ABSTRACT
This study investigates the potential of humic substances (HS) and graphene oxide (GO), as extracellular electron acceptors (EEA) for nitrification, aiming to explore alternatives to sustain this process in wastewater treatment systems. Experimental results demonstrate the conversion of ammonium to nitrate (up to 87 % of conversion) coupled to the reduction of either HS or GO by anaerobic consortia. Electron balance confirmed the contribution of HS and GO to ammonium oxidation. Tracer analysis in incubations performed with 15NH4+ demonstrated 15NO3- as the main product with a minor fraction ending as 29N2. Phylogenetic analysis identified Firmicutes, Euryarchaeota, and Chloroflexi as the microbial lineages potentially involved in anoxic nitrification linked to HS reduction. This study introduces a new avenue for research in which carbon-based materials with electron-accepting capacity may support the anoxic oxidation of ammonium, for instance in bioelectrochemical systems in which carbon-based anodes could support this novel process.
Subject(s)
Carbon , Nitrification , Carbon/chemistry , Electrons , Graphite/chemistry , Phylogeny , Oxidation-Reduction , Ammonium Compounds/metabolism , Anaerobiosis , Nitrates/metabolism , Bacteria/metabolismABSTRACT
Inland waters are crucial in the carbon cycle, contributing significantly to the global CO2 fluxes. Carbonate lakes may act as both sources and sinks of CO2 depending on the interactions between the amount of dissolved inorganic carbon (DIC) inputs, lake metabolisms, and geochemical processes. It is often difficult to distinguish the dominant mechanisms driving CO2 dynamics and their effects on CO2 emissions. This study was undertaken in three groundwater-fed carbonate-rich lakes in central Spain (Ruidera Lakes), severely polluted with nitrates from agricultural overfertilization. Diel and seasonal (summer and winter) changes in CO2 concentration (CCO2) DIC, and CO2 emissions-(FCO2)-, as well as physical and chemical variables, including primary production and phytoplanktonic chlorophyll-a were measured. In addition, δ13C-DIC, δ13C-CO2 in lake waters, and δ13C of the sedimentary organic matter were measured seasonally to identify the primary CO2 sources and processes. While the lakes were consistently CCO2 supersaturated and FCO2 was released to the atmosphere during both seasons, the highest CCO2 and DIC were in summer (0.36-2.26 µmol L-1). Our results support a strong phosphorus limitation for primary production in these lakes, which impinges on CO2 dynamics. External DIC inputs to the lake waters primarily drive the CCO2 and, therefore, the FCO2. The δ13C-DIC signatures below -12 confirmed the primary geogenic influence on DIC. As also suggested by the high values on the calcite saturation index, the Miller-Tans plot revealed that the CO2 source in the lakes was close to the signature provided by the fractionation of δ13C-CO2 from calcite precipitation. Therefore, the main contribution behind the CCO2 values found in these karst lakes should be attributed to the calcite precipitation process, which is temperature-dependent according to the seasonal change observed in δ13C-DIC values. Finally, co-precipitation of phosphate with calcite could partly explain the observed low phytoplankton production in these lakes and the impact on the contribution to increasing greenhouse gas emissions. However, as eutrophication increases and the soluble reactive phosphorus (SRP) content increases, the co-precipitation of phosphate is expected to be progressively inhibited. These thresholds must be assessed to understand how the CO32- ions drive lake co-precipitation dynamics. Carbonate regions extend over 15% of the Earth's surface but seem essential in the CO2 dynamics at a global scale.
Subject(s)
Carbon Dioxide , Lakes , Seasons , Lakes/chemistry , Carbon Dioxide/analysis , Carbon Dioxide/metabolism , Spain , Environmental Monitoring/methods , Carbon Cycle , Phytoplankton/metabolismABSTRACT
Availability of fixed nitrogen is a pivotal driver on primary productivity in the oceans, thus the identification of key processes triggering nitrogen losses from these ecosystems is of major importance as they affect ecosystems function and consequently global biogeochemical cycles. Denitrification and anaerobic ammonium oxidation coupled to nitrite reduction (Anammox) are the only identified marine sinks for fixed nitrogen. The present study provides evidence indicating that anaerobic ammonium oxidation coupled to the reduction of sulfate, the most abundant electron acceptor present in the oceans, prevails in marine sediments. Tracer analysis with 15N-ammonium revealed that this microbial process, here introduced as Sulfammox, accounts for up to 5 µg 15N2 produced g-1 day-1 in sediments collected from the eastern tropical North Pacific coast. Raman and X-ray diffraction spectroscopies revealed that elemental sulfur and sphalerite (ZnFeS) were produced, besides free sulfide, during the course of Sulfammox. Anaerobic ammonium oxidation linked to Fe(III) reduction (Feammox) was also observed in the same marine sediments accounting for up to 2 µg 15N2 produced g-1 day-1. Taxonomic characterization, based on 16S rRNA gene sequencing, of marine sediments performing the Sulfammox and Feammox processes revealed the microbial members potentially involved. These novel nitrogen sinks may significantly fuel nitrogen loss in marine environments. These findings suggest that the interconnections among the oceanic biogeochemical cycles of N, S and Fe are much more complex than previously considered.
