on its high temperature side, the binding of tacrine to DMPA liposomes results in a concentration-dependent reduction both in the combined enthalpy delta H of the above overlapping endotherms and the main transition temperature T(m). Interestingly, these changes in the thermal phase behavior of DMPA as a function of the content of the drug in vesicles were strongly nonlinear. More specifically, upon increasing [tacrine], T(m) exhibited stepwise decrements. Simultaneously, sharp minima in delta H were observed at drug:lipid stoichiometries of approximately 2:100 and 25:100, whereas a sharp maximum in delta H was evident at 18:100. The above results are in keeping with tacrine causing phase separation processes in the bilayer and may also relate to microscopic drug-induced ordering processes within the membrane.
Subject(s)
Phospholipids/chemistry , Tacrine/chemistry , Cholinesterase Inhibitors/chemistry , Dimyristoylphosphatidylcholine , Kinetics , Phosphatidylcholines , Phosphatidylethanolamines , Phosphatidylglycerols , Phosphatidylinositols , Sphingosine , Structure-Activity RelationshipABSTRACT
The basic chromosomal protein histone H1 binds avidly to liposomes containing acidic phospholipids and with characteristics somewhat resembling the lipid association of cytochrome c (cyt c) [Koiv et al. (1995) Biochemistry 34, 8018-8027]. Membrane association of histone H1 strongly attenuates the lipid lateral diffusion in large unilamellar vesicles containing phosphatidylglycerol (PG) as revealed by the decrease in the excimer to monomer ratio Ie/Im of the pyrene fatty acid-containing phospholipid derivative 1-palmitoyl-2-[10-(pyren-1-yl)decanoyl]-sn-glycero-3-phosphogly cer ol (PPDPG) fluorescence. Similarly, an increase in fluorescence anisotropy of the membrane-incorporated probe, diphenylhexatriene (DPH), due to histone H1 indicates that the membrane becomes more rigid. Increasing the mole fraction of PG (XPG) increases in a linear manner the concentration [H1]s required for the maximal decrease in Ie/Im or increase in fluorescence anisotropy, thus allowing to estimate the binding site for H1 to be constituted by approximately 20 PG molecules. Domain formation is also supported by differential scanning calorimetry measurements. Subsequently, we studied the detachment of cyt c from PG-containing liposomes by H1 by measuring its efficiency in decreasing resonance energy transfer between PPDPG and the heme of cyt c. The A-site interaction of 1 microM cyt c with 25 microM PG/PC (XPG = 0.20) liposomes is fully inhibited by low (0.1 microM) histone concentrations. Upon XPG being increased, the concentration [H1]D required for complete detachment of cyt c increases. Irrespective of the [cyt c] present (varying between 0.1 and 10 microM), the C-site-mediated binding of cyt c to neat PG liposomes (XPG = 1.0) is fully prevented at [H1] = 0.6 microM. These measurements indicate that the affinity of histone H1 to liposomes exceeds that of cyt c. The above effects of H1 were subsequently compared with those of different basic membrane-associating peptides. Notably, the effects of HI were remarkably well-reproduced by polylysine (K19). The high affinity of H1 to acidic phospholipids suggests that this feature might also contribute to its physiological function.
