Characterization of terminal flowering cowpea (Vigna unguiculata (L.) Walp.) mutants obtained by induced mutagenesis digs out the loss-of-function of phosphatidylethanolamine-binding protein.

Cowpea (Vigna unguiculata (L.) Walp) is one of the major food legume crops grown extensively in arid and semi-arid regions of the world. The determinate habit of cowpea has many advantages over the indeterminate and is well adapted to modern farming systems. Mutation breeding is an active research area to develop the determinate habit of cowpea. The present study aimed to develop new determinate habit mutants with terminal flowering (TFL) in locally well-adapted genetic backgrounds. Consequently, the seeds of popular cowpea cv P152 were irradiated with doses of gamma rays (200, 250, and, 300 Gy), and the M1 populations were grown. The M2 populations were produced from the M1 progenies and selected determinate mutants (TFLCM-1 and TFLCM-2) from the M2 generation (200 Gy) were forwarded up to the M5 generation to characterize the mutants and simultaneously they were crossed with P152 to develop a MutMap population. In the M5 generation, determinate mutants (80-81 days) were characterized by evaluating the TFL growth habit, longer peduncles (30.75-31.45 cm), erect pods (160°- 200°), number of pods per cluster (4-5 nos.), and early maturity. Further, sequencing analysis of the VuTFL1 gene in the determinate mutants and MutMap population revealed a single nucleotide transversion (A-T at 1196 bp) in the fourth exon and asparagine (N) to tyrosine (Y) amino acid change at the 143rd position of phosphatidylethanolamine-binding protein (PEBP). Notably, the loss of function PEPB with a higher confidence level modification of anti-parallel beta-sheets and destabilization of the protein secondary structure was observed in the mutant lines. Quantitative real-time PCR (qRT-PCR) analysis showed that the VuTFL1 gene was downregulated at the flowering stage in TFL mutants. Collectively, the insights garnered from this study affirm the effectiveness of induced mutation in modifying the plant's ideotype. The TFL mutants developed during this investigation have the potential to serve as a valuable resource for fostering determinate traits in future cowpea breeding programs and pave the way for mechanical harvesting.

Implementation of Pediatric Allergic Rhinitis Module as a Part of AETCOM among First-Year Medical Undergraduates: Mixed Methods Evaluation.

Background: Children suffering from allergic rhinitis (AR) in their earlier days of life, not receiving proper treatment, subsequently develop asthma. To sensitize the first-year medical undergraduates about AR by implementing pediatric allergic rhinitis (PAR) module as a part of their attitude, ethics, and communication (AETCOM) curriculum. Materials and Methods: Triangulation type of mixed method study was conducted from January 2021 to June 2021 among 125 first-year medical undergraduate students. The PAR module communication checklist was developed and validated by an interprofessional (IP) team. Twenty multiple-choice questions (MCQs) were framed for both pretest and posttest cognitive assessment of the students. The pretest assessment was done (first 15 min) followed by the teaching of the PAR module (30 min), and lastly the posttest assessment along with open-ended feedback (last 15 min). Objective Structured Clinical Examination (OSCE) communication checklist along with the guidelines was given to the observer during the student-patient encounter to score the learner and to assess the communication skill. Apart from descriptive analysis, paired t-test and content analysis were done. Results: A statistically significant difference in the mean scores before and after the PAR module and communication checklist (P < 0.001). Majority (78/81, 96%) of the students favored this module, while (28/81) 34.6% suggested modifications. Most of the parent's feedback was good about the student's communication skill in terms of empathy (118), behavior (107), and greet (125); however, 33 parents were about the opinion of difficulties in closing the session, 17 parents commented about student's language problem and 27 about feedback. Conclusion: The PAR module should be taught in the current medical curriculum as a part of AETCOM in the foundation course as early clinical exposure with some modifications in the existing module.

Role of Confined Optical Phonons in Exciton Generation in Spherical Quantum Dot.