Subject(s)
Ammonium Compounds/metabolism , Ferric Compounds/metabolism , Geologic Sediments/chemistry , Nitrogen/analysis , Seawater/microbiology , Sulfates/metabolism , Anaerobiosis , Bacteria/metabolism , Biodegradation, Environmental , Electrons , Iron/analysis , Oxidation-Reduction , Sulfur/metabolismABSTRACT
Ecological systems may occur in alternative states that differ in ecological structures, functions and processes. Resilience is the measure of disturbance an ecological system can absorb before changing states. However, how the intrinsic structures and processes of systems that characterize their states affects their resilience remains unclear. We analyzed time series of phytoplankton communities at three sites in a floodplain in central Spain to assess the dominant frequencies or "temporal scales" in community dynamics and compared the patterns between a wet and a dry alternative state. The identified frequencies and cross-scale structures are expected to arise from positive feedbacks that are thought to reinforce processes in alternative states of ecological systems and regulate emergent phenomena such as resilience. Our analyses show a higher species richness and diversity but lower evenness in the dry state. Time series modeling revealed a decrease in the importance of short-term variability in the communities, suggesting that community dynamics slowed down in the dry relative to the wet state. The number of temporal scales at which community dynamics manifested, and the explanatory power of time series models, was lower in the dry state. The higher diversity, reduced number of temporal scales and the lower explanatory power of time series models suggest that species dynamics tended to be more stochastic in the dry state. From a resilience perspective our results highlight a paradox: increasing species richness may not necessarily enhance resilience. The loss of cross-scale structure (i.e. the lower number of temporal scales) in community dynamics across sites suggests that resilience erodes during drought. Phytoplankton communities in the dry state are therefore likely less resilient than in the wet state. Our case study demonstrates the potential of time series modeling to assess attributes that mediate resilience. The approach is useful for assessing resilience of alternative states across ecological and other complex systems.
Subject(s)
Biodiversity , Ecosystem , Phytoplankton , Floods , Geography , Models, Theoretical , Seasons , SpainABSTRACT
This study evaluates whether the size structure of seston (the sum of living and nonliving particles in the water column) reflects the effects of fish on wetland water quality. Using enclosures, we measured water quality and zooplankton community structure in the presence and absence of two fish species with distinct foraging strategies [benthivorous carp (Cyprinus carpio) and planktivorous mosquitofish (Gambusia holbrooki)]. Seston collected from the enclosures was counted and sized automatically with a Coulter counter, and the size structure in the range of 1-60 microm was modelled using the underlying Pareto distribution of particles. Only Cyprinus contributed to a loss of water quality (increased nutrient levels, algal and non-algal turbidity, hypoxia), while both fish species changed zooplankton community composition compared to fishless controls. However, these changes were not reflected in parameters [goodness of fit (r (2)) and parameter c (slopes)] of Pareto models. Multivariate statistics suggest that seston size structure responded more to environmental gradients related to water depth but the relationships were also weak. Our overall result contrasts with the regulation of size spectra constructed from living organisms. Although seston integrates many structural and functional features of the water column, the lack of strong relationships between Pareto model parameters and water quality suggests that a size-based approach to characterise seston structure has a limited potential for assessing biota-mediated effects in wetlands in a straightforward manner.
Subject(s)
Carps/metabolism , Cyprinodontiformes/metabolism , Water Pollutants, Chemical/analysis , Wetlands , Animals , Evaluation Studies as Topic , Models, Theoretical , Particle Size , Species Specificity , Zooplankton/metabolismABSTRACT
Water requirements to supply human needs lead water stakeholders to store more water during surplus periods to fulfil the demand during--not only--scarcity periods. At the reservoirs, mostly those in semi-arid regions, water level then fluctuates extremely between rises and downward during one single year. Besides of water management implications, changes on physical, chemical and biological dynamics of these drawdown and refilling are little known yet. This paper shows the results, throughout a year, on solids, nutrients (N and P), chlorophyll-a, and sedimentation changes on the dynamics, when the former policy was applied in a reservoir from the semi-arid Northwestern Mexico. Water level sinusoidal trend impinged changes on thermal stratification and mixing, modifying nutrient cycling and primary producer responses. According to nitrogen and phosphorus concentration as well as chlorophyll-a, reservoir was mesotrophic, becoming hypertrophic during drawdown. Nutrient concentrations were high (1.22 +/- 0.70 and 0.14 +/- 0.12 mg P l(-1)), increasing phosphorus and lowering N:P significantly throughout the study period, although no intensive agricultural, no urban development, neither industrial activities take place in the watershed. This suggests nutrient recycling complex mechanisms, including nutrient release from the sediment-water interface as the main nutrient pathway when shallowness, at the same time as mineralization, increases. Outflows controlled nitrogen and phosphorus availability on the ecosystem while organic matter depended on river inflows. As on other subtropical aquatic ecosystems, nitrogen limited primary productivity (Spearman correlation R = 0.75) but chlorophyll-a seasonal pattern showed an irregular trend, prompting other no-nutrient related limitants. Shallowness induced a homogeneous temporal pattern on water quality. This observed temporal variability was mainly explained statistically by changes on solids (mineral and organic), chlorophyll-a and flows (62.3%). Annual sedimentation rates of total solids ranged from 11.73 to 16.29 kg m(-2) year(-1) with organic matter comprising around 30%. N:P ratio on sedimentation rates were as high as could be expected in a resuspension dominated ecosystem, and spatially inverse related with N:P ratio on bottom sediments. Distance from river inlet into the reservoir reveals a marked spatial heterogeneity on solid and nitrogen sedimentation, showing the system dependence on river inflows and supporting resuspension as the main phosphorus pathway. Accretion rates (2.19 +/- 0.40 cm year(-1)) were not related to hydrological variability but decreased with the distance to the river input. Total sediment accumulation (9,895 tons km(-2) year(-1)) denotes siltation as other serious environmental problem in reservoirs but possibly not related with operational procedures.