Subject(s)
Cytochrome c Group/metabolism , Histones/metabolism , Liposomes , Peptides/metabolism , Amino Acid Sequence , Animals , Binding Sites , Cattle , Cosyntropin/chemistry , Cosyntropin/metabolism , Cytochrome c Group/chemistry , Energy Transfer , Fluorescence Polarization , Fluorescent Dyes/chemistry , Histones/chemistry , In Vitro Techniques , Liposomes/chemistry , Molecular Sequence Data , Peptide Fragments/chemistry , Peptide Fragments/metabolism , Peptides/chemistry , Phosphatidylglycerols/chemistry , Polylysine/chemistry , Polylysine/metabolism , Protein Binding , alpha-MSH/analogs & derivatives , alpha-MSH/chemistry , alpha-MSH/metabolismABSTRACT
Studies on the membrane binding of cytochrome c revealed liposome-associated and soluble cytochrome c not to be in rapid equilibrium. In brief, cytochrome c attached to pyrene phospholipid-labeled, fluorescent liposomes containing either 17.6 mol % cardiolipin (CL) or 30 mol % egg phosphatidylglycerol (PG) is practically not at all or very slowly, respectively, detached by a subsequently added excess (up to 20-fold) of nonlabeled liposomes containing these acidic lipids. Cytochrome c was fully dissociated from PG-containing liposomes by increasing the ionic strength by NaCl, whereas dissociation from CL-containing membranes was less complete, presumably because of the scavenging of the protein within inverted intramembrane micelles. Importantly, the apparent irreversibility of the binding of cytochrome c to liposomes is strongly dependent on the structure of the acidic phospholipid. Cytochrome c bound to lyso-PG/PC liposomes could be dissociated with an excess of nonlabeled PG-containing liposomes. Cytochrome c was also efficiently bound to membranes containing the negatively charged dicetylphosphate yet could be readily dissociated by nonlabeled PG-containing liposomes. We conclude both proper geometry of the phosphate group and the presence of two acyl chains to be required for the tight binding of cytochrome c to acidic phospholipids. These data provide evidence for the membrane association of cytochrome c by an acidic phospholipid in the extended conformation (Kinnunen, P. K. J., Köiv, A., Lehtonen, J. Y. A., Rytömaa, M., and Mustonen, P. (1994) Chem. Phys. Lipids 73, 181-207) in which one of the acyl chains of the lipid becomes accommodated within a hydrophobic cavity of the protein. Based on the crystal structure of cytochrome c we putatively assign the invariant Asn-52 (horse heart cytochrome c) as the site liganding the protonated phosphate of the lipid, whereas Lys-72 and -73 should bind the deprotonated form.
Subject(s)
Cytochrome c Group/chemistry , Cytochrome c Group/metabolism , Liposomes , Asparagine , Cardiolipins , Energy Transfer , Hydrogen Bonding , Kinetics , Lysophospholipids , Micelles , Models, Structural , Molecular Conformation , Phosphatidylcholines , Phosphatidylglycerols , Protein Binding , Protein Conformation , Spectrometry, Fluorescence , Structure-Activity RelationshipABSTRACT
A large body of evidence strongly indicates biomembranes to be organized into compositionally and functionally specialized domains, supramolecular assemblies, existing on different time and length scales. For these domains and intimate coupling between their chemical composition, physical state, organization, and functions has been postulated. One important constituent of biomembranes are peripheral proteins whose activity can be controlled by non-covalent binding to lipids. Importantly, the physical chemistry of the lipid interface allows for a rapid and reversible control of peripheral interactions. In this review examples are provided on how membrane lipid (i) composition (i.e., specific lipid structures), (ii) organization, and (iii) physical state can each regulate peripheral binding of proteins to the lipid surface. In addition, a novel and efficient mechanism for the control of the lipid surface association of peripheral proteins by [Ca2+], lipid composition, and phase state is proposed. The phase state is, in turn, also dependent on factors such as temperature, lateral packing, presence of ions, metabolites and drugs. Confining reactions to interfaces allows for facile and cooperative large scale integration and control of metabolic pathways due to mechanisms which are not possible in bulk systems.
Subject(s)
Membrane Lipids/chemistry , Membrane Lipids/physiology , Membrane Proteins/chemistry , Membrane Proteins/physiology , Models, Biological , Animals , Annexins/chemistry , Choline-Phosphate Cytidylyltransferase , Cytochrome c Group/chemistry , Liposomes , Membrane Potentials , Membranes/chemistry , Membranes/physiology , Nucleotidyltransferases/chemistry , Peptides/chemistry , Phospholipids/chemistry , Phospholipids/physiology , Surface PropertiesABSTRACT
Binding of cytochrome c (cyt c) to cardiolipin/phosphatidylcholine (CL/PC) and phosphatidylglycerol/PC (PG/PC) liposomes was studied at neutral pH utilizing fluorescence resonance energy transfer from a membrane-incorporated pyrene phospholipid derivative to the heme of cyt c. ATP in millimolar concentrations displaced nearly quantitatively cyt c from membranes containing 17.5 mol% CL or 30 mol% PG. Notably, increasing the acidic phospholipid/PC molar ratio in the liposomes progressively reduced the membrane detachment of cyt c by ATP, and practically no dissociation of cyt c from neat PG or CL liposomes was observed. Complete dissociation of cyt c from PG/PC liposomes was also produced by subsequently added NaCl. However, the concentration of salt required for half-maximal effect increased upon increasing the PG/PC molar ratio. At 0.1 M NaCl no binding of cyt c to neat PG liposomes was observed whereas the extent of membrane association of cyt c increased with increasing CL/PC molar ratios also in the presence of salt. This difference between CL and PG is attributed to the complex electrostatics of the former lipid resulting in its high affinity for protons. The above results can be rationalized in terms of two acidic phospholipid-binding sites in cyt c. The electrostatically interacting site is constituted by basic residues in cyt c and could be identical to the ATP-binding site (Craig, D. B., and Wallace, C. J. A. (1993) Protein Sci. 2, 966-976). In addition there should be another lipid-binding site in cyt c with a high affinity to protonated acidic phospholipids. Both sites appear to be available for lipid binding at neutral bulk pH.