Optical control of excitonic states in semiconducting quantum dots has enabled it to be deployed as a qubit for quantum information processing. For self-assembled quantum dots, these excitonic states couple with phonons in the barrier material, for which the previous studies have shown that such exciton-phonon coupling can also lead to the generation of exciton, paving the way for their deployment in qubit-state preparation. Previous studies on self-assembled quantum dots comprising polar materials have considered exciton-phonon coupling by treating phonon modes as bulk acoustic modes only, owing to nearly the same acoustic property of the dot and barrier material. However, the dimensional confinement leads to significant modification phonon modes, even though acoustic confinement is weak but optical confinement cannot be overlooked. In this paper, we investigate for the first time the exciton-optical phonon coupling using dielectric continuum model duly accounting for the dimensional confinement leading to exciton generation. We report that at low temperatures (below 10 K), the exciton creation rate attributed to confined optical phonon is approximately 5.7 times (~6) slower than bulk acoustic phonons, which cannot be ignored, and it should be accounted for in determining the effective phonon assisted exciton creation rate.

Thornber-Feynman carrier-optical-phonon scattering rates in wurtzite crystals.

It is well known that the carrier-optical-phonon scattering rates dominate the carrier-acoustic-phonon scattering rates in many polar materials of interest in electronic and optoelectronic applications. Furthermore, it is known that the Fröhlich coupling constants for carrier-optical-phonon in many materials is close to or great than unity, calling into question the validity of scattering rates based on the Fermi golden rule. In a celebrated paper by Thornber and Feynman it was shown that that the large Fröhlich coupling constant in polar materials does indeed lead to substantial corrections to the Fermi golden rule scattering rates. These large corrections are due to the fact that for strong coupling constants, the first-order perturbative approach underlying the Fermi golden rule does not take into account the presence of many phonons interacting simultaneous with the carrier. In this paper, the Thornber-Feymnan scattering rates for carrier-optical-phonon interactions are derived for several technologically important wurtzite semiconductors-BN, ZnO, CdS, CdSe, ZnS, InN, and SiC- and it is shown that the commonly used Fermi golden rule scattering rates must be corrected by factors ranging up to an order-of-magnitude. The corrections to the Fermi golden rule reported herein have widespread impact on carrier transport for materials with large Fröhlich coupling constants.

Anharmonic decay of high-frequency LA modes in quasi-isotropic III-nitrides.

We report for the first time an estimation of the spontaneous decay rates at room temperature in a selection of nitride-based nanostructures that are quasi-isotropic. We numerically calculate the phonon distribution functions and the decay rates and find that the decay channel LA → TA + TA dominates over the decay channel LA → LA + TA, which confirms Klemens' prediction [1] that LA phonon will primarily split into two doubly degenerate TA phonons through a greater variety of decay channels compared to the decay of LA into two modes, one belonging to the longitudinal acoustic and the other to the transverse acoustic branch.

Crouzon Syndrome in a Ten-week-old Infant: A Case Report.

Crouzon syndrome is a rare genetic disorder. We report a rare case of Crouzon syndrome in a very young infant with distinct features of craniofacial malformations. A 10-week-old male child presented with features of craniofacial dysostosis with abnormal shape of the skull, proptosis, hypertelorism, curved nose and frontal bossing. Radiological findings revealed a predominant premature fusion of sagittal sutures. The infant had airway obstruction. Features of hydrocephalus, papilledema and optic atrophy were not evident. We chose to manage the symptoms and prevent complications, and the surgery was deferred for later, depending on the degree of malformation and psychological needs. Diagnosis of this rare condition at an early stage can help in preventing the development of complications. A careful follow-up and appropriate surgical intervention can improve the prognosis of this condition, which carries the risk of respiratory complications, poor vision and, in some cases, mental retardation as the age advances.

Auditory and Visual P300 Responses in Early Cognitive Assessment of Children and Adolescents with Epilepsy.

BACKGROUND: The event-related potential P300 has been suggested to be valuable in the assessment of cognitive dysfunctions. Not a great deal of neurophysiological assessment has been performed at early stages in patients with epilepsy involving visual and auditory P300 measures. AIMS AND OBJECTIVES: This study aimed to assess the cognitive status in patients with epilepsy earlier by visual and auditory P300 and to find their correlation with various risk factors. MATERIALS AND METHODS: P300 was recorded in 60 children with epilepsy in the age-group of 5-18 years and 60 age- and sex-matched controls by a rare-frequent (oddball) paradigm. Mean auditory and visual P300 latencies and amplitudes were compared among patients with epilepsy and controls and among patients with generalized and focal epilepsy by unpaired t-test. Pearson's correlation coefficient test was computed for studying the correlation between risk factors and P300 responses. A value of P < 0.05 was considered statistically significant. RESULTS: Statistically significant delay in P300 latencies and reduction in amplitudes (both visual and auditory) was found in patients with epilepsy as compared to controls and also among patients with generalized and focal epilepsy. In generalized epilepsy, both visual and auditory P300 revealed significant delay, whereas only auditory P300 delay was found in focal form. No significant correlation was obtained with risk factors. No significant difference was found in P300 responses among patients with and without antiepileptic treatment. CONCLUSION: Visual and auditory P300 latencies have an important role in the evaluation of early cognitive dysfunctions in children with epilepsy. P300 potentials are not influenced by antiepileptic treatment, whereas the type of epilepsy alters them.

