Validation of a temperature prediction model for heat deaths in undocumented border crossers.

Heat exposure is a leading cause of death in undocumented border crossers along the Arizona-Mexico border. We performed a validation study of a weather prediction model that predicts the probability of heat related deaths among undocumented border crossers. We analyzed a medical examiner registry cohort of undocumented border crosser heat- related deaths from January 1, 2002 to August 31, 2009 and used logistic regression to model the probability of one or more heat deaths on a given day using daily high temperature (DHT) as the predictor. At a critical threshold DHT of 40 °C, the probability of at least one heat death was 50 %. The probability of a heat death along the Arizona-Mexico border for suspected undocumented border crossers is strongly associated with ambient temperature. These results can be used in prevention and response efforts to assess the daily risk of deaths among undocumented border crossers in the region.

Polymorphic microsatellite loci from northern and Mexican corn rootworms (Insecta: Coleoptera: Chrysomelidae) and cross-amplification with other Diabrotica spp.

The northern corn rootworm (Diabrotica barberi) and Mexican corn rootworm (Diabrotica virgifera zeae) are significant agricultural pests. For the northern corn rootworm, and to a lesser extent, the Mexican corn rootworm, high resolution molecular markers are needed. Here we present 14 polymorphic microsatellite loci isolated from libraries constructed using pooled northern and Mexican corn rootworm genomic DNA. Polymorphism in other Diabrotica, including the banded cucumber beetle, southern corn rootworm and western corn rootworm, is described.

Darwinian natural selection for orange bioluminescent color in a Jamaican click beetle.

The Jamaican click beetle Pyrophorus plagiophthalamus (Coleoptera: Elateridae) is unique among all bioluminescent organisms in displaying a striking light color polymorphism [Biggley, W. H., Lloyd, J. E. & Seliger, H. H. (1967) J. Gen. Physiol. 50, 1681-1692]. Beetles on the island vary in the color of their ventral light organs from yellow-green to orange and their dorsal organs from green to yellow-green. The genetic basis for the color variation involves specific amino acid substitutions in the enzyme luciferase. Here, we show that dorsal and ventral light color in P. plagiophthalamus are under separate genetic control, we resolve the allelic basis for color variation, and, through analyses of luciferase sequence variation, we demonstrate that natural selection has produced a long-term adaptive trend for longer wavelength (more orange) ventral light on Jamaica. Our results constitute a novel example connecting the selective fixation of specific nucleotides in nature to their precisely determined phenotypic effects. We also present evidence suggesting that a recently derived ventral orange luciferase allele on the island has deterministically increased in frequency. Thus, the current luciferase polymorphism for P. plagiophthalamus appears to be mirroring the long-term anagenic trend on Jamaica, revealing a possible ongoing adaptive color transition in progress.