Subject(s)
Cytochrome c Group/chemistry , Phospholipids/metabolism , Adenosine Triphosphate/metabolism , Binding Sites , Cardiolipins/metabolism , Cytochrome c Group/metabolism , Hydrogen-Ion Concentration , Kinetics , Lipid Bilayers , Liposomes , Osmolar Concentration , Phosphatidylcholines/metabolism , Phosphatidylglycerols/metabolism , Spectrometry, Fluorescence , Structure-Activity RelationshipABSTRACT
Resonance energy transfer was measured between egg phosphatidylcholine liposomes containing the intramolecular excimer forming pyrene-labelled phospholipid analogue 1,2-bis[pyren-1-(-yl)]decanoyl-sn-glycero-3-phosphocholine (bisPDPC) as a donor and DNA-bound adriamycin as an acceptor. Membrane association of DNA turned out to be critically dependent on the presence of sphingosine in the liposomes. Identical result was obtained by measuring the extent of quenching of the fluorescent DNA-bound dye Hoechst 33258 due to energy transfer to the lipophilic stain Nile Red incorporated in egg phosphatidylcholine liposomes containing varying amounts of sphingosine. The attachment of DNA to sphingosine-containing membranes could be reversed by the further inclusion of the negatively charged phosphatidic acid up to approximately 1:2 PA/sphingosine molar ratio in the liposomes, thus suggesting the involvement of electrostatic interactions. Differential scanning calorimetry measurements confirmed a lack of association between DNA and dimyristoylphosphatidylcholine liposomes. Instead drastic changes were produced by DNA in the heat capacity scans measured for liposomes also incorporating sphingosine. Fluorescence microscopy revealed an extensive aggregation of sphingosine containing pyrene-phosphatidylcholine-labelled egg phosphatidylcholine liposomes in the presence of DNA. Together with other available data on the effects of sphingosine, the present findings suggest that sphingosine could directly alter the chromatin structure. Accordingly, such alterations may contribute to the control of replication and gene expression.
Subject(s)
DNA/chemistry , Liposomes , Membrane Fusion , Phosphatidic Acids , Phosphatidylcholines , Sphingosine , Bisbenzimidazole , Calorimetry, Differential Scanning , Doxorubicin , Microscopy, Fluorescence , Spectrometry, FluorescenceABSTRACT
Membrane association of cytochrome c (cyt c) was monitored by the efficiency of resonance energy transfer from a pyrene-fatty acid containing phospholipid derivative (1-palmitoyl-2[6-(pyren-1-yl)]hexanoyl-sn-glycero-3-phosphocholine (PPHPC)) to the heme of cyt c. Liposomes consisted of 85 mol% egg phosphatidylcholine (egg PC), 10 mol% cardiolipin, and 5 mol% PPHPC. Cardiolipin was necessary for the membrane binding of cyt c over the pH range studied, from 4 to 7. In accordance with the electrostatic nature of the membrane association of cyt c at neutral pH both 2 mM MgCl2 and 80 mM NaCl dissociated cyt c from the vesicles completely. At neutral pH also adenine nucleotides in millimolar concentrations were able to displace cyt c from liposomes, their efficiency decreasing in the sequence ATP > ADP > AMP. In addition, both CTP and GTP were equally effective as ATP. The detachment of cyt c from liposomes by nucleotides is likely to result from a competition between cardiolipin and the nucleotides for a common binding site in cyt c. When pH was decreased to 4 there was a small yet significant increase in the apparent affinity of cyt c to cardiolipin containing liposomes. Notably, at pH 4 the above nucleotides as well as NaCl and MgCl2 were no longer able to dissociate cyt c and, on the contrary, they slightly enhanced the quenching of pyrene fluorescence by cyt c. The above results do suggest that the membrane association of cyt c at acidic pH was non-ionic and presumably due to hydrogen bonding. The pH-dependent binding of cyt c to membranes was fully reversible. Accordingly, in the presence of sufficient concentrations of either nucleotides or salts rapid detachment and membrane association of cyt c could be induced by varying pH between neutral and acidic values, respectively.