Effect of music of specific frequency upon the sleep architecture and electroencephalographic pattern of individuals with delayed sleep latency: A daytime nap study.

INTRODUCTION: Sleep is normal human behaviour. However, the stress in daily life leads to altered sleep behaviour like insomnia, parasomnia, etc. Owing to possible side effects, mind-body interventions like music, yoga and meditation could be a better alternative intervention to pharmacological interventions for the condition. It is known that 432 Hz music to have some effect on the overall sleep quality though some knowledge gap does exist. The present study aims to find the effects of 432 Hz on sleep quality and sleep latency in a daytime nap among subjects with history of delayed sleep latency. MATERIAL AND METHOD: Fifteen healthy male volunteers aged 18 to 40 years with history of delayed sleep latency were recruited for the study from a cohort of working staff and students at the institute, after due ethical clearance following the inclusion and exclusion criteria. All the subjects were subjected to sleep study with and without music intervention at the gap of 1 week. Sleep parameters recorded include sleep stages, electroencephalogram (EEG), electrocardiogram (ECG), electromyography (EMG), nasal airflow, thoracic movement, nasal saturation etc. RESULT: Outcome of the study shows some decrease in the mean sleep latency (P > 0.05) with significant increase in the energy of alpha waves (P < 0.01) at the sleep onset. CONCLUSION: It was concluded that 432 Hz music has some significant calming effect as reflected by increased alpha activities without any significant effect upon the sleep latency in the daytime naps.

The effect of short-term exposure to red and blue light on the autonomic tone of the individuals with newly diagnosed essential hypertension.

The research study aimed to study the effect of short term exposure to light basically red, blue and white on the autonomic tone of essential hypertensive individuals. The objective was to find out the baseline cardiac autonomic function along with the effect of these lights on the cardiac autonomic function among them. Till date few if any study have been conducted upon the individuals with certain disorder as common as essential hypertension. This was a cross sectional observational study conducted in the institute itself that included 77 newly diagnosed hypertensive subjects who willingly participated in the study. After written informed consent, brief history taking with the help of self-made questionnaire and clinical examination, they were randomized to different intervention groups (IG) namely IG I (red) IG II (blue) and IG III (white). HRV analysis of the last 5-6 minutes of both the baseline and color exposure was finally analyzed using MS Excel version 13 and Graph Pad Prism version 7.05. Different HRV parameters have been found to be affected differently on different color exposures. Red has shown to have an impact, mainly on the sympathetic system whereas white showed a dominant vagal component thus acting as a parasympathetic regulator. On one hand, where no conclusive result was found on blue light exposure, white light showed the most prominent results affecting various time and frequency components of HRV like SDRR, TP, LF etc. The present study, both, contradicts as well as supports various other works done on the similar area of interest. One reason of such high variation in different results is because HRV is itself a very dynamic function affected by even a slight change in both the internal and external environment of the subject. As artificial lights of various colors are part and parcel of the aesthetics and designing of most of the work environment all over world, it is very pertinent to study its impact upon human health status. The outcome of the study may play a decisive role in the diagnostics and therapeutics of essential hypertension in days to come. Furthermore, on the basis of the present findings, a future study could be undertaken with bigger data base addressing the limitations of the present study to find some conclusive evidence in the area highlighted.

Role of language proficiency and personality traits upon the academic performance of undergraduate medical students.

BACKGROUND: Academic performance of medical students is governed by various factors. Personality traits such as core self-evaluation (CSE) and locus of control (LOC) are a few of the most important factors that could govern academic performance. The medium of communication is another important factor, especially in a country with language diversity that could affect the academic performance of the new medical entrants. The study aims to examine the effect of LOC, CSE score, and English Language proficiency on the academic performance of undergraduate medical students. MATERIALS AND METHODS: Ninety undergraduate medical students of a premier central government medical institute of India has been recruited for the study after due ethical clearance from the Institutional Ethical Committee. A standard questionnaire for all the parameters to be studied had been filled by the participants. The academic performance has been judged on the basis of the professional examination's marks score. RESULTS: The multiple regression analysis considering all the variables, the English language proficiency appears to be the most important factor with R2 = 0.106 and P = 0.003. Most of the academic performers have shown the dominance of internal LOC and moderately high CSE scores though not statistically significant. CONCLUSIONS: Language proficiency appears to be one of the most important determinants of academic performance in the medical entrants of Indian subcontinent. LOC and CSE could be an important yardstick for academic performance, but that needs to be confirmed with another study with a bigger data set.

Effectiveness of early clinical exposure in learning respiratory physiology among the newly entrant MBBS students.

INTRODUCTION: Early Clinical Exposure has been conceptualized to orient medical students towards actual clinical scenario and help them correlate their theoretical knowledge with real life situations in early years of MBBS courses. In the present study we explored the outcome of early clinical exposure in the context of basic science topics (Physiology) in fresh MBBS entrants and compared their performance with a conventionally taught control group. METHODS: One hundred fifty voluntary students of 1st year MBBS (2015-16) batch consisted the sample of this study. They were divided into two groups through the simple random method (using computer generated random number table with roll numbers of the students). They were evaluated by MCQ (Multiple Choice Question) and OSCE (Objective Structured Clinical Examination) before and after being taught a basic Physiology topic (respiratory system) theoretically. The study group underwent clinical exposure before the post-test while the control group did not. Performance of the students was compared between the two groups by unpaired student's t-test whereas marks of pre and post-test within the same group were compared by paired Student's t-test. Everywhere p<0.05 was considered to be significant. RESULTS: The marks of each group in the pre and post-tests differed significantly (P<0.05 in each case). Post-test marks were significantly greater in each group though the level of improvement was strikingly higher in the study group (p=0.01). Though there was no significant difference in pre-test marks of both groups (P=0.73), post-test marks were significantly higher in the study group (P=0.04). Among the exposed students, majority (92%) opined that ECE was a better technique being practically oriented and more interesting while some (8%) found it to be more time and energy-consuming, suitable for selective portions of basic science topics. CONCLUSION: Early clinical exposure may be an effective technique to supplement the traditional theoretical teaching and improve the performance of fresh medical entrants in Physiology. It has better acceptability by the students and may be considered for inclusion in the existing pre-clinical curriculum with proper allocation of time and manpower.

A Comprehensive Review on Methodologies Employed for Visual Evoked Potentials.

Visual information is fundamental to how we appreciate our environment and interact with others. The visual evoked potential (VEP) is among those evoked potentials that are the bioelectric signals generated in the striate and extrastriate cortex when the retina is stimulated with light which can be recorded from the scalp electrodes. In the current paper, we provide an overview of the various modalities, techniques, and methodologies which have been employed for visual evoked potentials over the years. In the first part of the paper, we cast a cursory glance on the historical aspect of evoked potentials. Then the growing clinical significance and advantages of VEPs in clinical disorders have been briefly described, followed by the discussion on the earlier and currently available methods for VEPs based on the studies in the past and recent times. Next, we mention the standards and protocols laid down by the authorized agencies. We then summarize the recently developed techniques for VEP. In the concluding section, we lay down prospective research directives related to fundamental and applied aspects of VEPs as well as offering perspectives for further research to stimulate inquiry into the role of visual evoked potentials in visual processing impairment related disorders.

Cardiac hemodynamic profile and its correlates by Impedance plethysmography in normal individuals of Central India.

Evaluation of hemodynamic profile by impedance plethysmography (IPG) provides functional expression of cardiovascular performance with efficacy of treatment. Objective of this study was to establish normative hemodynamic parameters by IPG. In this cross-sectional study hemodynamic parameters including cardiac output (CO), Stroke volume (SV), left ventricular ejection time (LVET), impedance (Zo) Cardiac index (CI) and Stroke index (SI) of 50 normal individuals in mean age 38.9±15.2 years were evaluated. Mean CO and CI were 4.9±0.8L/min and 3.3±0.7 L/min/m2 respectively. A negative linear co-relation of CI with increasing age and body fat mass was observed. Mean LVET was 336±33ms with a significant positive linear correlation (r = 0.46) with advancing age and body fat mass while a negative linear correlation with BSA. To conclude preliminary normative data of cardiac parameters using IPG, in subjects of central India of different age groups has been presented.

Magnitude of peripheral neuropathy in cirrhosis of liver patients from central rural India.

CONTEXT: Cirrhosis of liver is an important cause of morbidity and mortality and if associated with peripheral neuropathy (PN) it also poses a huge financial, psychological burden for the patients and their families. AIM: The aim of the present study was to study the magnitude of PN among subjects with cirrhosis of liver presenting to tertiary care teaching hospital in central rural India. SETTINGS AND DESIGN: A cross-sectional study was performed in a tertiary care teaching hospital. MATERIALS AND METHODS: In all patients of cirrhosis of liver irrespective of etiology, aged 15 and above, undergone clinical assessment for peripheral nervous systems damage and confirmed by nerve conduction studies. STATISTICAL ANALYSIS USED: We used chi square test to study associations. P value ≤0.05 was considered as significant. Crude odds ratios were computed to assess the strength of association between independent variables and dependent variables along with their 95% confidence intervals. RESULTS: We included 207 of cirrhosis of liver patients admitted in medicine department from November 2010 through November 2013. Nearly 83% patients were male and 63.2% patients were under the age of 45 years. Common features in these patients were ascites (71%) splenomegaly (63.3%) pedal edema (61.4%) icterus (46.4%) tingling (44.9%) gastrointestinal bleeding(39.1%), ataxia (26.6%), numbness(26.6%), distal motor weakness (21.7%) and paresthesia(20.8%). Among the manifestation of peripheral nerve involvement, loss of ankle reflex was the most common feature in 51.7%, followed by loss of temperature sense 29.5%, loss of vibration sense 20.8%, loss of touch 16.4%, loss of position sense 14.5% and loss of pain in 6.3% of the patients. Peripheral neuropathy was found in 53.6% [95% CI: 46.58- 60.56] study subjects on electrophysiological study. CONCLUSIONS: Analysis of electrophysiological study shows that the PN is very common in study subjects with cirrhosis of liver, especially in male subjects, during the middle age group.

Usefulness of blink reflex in hypothyroid patients with or without polyneuropathy: a case control study.

Central nervous system (CNS) dysfunction is an important consequence of thyroid deficiency. Cranial nerves are frequently affected in hypothyroid process. On routine nerve conduction studies, symptomatic peripheral and cranial neuropathy can be detected, however, diagnosing subclinical cranial neuropathy pose the major problem. Blink reflex (BR), has been shown to be an effective method for revealing subclinical involvement of cranial nerves in generalized neuropathies. The present study was undertaken to evaluate the efficacy of BR as a method for early diagnosis of subclinical cranial neuropathy in hypothyroid patients with or without overt peripheral polyneuropathy. A case control study was conducted on 150 subjects aged 18 years and above (100 controls, 50 cases). A routine nerve conduction study and BR evaluation was done in all the subjects. We found abnormal BR response in 50% of hypothyroid patients studied. In hypothyroid patient without polyneuropathy R1 latency was significantly prolonged (P < 0.05 Vs control). Ipsilateral and contralateral R2 latencies were significantly prolonged in hypothyroid cases with or without polyneuropathy on bilateral stimulation. Magnitude of prolongation was greater in with polyneuropathy group. In conclusion, study suggests that BR is a useful non-invasive method for the detection of clinically silent cranial nerve compromise in hypothyroid patients.

Influence of visual angle on pattern reversal visual evoked potentials.

PURPOSE: The aim of this study was to find whether the visual evoked potential (VEP) latencies and amplitude are altered with different visual angles in healthy adult volunteers or not and to determine the visual angle which is the optimum and most appropriate among a wide range of check sizes for the reliable interpretation of pattern reversal VEPs (PRVEPs). MATERIALS AND METHODS: The present study was conducted on 40 healthy volunteers. The subjects were divided into two groups. One group consisted of 20 individuals (nine males and 11 females) in the age range of 25-57 years and they were exposed to checks subtending a visual angle of 90, 120, and 180 minutes of arc. Another group comprised of 20 individuals (10 males and 10 females) in the age range of 36-60 years and they were subjected to checks subtending a visual angle of 15, 30, and 120 minutes of arc. The stimulus configuration comprised of the transient pattern reversal method in which a black and white checker board is generated (full field) on a VEP Monitor by an Evoked Potential Recorder (RMS EMG. EPMARK II). The statistical analysis was done by One Way Analysis of Variance (ANOVA) using EPI INFO 6. RESULTS: In Group I, the maximum (max.) P100 latency of 98.8 ± 4.7 and the max. P100 amplitude of 10.05 ± 3.1 µV was obtained with checks of 90 minutes. In Group II, the max. P100 latency of 105.19 ± 4.75 msec as well as the max. P100 amplitude of 8.23 ± 3.30 µV was obtained with 15 minutes. The min. P100 latency in both the groups was obtained with checks of 120 minutes while the min. P100 amplitude was obtained with 180 minutes. A statistically significant difference was derived between means of P100 latency for 15 and 30 minutes with reference to its value for 120 minutes and between the mean value of P100 amplitude for 120 minutes and that of 90 and 180 minutes. CONCLUSION: Altering the size of stimulus (visual angle) has an effect on the PRVEP parameters. Our study found that the 120 is the appropriate (and optimal) check size that can be used for accurate interpretation of PRVEPs. This will help in better assessment of the optic nerve function and integrity of anterior visual pathways.

Brainstem Auditory Evoked Potentials (BAEP)- A Pilot Study Conducted on Young Healthy Adults from Central India.

OBJECTIVE: To Evaluate I, II, III, IV, V wave latencies and I-III, III-V, I-V inter-peak latencies and V/I wave amplitude ratio in Normal subjects in Central India. METHODS: We recorded BAEP from 50 healthy normal subjects from the community of same sex and geographical setup. The absolute, interpeak and wave V/I amplitude ratio were measurement and recording was done using RMS EMG EP MARK II machine manufactured by RMS recorders and Medicare system, Chandigarh. RESULT: Absolute, interpeak and wave V/I amplitude ratio were measured in normal subjects and compared with other previous studies. CONCLUSION: This study was conducted as exploratory pilot study only on male healthy controls. Since, the study conducted in different regions, there are some differences in the latencies and interpeak latencies and amplitude ratio but they are within range, so reference range of this study can be used for future studies in this Wardha region of Central India.

Correlation of pattern reversal visual evoked potential parameters with the pattern standard deviation in primary open angle glaucoma.

AIM: To evaluate whether glaucomatous visual field defect particularly the pattern standard deviation (PSD) of Humphrey visual field could be associated with visual evoked potential (VEP) parameters of patients having primary open angle glaucoma (POAG). METHODS: Visual field by Humphrey perimetry and simultaneous recordings of pattern reversal visual evoked potential (PRVEP) were assessed in 100 patients with POAG. The stimulus configuration for VEP recordings consisted of the transient pattern reversal method in which a black and white checker board pattern was generated (full field) and displayed on VEP monitor (colour 14″) by an electronic pattern regenerator inbuilt in an evoked potential recorder (RMS EMG EP MARK II). RESULTS: The results of our study indicate that there is a highly significant (P<0.001) negative correlation of P100 amplitude and a statistically significant (P<0.05) positive correlation of N70 latency, P100 latency and N155 latency with the PSD of Humphrey visual field in the subjects of POAG in various age groups as evaluated by Student's t-test. CONCLUSION: Prolongation of VEP latencies were mirrored by a corresponding increase of PSD values. Conversely, as PSD increases the magnitude of VEP excursions were found to be diminished.

Association of obesity and leptin with insulin resistance in type 2 diabetes mellitus in Indian population.

Obesity and diabetes mellitus are two modern epidemics. But their interrelationship is debated. Here we explored the probable association among obesity, leptin and insulin resistance in type 2 diabetes mellitus. 60 recent onset (< 5 years) diabetics and age-sex matched 33 non diabetic controls were assessed for physical and chemical parameters like Body Mass Index, abdominal circumference, waist/hip ratio, fasting blood glucose, insulin and leptin. Degree of insulin resistance was calculated by HOMA-IR method (Homeostatic Model Assessment). All the physical parameters showed positive correlation with leptin and the HOMA-IR score, strength of association being highest between insulin resistance and abdominal circumference. Leptin and insulin resistance showed no correlation. Findings were lower in controls. Study concluded that, obesity mainly central type might be responsible for insulin resistance in type 2 diabetes mellitus where as leptin, a potential marker for obesity, may not. This perhaps points towards the multifactorial causation of insulin resistance in type 2 diabetes mellitus.